TY  - JOUR
AU  - Ajdzanović, Vladimir Z
AU  - Mojic, Marija
AU  - Maksimović-Ivanić, Danijela
AU  - Bulatović, Mirna Z
AU  - Mijatović, Sanja
AU  - Milošević, Verica Lj.
AU  - Spasojević, Ivan
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/729
AB  - Soy isoflavones represent hopeful unconventional remedies in the therapy of prostate cancer. The aim of our study was to determine the effects of genistein and daidzein on the parameters that reflect metastatic potential, membrane fluidity, invasiveness and dynamic phenotype in Matrigel of LNCaP and PC-3 prostate cancer cells. Cell viability tests, using a wide range of concentrations of soy isoflavones (6-75 mu g/ml for 72 h), were conducted to determine their IC50 concentrations. Electron paramagnetic resonance investigations of prostate cancer cell membrane fluidity were performed at IC50 concentrations of genistein and daidzein (12.5 and 25 mu g/ml, respectively, for 10 min). Genistein provoked significant increases in the membrane order parameter (which is reciprocally proportional to membrane fluidity) of 0.722 +/- A 0.006 (LNCaP), 0.753 +/- A 0.010 (LNCaP + genistein), 0.723 +/- A 0.007 (PC-3) and 0.741 +/- A 0.004 (PC-3 + genistein); however, no such effects were observed for daidzein. While both genistein and daidzein reduced the proliferation of prostate cancer cells at their respective IC50 concentrations, during the 72 h of incubation only genistein provoked effects on the dynamic phenotype and decreased invasiveness. The effect was more evident in PC-3 cells compared to LNCaP cells. Our results imply that (1) invasive activity is at least partially dependent on membrane fluidity, (2) genistein may exert its antimetastatic effects by changing the mechanical properties of prostate cancer cells and (3) daidzein should be applied at higher concentrations than genistein in order to achieve pharmacological effects.
PB  - Springer, New York
T2  - Journal of Membrane Biology
T1  - Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones
EP  - 314
IS  - 4
SP  - 307
VL  - 246
DO  - 10.1007/s00232-013-9531-1
ER  - 

@article{
author = "Ajdzanović, Vladimir Z and Mojic, Marija and Maksimović-Ivanić, Danijela and Bulatović, Mirna Z and Mijatović, Sanja and Milošević, Verica Lj. and Spasojević, Ivan",
year = "2013",
abstract = "Soy isoflavones represent hopeful unconventional remedies in the therapy of prostate cancer. The aim of our study was to determine the effects of genistein and daidzein on the parameters that reflect metastatic potential, membrane fluidity, invasiveness and dynamic phenotype in Matrigel of LNCaP and PC-3 prostate cancer cells. Cell viability tests, using a wide range of concentrations of soy isoflavones (6-75 mu g/ml for 72 h), were conducted to determine their IC50 concentrations. Electron paramagnetic resonance investigations of prostate cancer cell membrane fluidity were performed at IC50 concentrations of genistein and daidzein (12.5 and 25 mu g/ml, respectively, for 10 min). Genistein provoked significant increases in the membrane order parameter (which is reciprocally proportional to membrane fluidity) of 0.722 +/- A 0.006 (LNCaP), 0.753 +/- A 0.010 (LNCaP + genistein), 0.723 +/- A 0.007 (PC-3) and 0.741 +/- A 0.004 (PC-3 + genistein); however, no such effects were observed for daidzein. While both genistein and daidzein reduced the proliferation of prostate cancer cells at their respective IC50 concentrations, during the 72 h of incubation only genistein provoked effects on the dynamic phenotype and decreased invasiveness. The effect was more evident in PC-3 cells compared to LNCaP cells. Our results imply that (1) invasive activity is at least partially dependent on membrane fluidity, (2) genistein may exert its antimetastatic effects by changing the mechanical properties of prostate cancer cells and (3) daidzein should be applied at higher concentrations than genistein in order to achieve pharmacological effects.",
publisher = "Springer, New York",
journal = "Journal of Membrane Biology",
title = "Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones",
pages = "314-307",
number = "4",
volume = "246",
doi = "10.1007/s00232-013-9531-1"
}

Ajdzanović, V. Z., Mojic, M., Maksimović-Ivanić, D., Bulatović, M. Z., Mijatović, S., Milošević, V. Lj.,& Spasojević, I.. (2013). Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones. in Journal of Membrane Biology
Springer, New York., 246(4), 307-314.
https://doi.org/10.1007/s00232-013-9531-1

Ajdzanović VZ, Mojic M, Maksimović-Ivanić D, Bulatović MZ, Mijatović S, Milošević VL, Spasojević I. Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones. in Journal of Membrane Biology. 2013;246(4):307-314.
doi:10.1007/s00232-013-9531-1 .

Ajdzanović, Vladimir Z, Mojic, Marija, Maksimović-Ivanić, Danijela, Bulatović, Mirna Z, Mijatović, Sanja, Milošević, Verica Lj., Spasojević, Ivan, "Membrane Fluidity, Invasiveness and Dynamic Phenotype of Metastatic Prostate Cancer Cells after Treatment with Soy Isoflavones" in Journal of Membrane Biology, 246, no. 4 (2013):307-314,
https://doi.org/10.1007/s00232-013-9531-1 . .

TY  - JOUR
AU  - Sonmez, Melda
AU  - Ince, Huseyin Yavuz
AU  - Yalcin, Ozlem
AU  - Ajdzanović, Vladimir Z
AU  - Spasojević, Ivan
AU  - Meiselman, Herbert J
AU  - Baskurt, Oguz K
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/726
AB  - The role of membrane fluidity in determining red blood cell (RBC) deformability has been suggested by a number of studies. The present investigation evaluated alterations of RBC membrane fluidity, deformability and stability in the presence of four linear alcohols (methanol, ethanol, propanol and butanol) using ektacytometry and electron paramagnetic resonance (EPR) spectroscopy. All alcohols had a biphasic effect on deformability such that it increased then decreased with increasing concentration; the critical concentration for reversal was an inverse function of molecular size. EPR results showed biphasic changes of near-surface fluidity (i.e., increase then decrease) and a decreased fluidity of the lipid core; rank order of effectiveness was butanol > propanol > ethanol > methanol, with a significant correlation between near-surface fluidity and deformability (r = 0.697; p lt 0.01). The presence of alcohol enhanced the impairment of RBC deformability caused by subjecting cells to 100 Pa shear stress for 300 s, with significant differences from control being observed at higher concentrations of all four alcohols. The level of hemolysis was dependent on molecular size and concentration, whereas echinocytic shape transformation (i.e., biconcave disc to crenated morphology) was observed only for ethanol and propanol. These results are in accordance with available data obtained on model membranes. They document the presence of mechanical links between RBC deformability and near-surface membrane fluidity, chain length-dependence of the ability of alcohols to alter RBC mechanical behavior, and the biphasic response of RBC deformability and near-surface membrane fluidity to increasing alcohol concentrations.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size
IS  - 9
VL  - 8
DO  - 10.1371/journal.pone.0076579
ER  - 

@article{
author = "Sonmez, Melda and Ince, Huseyin Yavuz and Yalcin, Ozlem and Ajdzanović, Vladimir Z and Spasojević, Ivan and Meiselman, Herbert J and Baskurt, Oguz K",
year = "2013",
abstract = "The role of membrane fluidity in determining red blood cell (RBC) deformability has been suggested by a number of studies. The present investigation evaluated alterations of RBC membrane fluidity, deformability and stability in the presence of four linear alcohols (methanol, ethanol, propanol and butanol) using ektacytometry and electron paramagnetic resonance (EPR) spectroscopy. All alcohols had a biphasic effect on deformability such that it increased then decreased with increasing concentration; the critical concentration for reversal was an inverse function of molecular size. EPR results showed biphasic changes of near-surface fluidity (i.e., increase then decrease) and a decreased fluidity of the lipid core; rank order of effectiveness was butanol > propanol > ethanol > methanol, with a significant correlation between near-surface fluidity and deformability (r = 0.697; p lt 0.01). The presence of alcohol enhanced the impairment of RBC deformability caused by subjecting cells to 100 Pa shear stress for 300 s, with significant differences from control being observed at higher concentrations of all four alcohols. The level of hemolysis was dependent on molecular size and concentration, whereas echinocytic shape transformation (i.e., biconcave disc to crenated morphology) was observed only for ethanol and propanol. These results are in accordance with available data obtained on model membranes. They document the presence of mechanical links between RBC deformability and near-surface membrane fluidity, chain length-dependence of the ability of alcohols to alter RBC mechanical behavior, and the biphasic response of RBC deformability and near-surface membrane fluidity to increasing alcohol concentrations.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size",
number = "9",
volume = "8",
doi = "10.1371/journal.pone.0076579"
}

Sonmez, M., Ince, H. Y., Yalcin, O., Ajdzanović, V. Z., Spasojević, I., Meiselman, H. J.,& Baskurt, O. K.. (2013). The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size. in PLoS One
Public Library Science, San Francisco., 8(9).
https://doi.org/10.1371/journal.pone.0076579

Sonmez M, Ince HY, Yalcin O, Ajdzanović VZ, Spasojević I, Meiselman HJ, Baskurt OK. The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size. in PLoS One. 2013;8(9).
doi:10.1371/journal.pone.0076579 .

Sonmez, Melda, Ince, Huseyin Yavuz, Yalcin, Ozlem, Ajdzanović, Vladimir Z, Spasojević, Ivan, Meiselman, Herbert J, Baskurt, Oguz K, "The Effect of Alcohols on Red Blood Cell Mechanical Properties and Membrane Fluidity Depends on Their Molecular Size" in PLoS One, 8, no. 9 (2013),
https://doi.org/10.1371/journal.pone.0076579 . .

TY  - JOUR
AU  - Ajdzanović, Vladimir Z
AU  - Milošević, Verica Lj.
AU  - Spasojević, Ivan
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/581
AB  - Advanced age is often accompanied by glucocorticoid excess which contributes to the pathogenesis of the metabolic syndrome associated with some hemodynamic disorders. Impaired central regulation of stress hormones secretion and increased glucocorticoids/adrenal androgens ratio trigger hyperglycemia, elevated blood lipids and visceral fat accumulation, associated with hypertension and increased blood viscosity, all of which represent cardiovascular morbidity factors in this age. Finding the adequate therapeutic solutions is set as an imperative in the treatment of listed symptoms. Biologically active soy isoflavones, exhibiting estrogen- and membrane-receptor agonistic/antagonistic activity, and antioxidative and tyrosine kinase/steroidogenic enzyme inhibiting effects, appear as alternative therapeutics for various ageing-related diseases. It has been shown that soy isoflavones reduce some of the listed risk factors, while affecting the hemodynamic group of cardiovascular parameters directly, as well as indirectly via endocrine perturbations. Soy isoflavones may reverse the glucocorticoids/adrenal androgens ratio, lower serum cholesterol, slow the development of atherosclerotic plaque formation, inhibit platelet aggregation, increase cardiac contractility, but they may have diverse effects on blood viscosity and may increase triglyceride levels. Herein, we present the projection of soy isoflavones-based therapy of glucocorticoid excess and disturbed hemodynamics in advanced age, concluding that although promising, it requires the impartial approach and certain precautions.
PB  - General Physiol And Biophysics, Bratislava
T2  - General Physiology and Biophysics
T1  - Glucocorticoid excess and disturbed hemodynamics in advanced age: the extent to which soy isoflavones may be beneficial
EP  - 374
IS  - 4
SP  - 367
VL  - 31
DO  - 10.4149/gpb_2012_041
ER  - 

@article{
author = "Ajdzanović, Vladimir Z and Milošević, Verica Lj. and Spasojević, Ivan",
year = "2012",
abstract = "Advanced age is often accompanied by glucocorticoid excess which contributes to the pathogenesis of the metabolic syndrome associated with some hemodynamic disorders. Impaired central regulation of stress hormones secretion and increased glucocorticoids/adrenal androgens ratio trigger hyperglycemia, elevated blood lipids and visceral fat accumulation, associated with hypertension and increased blood viscosity, all of which represent cardiovascular morbidity factors in this age. Finding the adequate therapeutic solutions is set as an imperative in the treatment of listed symptoms. Biologically active soy isoflavones, exhibiting estrogen- and membrane-receptor agonistic/antagonistic activity, and antioxidative and tyrosine kinase/steroidogenic enzyme inhibiting effects, appear as alternative therapeutics for various ageing-related diseases. It has been shown that soy isoflavones reduce some of the listed risk factors, while affecting the hemodynamic group of cardiovascular parameters directly, as well as indirectly via endocrine perturbations. Soy isoflavones may reverse the glucocorticoids/adrenal androgens ratio, lower serum cholesterol, slow the development of atherosclerotic plaque formation, inhibit platelet aggregation, increase cardiac contractility, but they may have diverse effects on blood viscosity and may increase triglyceride levels. Herein, we present the projection of soy isoflavones-based therapy of glucocorticoid excess and disturbed hemodynamics in advanced age, concluding that although promising, it requires the impartial approach and certain precautions.",
publisher = "General Physiol And Biophysics, Bratislava",
journal = "General Physiology and Biophysics",
title = "Glucocorticoid excess and disturbed hemodynamics in advanced age: the extent to which soy isoflavones may be beneficial",
pages = "374-367",
number = "4",
volume = "31",
doi = "10.4149/gpb_2012_041"
}

Ajdzanović, V. Z., Milošević, V. Lj.,& Spasojević, I.. (2012). Glucocorticoid excess and disturbed hemodynamics in advanced age: the extent to which soy isoflavones may be beneficial. in General Physiology and Biophysics
General Physiol And Biophysics, Bratislava., 31(4), 367-374.
https://doi.org/10.4149/gpb_2012_041

Ajdzanović VZ, Milošević VL, Spasojević I. Glucocorticoid excess and disturbed hemodynamics in advanced age: the extent to which soy isoflavones may be beneficial. in General Physiology and Biophysics. 2012;31(4):367-374.
doi:10.4149/gpb_2012_041 .

Ajdzanović, Vladimir Z, Milošević, Verica Lj., Spasojević, Ivan, "Glucocorticoid excess and disturbed hemodynamics in advanced age: the extent to which soy isoflavones may be beneficial" in General Physiology and Biophysics, 31, no. 4 (2012):367-374,
https://doi.org/10.4149/gpb_2012_041 . .

TY  - JOUR
AU  - Ajdzanović, Vladimir Z
AU  - Spasojević, Ivan
AU  - Pantelic, Jasmina B
AU  - Sosic-Jurjević, Branka T
AU  - Filipović, Branko R
AU  - Milošević, Verica Lj.
AU  - Severs, Walter
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/547
AB  - Fluidnost membrane eritrocita uslovljava njihove reološke karakteristike, svojevrsne dinamičke kvalitete, izmenjene u hipertenziji i aterosklerozi. Rizik od navedenih i drugih kardiovaskularnih oboljenja se kod žena povećava sa starenjem. Tegobe koje prate menopauzu se obično ublažavaju supstitucionom hormonskom terapijom, koja može povećati rizik od pojave malignih oboljenja. Esencijalno ulje biljke Vitex agnus-castus L. sadrži različita organska jedinjenja (monoterpene, seskviterpene i terpenoide) i sve češće se koristi kao alternativno terapijsko sredstvo za simptome menopauze. Navedene komponente esencijalnog ulja mogu se ugraditi u ćelijske membrane i menjati njihovu fluidnost. Cilj studije bio je da se utvrde efekti esencijalnog ulja biljke Vitex agnus-castus na fluidnost membrane eritrocita po njenoj du bini. Korišćena je spektroskopija elektronske paramagnetne rezonance i masnokiselinske spinske probe (5-doksil stearinska i 12-doksil stearinska kiselina), čiji spektri za vise od fluidnosti membrane. Nakon tretmana eritrocita esencijalnim uljem biljke Vitex agnus-castus utvrđeno je značajno (p=0,029) i reverzibilno povećanje fluidnosti njihove membrane u dubljim, hidrofobnim regionima, dok u površinskim, hidrofilnim regionima eritrocitne membrane nisu utvrđene značajne promene (p>0,05). Ovi rezultati ukazuju na povećanje fluidnosti eritrocitne membrane nakon tretmana esencijalnim uljem biljke Vitex agnus-castus, što može biti koristan efekat pri tretmanu pacijentkinja sa hipertenzijom i drugim kardiovaskularnim oboljenjima u menopauzi.
AB  - Erythrocyte membrane fluidity is related to their rheologic behavior, the dynamic quality of erythrocytes, which is tempted in hypertension and atherosclerosis. An increased risk of these and other cardiovascular diseases occurs in ageing women. Menopause-related conditions are often treated with hormone replacement therapy that may increase the risk of malignancies. Vitex agnus-castus L. essential oil contains various organic compounds (monoterpenes, sesquiterpenes and terpenoids), and is increasingly used as an alternative therapy for menopausal symptoms. These components of the oil may be incorporated into cell membranes, thereby changing the membrane fluidity. The aim of this study was to determine the effects of Vitex agnuscastus essential oil on human erythrocyte membrane fluidity at graded depths. We used Electron Paramagnetic Resonance spectroscopy and fatty acid spin probes (5-doxyl stearic acid and 12-doxyl stearic acid), whose spectra depend on membrane fluidity. After treatment with Vitex agnus-castus essential oil the erythrocytes had a significant (p=0.029) and reversible increase in membrane fluidity in the deeper hydrophobic membrane regions, with no change (p>0.05) in fluidity near the membrane's hydrophilic surface. These results document increased fluidity of the human erythrocyte membrane by Vitex agnus-castus essential oil, and this action may be useful in patients with menopause-related hypertension and other cardiovascular conditions.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane
T1  - Vitex agnus-castus L.: Essential oil increases human erythrocyte membrane fluidity
EP  - 227
IS  - 3
SP  - 222
VL  - 31
DO  - 10.2478/v10011-011-0055-x
ER  - 

@article{
author = "Ajdzanović, Vladimir Z and Spasojević, Ivan and Pantelic, Jasmina B and Sosic-Jurjević, Branka T and Filipović, Branko R and Milošević, Verica Lj. and Severs, Walter",
year = "2012",
abstract = "Fluidnost membrane eritrocita uslovljava njihove reološke karakteristike, svojevrsne dinamičke kvalitete, izmenjene u hipertenziji i aterosklerozi. Rizik od navedenih i drugih kardiovaskularnih oboljenja se kod žena povećava sa starenjem. Tegobe koje prate menopauzu se obično ublažavaju supstitucionom hormonskom terapijom, koja može povećati rizik od pojave malignih oboljenja. Esencijalno ulje biljke Vitex agnus-castus L. sadrži različita organska jedinjenja (monoterpene, seskviterpene i terpenoide) i sve češće se koristi kao alternativno terapijsko sredstvo za simptome menopauze. Navedene komponente esencijalnog ulja mogu se ugraditi u ćelijske membrane i menjati njihovu fluidnost. Cilj studije bio je da se utvrde efekti esencijalnog ulja biljke Vitex agnus-castus na fluidnost membrane eritrocita po njenoj du bini. Korišćena je spektroskopija elektronske paramagnetne rezonance i masnokiselinske spinske probe (5-doksil stearinska i 12-doksil stearinska kiselina), čiji spektri za vise od fluidnosti membrane. Nakon tretmana eritrocita esencijalnim uljem biljke Vitex agnus-castus utvrđeno je značajno (p=0,029) i reverzibilno povećanje fluidnosti njihove membrane u dubljim, hidrofobnim regionima, dok u površinskim, hidrofilnim regionima eritrocitne membrane nisu utvrđene značajne promene (p>0,05). Ovi rezultati ukazuju na povećanje fluidnosti eritrocitne membrane nakon tretmana esencijalnim uljem biljke Vitex agnus-castus, što može biti koristan efekat pri tretmanu pacijentkinja sa hipertenzijom i drugim kardiovaskularnim oboljenjima u menopauzi., Erythrocyte membrane fluidity is related to their rheologic behavior, the dynamic quality of erythrocytes, which is tempted in hypertension and atherosclerosis. An increased risk of these and other cardiovascular diseases occurs in ageing women. Menopause-related conditions are often treated with hormone replacement therapy that may increase the risk of malignancies. Vitex agnus-castus L. essential oil contains various organic compounds (monoterpenes, sesquiterpenes and terpenoids), and is increasingly used as an alternative therapy for menopausal symptoms. These components of the oil may be incorporated into cell membranes, thereby changing the membrane fluidity. The aim of this study was to determine the effects of Vitex agnuscastus essential oil on human erythrocyte membrane fluidity at graded depths. We used Electron Paramagnetic Resonance spectroscopy and fatty acid spin probes (5-doxyl stearic acid and 12-doxyl stearic acid), whose spectra depend on membrane fluidity. After treatment with Vitex agnus-castus essential oil the erythrocytes had a significant (p=0.029) and reversible increase in membrane fluidity in the deeper hydrophobic membrane regions, with no change (p>0.05) in fluidity near the membrane's hydrophilic surface. These results document increased fluidity of the human erythrocyte membrane by Vitex agnus-castus essential oil, and this action may be useful in patients with menopause-related hypertension and other cardiovascular conditions.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane, Vitex agnus-castus L.: Essential oil increases human erythrocyte membrane fluidity",
pages = "227-222",
number = "3",
volume = "31",
doi = "10.2478/v10011-011-0055-x"
}

Ajdzanović, V. Z., Spasojević, I., Pantelic, J. B., Sosic-Jurjević, B. T., Filipović, B. R., Milošević, V. Lj.,& Severs, W.. (2012). Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 31(3), 222-227.
https://doi.org/10.2478/v10011-011-0055-x

Ajdzanović VZ, Spasojević I, Pantelic JB, Sosic-Jurjević BT, Filipović BR, Milošević VL, Severs W. Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane. in Journal of Medical Biochemistry. 2012;31(3):222-227.
doi:10.2478/v10011-011-0055-x .

Ajdzanović, Vladimir Z, Spasojević, Ivan, Pantelic, Jasmina B, Sosic-Jurjević, Branka T, Filipović, Branko R, Milošević, Verica Lj., Severs, Walter, "Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane" in Journal of Medical Biochemistry, 31, no. 3 (2012):222-227,
https://doi.org/10.2478/v10011-011-0055-x . .