@article{
author = "Žižić, Milan and Stanić, Marina and AQUILANTI, Giuliana and Bajuk-Bogdanovic, Danica and Branković, Goran and Rodić, Ivanka and Živić, Miroslav and Zakrzewska, Joanna",
year = "2022",
abstract = "Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms
and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation.
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as
well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red
coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of
SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter
of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium
revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile
dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time.
Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer
chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy",
publisher = "Springer-Verlag GmbH Berlin Heidelberg",
journal = "Analytical and Bianalytical Chemistry",
title = "Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus",
pages = "6222.-6213",
number = "20",
volume = "414",
doi = "10.1007/s00216-022-04191-4"
}