TY  - JOUR
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Milić Komić, Sonja
AU  - Zechmann, Bernd
AU  - Albert, Andreas
AU  - Winkler, Jana Barbro
AU  - Veljović-Jovanović, Sonja
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/873
AB  - We used variegated Plectranthus coleoides as a model plant with the aim of clarifying whether the effects of realistic ultraviolet-B (UV-B) doses on phenolic metabolism in leaves are mediated by photosynthesis. Plants were exposed to UV-B radiation (0.90Wm(-2)) combined with two photosynthetically active radiation (PAR) intensities [395 and 1350molm(-2)s(-1), low light (LL) and high light (HL)] for 9d in sun simulators. Our study indicates that UV-B component of sunlight stimulates CO2 assimilation and stomatal conductance, depending on background light. UV-B-specific induction of apigenin and cyanidin glycosides was observed in both green and white tissues. However, all the other phenolic subclasses were up to four times more abundant in green leaf tissue. Caffeic and rosmarinic acids, catechin and epicatechin, which are endogenous peroxidase substrates, were depleted at HL in green tissue. This was correlated with increased peroxidase and ascorbate peroxidase activities and increased ascorbate content. The UV-B supplement to HL attenuated antioxidative metabolism and partly recovered the phenolic pool indicating stimulation of the phenylpropanoid pathway. In summary, we propose that ortho-dihydroxy phenolics are involved in antioxidative defence in chlorophyllous tissue upon light excess, while apigenin and cyanidin in white tissue have preferentially UV-screening function.
PB  - Wiley, Hoboken
T2  - Plant Cell and Environment
T1  - Ultraviolet-B component of sunlight stimulates photosynthesis and flavonoid accumulation in variegated Plectranthus coleoides leaves depending on background light
EP  - 979
IS  - 5
SP  - 968
VL  - 38
DO  - 10.1111/pce.12471
ER  - 

@article{
author = "Vidović, Marija and Morina, Filis and Milić Komić, Sonja and Zechmann, Bernd and Albert, Andreas and Winkler, Jana Barbro and Veljović-Jovanović, Sonja",
year = "2015",
abstract = "We used variegated Plectranthus coleoides as a model plant with the aim of clarifying whether the effects of realistic ultraviolet-B (UV-B) doses on phenolic metabolism in leaves are mediated by photosynthesis. Plants were exposed to UV-B radiation (0.90Wm(-2)) combined with two photosynthetically active radiation (PAR) intensities [395 and 1350molm(-2)s(-1), low light (LL) and high light (HL)] for 9d in sun simulators. Our study indicates that UV-B component of sunlight stimulates CO2 assimilation and stomatal conductance, depending on background light. UV-B-specific induction of apigenin and cyanidin glycosides was observed in both green and white tissues. However, all the other phenolic subclasses were up to four times more abundant in green leaf tissue. Caffeic and rosmarinic acids, catechin and epicatechin, which are endogenous peroxidase substrates, were depleted at HL in green tissue. This was correlated with increased peroxidase and ascorbate peroxidase activities and increased ascorbate content. The UV-B supplement to HL attenuated antioxidative metabolism and partly recovered the phenolic pool indicating stimulation of the phenylpropanoid pathway. In summary, we propose that ortho-dihydroxy phenolics are involved in antioxidative defence in chlorophyllous tissue upon light excess, while apigenin and cyanidin in white tissue have preferentially UV-screening function.",
publisher = "Wiley, Hoboken",
journal = "Plant Cell and Environment",
title = "Ultraviolet-B component of sunlight stimulates photosynthesis and flavonoid accumulation in variegated Plectranthus coleoides leaves depending on background light",
pages = "979-968",
number = "5",
volume = "38",
doi = "10.1111/pce.12471"
}

Vidović, M., Morina, F., Milić Komić, S., Zechmann, B., Albert, A., Winkler, J. B.,& Veljović-Jovanović, S.. (2015). Ultraviolet-B component of sunlight stimulates photosynthesis and flavonoid accumulation in variegated Plectranthus coleoides leaves depending on background light. in Plant Cell and Environment
Wiley, Hoboken., 38(5), 968-979.
https://doi.org/10.1111/pce.12471

Vidović M, Morina F, Milić Komić S, Zechmann B, Albert A, Winkler JB, Veljović-Jovanović S. Ultraviolet-B component of sunlight stimulates photosynthesis and flavonoid accumulation in variegated Plectranthus coleoides leaves depending on background light. in Plant Cell and Environment. 2015;38(5):968-979.
doi:10.1111/pce.12471 .

Vidović, Marija, Morina, Filis, Milić Komić, Sonja, Zechmann, Bernd, Albert, Andreas, Winkler, Jana Barbro, Veljović-Jovanović, Sonja, "Ultraviolet-B component of sunlight stimulates photosynthesis and flavonoid accumulation in variegated Plectranthus coleoides leaves depending on background light" in Plant Cell and Environment, 38, no. 5 (2015):968-979,
https://doi.org/10.1111/pce.12471 . .

TY  - JOUR
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Milić Komić, Sonja
AU  - Albert, Andreas
AU  - Zechmann, Bernd
AU  - Tosti, Tomislav
AU  - Winkler, Jana Barbro
AU  - Veljović-Jovanović, Sonja
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/858
AB  - We studied the specific effects of high photosynthetically active radiation (PAR, 400-700 nm) and ecologically relevant UV-B radiation (0.90 W m(-2)) on antioxidative and phenolic metabolism by exploiting the green-white leaf variegation of Pelargonium zonale plants. This is a suitable model system for examining "source-sink" interactions within the same leaf. High PAR intensity (1350 mu mol m(-2) s(-1)) and UV-B radiation induced different responses in green and white leaf sectors. High PAR intensity had a greater influence on green tissue, triggering the accumulation of phenylpropanoids and flavonoids with strong antioxidative function. Induced phenolics, together with ascorbate, ascorbate peroxidase (APX, EC 1.11.1.11) and catalase (CAT, EC 1.11.1.6) provided efficient defense against potential oxidative pressure. UV-B-induced up-regulation of non-phenolic H2O2 scavengers in green leaf sectors was greater than high PAR-induced changes, indicating a UV-B role in antioxidative defense under light excess; on the contrary, minimal effects were observed in white tissue. However, UV-B radiation had greater influence on phenolics in white leaf sections compared to green ones, inducing accumulation of phenolic glycosides whose function was UV-B screening rather than antioxidative. By stimulation of starch and sucrose breakdown and carbon allocation in the form of soluble sugars from "source" (green) tissue to "sink" (white) tissue, UV-B radiation compensated the absence of photosynthetic activity and phenylpropanoid and flavonoid biosynthesis in white sectors.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Carbon allocation from source to sink leaf tissue in relation to flavonoid biosynthesis in variegated Pelargonium zonale under UV-B radiation and high PAR intensity
EP  - 55
SP  - 44
VL  - 93
DO  - 10.1016/j.plaphy.2015.01.008
ER  - 

@article{
author = "Vidović, Marija and Morina, Filis and Milić Komić, Sonja and Albert, Andreas and Zechmann, Bernd and Tosti, Tomislav and Winkler, Jana Barbro and Veljović-Jovanović, Sonja",
year = "2015",
abstract = "We studied the specific effects of high photosynthetically active radiation (PAR, 400-700 nm) and ecologically relevant UV-B radiation (0.90 W m(-2)) on antioxidative and phenolic metabolism by exploiting the green-white leaf variegation of Pelargonium zonale plants. This is a suitable model system for examining "source-sink" interactions within the same leaf. High PAR intensity (1350 mu mol m(-2) s(-1)) and UV-B radiation induced different responses in green and white leaf sectors. High PAR intensity had a greater influence on green tissue, triggering the accumulation of phenylpropanoids and flavonoids with strong antioxidative function. Induced phenolics, together with ascorbate, ascorbate peroxidase (APX, EC 1.11.1.11) and catalase (CAT, EC 1.11.1.6) provided efficient defense against potential oxidative pressure. UV-B-induced up-regulation of non-phenolic H2O2 scavengers in green leaf sectors was greater than high PAR-induced changes, indicating a UV-B role in antioxidative defense under light excess; on the contrary, minimal effects were observed in white tissue. However, UV-B radiation had greater influence on phenolics in white leaf sections compared to green ones, inducing accumulation of phenolic glycosides whose function was UV-B screening rather than antioxidative. By stimulation of starch and sucrose breakdown and carbon allocation in the form of soluble sugars from "source" (green) tissue to "sink" (white) tissue, UV-B radiation compensated the absence of photosynthetic activity and phenylpropanoid and flavonoid biosynthesis in white sectors.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Carbon allocation from source to sink leaf tissue in relation to flavonoid biosynthesis in variegated Pelargonium zonale under UV-B radiation and high PAR intensity",
pages = "55-44",
volume = "93",
doi = "10.1016/j.plaphy.2015.01.008"
}

Vidović, M., Morina, F., Milić Komić, S., Albert, A., Zechmann, B., Tosti, T., Winkler, J. B.,& Veljović-Jovanović, S.. (2015). Carbon allocation from source to sink leaf tissue in relation to flavonoid biosynthesis in variegated Pelargonium zonale under UV-B radiation and high PAR intensity. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 93, 44-55.
https://doi.org/10.1016/j.plaphy.2015.01.008

Vidović M, Morina F, Milić Komić S, Albert A, Zechmann B, Tosti T, Winkler JB, Veljović-Jovanović S. Carbon allocation from source to sink leaf tissue in relation to flavonoid biosynthesis in variegated Pelargonium zonale under UV-B radiation and high PAR intensity. in Plant Physiology and Biochemistry. 2015;93:44-55.
doi:10.1016/j.plaphy.2015.01.008 .

Vidović, Marija, Morina, Filis, Milić Komić, Sonja, Albert, Andreas, Zechmann, Bernd, Tosti, Tomislav, Winkler, Jana Barbro, Veljović-Jovanović, Sonja, "Carbon allocation from source to sink leaf tissue in relation to flavonoid biosynthesis in variegated Pelargonium zonale under UV-B radiation and high PAR intensity" in Plant Physiology and Biochemistry, 93 (2015):44-55,
https://doi.org/10.1016/j.plaphy.2015.01.008 . .

TY  - JOUR
AU  - Tolin, Serena
AU  - Arrigoni, Giorgio
AU  - Trentin, Anna Rita
AU  - Veljović-Jovanović, Sonja
AU  - Pivato, Micaela
AU  - Zechmann, Bernd
AU  - Masi, Antonio
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/679
AB  - The existence of a gamma-glutamyl cycle consisting of intracellular GSH synthesis, extrusion to the apoplastic space and recovery by gamma-glutamyl transferase (GGT)-assisted degradation into its constituent amino acids, has been demonstrated in plants. To address the significance of this cycle in plant cells, we performed integrated biochemical, immunocytochemical, and quantitative proteomics analyses in the Arabidopsis thaliana ggt1 knockout mutant (lacking apoplastic GGT1 isoform) and its corresponding wild-type (WT). The ggt1 knockout leaves exhibited an increased ascorbate and GSH content, increased apoplastic GSH content, and enhanced protein carbonylations in the low-molecular weight range compared to WT. The combined iTRAQ and LC-MS/MS-based quantitative proteomics approach identified 70 proteins (out of 1013 identified proteins) whose abundance was significantly different in leaves of ggt1 mutant compared to WT, with a fold change 1.5. Mining of the proteome data for GSH-associated genes showed that disruption of gamma-glutamyl cycle in ggt1 knockout-leaves was associated with the induction of genes encoding four GSTs in the phi class (GSTF2, GSTF6, GSTF9, and GSTF10), a GSH peroxidase (GPX1), and glyoxylase II. Proteins with a lower abundance compared to the WT are involved in chloroplast functions, carbohydrate/maltose metabolism, and vegetative storage protein synthesis. Present findings suggest that GGT1 plays a role in redox signaling. The disruption of the gamma-glutamyl cycle in the ggt1 mutant results in pleiotropic effects related to biotic and abiotic stress response, antioxidant metabolism, senescence, carbohydrate metabolism, and photosynthesis, with strong implications for plant adaptation to the environment.
PB  - Wiley, Hoboken
T2  - Proteomics
T1  - Biochemical and quantitative proteomics investigations in Arabidopsis ggt1 mutant leaves reveal a role for the gamma-glutamyl cycle in plant's adaptation to environment
EP  - 2045
IS  - 12-13
SP  - 2031
VL  - 13
DO  - 10.1002/pmic.201200479
ER  - 

@article{
author = "Tolin, Serena and Arrigoni, Giorgio and Trentin, Anna Rita and Veljović-Jovanović, Sonja and Pivato, Micaela and Zechmann, Bernd and Masi, Antonio",
year = "2013",
abstract = "The existence of a gamma-glutamyl cycle consisting of intracellular GSH synthesis, extrusion to the apoplastic space and recovery by gamma-glutamyl transferase (GGT)-assisted degradation into its constituent amino acids, has been demonstrated in plants. To address the significance of this cycle in plant cells, we performed integrated biochemical, immunocytochemical, and quantitative proteomics analyses in the Arabidopsis thaliana ggt1 knockout mutant (lacking apoplastic GGT1 isoform) and its corresponding wild-type (WT). The ggt1 knockout leaves exhibited an increased ascorbate and GSH content, increased apoplastic GSH content, and enhanced protein carbonylations in the low-molecular weight range compared to WT. The combined iTRAQ and LC-MS/MS-based quantitative proteomics approach identified 70 proteins (out of 1013 identified proteins) whose abundance was significantly different in leaves of ggt1 mutant compared to WT, with a fold change 1.5. Mining of the proteome data for GSH-associated genes showed that disruption of gamma-glutamyl cycle in ggt1 knockout-leaves was associated with the induction of genes encoding four GSTs in the phi class (GSTF2, GSTF6, GSTF9, and GSTF10), a GSH peroxidase (GPX1), and glyoxylase II. Proteins with a lower abundance compared to the WT are involved in chloroplast functions, carbohydrate/maltose metabolism, and vegetative storage protein synthesis. Present findings suggest that GGT1 plays a role in redox signaling. The disruption of the gamma-glutamyl cycle in the ggt1 mutant results in pleiotropic effects related to biotic and abiotic stress response, antioxidant metabolism, senescence, carbohydrate metabolism, and photosynthesis, with strong implications for plant adaptation to the environment.",
publisher = "Wiley, Hoboken",
journal = "Proteomics",
title = "Biochemical and quantitative proteomics investigations in Arabidopsis ggt1 mutant leaves reveal a role for the gamma-glutamyl cycle in plant's adaptation to environment",
pages = "2045-2031",
number = "12-13",
volume = "13",
doi = "10.1002/pmic.201200479"
}

Tolin, S., Arrigoni, G., Trentin, A. R., Veljović-Jovanović, S., Pivato, M., Zechmann, B.,& Masi, A.. (2013). Biochemical and quantitative proteomics investigations in Arabidopsis ggt1 mutant leaves reveal a role for the gamma-glutamyl cycle in plant's adaptation to environment. in Proteomics
Wiley, Hoboken., 13(12-13), 2031-2045.
https://doi.org/10.1002/pmic.201200479

Tolin S, Arrigoni G, Trentin AR, Veljović-Jovanović S, Pivato M, Zechmann B, Masi A. Biochemical and quantitative proteomics investigations in Arabidopsis ggt1 mutant leaves reveal a role for the gamma-glutamyl cycle in plant's adaptation to environment. in Proteomics. 2013;13(12-13):2031-2045.
doi:10.1002/pmic.201200479 .

Tolin, Serena, Arrigoni, Giorgio, Trentin, Anna Rita, Veljović-Jovanović, Sonja, Pivato, Micaela, Zechmann, Bernd, Masi, Antonio, "Biochemical and quantitative proteomics investigations in Arabidopsis ggt1 mutant leaves reveal a role for the gamma-glutamyl cycle in plant's adaptation to environment" in Proteomics, 13, no. 12-13 (2013):2031-2045,
https://doi.org/10.1002/pmic.201200479 . .