TY  - JOUR
AU  - Venturelli, Leonardo
AU  - Kohler, Anne-Celine
AU  - Stupar, Petar
AU  - Villalba, Maria, I
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
AU  - Bertacchi, Massimiliano
AU  - Dinarelli, Simone
AU  - Girasole, Marco
AU  - Pesic, Milica
AU  - Banković, Jasna
AU  - Vela, Maria E.
AU  - Yantorno, Osvaldo
AU  - Willaert, Ronnie
AU  - Dietler, Giovanni
AU  - Longo, Giovanni
AU  - Kasas, Sandor
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1338
AB  - The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.
PB  - Wiley, Hoboken
T2  - Journal of Molecular Recognition
T1  - A perspective view on the nanomotion detection of living organisms and its features
IS  - 12
VL  - 33
DO  - 10.1002/jmr.2849
ER  - 

@article{
author = "Venturelli, Leonardo and Kohler, Anne-Celine and Stupar, Petar and Villalba, Maria, I and Kalauzi, Aleksandar and Radotić, Ksenija and Bertacchi, Massimiliano and Dinarelli, Simone and Girasole, Marco and Pesic, Milica and Banković, Jasna and Vela, Maria E. and Yantorno, Osvaldo and Willaert, Ronnie and Dietler, Giovanni and Longo, Giovanni and Kasas, Sandor",
year = "2020",
abstract = "The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro-fabricated cantilever and by monitoring its displacements with an atomic force microscope-based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life-threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.",
publisher = "Wiley, Hoboken",
journal = "Journal of Molecular Recognition",
title = "A perspective view on the nanomotion detection of living organisms and its features",
number = "12",
volume = "33",
doi = "10.1002/jmr.2849"
}

Venturelli, L., Kohler, A., Stupar, P., Villalba, M. I., Kalauzi, A., Radotić, K., Bertacchi, M., Dinarelli, S., Girasole, M., Pesic, M., Banković, J., Vela, M. E., Yantorno, O., Willaert, R., Dietler, G., Longo, G.,& Kasas, S.. (2020). A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition
Wiley, Hoboken., 33(12).
https://doi.org/10.1002/jmr.2849

Venturelli L, Kohler A, Stupar P, Villalba MI, Kalauzi A, Radotić K, Bertacchi M, Dinarelli S, Girasole M, Pesic M, Banković J, Vela ME, Yantorno O, Willaert R, Dietler G, Longo G, Kasas S. A perspective view on the nanomotion detection of living organisms and its features. in Journal of Molecular Recognition. 2020;33(12).
doi:10.1002/jmr.2849 .

Venturelli, Leonardo, Kohler, Anne-Celine, Stupar, Petar, Villalba, Maria, I, Kalauzi, Aleksandar, Radotić, Ksenija, Bertacchi, Massimiliano, Dinarelli, Simone, Girasole, Marco, Pesic, Milica, Banković, Jasna, Vela, Maria E., Yantorno, Osvaldo, Willaert, Ronnie, Dietler, Giovanni, Longo, Giovanni, Kasas, Sandor, "A perspective view on the nanomotion detection of living organisms and its features" in Journal of Molecular Recognition, 33, no. 12 (2020),
https://doi.org/10.1002/jmr.2849 . .

TY  - JOUR
AU  - Pesic, Milica
AU  - Podolski-Renic, Ana
AU  - Stojković, Sonja
AU  - Matović, Branko
AU  - Zmejkoski, Danica
AU  - Kojić, Vesna
AU  - Bogdanović, Gordana
AU  - Pavicević, Aleksandra
AU  - Mojović, Miloš
AU  - Savić, Aleksandar G
AU  - Milenković, Ivana
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/931
AB  - Data on medical applications of cerium oxide nanoparticles CeO2 (CONP) are promising, yet information regarding their action in cells is incomplete and there are conflicting reports about in vitro toxicity. Herein, we have studied cytotoxic effect of CONP in several cancer and normal cell lines and their potential to change intracellular redox status. The IC50 was achieved only in two of eight tested cell lines, melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating room temperature method was applied to produce CONP with an average crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2- vacancies. The induction of cell death by CONP and the production of reactive oxygen species (ROS) were analyzed by flow-cytometry. Free radicals related antioxidant capacity of the cells was studied by the reduction of stable free radical TEMPONE using electron spin resonance spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5) were insensitive. The most sensitive were 518A2 melanoma and HT-29 colorectal adenocarcinoma cell lines, with the IC50 values being between 100 and 200 mu M. Decreased rate of TEMPONE reduction and increased production of certain ROS species (peroxynitrite and hydrogen peroxide anion) indicates that free radical metabolism, thus redox status was changed, and antioxidant capacity damaged in the CONP treated 518A2 and HT-29 cells. In conclusion, changes in intracellular redox status induced by CONP are partly attributed to the prooxidant activity of the nanoparticles. Further, ROS induced cell damages might eventually lead to the cell death. However, low inhibitory potential of CONP in the other human cell lines tested indicates that CONP may be safe for human usage in industry and medicine.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity
EP  - 93
SP  - 85
VL  - 232
DO  - 10.1016/j.cbi.2015.03.013
ER  - 

@article{
author = "Pesic, Milica and Podolski-Renic, Ana and Stojković, Sonja and Matović, Branko and Zmejkoski, Danica and Kojić, Vesna and Bogdanović, Gordana and Pavicević, Aleksandra and Mojović, Miloš and Savić, Aleksandar G and Milenković, Ivana and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2015",
abstract = "Data on medical applications of cerium oxide nanoparticles CeO2 (CONP) are promising, yet information regarding their action in cells is incomplete and there are conflicting reports about in vitro toxicity. Herein, we have studied cytotoxic effect of CONP in several cancer and normal cell lines and their potential to change intracellular redox status. The IC50 was achieved only in two of eight tested cell lines, melanoma 518A2 and colorectal adenocarcinoma HT-29. Self-propagating room temperature method was applied to produce CONP with an average crystalline size of 4 nm. The results confirmed presence of Ce3+ and O2- vacancies. The induction of cell death by CONP and the production of reactive oxygen species (ROS) were analyzed by flow-cytometry. Free radicals related antioxidant capacity of the cells was studied by the reduction of stable free radical TEMPONE using electron spin resonance spectroscopy. CONP showed low or moderate cytotoxicity in cancer cell lines: adenocarcinoma DLD1 and multi-drug resistant DLD1-TxR, non-small cell lung carcinoma NCI-H460 and multi-drug resistant NCI-H460/R, while normal cell lines (keratinocytes HaCaT, lung fetal fibroblasts MRC-5) were insensitive. The most sensitive were 518A2 melanoma and HT-29 colorectal adenocarcinoma cell lines, with the IC50 values being between 100 and 200 mu M. Decreased rate of TEMPONE reduction and increased production of certain ROS species (peroxynitrite and hydrogen peroxide anion) indicates that free radical metabolism, thus redox status was changed, and antioxidant capacity damaged in the CONP treated 518A2 and HT-29 cells. In conclusion, changes in intracellular redox status induced by CONP are partly attributed to the prooxidant activity of the nanoparticles. Further, ROS induced cell damages might eventually lead to the cell death. However, low inhibitory potential of CONP in the other human cell lines tested indicates that CONP may be safe for human usage in industry and medicine.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity",
pages = "93-85",
volume = "232",
doi = "10.1016/j.cbi.2015.03.013"
}

Pesic, M., Podolski-Renic, A., Stojković, S., Matović, B., Zmejkoski, D., Kojić, V., Bogdanović, G., Pavicević, A., Mojović, M., Savić, A. G., Milenković, I., Kalauzi, A.,& Radotić, K.. (2015). Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 232, 85-93.
https://doi.org/10.1016/j.cbi.2015.03.013

Pesic M, Podolski-Renic A, Stojković S, Matović B, Zmejkoski D, Kojić V, Bogdanović G, Pavicević A, Mojović M, Savić AG, Milenković I, Kalauzi A, Radotić K. Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity. in Chemico-Biological Interactions. 2015;232:85-93.
doi:10.1016/j.cbi.2015.03.013 .

Pesic, Milica, Podolski-Renic, Ana, Stojković, Sonja, Matović, Branko, Zmejkoski, Danica, Kojić, Vesna, Bogdanović, Gordana, Pavicević, Aleksandra, Mojović, Miloš, Savić, Aleksandar G, Milenković, Ivana, Kalauzi, Aleksandar, Radotić, Ksenija, "Anti-cancer effects of cerium oxide nanoparticles and its intracellular redox activity" in Chemico-Biological Interactions, 232 (2015):85-93,
https://doi.org/10.1016/j.cbi.2015.03.013 . .