TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Opačić, Miloš
AU  - Bajčetić, Milica
AU  - Mandic, Vesna
AU  - Maglic, Dragana
AU  - Miković, Zeljko
AU  - Spasojević, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1316
AB  - Oxidative status of maternal blood represents an important parameter of pregnancy that is involved in both, regulation of physiological processes and (if significantly altered) development of different pregnancy complications. Inherited thrombophilias represent genetic disorders that increase the risk of thromboembolism in pregnancy. Little is known about the impact of thrombophilia on the oxidative status of maternal blood. In this study, we analyzed oxidative status of blood of 56 women with pregnancies burdened by inherited thrombophilias. The status was established at three different trimesters using biochemical assays and electrochemical measurements, and it was compared to 10 age- and trimester-matching controls. Activities of superoxide dismutase, catalase, and glutathione reductase in the 1(st)and the 2(nd)trimester of thrombophilic pregnancy were lower than controls. Also, there was less oxidation in the plasma, according to higher concentration of reduced thiols and lower oxidation-reduction potential. Therefore, it appears that thrombophilic mothers do not experience oxidative stress in the circulation in the first two trimesters. However, the rise in GPx, GR and SOD activities in the 3(rd)trimester of thrombophilic pregnancy implies that the risk of oxidative stress is increased during the late pregnancy. These results are important for developing antioxidative treatment that could tackle thrombophilia-related pregnancy complications.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Oxidative status of maternal blood in pregnancies burdened by inherited thrombophilias
IS  - 6
VL  - 15
DO  - 10.1371/journal.pone.0234253
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Opačić, Miloš and Bajčetić, Milica and Mandic, Vesna and Maglic, Dragana and Miković, Zeljko and Spasojević, Ivan",
year = "2020",
abstract = "Oxidative status of maternal blood represents an important parameter of pregnancy that is involved in both, regulation of physiological processes and (if significantly altered) development of different pregnancy complications. Inherited thrombophilias represent genetic disorders that increase the risk of thromboembolism in pregnancy. Little is known about the impact of thrombophilia on the oxidative status of maternal blood. In this study, we analyzed oxidative status of blood of 56 women with pregnancies burdened by inherited thrombophilias. The status was established at three different trimesters using biochemical assays and electrochemical measurements, and it was compared to 10 age- and trimester-matching controls. Activities of superoxide dismutase, catalase, and glutathione reductase in the 1(st)and the 2(nd)trimester of thrombophilic pregnancy were lower than controls. Also, there was less oxidation in the plasma, according to higher concentration of reduced thiols and lower oxidation-reduction potential. Therefore, it appears that thrombophilic mothers do not experience oxidative stress in the circulation in the first two trimesters. However, the rise in GPx, GR and SOD activities in the 3(rd)trimester of thrombophilic pregnancy implies that the risk of oxidative stress is increased during the late pregnancy. These results are important for developing antioxidative treatment that could tackle thrombophilia-related pregnancy complications.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Oxidative status of maternal blood in pregnancies burdened by inherited thrombophilias",
number = "6",
volume = "15",
doi = "10.1371/journal.pone.0234253"
}

Bogdanović Pristov, J., Opačić, M., Bajčetić, M., Mandic, V., Maglic, D., Miković, Z.,& Spasojević, I.. (2020). Oxidative status of maternal blood in pregnancies burdened by inherited thrombophilias. in PLoS One
Public Library Science, San Francisco., 15(6).
https://doi.org/10.1371/journal.pone.0234253

Bogdanović Pristov J, Opačić M, Bajčetić M, Mandic V, Maglic D, Miković Z, Spasojević I. Oxidative status of maternal blood in pregnancies burdened by inherited thrombophilias. in PLoS One. 2020;15(6).
doi:10.1371/journal.pone.0234253 .

Bogdanović Pristov, Jelena, Opačić, Miloš, Bajčetić, Milica, Mandic, Vesna, Maglic, Dragana, Miković, Zeljko, Spasojević, Ivan, "Oxidative status of maternal blood in pregnancies burdened by inherited thrombophilias" in PLoS One, 15, no. 6 (2020),
https://doi.org/10.1371/journal.pone.0234253 . .

TY  - CONF
AU  - Savic, Danijela
AU  - Opačić, Miloš
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Bascarevic, Vladimir
AU  - Raičević, Savo
AU  - Savić, Slobodan
AU  - Živin, Marko
AU  - Šelih, Vid Simon
AU  - Spasic, Snezana
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2961
AB  - The accumulating evidence on the relation between the disturbed metal homeostasis and epilepsy urges the need for data regarding the total metal concentrations, as well as metal distribution in the brain itself, in order to indicate where to direct the potential therapy, to metal supplementation or chelation. This paper summarizes our results on the measurements of some important essential metals in hippocampi of patients with mesial temporal lobe epilepsy (mTLE) who underwent amigdalohippocampectomy. The key findings point out that levels of copper and manganese are deficient in hippocampi of mTLE patients, and that their concentrations correlated positively with neuronal loss in affected regions of sclerotic hippocampus. In addition, the Cu concentration was decreased in the areas of total neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor deficient compared to control. Our results contribute to deeper insight of metals biology in the epilepsy and may represent the initial point of new and non-invasive therapy of drug resistant epilepsy.
PB  - Biohemijsko društvo Srbije
C3  - Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia
T1  - Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy
EP  - 112
SP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2961
ER  - 

@conference{
author = "Savic, Danijela and Opačić, Miloš and Ristic, Aleksandar J and Sokic, Dragoslav and Bascarevic, Vladimir and Raičević, Savo and Savić, Slobodan and Živin, Marko and Šelih, Vid Simon and Spasic, Snezana and Spasojević, Ivan",
year = "2018",
abstract = "The accumulating evidence on the relation between the disturbed metal homeostasis and epilepsy urges the need for data regarding the total metal concentrations, as well as metal distribution in the brain itself, in order to indicate where to direct the potential therapy, to metal supplementation or chelation. This paper summarizes our results on the measurements of some important essential metals in hippocampi of patients with mesial temporal lobe epilepsy (mTLE) who underwent amigdalohippocampectomy. The key findings point out that levels of copper and manganese are deficient in hippocampi of mTLE patients, and that their concentrations correlated positively with neuronal loss in affected regions of sclerotic hippocampus. In addition, the Cu concentration was decreased in the areas of total neuronal loss. Iron and zinc total hippocampal levels were neither accumulated nor deficient compared to control. Our results contribute to deeper insight of metals biology in the epilepsy and may represent the initial point of new and non-invasive therapy of drug resistant epilepsy.",
publisher = "Biohemijsko društvo Srbije",
journal = "Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia",
title = "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy",
pages = "112-105",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2961"
}

Savic, D., Opačić, M., Ristic, A. J., Sokic, D., Bascarevic, V., Raičević, S., Savić, S., Živin, M., Šelih, V. S., Spasic, S.,& Spasojević, I.. (2018). Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia
Biohemijsko društvo Srbije., 105-112.
https://hdl.handle.net/21.15107/rcub_rimsi_2961

Savic D, Opačić M, Ristic AJ, Sokic D, Bascarevic V, Raičević S, Savić S, Živin M, Šelih VS, Spasic S, Spasojević I. Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia. 2018;:105-112.
https://hdl.handle.net/21.15107/rcub_rimsi_2961 .

Savic, Danijela, Opačić, Miloš, Ristic, Aleksandar J, Sokic, Dragoslav, Bascarevic, Vladimir, Raičević, Savo, Savić, Slobodan, Živin, Marko, Šelih, Vid Simon, Spasic, Snezana, Spasojević, Ivan, "Distribution and role of metals in sclerotic hippocampi of patients with mesial temporal lobe epilepsy" in Serbian Biochemical Society Eighth Conference with international participation, “Coordination in Biochemistry and Life”, University of Novi Sad – Rectorate Hall, 16.11.2018. Novi Sad, Serbia (2018):105-112,
https://hdl.handle.net/21.15107/rcub_rimsi_2961 .

TY  - JOUR
AU  - Opačić, Miloš
AU  - Stević, Zorica D
AU  - Baščarević, Vladimir
AU  - Zivić, Miroslav
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1115
AB  - The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 00, 000-000.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Antioxidants & Redox Signaling
T1  - Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?
EP  - 1575
IS  - 17
SP  - 1570
VL  - 28
DO  - 10.1089/ars.2017.7433
ER  - 

@article{
author = "Opačić, Miloš and Stević, Zorica D and Baščarević, Vladimir and Zivić, Miroslav and Spasić, Mihajlo and Spasojević, Ivan",
year = "2018",
abstract = "The monitoring of progression in amyotrophic lateral sclerosis (ALS) relies on clinical outcome measures that take months to interpret, such as revised ALS functional rating scale (ALSFRS-R) score, with no approved biomarkers. A number of clinical studies have documented the involvement of oxidative stress in ALS pathology. Pertinent to this, we propose to evaluate oxidation-reduction potential (ORP) of cerebrospinal fluid (CSF) as a potential indicator of ALS progression. The case-control study included 24 patients with neurological non-neurodegenerative disorders (controls) and 82 ALS patients with different degrees of disease (ALSFRS-R score: 21-47). ORP was significantly higher in ALS patients than controls. It was not dependent on age or gender. A strong negative correlation was found between ORP and ALSFRS-R score for all patients and patients with spinal onset. In other words, ORP increased with ALS progression. No correlation was found for the subset of patients with bulbar onset, most likely because of the physical distance between neurodegenerative loci and the site of CSF collection. These results lead to the hypothesis that ORP of CSF has a potential as monitoring biomarker in ALS, particularly in the cohort of patients with spinal onset. Antioxid. Redox Signal. 00, 000-000.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Antioxidants & Redox Signaling",
title = "Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?",
pages = "1575-1570",
number = "17",
volume = "28",
doi = "10.1089/ars.2017.7433"
}

Opačić, M., Stević, Z. D., Baščarević, V., Zivić, M., Spasić, M.,& Spasojević, I.. (2018). Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?. in Antioxidants & Redox Signaling
Mary Ann Liebert, Inc, New Rochelle., 28(17), 1570-1575.
https://doi.org/10.1089/ars.2017.7433

Opačić M, Stević ZD, Baščarević V, Zivić M, Spasić M, Spasojević I. Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?. in Antioxidants & Redox Signaling. 2018;28(17):1570-1575.
doi:10.1089/ars.2017.7433 .

Opačić, Miloš, Stević, Zorica D, Baščarević, Vladimir, Zivić, Miroslav, Spasić, Mihajlo, Spasojević, Ivan, "Can Oxidation-Reduction Potential of Cerebrospinal Fluid Be a Monitoring Biomarker in Amyotrophic Lateral Sclerosis?" in Antioxidants & Redox Signaling, 28, no. 17 (2018):1570-1575,
https://doi.org/10.1089/ars.2017.7433 . .

TY  - JOUR
AU  - Bozic, Bojana
AU  - Korać Jačić, Jelena
AU  - Stanković, Dalibor M.
AU  - Stanić, Marina
AU  - Romanović, Mima
AU  - Bogdanović Pristov, Jelena
AU  - Spasic, Snežana
AU  - Popovic-Bijelic, Ana
AU  - Spasojević, Ivan
AU  - Bajčetić, Milica
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1111
AB  - An increase in the copper pool in body fluids has been related to a number of pathological conditions, including infections. Copper ions may affect antibiotics via the formation of coordination bonds and/or redox reactions. Herein, we analyzed the interactions of Cu2+ with eight beta-lactam antibiotics using UV-Vis spectrophotometry, EPR spectroscopy, and electrochemical methods. Penicillin G did not show any detectable interactions with Cu2+. Ampicillin, amoxicillin and cephalexin formed stable colored complexes with octahedral coordination environment of Cu2+ with tetragonal distortion, and primary amine group as the site of coordinate bond formation. These beta-lactams increased the solubility of Cu2+ in the phosphate buffer. Ceftazidime and Cu2+ formed a complex with a similar geometry and gave rise to an organic radical. Ceftriaxone-Cu2+ complex appears to exhibit different geometry. All complexes showed 1: 1 stoichiometry. Cefaclor reduced Cu2+ to Cu1+ that further reacted with molecular oxygen to produce hydrogen peroxide. Finally, meropenem underwent degradation in the presence of copper. The analysis of activity against Escherichia coli and Staphylococcus aureus showed that the effects of meropenem, amoxicillin, ampicillin, and ceftriaxone were significantly hindered in the presence of copper ions. The interactions with copper ions should be taken into account regarding the problem of antibiotic resistance and in the selection of the most efficient antimicrobial therapy for patients with altered copper homeostasis.
PB  - Elsevier Science Inc, New York
T2  - Free Radical Biology and Medicine
T1  - Coordination and redox interactions of beta-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity
EP  - 285
SP  - 279
VL  - 129
DO  - 10.1016/j.freeradbiomed.2018.09.038
ER  - 

@article{
author = "Bozic, Bojana and Korać Jačić, Jelena and Stanković, Dalibor M. and Stanić, Marina and Romanović, Mima and Bogdanović Pristov, Jelena and Spasic, Snežana and Popovic-Bijelic, Ana and Spasojević, Ivan and Bajčetić, Milica",
year = "2018",
abstract = "An increase in the copper pool in body fluids has been related to a number of pathological conditions, including infections. Copper ions may affect antibiotics via the formation of coordination bonds and/or redox reactions. Herein, we analyzed the interactions of Cu2+ with eight beta-lactam antibiotics using UV-Vis spectrophotometry, EPR spectroscopy, and electrochemical methods. Penicillin G did not show any detectable interactions with Cu2+. Ampicillin, amoxicillin and cephalexin formed stable colored complexes with octahedral coordination environment of Cu2+ with tetragonal distortion, and primary amine group as the site of coordinate bond formation. These beta-lactams increased the solubility of Cu2+ in the phosphate buffer. Ceftazidime and Cu2+ formed a complex with a similar geometry and gave rise to an organic radical. Ceftriaxone-Cu2+ complex appears to exhibit different geometry. All complexes showed 1: 1 stoichiometry. Cefaclor reduced Cu2+ to Cu1+ that further reacted with molecular oxygen to produce hydrogen peroxide. Finally, meropenem underwent degradation in the presence of copper. The analysis of activity against Escherichia coli and Staphylococcus aureus showed that the effects of meropenem, amoxicillin, ampicillin, and ceftriaxone were significantly hindered in the presence of copper ions. The interactions with copper ions should be taken into account regarding the problem of antibiotic resistance and in the selection of the most efficient antimicrobial therapy for patients with altered copper homeostasis.",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Coordination and redox interactions of beta-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity",
pages = "285-279",
volume = "129",
doi = "10.1016/j.freeradbiomed.2018.09.038"
}

Bozic, B., Korać Jačić, J., Stanković, D. M., Stanić, M., Romanović, M., Bogdanović Pristov, J., Spasic, S., Popovic-Bijelic, A., Spasojević, I.,& Bajčetić, M.. (2018). Coordination and redox interactions of beta-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 129, 279-285.
https://doi.org/10.1016/j.freeradbiomed.2018.09.038

Bozic B, Korać Jačić J, Stanković DM, Stanić M, Romanović M, Bogdanović Pristov J, Spasic S, Popovic-Bijelic A, Spasojević I, Bajčetić M. Coordination and redox interactions of beta-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity. in Free Radical Biology and Medicine. 2018;129:279-285.
doi:10.1016/j.freeradbiomed.2018.09.038 .

Bozic, Bojana, Korać Jačić, Jelena, Stanković, Dalibor M., Stanić, Marina, Romanović, Mima, Bogdanović Pristov, Jelena, Spasic, Snežana, Popovic-Bijelic, Ana, Spasojević, Ivan, Bajčetić, Milica, "Coordination and redox interactions of beta-lactam antibiotics with Cu2+ in physiological settings and the impact on antibacterial activity" in Free Radical Biology and Medicine, 129 (2018):279-285,
https://doi.org/10.1016/j.freeradbiomed.2018.09.038 . .

TY  - JOUR
AU  - Opačić, Miloš
AU  - Ristic, Aleksandar J
AU  - Savić, Danijela Z
AU  - Selih, Vid Simon
AU  - Zivin, Marko
AU  - Sokic, Dragoslav
AU  - Raicević, Savo
AU  - Baščarević, Vladimir
AU  - Spasojević, Ivan
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1080
AB  - The loss of metal homeostasis has been implicated in the pathophysiology of mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). Here we applied laser ablation inductively coupled plasma mass spectrometry imaging to establish the spatial distribution of Zn, Fe, Cu and Mn in coronal sections of hippocampi of four patients with drug-resistant mTLE-HS who underwent amygdalohippocampectomy. Detailed maps of the metal concentrations in the different morphological areas/layers were built and analyzed. The highest level of Zn (>20 mu g g(-1)) was found in mossy fiber-rich regions -cornu ammonis field 4 (CA4), gyrus dentatus, and CA3. The distribution of Fe appears to reflect the routes of the main intrahippocampal blood vessels. The highest concentrations of Cu (410 mg g(-1)) and Mn (415 mu g g(-1)) were observed in regions/layers with neuron somata - subiculum, CA4, gyrus dentatus, and stratum pyramidale (SPy) in CA1 and CA2. Alveus and other regions with axons and dendrites generally showed lower levels of Zn, Cu, and Mn. The Cu concentration was decreased in the areas of total neuronal loss in SPy in CA1 (9.73 +/- 0.91 mu g g(-1)), compared to the subiculum (13.32 +/- .29 mu g g(-1); p = 0.043). The Cu and Mn concentrations correlated positively with neuron density in the SPy in CA1 (R = 0.629, p o 0.001; and R = 0.391, p = 0.004). These results provide a deeper insight into hippocampal metabolism of metals, and pave the road for identifying the components of the mechanism of epileptogenesis among Cu and Mn transporters and metalloproteins.
PB  - Royal Soc Chemistry, Cambridge
T2  - Metallomics
T1  - Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy
EP  - 148
IS  - 2
SP  - 141
VL  - 9
DO  - 10.1039/c6mt00293e
ER  - 

@article{
author = "Opačić, Miloš and Ristic, Aleksandar J and Savić, Danijela Z and Selih, Vid Simon and Zivin, Marko and Sokic, Dragoslav and Raicević, Savo and Baščarević, Vladimir and Spasojević, Ivan",
year = "2017",
abstract = "The loss of metal homeostasis has been implicated in the pathophysiology of mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). Here we applied laser ablation inductively coupled plasma mass spectrometry imaging to establish the spatial distribution of Zn, Fe, Cu and Mn in coronal sections of hippocampi of four patients with drug-resistant mTLE-HS who underwent amygdalohippocampectomy. Detailed maps of the metal concentrations in the different morphological areas/layers were built and analyzed. The highest level of Zn (>20 mu g g(-1)) was found in mossy fiber-rich regions -cornu ammonis field 4 (CA4), gyrus dentatus, and CA3. The distribution of Fe appears to reflect the routes of the main intrahippocampal blood vessels. The highest concentrations of Cu (410 mg g(-1)) and Mn (415 mu g g(-1)) were observed in regions/layers with neuron somata - subiculum, CA4, gyrus dentatus, and stratum pyramidale (SPy) in CA1 and CA2. Alveus and other regions with axons and dendrites generally showed lower levels of Zn, Cu, and Mn. The Cu concentration was decreased in the areas of total neuronal loss in SPy in CA1 (9.73 +/- 0.91 mu g g(-1)), compared to the subiculum (13.32 +/- .29 mu g g(-1); p = 0.043). The Cu and Mn concentrations correlated positively with neuron density in the SPy in CA1 (R = 0.629, p o 0.001; and R = 0.391, p = 0.004). These results provide a deeper insight into hippocampal metabolism of metals, and pave the road for identifying the components of the mechanism of epileptogenesis among Cu and Mn transporters and metalloproteins.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Metallomics",
title = "Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy",
pages = "148-141",
number = "2",
volume = "9",
doi = "10.1039/c6mt00293e"
}

Opačić, M., Ristic, A. J., Savić, D. Z., Selih, V. S., Zivin, M., Sokic, D., Raicević, S., Baščarević, V.,& Spasojević, I.. (2017). Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Metallomics
Royal Soc Chemistry, Cambridge., 9(2), 141-148.
https://doi.org/10.1039/c6mt00293e

Opačić M, Ristic AJ, Savić DZ, Selih VS, Zivin M, Sokic D, Raicević S, Baščarević V, Spasojević I. Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy. in Metallomics. 2017;9(2):141-148.
doi:10.1039/c6mt00293e .

Opačić, Miloš, Ristic, Aleksandar J, Savić, Danijela Z, Selih, Vid Simon, Zivin, Marko, Sokic, Dragoslav, Raicević, Savo, Baščarević, Vladimir, Spasojević, Ivan, "Metal maps of sclerotic hippocampi of patients with mesial temporal lobe epilepsy" in Metallomics, 9, no. 2 (2017):141-148,
https://doi.org/10.1039/c6mt00293e . .

TY  - JOUR
AU  - Bozic, Bojana
AU  - Korać Jačić, Jelena
AU  - Stanković, Dalibor M.
AU  - Stanić, Marina
AU  - Popovic-Bijelic, Ana
AU  - Bogdanović Pristov, Jelena
AU  - Spasojević, Ivan
AU  - Bajčetić, Milica
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1044
AB  - Toxic effects of unconjugated bilirubin (BR) in neonatal hyperbilirubinemia have been related to redox and/or coordinate interactions with Cu2+. However, the development and mechanisms of such interactions at physiological pH have not been resolved. This study shows that BR reduces Cu2+ to Cu1+ in 1:1 stoichiometry. Apparently, BR undergoes degradation, i.e. BR and Cu2+ do not form stable complexes. The binding of Cu2+ to inorganic phosphates, liposomal phosphate groups, or to chelating drug penicillamine, impedes redox interactions with BR. Cu1+ undergoes spontaneous oxidation by O-2 resulting in hydrogen peroxide accumulation and hydroxyl radical production. In relation to this, copper and BR induced synergistic oxidative/damaging effects on erythrocytes membrane, which were alleviated by penicillamine. The production of reactive oxygen species by BR and copper represents a plausible cause of BR toxic effects and cell damage in hyperbilirubinemia. Further examination of therapeutic potentials of copper chelators in the treatment of severe neonatal hyperbilirubinemia is needed.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - Mechanisms of redox interactions of bilirubin with copper and the effects of penicillamine
EP  - 134
SP  - 129
VL  - 278
DO  - 10.1016/j.cbi.2017.10.022
ER  - 

@article{
author = "Bozic, Bojana and Korać Jačić, Jelena and Stanković, Dalibor M. and Stanić, Marina and Popovic-Bijelic, Ana and Bogdanović Pristov, Jelena and Spasojević, Ivan and Bajčetić, Milica",
year = "2017",
abstract = "Toxic effects of unconjugated bilirubin (BR) in neonatal hyperbilirubinemia have been related to redox and/or coordinate interactions with Cu2+. However, the development and mechanisms of such interactions at physiological pH have not been resolved. This study shows that BR reduces Cu2+ to Cu1+ in 1:1 stoichiometry. Apparently, BR undergoes degradation, i.e. BR and Cu2+ do not form stable complexes. The binding of Cu2+ to inorganic phosphates, liposomal phosphate groups, or to chelating drug penicillamine, impedes redox interactions with BR. Cu1+ undergoes spontaneous oxidation by O-2 resulting in hydrogen peroxide accumulation and hydroxyl radical production. In relation to this, copper and BR induced synergistic oxidative/damaging effects on erythrocytes membrane, which were alleviated by penicillamine. The production of reactive oxygen species by BR and copper represents a plausible cause of BR toxic effects and cell damage in hyperbilirubinemia. Further examination of therapeutic potentials of copper chelators in the treatment of severe neonatal hyperbilirubinemia is needed.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "Mechanisms of redox interactions of bilirubin with copper and the effects of penicillamine",
pages = "134-129",
volume = "278",
doi = "10.1016/j.cbi.2017.10.022"
}

Bozic, B., Korać Jačić, J., Stanković, D. M., Stanić, M., Popovic-Bijelic, A., Bogdanović Pristov, J., Spasojević, I.,& Bajčetić, M.. (2017). Mechanisms of redox interactions of bilirubin with copper and the effects of penicillamine. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 278, 129-134.
https://doi.org/10.1016/j.cbi.2017.10.022

Bozic B, Korać Jačić J, Stanković DM, Stanić M, Popovic-Bijelic A, Bogdanović Pristov J, Spasojević I, Bajčetić M. Mechanisms of redox interactions of bilirubin with copper and the effects of penicillamine. in Chemico-Biological Interactions. 2017;278:129-134.
doi:10.1016/j.cbi.2017.10.022 .

Bozic, Bojana, Korać Jačić, Jelena, Stanković, Dalibor M., Stanić, Marina, Popovic-Bijelic, Ana, Bogdanović Pristov, Jelena, Spasojević, Ivan, Bajčetić, Milica, "Mechanisms of redox interactions of bilirubin with copper and the effects of penicillamine" in Chemico-Biological Interactions, 278 (2017):129-134,
https://doi.org/10.1016/j.cbi.2017.10.022 . .

TY  - JOUR
AU  - Martinov, Jelena
AU  - Krstic, Miodrag
AU  - Spasic, Snežana
AU  - Miletic, Srdjan
AU  - Stefanovic-Kojic, Jovana
AU  - Nikolić-Kokić, Aleksandra
AU  - Blagojević, Duško
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1050
AB  - Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.
PB  - Elsevier Science Bv, Amsterdam
T2  - Food Research International
T1  - Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus
EP  - 136
SP  - 132
VL  - 100
DO  - 10.1016/j.foodres.2017.08.040
ER  - 

@article{
author = "Martinov, Jelena and Krstic, Miodrag and Spasic, Snežana and Miletic, Srdjan and Stefanovic-Kojic, Jovana and Nikolić-Kokić, Aleksandra and Blagojević, Duško and Spasojević, Ivan and Spasić, Mihajlo",
year = "2017",
abstract = "Pectin is the main soluble fiber in apples or citruses. It may be fermented by gut microbiota to metabolites showing local intestinal and systemic effects. A wide range of beneficial effects of dietary pectin includes impacts on the redox milieu and microbiota profile. We prepared pectin-derived oligosaccharides (apple (APDO) and citrus) and polygalacturonic acid-derived oligosaccharides, using alkaline hydrolysis by hydrogen peroxide, and analyzed them by Foufier Transform Infrared spectrometry. Furthermore, we analyzed the effects of pectin derived oligosaccharides on hydroxyl radical (HO center dot)-generating Fenton reaction using electron paramagnetic resonance spin-trapping spectroscopy, and the effects on the growth of Escherichia coli and Staphylococcus minus in the presence of dietary-relevant HO center dot-generating system (iron + ascorbate). The oligosaccharides react with HO center dot radical to produce carbon dioxide radical anion (CO2 center dot (-)). A comparative analysis showed that APDO has the most prominent bacteriostatic effect. This might be at least partially related to the higher capacity of APDO to produce CO2 center dot (-), which specifically targets proteins and appears to have a longer lifetime and larger diffusion radius in biological systems compared to HO center dot.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Food Research International",
title = "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus",
pages = "136-132",
volume = "100",
doi = "10.1016/j.foodres.2017.08.040"
}

Martinov, J., Krstic, M., Spasic, S., Miletic, S., Stefanovic-Kojic, J., Nikolić-Kokić, A., Blagojević, D., Spasojević, I.,& Spasić, M.. (2017). Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International
Elsevier Science Bv, Amsterdam., 100, 132-136.
https://doi.org/10.1016/j.foodres.2017.08.040

Martinov J, Krstic M, Spasic S, Miletic S, Stefanovic-Kojic J, Nikolić-Kokić A, Blagojević D, Spasojević I, Spasić M. Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus. in Food Research International. 2017;100:132-136.
doi:10.1016/j.foodres.2017.08.040 .

Martinov, Jelena, Krstic, Miodrag, Spasic, Snežana, Miletic, Srdjan, Stefanovic-Kojic, Jovana, Nikolić-Kokić, Aleksandra, Blagojević, Duško, Spasojević, Ivan, Spasić, Mihajlo, "Apple pectin-derived oligosaccharides produce carbon dioxide radical anion in Fenton reaction and prevent growth of Escherichia coli and Staphylococcus aureus" in Food Research International, 100 (2017):132-136,
https://doi.org/10.1016/j.foodres.2017.08.040 . .

TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Bogdanović Pristov, Jelena
AU  - Popovic-Bijelic, Ana
AU  - Zakrzewska, Joanna
AU  - Stanić, Marina
AU  - Kalauzi, Aleksandar
AU  - Spasojević, Ivan
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/950
AB  - Iron-organic interactions are involved in a variety of environmental phenomena, including photo-redox reactions, iron cycling and bioavailability, as well as contaminant fate. In this study we examined UV-induced redox reactions of iron and indole in water. The presence of one indole in the irradiated system resulted in the presence of eight reduced ferric ions, not counting direct photolysis of Fe3+ complexes with OH-, which gives Fe2+ and hydroxyl radical (HO center dot) as products. The main mechanisms that contribute to indole-related Fe3+ reduction i.e. Fe2+ accumulation are: (i) HO center dot scavenging, which prevents oxidation of Fe2+ by HO center dot; (ii) oxidation of indole and its derivatives by excited ferric iron; (iii) reduction of ferric iron by excited indole (not present under UV-A). Hydrated electrons released by UV-B-excited indole play only a minor role in the reduction of iron. Indole-derived radicals emerged as byproducts of indole/iron photochemistry. H-1 NMR and low-T EPR spectroscopy showed that indole forms a weak low-symmetry complex with Fe3+. The strongest interactions between iron and pi-cloud in the indole ring are at positions 2, 3, and 7. The formation of complex promotes electron transfer from excited indole to Fe3+. Our findings are important for understanding the catalysis of photo-reduction of iron by heterocyclic aromatic pollutants, and for the development of protocols for indole processing in wastewaters.
PB  - Elsevier, Amsterdam
T2  - Applied Catalysis B-Environmental
T1  - Photo-redox reactions of indole and ferric iron in water
EP  - 180
SP  - 174
VL  - 185
DO  - 10.1016/j.apcatb.2015.12.018
ER  - 

@article{
author = "Milić Komić, Sonja and Bogdanović Pristov, Jelena and Popovic-Bijelic, Ana and Zakrzewska, Joanna and Stanić, Marina and Kalauzi, Aleksandar and Spasojević, Ivan",
year = "2016",
abstract = "Iron-organic interactions are involved in a variety of environmental phenomena, including photo-redox reactions, iron cycling and bioavailability, as well as contaminant fate. In this study we examined UV-induced redox reactions of iron and indole in water. The presence of one indole in the irradiated system resulted in the presence of eight reduced ferric ions, not counting direct photolysis of Fe3+ complexes with OH-, which gives Fe2+ and hydroxyl radical (HO center dot) as products. The main mechanisms that contribute to indole-related Fe3+ reduction i.e. Fe2+ accumulation are: (i) HO center dot scavenging, which prevents oxidation of Fe2+ by HO center dot; (ii) oxidation of indole and its derivatives by excited ferric iron; (iii) reduction of ferric iron by excited indole (not present under UV-A). Hydrated electrons released by UV-B-excited indole play only a minor role in the reduction of iron. Indole-derived radicals emerged as byproducts of indole/iron photochemistry. H-1 NMR and low-T EPR spectroscopy showed that indole forms a weak low-symmetry complex with Fe3+. The strongest interactions between iron and pi-cloud in the indole ring are at positions 2, 3, and 7. The formation of complex promotes electron transfer from excited indole to Fe3+. Our findings are important for understanding the catalysis of photo-reduction of iron by heterocyclic aromatic pollutants, and for the development of protocols for indole processing in wastewaters.",
publisher = "Elsevier, Amsterdam",
journal = "Applied Catalysis B-Environmental",
title = "Photo-redox reactions of indole and ferric iron in water",
pages = "180-174",
volume = "185",
doi = "10.1016/j.apcatb.2015.12.018"
}

Milić Komić, S., Bogdanović Pristov, J., Popovic-Bijelic, A., Zakrzewska, J., Stanić, M., Kalauzi, A.,& Spasojević, I.. (2016). Photo-redox reactions of indole and ferric iron in water. in Applied Catalysis B-Environmental
Elsevier, Amsterdam., 185, 174-180.
https://doi.org/10.1016/j.apcatb.2015.12.018

Milić Komić S, Bogdanović Pristov J, Popovic-Bijelic A, Zakrzewska J, Stanić M, Kalauzi A, Spasojević I. Photo-redox reactions of indole and ferric iron in water. in Applied Catalysis B-Environmental. 2016;185:174-180.
doi:10.1016/j.apcatb.2015.12.018 .

Milić Komić, Sonja, Bogdanović Pristov, Jelena, Popovic-Bijelic, Ana, Zakrzewska, Joanna, Stanić, Marina, Kalauzi, Aleksandar, Spasojević, Ivan, "Photo-redox reactions of indole and ferric iron in water" in Applied Catalysis B-Environmental, 185 (2016):174-180,
https://doi.org/10.1016/j.apcatb.2015.12.018 . .

TY  - JOUR
AU  - Marinković, Vesna
AU  - Rankovic-Janevski, Milica
AU  - Spasic, Snežana
AU  - Nikolić-Kokić, Aleksandra
AU  - Lugonja, Nikoleta
AU  - Djurović, Dijana
AU  - Miletic, Srdjan
AU  - Vrvić, Miroslav M.
AU  - Spasojević, Ivan
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/974
AB  - Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Pediatric Gastroenterology and Nutrition
T1  - Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage
EP  - 906
IS  - 6
SP  - 901
VL  - 62
DO  - 10.1097/MPG.0000000000001090
ER  - 

@article{
author = "Marinković, Vesna and Rankovic-Janevski, Milica and Spasic, Snežana and Nikolić-Kokić, Aleksandra and Lugonja, Nikoleta and Djurović, Dijana and Miletic, Srdjan and Vrvić, Miroslav M. and Spasojević, Ivan",
year = "2016",
abstract = "Objectives: Milk banks collect, pasteurize, and freeze/store human milk. The processing may alter redox properties of milk, but the effects have not been fully examined. Methods: We collected 10 mature milk and 10 colostrum samples and applied a battery of biochemical assays and electron paramagnetic resonance spectroscopy to inspect changes that milk undergoes with pasteurization and 30 days storage at -20 degrees C. Results: Pasteurization and storage of raw milk did not affect total nonenzymatic antioxidative capacity, but specific components and features were altered. Urate radical and ascorbyl radical emerge as products of exposure of milk to hydroxyl radical-generating system. Processing shifted the load of antioxidative activity from ascorbate to urate and lowered the capacity of milk to diminish hydroxyl radical. Pasteurization caused a significant drop in the activity of 2 major antioxidative enzymes-superoxide dismutase and glutathione peroxidase, whereas freezing/storage of raw milk affected only superoxide dismutase. Colostrum showed drastically higher total nonenzymatic antioxidative capacity, hydroxyl radical scavenging ability, and glutathione reductase activity compared with mature milk. Conclusions: Pasteurization and storage affect nonenzymatic and enzymatic antioxidative agents in human milk. It appears that nonenzymatic antioxidative systems in colostrum and milk are different. The effects of processing may be partially compensated by fortification/spiking with ascorbate before use.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Pediatric Gastroenterology and Nutrition",
title = "Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage",
pages = "906-901",
number = "6",
volume = "62",
doi = "10.1097/MPG.0000000000001090"
}

Marinković, V., Rankovic-Janevski, M., Spasic, S., Nikolić-Kokić, A., Lugonja, N., Djurović, D., Miletic, S., Vrvić, M. M.,& Spasojević, I.. (2016). Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage. in Journal of Pediatric Gastroenterology and Nutrition
Lippincott Williams & Wilkins, Philadelphia., 62(6), 901-906.
https://doi.org/10.1097/MPG.0000000000001090

Marinković V, Rankovic-Janevski M, Spasic S, Nikolić-Kokić A, Lugonja N, Djurović D, Miletic S, Vrvić MM, Spasojević I. Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage. in Journal of Pediatric Gastroenterology and Nutrition. 2016;62(6):901-906.
doi:10.1097/MPG.0000000000001090 .

Marinković, Vesna, Rankovic-Janevski, Milica, Spasic, Snežana, Nikolić-Kokić, Aleksandra, Lugonja, Nikoleta, Djurović, Dijana, Miletic, Srdjan, Vrvić, Miroslav M., Spasojević, Ivan, "Antioxidative Activity of Colostrum and Human Milk: Effects of Pasteurization and Storage" in Journal of Pediatric Gastroenterology and Nutrition, 62, no. 6 (2016):901-906,
https://doi.org/10.1097/MPG.0000000000001090 . .

TY  - JOUR
AU  - Spasojević, Ivan
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/998
AB  - Here, I address the topic of suitability for redox research of common settings in cell cultures. This is done through the prism of in vitro anticancer effects of vitamin C. Cell culture media show lower concentrations of iron and a higher level of oxygen compared to interstitial fluid. Such a setup promotes ascorbate-mediated production and accumulation of hydrogen peroxide, which efficiently kills a variety of cancer cell lines. However, the anticancer effects are annihilated if the iron level is corrected to mimic in vivo concentrations. It appears that the potential benefits of application of vitamin C in cancer treatment have been significantly overestimated. This might be true for other pro-oxidative agents as well, such as some (poly)phenols. We urgently need to establish medium formula and culture maintenance settings that are optimal for redox research.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Redox Report
T1  - What if cell culture media do not mimic in vivo redox settings?
EP  - 129
IS  - 3
SP  - 127
VL  - 21
DO  - 10.1179/1351000215Y.0000000036
ER  - 

@article{
author = "Spasojević, Ivan",
year = "2016",
abstract = "Here, I address the topic of suitability for redox research of common settings in cell cultures. This is done through the prism of in vitro anticancer effects of vitamin C. Cell culture media show lower concentrations of iron and a higher level of oxygen compared to interstitial fluid. Such a setup promotes ascorbate-mediated production and accumulation of hydrogen peroxide, which efficiently kills a variety of cancer cell lines. However, the anticancer effects are annihilated if the iron level is corrected to mimic in vivo concentrations. It appears that the potential benefits of application of vitamin C in cancer treatment have been significantly overestimated. This might be true for other pro-oxidative agents as well, such as some (poly)phenols. We urgently need to establish medium formula and culture maintenance settings that are optimal for redox research.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Redox Report",
title = "What if cell culture media do not mimic in vivo redox settings?",
pages = "129-127",
number = "3",
volume = "21",
doi = "10.1179/1351000215Y.0000000036"
}

Spasojević, I.. (2016). What if cell culture media do not mimic in vivo redox settings?. in Redox Report
Taylor & Francis Ltd, Abingdon., 21(3), 127-129.
https://doi.org/10.1179/1351000215Y.0000000036

Spasojević I. What if cell culture media do not mimic in vivo redox settings?. in Redox Report. 2016;21(3):127-129.
doi:10.1179/1351000215Y.0000000036 .

Spasojević, Ivan, "What if cell culture media do not mimic in vivo redox settings?" in Redox Report, 21, no. 3 (2016):127-129,
https://doi.org/10.1179/1351000215Y.0000000036 . .

TY  - JOUR
AU  - Milić Komić, Sonja
AU  - Bogdanović Pristov, Jelena
AU  - Mutavdžić, Dragosav
AU  - Savić, Aleksandar G
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/853
AB  - Herein we compared antioxidative activities (AA) of 25 free L-amino acids (FAA) against Fenton system-mediated hydroxyl radical (HO center dot) production in aqueous solution, and examined the relation between AA and a set of physicochemical properties. The tank order according -to AA was: Tip > notleucine > Phe, Lett > Ile > His >3,4-dihydrOxyphenylalanine, Arg > Val > Lys, Tyr, Pro > hydtoxyproline > alpha-aminobutyric acid > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp. Sulfur-containing FAA generated different secondary reactive products, which were discriminated by the means of electron paramagnetic resonance spin-trapping spectroscopy. AA showed a general positive correlation with hydrophobicity. However, when taken separately, uncharged FAA exhibited strong positive correlation of AA with hydrophobicity whereas charged FAA showed negative or no significant correlation depending on the scale applied. A general strong negative correlation was found between AA and polarity. Steric parameters and hydration numbers correlated positively with AA of rtoripolar side-chain FAA. In addition) a decrease of temperature which promotes hydrophobic hydration resulted in increased AA. This implies that HO-provoked oxidation of FAA is strongly affected by hydrophobic hydration. Our findings are important for the understanding of oxidation processes in natural and waste waters.
PB  - Amer Chemical Soc, Washington
T2  - Environmental Science & Technology
T1  - The Relationship of Physicochemical Properties to the Antioxidative Activity of Free Amino Acids in Fenton System
EP  - 4254
IS  - 7
SP  - 4245
VL  - 49
DO  - 10.1021/es5053396
ER  - 

@article{
author = "Milić Komić, Sonja and Bogdanović Pristov, Jelena and Mutavdžić, Dragosav and Savić, Aleksandar G and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Herein we compared antioxidative activities (AA) of 25 free L-amino acids (FAA) against Fenton system-mediated hydroxyl radical (HO center dot) production in aqueous solution, and examined the relation between AA and a set of physicochemical properties. The tank order according -to AA was: Tip > notleucine > Phe, Lett > Ile > His >3,4-dihydrOxyphenylalanine, Arg > Val > Lys, Tyr, Pro > hydtoxyproline > alpha-aminobutyric acid > Gln, Thr, Ser > Glu, Ala, Gly, Asn, Asp. Sulfur-containing FAA generated different secondary reactive products, which were discriminated by the means of electron paramagnetic resonance spin-trapping spectroscopy. AA showed a general positive correlation with hydrophobicity. However, when taken separately, uncharged FAA exhibited strong positive correlation of AA with hydrophobicity whereas charged FAA showed negative or no significant correlation depending on the scale applied. A general strong negative correlation was found between AA and polarity. Steric parameters and hydration numbers correlated positively with AA of rtoripolar side-chain FAA. In addition) a decrease of temperature which promotes hydrophobic hydration resulted in increased AA. This implies that HO-provoked oxidation of FAA is strongly affected by hydrophobic hydration. Our findings are important for the understanding of oxidation processes in natural and waste waters.",
publisher = "Amer Chemical Soc, Washington",
journal = "Environmental Science & Technology",
title = "The Relationship of Physicochemical Properties to the Antioxidative Activity of Free Amino Acids in Fenton System",
pages = "4254-4245",
number = "7",
volume = "49",
doi = "10.1021/es5053396"
}

Milić Komić, S., Bogdanović Pristov, J., Mutavdžić, D., Savić, A. G., Spasić, M.,& Spasojević, I.. (2015). The Relationship of Physicochemical Properties to the Antioxidative Activity of Free Amino Acids in Fenton System. in Environmental Science & Technology
Amer Chemical Soc, Washington., 49(7), 4245-4254.
https://doi.org/10.1021/es5053396

Milić Komić S, Bogdanović Pristov J, Mutavdžić D, Savić AG, Spasić M, Spasojević I. The Relationship of Physicochemical Properties to the Antioxidative Activity of Free Amino Acids in Fenton System. in Environmental Science & Technology. 2015;49(7):4245-4254.
doi:10.1021/es5053396 .

Milić Komić, Sonja, Bogdanović Pristov, Jelena, Mutavdžić, Dragosav, Savić, Aleksandar G, Spasić, Mihajlo, Spasojević, Ivan, "The Relationship of Physicochemical Properties to the Antioxidative Activity of Free Amino Acids in Fenton System" in Environmental Science & Technology, 49, no. 7 (2015):4245-4254,
https://doi.org/10.1021/es5053396 . .

TY  - JOUR
AU  - Ristic, Aleksandar J
AU  - Savić, Danijela Z
AU  - Sokic, Dragoslav
AU  - Bogdanović Pristov, Jelena
AU  - Nestorov, Jelena
AU  - Baščarević, Vladimir
AU  - Raicević, Savo
AU  - Savić, Slobodan
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/898
AB  - ObjectiveTo examine antioxidative system in hippocampi of patients with mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). MethodsActivity and levels of antioxidative enzymescatalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), manganese superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase (CuZnSOD)were assessed in hippocampi of nine pharmacoresistant mTLE-HS patients (mean age 37.7[standard deviation] 6.6years) who underwent amygdalohippocampectomy, and in 10 hippocampi obtained via autopsy from five neurologically intact controls (mean age 34.49.0years). Subfield and cellular (neuron/astrocyte) distribution of CAT, GPx, and MnSOD was analyzed in detail using immunohistochemical staining. ResultsSclerotic hippocampi showed drastically increased activity of hydrogen peroxide-removing enzymes, CAT (p lt 0.001), GPx (p lt 0.001), and GR (p lt 0.001), and significantly higher protein levels of CAT (p=0.006), GPx (p=0.040), GR (p=0.024), and MnSOD (p=0.004), compared to controls. CAT immunofluorescence was located mainly in neurons in both controls and HS. Control hippocampi showed GPx staining in blood vessels and CA neurons. In HS, GPx-rich loci, representing bundles of astrocytes, emerged in different hippocampal regions, whereas the number of GPx-positive vessels was drastically decreased. Neurons with abnormal morphology and strong MnSOD immunofluorescence were present in all neuronal layers in HS. Small autofluorescent deposits, most likely lipofuscin, were observed, along with astrogliosis, in CA1 in HS. SignificanceAntioxidative system is upregulated in HS. This documents, for the first time, that epileptogenic hippocampi are exposed to oxidative stress. Our findings provide a basis for understanding the potential involvement of redox alterations in the pathology of epilepsy, and may open new pharmacologic perspectives for mTLE-HS treatment.
PB  - Wiley, Hoboken
T2  - Epilepsia
T1  - Hippocampal antioxidative system in mesial temporal lobe epilepsy
EP  - 799
IS  - 5
SP  - 789
VL  - 56
DO  - 10.1111/epi.12981
ER  - 

@article{
author = "Ristic, Aleksandar J and Savić, Danijela Z and Sokic, Dragoslav and Bogdanović Pristov, Jelena and Nestorov, Jelena and Baščarević, Vladimir and Raicević, Savo and Savić, Slobodan and Spasojević, Ivan",
year = "2015",
abstract = "ObjectiveTo examine antioxidative system in hippocampi of patients with mesial temporal lobe epilepsy associated with hippocampal sclerosis (mTLE-HS). MethodsActivity and levels of antioxidative enzymescatalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), manganese superoxide dismutase (MnSOD), and copper-zinc superoxide dismutase (CuZnSOD)were assessed in hippocampi of nine pharmacoresistant mTLE-HS patients (mean age 37.7[standard deviation] 6.6years) who underwent amygdalohippocampectomy, and in 10 hippocampi obtained via autopsy from five neurologically intact controls (mean age 34.49.0years). Subfield and cellular (neuron/astrocyte) distribution of CAT, GPx, and MnSOD was analyzed in detail using immunohistochemical staining. ResultsSclerotic hippocampi showed drastically increased activity of hydrogen peroxide-removing enzymes, CAT (p lt 0.001), GPx (p lt 0.001), and GR (p lt 0.001), and significantly higher protein levels of CAT (p=0.006), GPx (p=0.040), GR (p=0.024), and MnSOD (p=0.004), compared to controls. CAT immunofluorescence was located mainly in neurons in both controls and HS. Control hippocampi showed GPx staining in blood vessels and CA neurons. In HS, GPx-rich loci, representing bundles of astrocytes, emerged in different hippocampal regions, whereas the number of GPx-positive vessels was drastically decreased. Neurons with abnormal morphology and strong MnSOD immunofluorescence were present in all neuronal layers in HS. Small autofluorescent deposits, most likely lipofuscin, were observed, along with astrogliosis, in CA1 in HS. SignificanceAntioxidative system is upregulated in HS. This documents, for the first time, that epileptogenic hippocampi are exposed to oxidative stress. Our findings provide a basis for understanding the potential involvement of redox alterations in the pathology of epilepsy, and may open new pharmacologic perspectives for mTLE-HS treatment.",
publisher = "Wiley, Hoboken",
journal = "Epilepsia",
title = "Hippocampal antioxidative system in mesial temporal lobe epilepsy",
pages = "799-789",
number = "5",
volume = "56",
doi = "10.1111/epi.12981"
}

Ristic, A. J., Savić, D. Z., Sokic, D., Bogdanović Pristov, J., Nestorov, J., Baščarević, V., Raicević, S., Savić, S.,& Spasojević, I.. (2015). Hippocampal antioxidative system in mesial temporal lobe epilepsy. in Epilepsia
Wiley, Hoboken., 56(5), 789-799.
https://doi.org/10.1111/epi.12981

Ristic AJ, Savić DZ, Sokic D, Bogdanović Pristov J, Nestorov J, Baščarević V, Raicević S, Savić S, Spasojević I. Hippocampal antioxidative system in mesial temporal lobe epilepsy. in Epilepsia. 2015;56(5):789-799.
doi:10.1111/epi.12981 .

Ristic, Aleksandar J, Savić, Danijela Z, Sokic, Dragoslav, Bogdanović Pristov, Jelena, Nestorov, Jelena, Baščarević, Vladimir, Raicević, Savo, Savić, Slobodan, Spasojević, Ivan, "Hippocampal antioxidative system in mesial temporal lobe epilepsy" in Epilepsia, 56, no. 5 (2015):789-799,
https://doi.org/10.1111/epi.12981 . .

TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijusković, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasic, Snežana
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/846
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
EP  - 23
SP  - 19
VL  - 51
DO  - 10.1016/j.niox.2015.09.008
ER  - 

@article{
author = "Bolic, Bojana and Mijusković, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasic, Snežana and Blagojević, Duško and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study",
pages = "23-19",
volume = "51",
doi = "10.1016/j.niox.2015.09.008"
}

Bolic, B., Mijusković, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasic, S., Blagojević, D., Spasić, M.,& Spasojević, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijusković A, Popovic-Bijelic A, Nikolić-Kokić A, Spasic S, Blagojević D, Spasić M, Spasojević I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23.
doi:10.1016/j.niox.2015.09.008 .

Bolic, Bojana, Mijusković, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasic, Snežana, Blagojević, Duško, Spasić, Mihajlo, Spasojević, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 . .

TY  - JOUR
AU  - Miljković, Đorđe
AU  - Blazevski, Jana
AU  - Petković, Filip
AU  - Djedović, Neda
AU  - Momcilović, Miljana
AU  - Stanisavljević, Suzana
AU  - Jevtic, Bojan
AU  - Mostarica-Stojković, Marija
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/891
AB  - Dimethyl fumarate (DMF), a new drug for multiple sclerosis (MS) treatment, acts against neuroinflammation via mechanisms that are triggered by adduct formation with thiol redox switches. Ethyl pyruvate (EP), an off-the-shelf agent, appears to be a redox analog of DMF, but its immunomodulatory properties have not been put into the context of MS therapy. In this article, we examined and compared the effects of EP and DMF on MS-relevant activity/functions of T cells, macrophages, microglia, and astrocytes. EP efficiently suppressed the release of MS signature cytokines, IFN-gamma and IL-17, from human PBMCs. Furthermore, the production of these cytokines was notably decreased in encephalitogenic T cells after in vivo application of EP to rats. Production of two other proinflammatory cytokines, IL-6 and TNF, and NO was suppressed by EP in macrophages and microglia. Reactive oxygen species production in macrophages, microglia activation, and the development of Ag-presenting phenotype in microglia and macrophages were constrained by EP. The release of IL-6 was reduced in astrocytes. Finally, EP inhibited the activation of transcription factor NF-kappa B in microglia and astrocytes. Most of these effects were also found for DMF, implying that EP and DMF share common targets and mechanisms of action. Importantly, EP had in vivo impact on experimental autoimmune encephalomyelitis, an animal model of MS. Treatment with EP resulted in delay and shortening of the first relapse, and lower clinical scores, whereas the second attack was annihilated. Further studies on the possibility to use EP as an MS therapeutic are warranted.
PB  - Amer Assoc Immunologists, Bethesda
T2  - Journal of Immunology
T1  - A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory Properties of Ethyl Pyruvate and Dimethyl Fumarate
EP  - 2503
IS  - 6
SP  - 2493
VL  - 194
DO  - 10.4049/jimmunol.1402302
ER  - 

@article{
author = "Miljković, Đorđe and Blazevski, Jana and Petković, Filip and Djedović, Neda and Momcilović, Miljana and Stanisavljević, Suzana and Jevtic, Bojan and Mostarica-Stojković, Marija and Spasojević, Ivan",
year = "2015",
abstract = "Dimethyl fumarate (DMF), a new drug for multiple sclerosis (MS) treatment, acts against neuroinflammation via mechanisms that are triggered by adduct formation with thiol redox switches. Ethyl pyruvate (EP), an off-the-shelf agent, appears to be a redox analog of DMF, but its immunomodulatory properties have not been put into the context of MS therapy. In this article, we examined and compared the effects of EP and DMF on MS-relevant activity/functions of T cells, macrophages, microglia, and astrocytes. EP efficiently suppressed the release of MS signature cytokines, IFN-gamma and IL-17, from human PBMCs. Furthermore, the production of these cytokines was notably decreased in encephalitogenic T cells after in vivo application of EP to rats. Production of two other proinflammatory cytokines, IL-6 and TNF, and NO was suppressed by EP in macrophages and microglia. Reactive oxygen species production in macrophages, microglia activation, and the development of Ag-presenting phenotype in microglia and macrophages were constrained by EP. The release of IL-6 was reduced in astrocytes. Finally, EP inhibited the activation of transcription factor NF-kappa B in microglia and astrocytes. Most of these effects were also found for DMF, implying that EP and DMF share common targets and mechanisms of action. Importantly, EP had in vivo impact on experimental autoimmune encephalomyelitis, an animal model of MS. Treatment with EP resulted in delay and shortening of the first relapse, and lower clinical scores, whereas the second attack was annihilated. Further studies on the possibility to use EP as an MS therapeutic are warranted.",
publisher = "Amer Assoc Immunologists, Bethesda",
journal = "Journal of Immunology",
title = "A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory Properties of Ethyl Pyruvate and Dimethyl Fumarate",
pages = "2503-2493",
number = "6",
volume = "194",
doi = "10.4049/jimmunol.1402302"
}

Miljković, Đ., Blazevski, J., Petković, F., Djedović, N., Momcilović, M., Stanisavljević, S., Jevtic, B., Mostarica-Stojković, M.,& Spasojević, I.. (2015). A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory Properties of Ethyl Pyruvate and Dimethyl Fumarate. in Journal of Immunology
Amer Assoc Immunologists, Bethesda., 194(6), 2493-2503.
https://doi.org/10.4049/jimmunol.1402302

Miljković Đ, Blazevski J, Petković F, Djedović N, Momcilović M, Stanisavljević S, Jevtic B, Mostarica-Stojković M, Spasojević I. A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory Properties of Ethyl Pyruvate and Dimethyl Fumarate. in Journal of Immunology. 2015;194(6):2493-2503.
doi:10.4049/jimmunol.1402302 .

Miljković, Đorđe, Blazevski, Jana, Petković, Filip, Djedović, Neda, Momcilović, Miljana, Stanisavljević, Suzana, Jevtic, Bojan, Mostarica-Stojković, Marija, Spasojević, Ivan, "A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory Properties of Ethyl Pyruvate and Dimethyl Fumarate" in Journal of Immunology, 194, no. 6 (2015):2493-2503,
https://doi.org/10.4049/jimmunol.1402302 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin-Dušić, Zorana
AU  - Spasojević, Ivan
AU  - Blagojević, Duško
AU  - Stević, Zorica D
AU  - Andjus, Pavle R.
AU  - Spasić, Mihajlo
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/902
AB  - In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - The effects of wild-type and mutant sod1 on smooth muscle contraction
EP  - 192
IS  - 1
SP  - 187
VL  - 67
DO  - 10.2298/ABS141006023N
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin-Dušić, Zorana and Spasojević, Ivan and Blagojević, Duško and Stević, Zorica D and Andjus, Pavle R. and Spasić, Mihajlo",
year = "2015",
abstract = "In this work we compared the mutated liver copper zinc-containing superoxide dismutase (SOD1) protein G93A of the transgenic rat model of familial amyotrophic lateral sclerosis (FALS), to wild-type (WT) rat SOD1. We examined their enzymatic activities and effects on isometric contractions of uteri of healthy virgin rats. G93A SOD1 showed a slightly higher activity than WT SOD1 and, in contrast to WT SOD1, G93A SOD1 did not induce smooth muscle relaxation. This result indicates that effects on smooth muscles are not related to SOD1 enzyme activity and suggest that heterodimers of G93A SOD1 form an ion-conducting pore that diminishes the relaxatory effects of SOD1. We propose that this type of pathogenic feedback affects neurons in FALS.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "The effects of wild-type and mutant sod1 on smooth muscle contraction",
pages = "192-187",
number = "1",
volume = "67",
doi = "10.2298/ABS141006023N"
}

Nikolić-Kokić, A., Oreščanin-Dušić, Z., Spasojević, I., Blagojević, D., Stević, Z. D., Andjus, P. R.,& Spasić, M.. (2015). The effects of wild-type and mutant sod1 on smooth muscle contraction. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 67(1), 187-192.
https://doi.org/10.2298/ABS141006023N

Nikolić-Kokić A, Oreščanin-Dušić Z, Spasojević I, Blagojević D, Stević ZD, Andjus PR, Spasić M. The effects of wild-type and mutant sod1 on smooth muscle contraction. in Archives of Biological Sciences. 2015;67(1):187-192.
doi:10.2298/ABS141006023N .

Nikolić-Kokić, Aleksandra, Oreščanin-Dušić, Zorana, Spasojević, Ivan, Blagojević, Duško, Stević, Zorica D, Andjus, Pavle R., Spasić, Mihajlo, "The effects of wild-type and mutant sod1 on smooth muscle contraction" in Archives of Biological Sciences, 67, no. 1 (2015):187-192,
https://doi.org/10.2298/ABS141006023N . .

TY  - JOUR
AU  - Bogosavljević, Vojislav
AU  - Bajčetić, Milica
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/823
AB  - Objectives: Comparison of redox conditions in malignant and benign tumours is essential for understanding the role of reactive oxygen species in the pathophysiology of aggressive cancer profiles. Here we compare antioxidative systems in highly malignant brain tumour - glioblastoma multiforme (GBM), and in meningioma, a benign brain tumour. Methods: Tumour tissues and blood of 67 GBM patients (mean age: 52.9 +/- 11.5 years) and 67 meningioma patients (59.2 +/- 10.2 years), and blood of 30 control subjects (50.8 +/- 12.8 years) were analysed via biochemical assays. Results: Components of glutathione system, which is responsible for H2O2 removal, showed lower activity/level in GBM: glutathione peroxidase (GBM: 9.90 +/- 0.22; meningioma: 11.78 +/- 0.23 U/mg of proteins; P  lt  0.001), glutathione reductase (GBM: 3.83 +/- 0.13; meningioma: 4.67 +/- 0.11 U/mg of proteins; P  lt  0.001), and glutathione (GBM: 6.70 +/- 0.12; meningioma: 7.58 +/- 0.14 mu mol/g of tissue; P  lt  0.001). In contrast, the rank order of glutathione reductase activity and glutathione level in erythrocytes was: GBM > meningioma > control. Superoxide dismutase and catalase activities were lower in the blood of cancer patients compared to controls. Discussion: Cells of malignant brain tumour show down-regulated antioxidative system which might result in increased levels of H2O2 compared to benign tumour tissue.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Redox Report
T1  - Comparative analysis of antioxidative systems in malignant and benign brain tumours
EP  - 74
IS  - 2
SP  - 69
VL  - 20
DO  - 10.1179/1351000214Y.0000000106
ER  - 

@article{
author = "Bogosavljević, Vojislav and Bajčetić, Milica and Spasojević, Ivan",
year = "2015",
abstract = "Objectives: Comparison of redox conditions in malignant and benign tumours is essential for understanding the role of reactive oxygen species in the pathophysiology of aggressive cancer profiles. Here we compare antioxidative systems in highly malignant brain tumour - glioblastoma multiforme (GBM), and in meningioma, a benign brain tumour. Methods: Tumour tissues and blood of 67 GBM patients (mean age: 52.9 +/- 11.5 years) and 67 meningioma patients (59.2 +/- 10.2 years), and blood of 30 control subjects (50.8 +/- 12.8 years) were analysed via biochemical assays. Results: Components of glutathione system, which is responsible for H2O2 removal, showed lower activity/level in GBM: glutathione peroxidase (GBM: 9.90 +/- 0.22; meningioma: 11.78 +/- 0.23 U/mg of proteins; P  lt  0.001), glutathione reductase (GBM: 3.83 +/- 0.13; meningioma: 4.67 +/- 0.11 U/mg of proteins; P  lt  0.001), and glutathione (GBM: 6.70 +/- 0.12; meningioma: 7.58 +/- 0.14 mu mol/g of tissue; P  lt  0.001). In contrast, the rank order of glutathione reductase activity and glutathione level in erythrocytes was: GBM > meningioma > control. Superoxide dismutase and catalase activities were lower in the blood of cancer patients compared to controls. Discussion: Cells of malignant brain tumour show down-regulated antioxidative system which might result in increased levels of H2O2 compared to benign tumour tissue.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Redox Report",
title = "Comparative analysis of antioxidative systems in malignant and benign brain tumours",
pages = "74-69",
number = "2",
volume = "20",
doi = "10.1179/1351000214Y.0000000106"
}

Bogosavljević, V., Bajčetić, M.,& Spasojević, I.. (2015). Comparative analysis of antioxidative systems in malignant and benign brain tumours. in Redox Report
Taylor & Francis Ltd, Abingdon., 20(2), 69-74.
https://doi.org/10.1179/1351000214Y.0000000106

Bogosavljević V, Bajčetić M, Spasojević I. Comparative analysis of antioxidative systems in malignant and benign brain tumours. in Redox Report. 2015;20(2):69-74.
doi:10.1179/1351000214Y.0000000106 .

Bogosavljević, Vojislav, Bajčetić, Milica, Spasojević, Ivan, "Comparative analysis of antioxidative systems in malignant and benign brain tumours" in Redox Report, 20, no. 2 (2015):69-74,
https://doi.org/10.1179/1351000214Y.0000000106 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin-Dušić, Zorana
AU  - Spasojević, Ivan
AU  - Slavić, Marija
AU  - Mijusković, Ana
AU  - Paskulin, Roman
AU  - Miljević, Cedo
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško P.
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/889
AB  - Ethnopharmacological relevance: Ibogaine is a naturally occurring alkaloid with psychotropic and metabotropic effects, derived from the bark of the root of the West African Tabernanthe iboga plant. The tribes of Kongo basin have been using iboga as a stimulant, for medicinal purposes, and in rite of passage ceremonies, for centuries. Besides, it has been found that this drug has anti-addictive effects. Aim of the study: Previous studies have demonstrated that ibogaine changed the quantity of ATP and energy related enzymes as well as the activity of antioxidant enzymes in cells thus altering redox equilibrium in a time manner. In this work, the mechanism of its action was further studied by measuring the effects of ibogaine in human erythrocytes in vitro on ATP liberation, membrane fluidity and antioxidant enzymes activity. Materials and methods: Heparinized human blood samples were incubated with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was separated by centrifugation and the levels of ATP and uric acid were measured 10 mm after the addition of ibogaine using standard kits. The activity of copper zinc superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were measured in erythrocytes after incubation period. The stability of SOD1 activity was further tested through in vitro incubation with H2O2 and scanning of its electrophoretic profiles. Membrane fluidity was determined using an electron paramagnetic resonance spin-labelling method. Results: Results showed that ibogaine treatment of erythrocytes in vitro increased ATP concentration in the blood plasma without changes in neither erythrocytes membrane fluidity nor uric acid concentration. lbogaine also increased SOD1 activity in erythrocytes at both doses applied here. Treatment with 20 mu M also elevated GR activity after in vitro incubation at 37 degrees C. Electrophoretic profiles revealed that incubation with ibogaine mitigates H2O2 mediated suppression of SOD1 activity. Conclusion: Some of the effects of ibogaine seem to be mediated through its influence on energy metabolism, redox active processes and the effects of discrete fluctuations of individual reactive oxygen species on different levels of enzyme activities. Overall, ibogaine acts as a pro-antioxidant by increasing activity of antioxidative enzymes and as an adaptagene in oxidative distress.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes
EP  - 70
SP  - 64
VL  - 164
DO  - 10.1016/j.jep.2015.01.037
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin-Dušić, Zorana and Spasojević, Ivan and Slavić, Marija and Mijusković, Ana and Paskulin, Roman and Miljević, Cedo and Spasić, Mihajlo and Blagojević, Duško P.",
year = "2015",
abstract = "Ethnopharmacological relevance: Ibogaine is a naturally occurring alkaloid with psychotropic and metabotropic effects, derived from the bark of the root of the West African Tabernanthe iboga plant. The tribes of Kongo basin have been using iboga as a stimulant, for medicinal purposes, and in rite of passage ceremonies, for centuries. Besides, it has been found that this drug has anti-addictive effects. Aim of the study: Previous studies have demonstrated that ibogaine changed the quantity of ATP and energy related enzymes as well as the activity of antioxidant enzymes in cells thus altering redox equilibrium in a time manner. In this work, the mechanism of its action was further studied by measuring the effects of ibogaine in human erythrocytes in vitro on ATP liberation, membrane fluidity and antioxidant enzymes activity. Materials and methods: Heparinized human blood samples were incubated with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was separated by centrifugation and the levels of ATP and uric acid were measured 10 mm after the addition of ibogaine using standard kits. The activity of copper zinc superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were measured in erythrocytes after incubation period. The stability of SOD1 activity was further tested through in vitro incubation with H2O2 and scanning of its electrophoretic profiles. Membrane fluidity was determined using an electron paramagnetic resonance spin-labelling method. Results: Results showed that ibogaine treatment of erythrocytes in vitro increased ATP concentration in the blood plasma without changes in neither erythrocytes membrane fluidity nor uric acid concentration. lbogaine also increased SOD1 activity in erythrocytes at both doses applied here. Treatment with 20 mu M also elevated GR activity after in vitro incubation at 37 degrees C. Electrophoretic profiles revealed that incubation with ibogaine mitigates H2O2 mediated suppression of SOD1 activity. Conclusion: Some of the effects of ibogaine seem to be mediated through its influence on energy metabolism, redox active processes and the effects of discrete fluctuations of individual reactive oxygen species on different levels of enzyme activities. Overall, ibogaine acts as a pro-antioxidant by increasing activity of antioxidative enzymes and as an adaptagene in oxidative distress.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes",
pages = "70-64",
volume = "164",
doi = "10.1016/j.jep.2015.01.037"
}

Nikolić-Kokić, A., Oreščanin-Dušić, Z., Spasojević, I., Slavić, M., Mijusković, A., Paskulin, R., Miljević, C., Spasić, M.,& Blagojević, D. P.. (2015). Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 164, 64-70.
https://doi.org/10.1016/j.jep.2015.01.037

Nikolić-Kokić A, Oreščanin-Dušić Z, Spasojević I, Slavić M, Mijusković A, Paskulin R, Miljević C, Spasić M, Blagojević DP. Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes. in Journal of Ethnopharmacology. 2015;164:64-70.
doi:10.1016/j.jep.2015.01.037 .

Nikolić-Kokić, Aleksandra, Oreščanin-Dušić, Zorana, Spasojević, Ivan, Slavić, Marija, Mijusković, Ana, Paskulin, Roman, Miljević, Cedo, Spasić, Mihajlo, Blagojević, Duško P., "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes" in Journal of Ethnopharmacology, 164 (2015):64-70,
https://doi.org/10.1016/j.jep.2015.01.037 . .

TY  - JOUR
AU  - Mojic, Marija
AU  - Bogdanović Pristov, Jelena
AU  - Maksimović-Ivanić, Danijela
AU  - Jones, David R
AU  - Stanić, Marina
AU  - Mijatović, Sanja
AU  - Spasojević, Ivan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/821
AB  - In vitro studies have shown that hydrogen peroxide (H2O2) produced by high-concentration ascorbate and cell culture medium iron efficiently kills cancer cells. This provided the rationale for clinical trials of high-dose intravenous ascorbate-based treatment for cancer. A drawback in all the in vitro studies was their failure to take into account the in vivo concentration of iron to supplement cell culture media which are characterized by low iron content. Here we showed, using two prostate cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the anticancer/cytotoxic effects of ascorbate are completely abolished by iron at physiological concentrations in cell culture medium and human plasma. A detailed examination of mechanisms showed that iron at physiological concentrations promotes both production and decomposition of H2O2. The latter is mediated by Fenton reaction and prevents H2O2 accumulation. The hydroxyl radical, which is produced in the Fenton reaction, is buffered by extracellular proteins, and could not affect intracellular targets like H2O2. These findings show that anticancer effects of ascorbate have been significantly overestimated in previous in vitro studies, and that common cell culture media might be unsuitable for redox research.
PB  - Nature Publishing Group, London
T2  - Scientific Reports
T1  - Extracellular iron diminishes anticancer effects of vitamin C: An in vitro study
VL  - 4
DO  - 10.1038/srep05955
ER  - 

@article{
author = "Mojic, Marija and Bogdanović Pristov, Jelena and Maksimović-Ivanić, Danijela and Jones, David R and Stanić, Marina and Mijatović, Sanja and Spasojević, Ivan",
year = "2014",
abstract = "In vitro studies have shown that hydrogen peroxide (H2O2) produced by high-concentration ascorbate and cell culture medium iron efficiently kills cancer cells. This provided the rationale for clinical trials of high-dose intravenous ascorbate-based treatment for cancer. A drawback in all the in vitro studies was their failure to take into account the in vivo concentration of iron to supplement cell culture media which are characterized by low iron content. Here we showed, using two prostate cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the anticancer/cytotoxic effects of ascorbate are completely abolished by iron at physiological concentrations in cell culture medium and human plasma. A detailed examination of mechanisms showed that iron at physiological concentrations promotes both production and decomposition of H2O2. The latter is mediated by Fenton reaction and prevents H2O2 accumulation. The hydroxyl radical, which is produced in the Fenton reaction, is buffered by extracellular proteins, and could not affect intracellular targets like H2O2. These findings show that anticancer effects of ascorbate have been significantly overestimated in previous in vitro studies, and that common cell culture media might be unsuitable for redox research.",
publisher = "Nature Publishing Group, London",
journal = "Scientific Reports",
title = "Extracellular iron diminishes anticancer effects of vitamin C: An in vitro study",
volume = "4",
doi = "10.1038/srep05955"
}

Mojic, M., Bogdanović Pristov, J., Maksimović-Ivanić, D., Jones, D. R., Stanić, M., Mijatović, S.,& Spasojević, I.. (2014). Extracellular iron diminishes anticancer effects of vitamin C: An in vitro study. in Scientific Reports
Nature Publishing Group, London., 4.
https://doi.org/10.1038/srep05955

Mojic M, Bogdanović Pristov J, Maksimović-Ivanić D, Jones DR, Stanić M, Mijatović S, Spasojević I. Extracellular iron diminishes anticancer effects of vitamin C: An in vitro study. in Scientific Reports. 2014;4.
doi:10.1038/srep05955 .

Mojic, Marija, Bogdanović Pristov, Jelena, Maksimović-Ivanić, Danijela, Jones, David R, Stanić, Marina, Mijatović, Sanja, Spasojević, Ivan, "Extracellular iron diminishes anticancer effects of vitamin C: An in vitro study" in Scientific Reports, 4 (2014),
https://doi.org/10.1038/srep05955 . .

TY  - JOUR
AU  - Ristic, Aleksandar J
AU  - Sokic, Dragoslav
AU  - Baščarević, Vladimir
AU  - Spasic, Snežana D
AU  - Vojvodic, Nikola M
AU  - Savić, Slobodan
AU  - Raicević, Savo
AU  - Kovacević, Masa
AU  - Savić, Danijela Z
AU  - Spasojević, Ivan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/791
AB  - An altered metal and electrolyte profile has been implicated in the pathologic mechanisms of chronic epilepsy; however, no study has comprehensively measured hippocampal concentrations of these elements in patients with mesial temporal lobe epilepsy and hippocampal sclerosis (mTLE-HS). We therefore analyzed hippocampi of 24 patients with drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior temporal lobe resection and amygdalohippocampectomy and 17 hippocampi obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years), using inductively coupled plasma optical emission spectrometry (ICP-OES). Epileptic hippocampi showed significantly lower concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control [C]: 3.57 +/- 0.33; p lt 0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409 +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61; p lt 0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/- 25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ in HS and controls. In summary, copper and manganese levels are deficient, whereas iron level is unchanged in hippocampi from patients with mTLE-HS. Our results provide a basis for understanding the potential involvement of different metals and electrolytes in the pathology of HS. A PowerPoint slide summarizing this article is available for download in the Supporting Information section .
PB  - Wiley-Blackwell, Hoboken
T2  - Epilepsia
T1  - Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy
EP  - E37
IS  - 5
SP  - E34
VL  - 55
DO  - 10.1111/epi.12593
ER  - 

@article{
author = "Ristic, Aleksandar J and Sokic, Dragoslav and Baščarević, Vladimir and Spasic, Snežana D and Vojvodic, Nikola M and Savić, Slobodan and Raicević, Savo and Kovacević, Masa and Savić, Danijela Z and Spasojević, Ivan",
year = "2014",
abstract = "An altered metal and electrolyte profile has been implicated in the pathologic mechanisms of chronic epilepsy; however, no study has comprehensively measured hippocampal concentrations of these elements in patients with mesial temporal lobe epilepsy and hippocampal sclerosis (mTLE-HS). We therefore analyzed hippocampi of 24 patients with drug-resistant mTLE-HS (mean age 35.69.4years) who underwent anterior temporal lobe resection and amygdalohippocampectomy and 17 hippocampi obtained by autopsy from 13 controls (mean age 40.5 +/- 12.9years), using inductively coupled plasma optical emission spectrometry (ICP-OES). Epileptic hippocampi showed significantly lower concentrations (g/g of tissue) of copper (HS: 2.34 +/- 0.12; control [C]: 3.57 +/- 0.33; p lt 0.001), manganese (HS: 0.205 +/- 0.030; C: 0.409 +/- 0.064; p=0.004), and potassium (HS: 2,001 +/- 59; C: 2,322 +/- 61; p lt 0.001), and increased sodium levels (HS: 1,131 +/- 22; C: 1,040 +/- 25; p=0.010). Zinc, iron, calcium, and magnesium levels did not differ in HS and controls. In summary, copper and manganese levels are deficient, whereas iron level is unchanged in hippocampi from patients with mTLE-HS. Our results provide a basis for understanding the potential involvement of different metals and electrolytes in the pathology of HS. A PowerPoint slide summarizing this article is available for download in the Supporting Information section .",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Epilepsia",
title = "Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy",
pages = "E37-E34",
number = "5",
volume = "55",
doi = "10.1111/epi.12593"
}

Ristic, A. J., Sokic, D., Baščarević, V., Spasic, S. D., Vojvodic, N. M., Savić, S., Raicević, S., Kovacević, M., Savić, D. Z.,& Spasojević, I.. (2014). Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy. in Epilepsia
Wiley-Blackwell, Hoboken., 55(5), E34-E37.
https://doi.org/10.1111/epi.12593

Ristic AJ, Sokic D, Baščarević V, Spasic SD, Vojvodic NM, Savić S, Raicević S, Kovacević M, Savić DZ, Spasojević I. Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy. in Epilepsia. 2014;55(5):E34-E37.
doi:10.1111/epi.12593 .

Ristic, Aleksandar J, Sokic, Dragoslav, Baščarević, Vladimir, Spasic, Snežana D, Vojvodic, Nikola M, Savić, Slobodan, Raicević, Savo, Kovacević, Masa, Savić, Danijela Z, Spasojević, Ivan, "Metals and electrolytes in sclerotic hippocampi in patients with drug-resistant mesial temporal lobe epilepsy" in Epilepsia, 55, no. 5 (2014):E34-E37,
https://doi.org/10.1111/epi.12593 . .

TY  - JOUR
AU  - Bajčetić, Milica
AU  - Spasic, Snežana
AU  - Spasojević, Ivan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/818
AB  - Neonatal sepsis is one of the most fulminating conditions in neonatal intensive care units. Antipathogen and supportive care are administered routinely, but do not deliver satisfactory results. In addition, the efforts to treat neonatal sepsis with anti-inflammatory agents have generally shown to be futile. The accumulating data imply that intracellular redox changes intertwined into neonatal sepsis redox cycle represent the main cause of dysfunction of mitochondria and cells in neonatal sepsis. Our aim here is to support the new philosophy in neonatal sepsis treatment, which involves the integration of mechanisms that are responsible for cellular dysfunction and organ failure, the recognition of the most important targets, and the selection of safe agents that can stop the neonatal sepsis redox cycle by hitting the hot spots. Redox-active agents that could be beneficial for neonatal sepsis treatment according to these criteria include lactoferrin, interleukin 10, zinc and selenium supplements, ibuprofen, edaravone, and pentoxifylline.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Shock
T1  - Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents
EP  - 184
IS  - 3
SP  - 179
VL  - 42
DO  - 10.1097/SHK.0000000000000198
ER  - 

@article{
author = "Bajčetić, Milica and Spasic, Snežana and Spasojević, Ivan",
year = "2014",
abstract = "Neonatal sepsis is one of the most fulminating conditions in neonatal intensive care units. Antipathogen and supportive care are administered routinely, but do not deliver satisfactory results. In addition, the efforts to treat neonatal sepsis with anti-inflammatory agents have generally shown to be futile. The accumulating data imply that intracellular redox changes intertwined into neonatal sepsis redox cycle represent the main cause of dysfunction of mitochondria and cells in neonatal sepsis. Our aim here is to support the new philosophy in neonatal sepsis treatment, which involves the integration of mechanisms that are responsible for cellular dysfunction and organ failure, the recognition of the most important targets, and the selection of safe agents that can stop the neonatal sepsis redox cycle by hitting the hot spots. Redox-active agents that could be beneficial for neonatal sepsis treatment according to these criteria include lactoferrin, interleukin 10, zinc and selenium supplements, ibuprofen, edaravone, and pentoxifylline.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Shock",
title = "Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents",
pages = "184-179",
number = "3",
volume = "42",
doi = "10.1097/SHK.0000000000000198"
}

Bajčetić, M., Spasic, S.,& Spasojević, I.. (2014). Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents. in Shock
Lippincott Williams & Wilkins, Philadelphia., 42(3), 179-184.
https://doi.org/10.1097/SHK.0000000000000198

Bajčetić M, Spasic S, Spasojević I. Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents. in Shock. 2014;42(3):179-184.
doi:10.1097/SHK.0000000000000198 .

Bajčetić, Milica, Spasic, Snežana, Spasojević, Ivan, "Redox therapy in neonatal sepsis: reasons, targets, strategy, and agents" in Shock, 42, no. 3 (2014):179-184,
https://doi.org/10.1097/SHK.0000000000000198 . .

TY  - JOUR
AU  - Bajčetić, Milica
AU  - Otasević, Biljana
AU  - Bozinovic-Prekajski, Niveska
AU  - Spasic, Snežana
AU  - Spasojević, Ivan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/817
AB  - Background: Vitamin E is routinely supplemented to preterm babies, including those with neonatal sepsis. Our aim was to examine the effects of neonatal sepsis and vitamin E on antioxidative system (AOS) in the blood. Methods: A prospective, randomized, open label study involved 65 preterm neonates (control/sepsis - 34/31), which were divided into two subgroups - non-supplemented and supplemented with vitamin E (25 IU/day for 60 days). The activities of superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were determined in erythrocytes at days 0, 30, and 60, following sepsis diagnosis. Results: There was no difference in the activity of AOS between controls and neonates with ongoing sepsis. At 60 days, septic neonates showed higher CAT activity compared to controls (P = 0.027), and lower GPx activity compared to 0 days (P = 0.022). The later was mitigated by vitamin E, which on the other hand provoked lower GPx activity at 30 days, compared to untreated septic neonates (P = 0.014). In addition, vitamin E suppressed GR activity in septic neonates (P = 0.025 and P = 0.017 at 30 and 60 days). Finally, vitamin E supplementation in control neonates provoked a significant increase of GPx activity (P = 0.015 at 60 days). Conclusions: The absence of altered redox settings in the blood of neonates during sepsis episode, and vitamin E-provoked decrease in the activity of some components of AOS, suggest that the supplementation of vitamin E in these patients might not be rational.
PB  - Sage Publications Inc, Thousand Oaks
T2  - Annals of Clinical Biochemistry
T1  - Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation
EP  - 556
IS  - 5
SP  - 550
VL  - 51
DO  - 10.1177/0004563213503317
ER  - 

@article{
author = "Bajčetić, Milica and Otasević, Biljana and Bozinovic-Prekajski, Niveska and Spasic, Snežana and Spasojević, Ivan",
year = "2014",
abstract = "Background: Vitamin E is routinely supplemented to preterm babies, including those with neonatal sepsis. Our aim was to examine the effects of neonatal sepsis and vitamin E on antioxidative system (AOS) in the blood. Methods: A prospective, randomized, open label study involved 65 preterm neonates (control/sepsis - 34/31), which were divided into two subgroups - non-supplemented and supplemented with vitamin E (25 IU/day for 60 days). The activities of superoxide dismutase, catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were determined in erythrocytes at days 0, 30, and 60, following sepsis diagnosis. Results: There was no difference in the activity of AOS between controls and neonates with ongoing sepsis. At 60 days, septic neonates showed higher CAT activity compared to controls (P = 0.027), and lower GPx activity compared to 0 days (P = 0.022). The later was mitigated by vitamin E, which on the other hand provoked lower GPx activity at 30 days, compared to untreated septic neonates (P = 0.014). In addition, vitamin E suppressed GR activity in septic neonates (P = 0.025 and P = 0.017 at 30 and 60 days). Finally, vitamin E supplementation in control neonates provoked a significant increase of GPx activity (P = 0.015 at 60 days). Conclusions: The absence of altered redox settings in the blood of neonates during sepsis episode, and vitamin E-provoked decrease in the activity of some components of AOS, suggest that the supplementation of vitamin E in these patients might not be rational.",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Annals of Clinical Biochemistry",
title = "Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation",
pages = "556-550",
number = "5",
volume = "51",
doi = "10.1177/0004563213503317"
}

Bajčetić, M., Otasević, B., Bozinovic-Prekajski, N., Spasic, S.,& Spasojević, I.. (2014). Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation. in Annals of Clinical Biochemistry
Sage Publications Inc, Thousand Oaks., 51(5), 550-556.
https://doi.org/10.1177/0004563213503317

Bajčetić M, Otasević B, Bozinovic-Prekajski N, Spasic S, Spasojević I. Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation. in Annals of Clinical Biochemistry. 2014;51(5):550-556.
doi:10.1177/0004563213503317 .

Bajčetić, Milica, Otasević, Biljana, Bozinovic-Prekajski, Niveska, Spasic, Snežana, Spasojević, Ivan, "Antioxidative system in the erythrocytes of preterm neonates with sepsis: the effects of vitamin E supplementation" in Annals of Clinical Biochemistry, 51, no. 5 (2014):550-556,
https://doi.org/10.1177/0004563213503317 . .

TY  - JOUR
AU  - Mijusković, Ana
AU  - Oreščanin-Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavić, Marija
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
AU  - Blagojević, Duško P
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/787
AB  - Background: Our aim was to investigate the effect of methanethiol (CH3SH) on contractility of rat uterus and activities of redox-active enzymes, and to compare them with the effect of sodium sulphide (Na2S), a hydrogen sulphide (H2S/HS-) donor. Methods: Uteri were isolated from virgin Wistar rats, divided into six groups, controls (untreated uteri allowed to contract spontaneously and in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced). Underlying antioxidative enzyme activities (superoxide dismutase - SOD, glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or Na2S-treated uteri were compared to controls. Results: Our experiments showed that CH3SH and Na2S provoked reversible relaxation of both spontaneous and Ca2+ induced uterine contractions. The dose-response curves differed in shape, and CH3SH curve was shifted to higher concentration compared to H2S/HS-. The effects of Na2S fitted sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked increased SOD activity and decreased GR activity. However, Na2S (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+ stimulated uteri, while the activity of GSHPx was increased in both types of active uteri. Conclusion: Our results imply that CH3SH may have a constructive role in the control of muscle function and metabolism. Observed differences between CH3SH and H2S/HS- could be attributed to a larger moiety that is present in CH3SH compared to H2S, but they are more likely to be a consequence of the specific actions of HS-, in relation to its negative charge.
PB  - Polish Acad Sciences Inst Pharmacology, Krakow
T2  - Pharmacological Reports
T1  - Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity
EP  - 379
IS  - 3
SP  - 373
VL  - 66
DO  - 10.1016/j.pharep.2013.12.012
ER  - 

@article{
author = "Mijusković, Ana and Oreščanin-Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavić, Marija and Spasić, Mihajlo and Spasojević, Ivan and Blagojević, Duško P",
year = "2014",
abstract = "Background: Our aim was to investigate the effect of methanethiol (CH3SH) on contractility of rat uterus and activities of redox-active enzymes, and to compare them with the effect of sodium sulphide (Na2S), a hydrogen sulphide (H2S/HS-) donor. Methods: Uteri were isolated from virgin Wistar rats, divided into six groups, controls (untreated uteri allowed to contract spontaneously and in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced). Underlying antioxidative enzyme activities (superoxide dismutase - SOD, glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or Na2S-treated uteri were compared to controls. Results: Our experiments showed that CH3SH and Na2S provoked reversible relaxation of both spontaneous and Ca2+ induced uterine contractions. The dose-response curves differed in shape, and CH3SH curve was shifted to higher concentration compared to H2S/HS-. The effects of Na2S fitted sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked increased SOD activity and decreased GR activity. However, Na2S (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+ stimulated uteri, while the activity of GSHPx was increased in both types of active uteri. Conclusion: Our results imply that CH3SH may have a constructive role in the control of muscle function and metabolism. Observed differences between CH3SH and H2S/HS- could be attributed to a larger moiety that is present in CH3SH compared to H2S, but they are more likely to be a consequence of the specific actions of HS-, in relation to its negative charge.",
publisher = "Polish Acad Sciences Inst Pharmacology, Krakow",
journal = "Pharmacological Reports",
title = "Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity",
pages = "379-373",
number = "3",
volume = "66",
doi = "10.1016/j.pharep.2013.12.012"
}

Mijusković, A., Oreščanin-Dušić, Z., Nikolić-Kokić, A., Slavić, M., Spasić, M., Spasojević, I.,& Blagojević, D. P.. (2014). Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity. in Pharmacological Reports
Polish Acad Sciences Inst Pharmacology, Krakow., 66(3), 373-379.
https://doi.org/10.1016/j.pharep.2013.12.012

Mijusković A, Oreščanin-Dušić Z, Nikolić-Kokić A, Slavić M, Spasić M, Spasojević I, Blagojević DP. Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity. in Pharmacological Reports. 2014;66(3):373-379.
doi:10.1016/j.pharep.2013.12.012 .

Mijusković, Ana, Oreščanin-Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavić, Marija, Spasić, Mihajlo, Spasojević, Ivan, Blagojević, Duško P, "Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity" in Pharmacological Reports, 66, no. 3 (2014):373-379,
https://doi.org/10.1016/j.pharep.2013.12.012 . .

TY  - JOUR
AU  - Ajdzanović, Vladimir Z
AU  - Mojic, Marija
AU  - Maksimović-Ivanić, Danijela
AU  - Bulatović, Mirna Z
AU  - Mijatović, Sanja
AU  - Milošević, Verica Lj.
AU  - Spasojević, Ivan
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/729
AB  - Soy isoflavones represent hopeful unconventional remedies in the therapy of prostate cancer. The aim of our study was to determine the effects of genistein and daidzein on the parameters that reflect metastatic potential, membrane fluidity, invasiveness and dynamic phenotype in Matrigel of LNCaP and PC-3 prostate cancer cells. Cell viability tests, using a wide range of concentrations of soy isoflavones (6-75 mu g/ml for 72 h), were conducted to determine their IC50 concentrations. Electron paramagnetic resonance investigations of prostate cancer cell membrane fluidity were performed at IC50 concentrations of genistein and daidzein (12.5 and 25 mu g/ml, respectively, for 10 min). Genistein provoked significant increases in the membrane order parameter (which is reciprocally proportional to membrane fluidity) of 0.722 +/- A 0.006 (LNCaP), 0.753 +/- A 0.010 (LNCaP + genistein), 0.723 +/- A 0.007 (PC-3) and 0.741 +/- A 0.004 (PC-3 + genistein); however, no such effects were observed for daidzein. While both genistein and daidzein reduced the proliferation of prostate cancer cells at their respective IC50 concentrations, during the 72 h of incubation only genistein provoked effects on the dynamic phenotype and decreased invasiveness. The effect was more evident in PC-3 cells compared to LNCaP cells. Our results imply that (1) invasive activity is at least partially dependent on membrane fluidity, (2) genistein may exert its antimetastatic effects by changing the mechanical properties of prostate cancer cells and (3) daidzein should be applied at higher concentrations than genistein in order to achieve pharmacological effects.
PB  - Springer, New York
T2  - Journal of Membrane Biology
T1  - Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones
EP  - 314
IS  - 4
SP  - 307
VL  - 246
DO  - 10.1007/s00232-013-9531-1
ER  - 

@article{
author = "Ajdzanović, Vladimir Z and Mojic, Marija and Maksimović-Ivanić, Danijela and Bulatović, Mirna Z and Mijatović, Sanja and Milošević, Verica Lj. and Spasojević, Ivan",
year = "2013",
abstract = "Soy isoflavones represent hopeful unconventional remedies in the therapy of prostate cancer. The aim of our study was to determine the effects of genistein and daidzein on the parameters that reflect metastatic potential, membrane fluidity, invasiveness and dynamic phenotype in Matrigel of LNCaP and PC-3 prostate cancer cells. Cell viability tests, using a wide range of concentrations of soy isoflavones (6-75 mu g/ml for 72 h), were conducted to determine their IC50 concentrations. Electron paramagnetic resonance investigations of prostate cancer cell membrane fluidity were performed at IC50 concentrations of genistein and daidzein (12.5 and 25 mu g/ml, respectively, for 10 min). Genistein provoked significant increases in the membrane order parameter (which is reciprocally proportional to membrane fluidity) of 0.722 +/- A 0.006 (LNCaP), 0.753 +/- A 0.010 (LNCaP + genistein), 0.723 +/- A 0.007 (PC-3) and 0.741 +/- A 0.004 (PC-3 + genistein); however, no such effects were observed for daidzein. While both genistein and daidzein reduced the proliferation of prostate cancer cells at their respective IC50 concentrations, during the 72 h of incubation only genistein provoked effects on the dynamic phenotype and decreased invasiveness. The effect was more evident in PC-3 cells compared to LNCaP cells. Our results imply that (1) invasive activity is at least partially dependent on membrane fluidity, (2) genistein may exert its antimetastatic effects by changing the mechanical properties of prostate cancer cells and (3) daidzein should be applied at higher concentrations than genistein in order to achieve pharmacological effects.",
publisher = "Springer, New York",
journal = "Journal of Membrane Biology",
title = "Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones",
pages = "314-307",
number = "4",
volume = "246",
doi = "10.1007/s00232-013-9531-1"
}

Ajdzanović, V. Z., Mojic, M., Maksimović-Ivanić, D., Bulatović, M. Z., Mijatović, S., Milošević, V. Lj.,& Spasojević, I.. (2013). Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones. in Journal of Membrane Biology
Springer, New York., 246(4), 307-314.
https://doi.org/10.1007/s00232-013-9531-1

Ajdzanović VZ, Mojic M, Maksimović-Ivanić D, Bulatović MZ, Mijatović S, Milošević VL, Spasojević I. Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones. in Journal of Membrane Biology. 2013;246(4):307-314.
doi:10.1007/s00232-013-9531-1 .

Ajdzanović, Vladimir Z, Mojic, Marija, Maksimović-Ivanić, Danijela, Bulatović, Mirna Z, Mijatović, Sanja, Milošević, Verica Lj., Spasojević, Ivan, "Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones" in Journal of Membrane Biology, 246, no. 4 (2013):307-314,
https://doi.org/10.1007/s00232-013-9531-1 . .

TY  - JOUR
AU  - Miljković, Đorđe
AU  - Spasojević, Ivan
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/731
AB  - The pathophysiology of multiple sclerosis (MS) involves several components: redox, inflammatory/autoimmune, vascular, and neurodegenerative. All of them are supported by the intertwined lines of evidence, and none of them should be written off. However, the exact mechanisms of MS initiation, its development, and progression are still elusive, despite the impressive pace by which the data on MS are accumulating. In this review, we will try to integrate the current facts and concepts, focusing on the role of redox changes and various reactive species in MS. Knowing the schedule of initial changes in pathogenic factors and the key turning points, as well as understanding the redox processes involved in MS pathogenesis is the way to enable MS prevention, early treatment, and the development of therapies that target specific pathophysiological components of the heterogeneous mechanisms of MS, which could alleviate the symptoms and hopefully stop MS. Pertinent to this, we will outline (i) redox processes involved in MS initiation; (ii) the role of reactive species in inflammation; (iii) prooxidative changes responsible for neurodegeneration; and (iv) the potential of antioxidative therapy. Antioxid. Redox Signal. 19, 2286-2334.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Antioxidants & Redox Signaling
T1  - Multiple Sclerosis: Molecular Mechanisms and Therapeutic Opportunities
EP  - 2334
IS  - 18
SP  - 2286
VL  - 19
DO  - 10.1089/ars.2012.5068
ER  - 

@article{
author = "Miljković, Đorđe and Spasojević, Ivan",
year = "2013",
abstract = "The pathophysiology of multiple sclerosis (MS) involves several components: redox, inflammatory/autoimmune, vascular, and neurodegenerative. All of them are supported by the intertwined lines of evidence, and none of them should be written off. However, the exact mechanisms of MS initiation, its development, and progression are still elusive, despite the impressive pace by which the data on MS are accumulating. In this review, we will try to integrate the current facts and concepts, focusing on the role of redox changes and various reactive species in MS. Knowing the schedule of initial changes in pathogenic factors and the key turning points, as well as understanding the redox processes involved in MS pathogenesis is the way to enable MS prevention, early treatment, and the development of therapies that target specific pathophysiological components of the heterogeneous mechanisms of MS, which could alleviate the symptoms and hopefully stop MS. Pertinent to this, we will outline (i) redox processes involved in MS initiation; (ii) the role of reactive species in inflammation; (iii) prooxidative changes responsible for neurodegeneration; and (iv) the potential of antioxidative therapy. Antioxid. Redox Signal. 19, 2286-2334.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Antioxidants & Redox Signaling",
title = "Multiple Sclerosis: Molecular Mechanisms and Therapeutic Opportunities",
pages = "2334-2286",
number = "18",
volume = "19",
doi = "10.1089/ars.2012.5068"
}

Miljković, Đ.,& Spasojević, I.. (2013). Multiple Sclerosis: Molecular Mechanisms and Therapeutic Opportunities. in Antioxidants & Redox Signaling
Mary Ann Liebert, Inc, New Rochelle., 19(18), 2286-2334.
https://doi.org/10.1089/ars.2012.5068

Miljković Đ, Spasojević I. Multiple Sclerosis: Molecular Mechanisms and Therapeutic Opportunities. in Antioxidants & Redox Signaling. 2013;19(18):2286-2334.
doi:10.1089/ars.2012.5068 .

Miljković, Đorđe, Spasojević, Ivan, "Multiple Sclerosis: Molecular Mechanisms and Therapeutic Opportunities" in Antioxidants & Redox Signaling, 19, no. 18 (2013):2286-2334,
https://doi.org/10.1089/ars.2012.5068 . .

TY  - JOUR
AU  - Sonmez, Melda
AU  - Ince, Huseyin Yavuz
AU  - Yalcin, Ozlem
AU  - Ajdzanović, Vladimir Z
AU  - Spasojević, Ivan
AU  - Meiselman, Herbert J
AU  - Baskurt, Oguz K
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/726
AB  - The role of membrane fluidity in determining red blood cell (RBC) deformability has been suggested by a number of studies. The present investigation evaluated alterations of RBC membrane fluidity, deformability and stability in the presence of four linear alcohols (methanol, ethanol, propanol and butanol) using ektacytometry and electron paramagnetic resonance (EPR) spectroscopy. All alcohols had a biphasic effect on deformability such that it increased then decreased with increasing concentration; the critical concentration for reversal was an inverse function of molecular size. EPR results showed biphasic changes of near-surface fluidity (i.e., increase then decrease) and a decreased fluidity of the lipid core; rank order of effectiveness was butanol > propanol > ethanol > methanol, with a significant correlation between near-surface fluidity and deformability (r = 0.697; p lt 0.01). The presence of alcohol enhanced the impairment of RBC deformability caused by subjecting cells to 100 Pa shear stress for 300 s, with significant differences from control being observed at higher concentrations of all four alcohols. The level of hemolysis was dependent on molecular size and concentration, whereas echinocytic shape transformation (i.e., biconcave disc to crenated morphology) was observed only for ethanol and propanol. These results are in accordance with available data obtained on model membranes. They document the presence of mechanical links between RBC deformability and near-surface membrane fluidity, chain length-dependence of the ability of alcohols to alter RBC mechanical behavior, and the biphasic response of RBC deformability and near-surface membrane fluidity to increasing alcohol concentrations.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size
IS  - 9
VL  - 8
DO  - 10.1371/journal.pone.0076579
ER  - 

@article{
author = "Sonmez, Melda and Ince, Huseyin Yavuz and Yalcin, Ozlem and Ajdzanović, Vladimir Z and Spasojević, Ivan and Meiselman, Herbert J and Baskurt, Oguz K",
year = "2013",
abstract = "The role of membrane fluidity in determining red blood cell (RBC) deformability has been suggested by a number of studies. The present investigation evaluated alterations of RBC membrane fluidity, deformability and stability in the presence of four linear alcohols (methanol, ethanol, propanol and butanol) using ektacytometry and electron paramagnetic resonance (EPR) spectroscopy. All alcohols had a biphasic effect on deformability such that it increased then decreased with increasing concentration; the critical concentration for reversal was an inverse function of molecular size. EPR results showed biphasic changes of near-surface fluidity (i.e., increase then decrease) and a decreased fluidity of the lipid core; rank order of effectiveness was butanol > propanol > ethanol > methanol, with a significant correlation between near-surface fluidity and deformability (r = 0.697; p lt 0.01). The presence of alcohol enhanced the impairment of RBC deformability caused by subjecting cells to 100 Pa shear stress for 300 s, with significant differences from control being observed at higher concentrations of all four alcohols. The level of hemolysis was dependent on molecular size and concentration, whereas echinocytic shape transformation (i.e., biconcave disc to crenated morphology) was observed only for ethanol and propanol. These results are in accordance with available data obtained on model membranes. They document the presence of mechanical links between RBC deformability and near-surface membrane fluidity, chain length-dependence of the ability of alcohols to alter RBC mechanical behavior, and the biphasic response of RBC deformability and near-surface membrane fluidity to increasing alcohol concentrations.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size",
number = "9",
volume = "8",
doi = "10.1371/journal.pone.0076579"
}

Sonmez, M., Ince, H. Y., Yalcin, O., Ajdzanović, V. Z., Spasojević, I., Meiselman, H. J.,& Baskurt, O. K.. (2013). The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size. in PLoS One
Public Library Science, San Francisco., 8(9).
https://doi.org/10.1371/journal.pone.0076579

Sonmez M, Ince HY, Yalcin O, Ajdzanović VZ, Spasojević I, Meiselman HJ, Baskurt OK. The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size. in PLoS One. 2013;8(9).
doi:10.1371/journal.pone.0076579 .

Sonmez, Melda, Ince, Huseyin Yavuz, Yalcin, Ozlem, Ajdzanović, Vladimir Z, Spasojević, Ivan, Meiselman, Herbert J, Baskurt, Oguz K, "The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size" in PLoS One, 8, no. 9 (2013),
https://doi.org/10.1371/journal.pone.0076579 . .