TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Stević, Zorica D
AU  - Spasic, Snežana D
AU  - Jones, David R
AU  - Morina, Arian
AU  - Spasić, Mihajlo
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/417
AB  - A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
PB  - Maney Publishing, Leeds
T2  - Redox Report
T1  - Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid
EP  - 35
IS  - 1
SP  - 29
VL  - 15
DO  - 10.1179/174329210X12650506623087
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Stević, Zorica D and Spasic, Snežana D and Jones, David R and Morina, Arian and Spasić, Mihajlo",
year = "2010",
abstract = "A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.",
publisher = "Maney Publishing, Leeds",
journal = "Redox Report",
title = "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid",
pages = "35-29",
number = "1",
volume = "15",
doi = "10.1179/174329210X12650506623087"
}

Spasojević, I., Mojović, M., Stević, Z. D., Spasic, S. D., Jones, D. R., Morina, A.,& Spasić, M.. (2010). Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report
Maney Publishing, Leeds., 15(1), 29-35.
https://doi.org/10.1179/174329210X12650506623087

Spasojević I, Mojović M, Stević ZD, Spasic SD, Jones DR, Morina A, Spasić M. Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report. 2010;15(1):29-35.
doi:10.1179/174329210X12650506623087 .

Spasojević, Ivan, Mojović, Miloš, Stević, Zorica D, Spasic, Snežana D, Jones, David R, Morina, Arian, Spasić, Mihajlo, "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid" in Redox Report, 15, no. 1 (2010):29-35,
https://doi.org/10.1179/174329210X12650506623087 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Stević, Zorica D
AU  - Nikolić-Kokić, Aleksandra
AU  - Jones, David R
AU  - Blagojević, Duško P
AU  - Spasić, Mihajlo
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/410
AB  - Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H2O2, which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H2O2 to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Redox Report
T1  - Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients
EP  - 86
IS  - 2
SP  - 81
VL  - 15
DO  - 10.1179/174329210X12650506623320
ER  - 

@article{
author = "Spasojević, Ivan and Stević, Zorica D and Nikolić-Kokić, Aleksandra and Jones, David R and Blagojević, Duško P and Spasić, Mihajlo",
year = "2010",
abstract = "Ferrous iron, released from iron deposits in the motor cortex and other brain regions of amyotrophic lateral sclerosis (ALS) patients, participates in the Fenton reaction in cerebrospinal fluid (CSF) alongside H2O2, which is continuously released by neuronal cells. In vivo, the production of notoriously reactive hydroxyl radicals via this reaction could lead to the progression of the disease. Herein, we have examined the effect of ascorbate and uric acid on the production of hydroxyl radicals in CSF from both sporadic ALS patients and control subjects. Electron paramagnetic resonance spectroscopy identified ascorbyl radicals in CSF from ALS patients whereas it was undetectable in control CSF. The addition of H2O2 to the CSF from ALS patients provoked further formation of ascorbyl radicals and the formation of hydroxyl radicals ex vivo. The hydroxyl addition of uric acid to CSF from ALS patients diminished the production of hydroxyl radicals. In conclusion, there are clear differences between the roles of the two examined radical scavengers in the CSF of ALS patients indicating that the use of ascorbate could have unfavourable effects in ALS patients.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Redox Report",
title = "Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients",
pages = "86-81",
number = "2",
volume = "15",
doi = "10.1179/174329210X12650506623320"
}

Spasojević, I., Stević, Z. D., Nikolić-Kokić, A., Jones, D. R., Blagojević, D. P.,& Spasić, M.. (2010). Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report
Taylor & Francis Ltd, Abingdon., 15(2), 81-86.
https://doi.org/10.1179/174329210X12650506623320

Spasojević I, Stević ZD, Nikolić-Kokić A, Jones DR, Blagojević DP, Spasić M. Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients. in Redox Report. 2010;15(2):81-86.
doi:10.1179/174329210X12650506623320 .

Spasojević, Ivan, Stević, Zorica D, Nikolić-Kokić, Aleksandra, Jones, David R, Blagojević, Duško P, Spasić, Mihajlo, "Different roles of radical scavengers - ascorbate and urate in the cerebrospinal fluid of amyotrophic lateral sclerosis patients" in Redox Report, 15, no. 2 (2010):81-86,
https://doi.org/10.1179/174329210X12650506623320 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Blagojević, Duško
AU  - Spasic, Snežana D.
AU  - Jones, David R
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/321
AB  - The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Research
T1  - Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical
EP  - 84
IS  - 1
SP  - 80
VL  - 344
DO  - 10.1016/j.carres.2008.09.025
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Blagojević, Duško and Spasic, Snežana D. and Jones, David R and Nikolić-Kokić, Aleksandra and Spasić, Mihajlo",
year = "2009",
abstract = "The hydroxyl radical ((OH)-O-center dot) has detrimental biological activity due to its very high reactivity. Our experiments were designed to determine the effects of equimolar concentrations of glucose, fructose and mannitol and three phosphorylated forms of fructose (fructose-1-phosphate (F1P): fructose-6-phosphate (F6P): and fructose-1,6-bis(phosphate) (F16BP)) on (OH)-O-center dot radical production via the Fenton reaction. EPR spectroscopy using spin-trap DEPMPO was applied to detect radical production. We found that the percentage inhibition of (OH)-O-center dot radical formation decreased in the order F16BP > F1P > F6P > fructose > mannitol = glucose. As ketoses can sequester redox-active iron thus preventing the Fenton reaction, the Haber-Weiss-like system was also employed to generate (OH)-O-center dot, so that the effect of iron sequestration could be distinguished from direct (OH)-O-center dot radical scavenging. In the latter system, the rank order of (OH)-O-center dot scavenging activity was F16BP > F1P > F6P > fructose = mannitol = glucose. Our results clearly demonstrate that intracellular phosphorylated forms of fructose have more scavenging properties than fructose or glucose, leading us to conclude that the acute administration of fructose could overcome the body's reaction to exogenous antioxidants during appropriate therapy in certain pathophysiological conditions related to oxidative stress, Such as sepsis, neurodegenerative diseases, atherosclerosis, malignancy, and some complications of pregnancy.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Research",
title = "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical",
pages = "84-80",
number = "1",
volume = "344",
doi = "10.1016/j.carres.2008.09.025"
}

Spasojević, I., Mojović, M., Blagojević, D., Spasic, S. D., Jones, D. R., Nikolić-Kokić, A.,& Spasić, M.. (2009). Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research
Elsevier Sci Ltd, Oxford., 344(1), 80-84.
https://doi.org/10.1016/j.carres.2008.09.025

Spasojević I, Mojović M, Blagojević D, Spasic SD, Jones DR, Nikolić-Kokić A, Spasić M. Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical. in Carbohydrate Research. 2009;344(1):80-84.
doi:10.1016/j.carres.2008.09.025 .

Spasojević, Ivan, Mojović, Miloš, Blagojević, Duško, Spasic, Snežana D., Jones, David R, Nikolić-Kokić, Aleksandra, Spasić, Mihajlo, "Relevance of the capacity of phosphorylated fructose to scavenge the hydroxyl radical" in Carbohydrate Research, 344, no. 1 (2009):80-84,
https://doi.org/10.1016/j.carres.2008.09.025 . .

TY  - JOUR
AU  - Bogdanović Pristov, Jelena
AU  - Spasojević, Ivan
AU  - Miković, Zeljko
AU  - Mandic, Vesna
AU  - Cerović, Nikola
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/330
AB  - Our aim was to investigate the activities of antioxidative defense enzymes in the placenta, fetal blood and amnion fluid in inherited thrombophilia. Thrombophilia was associated with nearly threefold increase of activity (p  lt  0.001) of the placental catalase (81.1 +/- 20.6 U/mg of proteins in controls and 270.0 +/- 69.9 U/mg in thrombophilic subjects), glutathione (GSH) peroxidase (C: 20.2 +/- 10.1 U/mg; T. 60.0 +/- 15.5 U/mg), and GSH reductase (C: 28.9 +/- 5.6 U/mg; T. 72.7 +/- 23.0 U/mg). The placental activities of superoxide dismutating enzymes-MnSOD and CuZnSOD, did not differ in controls and thrombophilia. Likewise, the activities of catalase and SOD in the fetal blood, and the level of ascorbyl radical which represents a marker of oxidative status of amniotic fluid, were similar in controls and thrombophilic subjects. From this we concluded that in thrombophilia, placental tissue is exposed to H2O2-mediated oxidative stress, which could be initiated by pro-thrombic conditions in maternal blood. Increased activity of placental H2O2-removing enzymes protects fetus and mother during pregnancy, but may increase the risk of postpartum thrombosis.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide
EP  - 18
IS  - 1
SP  - 14
VL  - 2
DO  - 10.4161/oxim.2.1.7705
ER  - 

@article{
author = "Bogdanović Pristov, Jelena and Spasojević, Ivan and Miković, Zeljko and Mandic, Vesna and Cerović, Nikola and Spasić, Mihajlo",
year = "2009",
abstract = "Our aim was to investigate the activities of antioxidative defense enzymes in the placenta, fetal blood and amnion fluid in inherited thrombophilia. Thrombophilia was associated with nearly threefold increase of activity (p  lt  0.001) of the placental catalase (81.1 +/- 20.6 U/mg of proteins in controls and 270.0 +/- 69.9 U/mg in thrombophilic subjects), glutathione (GSH) peroxidase (C: 20.2 +/- 10.1 U/mg; T. 60.0 +/- 15.5 U/mg), and GSH reductase (C: 28.9 +/- 5.6 U/mg; T. 72.7 +/- 23.0 U/mg). The placental activities of superoxide dismutating enzymes-MnSOD and CuZnSOD, did not differ in controls and thrombophilia. Likewise, the activities of catalase and SOD in the fetal blood, and the level of ascorbyl radical which represents a marker of oxidative status of amniotic fluid, were similar in controls and thrombophilic subjects. From this we concluded that in thrombophilia, placental tissue is exposed to H2O2-mediated oxidative stress, which could be initiated by pro-thrombic conditions in maternal blood. Increased activity of placental H2O2-removing enzymes protects fetus and mother during pregnancy, but may increase the risk of postpartum thrombosis.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide",
pages = "18-14",
number = "1",
volume = "2",
doi = "10.4161/oxim.2.1.7705"
}

Bogdanović Pristov, J., Spasojević, I., Miković, Z., Mandic, V., Cerović, N.,& Spasić, M.. (2009). Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London., 2(1), 14-18.
https://doi.org/10.4161/oxim.2.1.7705

Bogdanović Pristov J, Spasojević I, Miković Z, Mandic V, Cerović N, Spasić M. Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide. in Oxidative Medicine and Cellular Longevity. 2009;2(1):14-18.
doi:10.4161/oxim.2.1.7705 .

Bogdanović Pristov, Jelena, Spasojević, Ivan, Miković, Zeljko, Mandic, Vesna, Cerović, Nikola, Spasić, Mihajlo, "Antioxidative defense enzymes in placenta protect placenta and fetus in inherited thrombophilia from hydrogen peroxide" in Oxidative Medicine and Cellular Longevity, 2, no. 1 (2009):14-18,
https://doi.org/10.4161/oxim.2.1.7705 . .

TY  - JOUR
AU  - Kojic, Danijela
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Blagojević, Duško
AU  - Worland, M. Roger
AU  - Grubor-Lajsic, Gordana
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/328
AB  - The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H2O2), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H2O2 concentration was recorded. A possible regulatory role of H2O2 at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.
PB  - Czech Acad Sci, Inst Entomology, Ceske Budejovice
T2  - European Journal of Entomology
T1  - Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)
EP  - 454
IS  - 3
SP  - 451
VL  - 106
DO  - 10.14411/eje.2009.056
ER  - 

@article{
author = "Kojic, Danijela and Spasojević, Ivan and Mojović, Miloš and Blagojević, Duško and Worland, M. Roger and Grubor-Lajsic, Gordana and Spasić, Mihajlo",
year = "2009",
abstract = "The aim of this study was to investigate the relationship between antioxidant enzymes and reactive oxygen species production in diapausing larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Pyralidae) kept at 5 degrees C, -3 degrees C and -16 degrees C for two weeks. The amount of hydrogen peroxide (H2O2), activity of antioxidant enzymes, copper zinc superoxide dismutase (CuZnSOD), manganese superoxide dismutases (MnSOD) and catalase (CAT) in whole body homogenates, as well as the electron paramagnetic resonance (EPR) spectroscopy of this insect's whole body were analysed. A higher level of melanin radical and lower CuZnSOD and CAT activities were found in larvae kept at -3 degrees C than at 5 degrees C and -16 degrees C. At the same temperature (-3 degrees C) an elevated H2O2 concentration was recorded. A possible regulatory role of H2O2 at -3 degrees C, which is the temperature that triggers freezing tolerance, is suggested.",
publisher = "Czech Acad Sci, Inst Entomology, Ceske Budejovice",
journal = "European Journal of Entomology",
title = "Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)",
pages = "454-451",
number = "3",
volume = "106",
doi = "10.14411/eje.2009.056"
}

Kojic, D., Spasojević, I., Mojović, M., Blagojević, D., Worland, M. R., Grubor-Lajsic, G.,& Spasić, M.. (2009). Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). in European Journal of Entomology
Czech Acad Sci, Inst Entomology, Ceske Budejovice., 106(3), 451-454.
https://doi.org/10.14411/eje.2009.056

Kojic D, Spasojević I, Mojović M, Blagojević D, Worland MR, Grubor-Lajsic G, Spasić M. Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae). in European Journal of Entomology. 2009;106(3):451-454.
doi:10.14411/eje.2009.056 .

Kojic, Danijela, Spasojević, Ivan, Mojović, Miloš, Blagojević, Duško, Worland, M. Roger, Grubor-Lajsic, Gordana, Spasić, Mihajlo, "Potential role of hydrogen peroxide and melanin in the cold hardiness of Ostrinia nubilalis (Lepidoptera: Pyralidae)" in European Journal of Entomology, 106, no. 3 (2009):451-454,
https://doi.org/10.14411/eje.2009.056 . .

TY  - JOUR
AU  - Oreščanin-Dušić, Zorana
AU  - Milovanović, Slobodan
AU  - Blagojević, Duško
AU  - Nikolić-Kokić, Aleksandra
AU  - Radojicic, Ratko
AU  - Spasojević, Ivan
AU  - Spasić, Mihajlo
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/335
AB  - Protamine sulphate causes potassium ion channel-mediated relaxation of spontaneous and calcium ion-induced contractions of the isolated rat uterus. Diethyldithiocarbamate (DDC) potentiated the effect of protamine sulphate. A mechanism for DDC's action was postulated on the basis of its interactions with divalent iron ions and Cui Zn-SOD. DDC chelates divalent iron ions creating DDC-iron (Fe-DDC) complexes. Fe-DDC forms stable NO-Fe-DDC2 complexes by NO scavenging and de-nitrosylation processes, which in combination with DDC (5 mM) provoke inhibition of Cui Zn-SOD resulting in specific oxidative conditions culminating in potassium ion channel opening, membrane hyperpolarisation, inhibition of calcium ion influx and subsequent muscle relaxation. As Fe-DDC and NO-Fe-DDC2 complexes exclude divalent iron ions from participating in the hydroxy radical generating Fenton reaction, DDC can also prevent iron-related pathophysiological manifestations. Such permissive roles of DDC open the possibility for application of its pharmacological form (disulfiram) to a wider spectrum of pathophysiological conditions related to smooth muscles.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Redox Report
T1  - Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus
EP  - 54
IS  - 2
SP  - 48
VL  - 14
DO  - 10.1179/135100009X392476
ER  - 

@article{
author = "Oreščanin-Dušić, Zorana and Milovanović, Slobodan and Blagojević, Duško and Nikolić-Kokić, Aleksandra and Radojicic, Ratko and Spasojević, Ivan and Spasić, Mihajlo",
year = "2009",
abstract = "Protamine sulphate causes potassium ion channel-mediated relaxation of spontaneous and calcium ion-induced contractions of the isolated rat uterus. Diethyldithiocarbamate (DDC) potentiated the effect of protamine sulphate. A mechanism for DDC's action was postulated on the basis of its interactions with divalent iron ions and Cui Zn-SOD. DDC chelates divalent iron ions creating DDC-iron (Fe-DDC) complexes. Fe-DDC forms stable NO-Fe-DDC2 complexes by NO scavenging and de-nitrosylation processes, which in combination with DDC (5 mM) provoke inhibition of Cui Zn-SOD resulting in specific oxidative conditions culminating in potassium ion channel opening, membrane hyperpolarisation, inhibition of calcium ion influx and subsequent muscle relaxation. As Fe-DDC and NO-Fe-DDC2 complexes exclude divalent iron ions from participating in the hydroxy radical generating Fenton reaction, DDC can also prevent iron-related pathophysiological manifestations. Such permissive roles of DDC open the possibility for application of its pharmacological form (disulfiram) to a wider spectrum of pathophysiological conditions related to smooth muscles.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Redox Report",
title = "Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus",
pages = "54-48",
number = "2",
volume = "14",
doi = "10.1179/135100009X392476"
}

Oreščanin-Dušić, Z., Milovanović, S., Blagojević, D., Nikolić-Kokić, A., Radojicic, R., Spasojević, I.,& Spasić, M.. (2009). Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus. in Redox Report
Taylor & Francis Ltd, Abingdon., 14(2), 48-54.
https://doi.org/10.1179/135100009X392476

Oreščanin-Dušić Z, Milovanović S, Blagojević D, Nikolić-Kokić A, Radojicic R, Spasojević I, Spasić M. Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus. in Redox Report. 2009;14(2):48-54.
doi:10.1179/135100009X392476 .

Oreščanin-Dušić, Zorana, Milovanović, Slobodan, Blagojević, Duško, Nikolić-Kokić, Aleksandra, Radojicic, Ratko, Spasojević, Ivan, Spasić, Mihajlo, "Diethyldithiocarbamate potentiates the effects of protamine sulphate in the isolated rat uterus" in Redox Report, 14, no. 2 (2009):48-54,
https://doi.org/10.1179/135100009X392476 . .