TY  - JOUR
AU  - Sunjog, Karolina
AU  - Cirković, Sanja
AU  - Vukovic-Gacic, Branka
AU  - Guc-Scekic, Marija
AU  - Misković, Marijana
AU  - Vujic, Dragana
AU  - Skoric, Dejan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1217
AB  - Fanconi anemia (FA) is a complex genetic disease with a variety of congenital and hematological symptoms, including the predisposition for cancer development. The main hallmark of FA cells, an increased chromosomal fragility, in the presence of the DNA-interstrand cross-linking chemicals, mitomycin C or diepoxybutane (DEB), makes the diagnosis of FA much easier. Cytogenetic method can detect the FA patients with highly elevated chromosomal breakage, but also some of the patients with borderline sensitivity to DEB no matter if they have FA or not. These particular circumstances lead us to introduce comet assay along with cytogenetic analysis, in order to determine DNA lesions and chromosomal fragility in untreated and DEB-treated lymphocytes of full blood from seven patients with clinical features of FA. Highly elevated DEB induced chromosomal sensitivity confirmed the diagnosis in five patients (FA group: 0.48-4.47 breaks/cell vs control group: 0.00-0.08 breaks/cell). Borderline DEB sensitivity (FA* group: 0.15-0.44 breaks/cell) was found in the remaining two patients. Results of the comet assay showed higher baseline and DEB-induced DNA damage values (Olive tail moment and tail intensity) in all five FA and one FA* patient, when compared to the control group. These findings could provide a new model of FA screening test algorithm, including comet assay as additional and very useful accurate tool, beside the DEB test, in differential diagnosis of FA.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Comet assay and cytogenetic findings in differential diagnosis of fanconi anemia
EP  - 1126
IS  - 3
SP  - 1113
VL  - 51
DO  - 10.2298/GENSR1903113S
ER  - 

@article{
author = "Sunjog, Karolina and Cirković, Sanja and Vukovic-Gacic, Branka and Guc-Scekic, Marija and Misković, Marijana and Vujic, Dragana and Skoric, Dejan",
year = "2019",
abstract = "Fanconi anemia (FA) is a complex genetic disease with a variety of congenital and hematological symptoms, including the predisposition for cancer development. The main hallmark of FA cells, an increased chromosomal fragility, in the presence of the DNA-interstrand cross-linking chemicals, mitomycin C or diepoxybutane (DEB), makes the diagnosis of FA much easier. Cytogenetic method can detect the FA patients with highly elevated chromosomal breakage, but also some of the patients with borderline sensitivity to DEB no matter if they have FA or not. These particular circumstances lead us to introduce comet assay along with cytogenetic analysis, in order to determine DNA lesions and chromosomal fragility in untreated and DEB-treated lymphocytes of full blood from seven patients with clinical features of FA. Highly elevated DEB induced chromosomal sensitivity confirmed the diagnosis in five patients (FA group: 0.48-4.47 breaks/cell vs control group: 0.00-0.08 breaks/cell). Borderline DEB sensitivity (FA* group: 0.15-0.44 breaks/cell) was found in the remaining two patients. Results of the comet assay showed higher baseline and DEB-induced DNA damage values (Olive tail moment and tail intensity) in all five FA and one FA* patient, when compared to the control group. These findings could provide a new model of FA screening test algorithm, including comet assay as additional and very useful accurate tool, beside the DEB test, in differential diagnosis of FA.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Comet assay and cytogenetic findings in differential diagnosis of fanconi anemia",
pages = "1126-1113",
number = "3",
volume = "51",
doi = "10.2298/GENSR1903113S"
}

Sunjog, K., Cirković, S., Vukovic-Gacic, B., Guc-Scekic, M., Misković, M., Vujic, D.,& Skoric, D.. (2019). Comet assay and cytogenetic findings in differential diagnosis of fanconi anemia. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 51(3), 1113-1126.
https://doi.org/10.2298/GENSR1903113S

Sunjog K, Cirković S, Vukovic-Gacic B, Guc-Scekic M, Misković M, Vujic D, Skoric D. Comet assay and cytogenetic findings in differential diagnosis of fanconi anemia. in Genetika-Belgrade. 2019;51(3):1113-1126.
doi:10.2298/GENSR1903113S .

Sunjog, Karolina, Cirković, Sanja, Vukovic-Gacic, Branka, Guc-Scekic, Marija, Misković, Marijana, Vujic, Dragana, Skoric, Dejan, "Comet assay and cytogenetic findings in differential diagnosis of fanconi anemia" in Genetika-Belgrade, 51, no. 3 (2019):1113-1126,
https://doi.org/10.2298/GENSR1903113S . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Stanimirović, Bojana
AU  - Đorđević, Neda O
AU  - Hegediš, Aleksandar
AU  - Karaman, Ivo M
AU  - Horvatović, Mladen
AU  - Radotić, Ksenija
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/651
AB  - The objective of the present study was in vitro evaluation of radioprotective activity of the freshwater bryozoan Hyalinella punctata on cultured human peripheral blood lymphocytes after irradiation with 2 gy of Co-60 gamma-rays. Since its water extract at concentration 0.001 mg/mL reduced the incidence of radiation-induced micronuclei for almost 30 %, it could be considered as a promising source of new natural products with the aforementined activity. Both the content of sulphur (1.17%) determined by gravimetric method and infrared absorption frequences (76 % similarity with those of bacitracin) of the investigated extract indicate the presence of organic sulphur compound(s) such as cyclic peptides and polypeptides which might be responsible for the observed radioprotection
PB  - Asian Journal Of Chemistry, Sahibabad
T2  - Asian Journal of Chemistry
T1  - In vitro Radioprotective Activity of the Bryozoan Hyalinella punctata
EP  - 4714
IS  - 8
SP  - 4713
VL  - 25
DO  - 10.14233/ajchem.2013.14917
ER  - 

@article{
author = "Pejin, Boris and Stanimirović, Bojana and Đorđević, Neda O and Hegediš, Aleksandar and Karaman, Ivo M and Horvatović, Mladen and Radotić, Ksenija",
year = "2013",
abstract = "The objective of the present study was in vitro evaluation of radioprotective activity of the freshwater bryozoan Hyalinella punctata on cultured human peripheral blood lymphocytes after irradiation with 2 gy of Co-60 gamma-rays. Since its water extract at concentration 0.001 mg/mL reduced the incidence of radiation-induced micronuclei for almost 30 %, it could be considered as a promising source of new natural products with the aforementined activity. Both the content of sulphur (1.17%) determined by gravimetric method and infrared absorption frequences (76 % similarity with those of bacitracin) of the investigated extract indicate the presence of organic sulphur compound(s) such as cyclic peptides and polypeptides which might be responsible for the observed radioprotection",
publisher = "Asian Journal Of Chemistry, Sahibabad",
journal = "Asian Journal of Chemistry",
title = "In vitro Radioprotective Activity of the Bryozoan Hyalinella punctata",
pages = "4714-4713",
number = "8",
volume = "25",
doi = "10.14233/ajchem.2013.14917"
}

Pejin, B., Stanimirović, B., Đorđević, N. O., Hegediš, A., Karaman, I. M., Horvatović, M.,& Radotić, K.. (2013). In vitro Radioprotective Activity of the Bryozoan Hyalinella punctata. in Asian Journal of Chemistry
Asian Journal Of Chemistry, Sahibabad., 25(8), 4713-4714.
https://doi.org/10.14233/ajchem.2013.14917

Pejin B, Stanimirović B, Đorđević NO, Hegediš A, Karaman IM, Horvatović M, Radotić K. In vitro Radioprotective Activity of the Bryozoan Hyalinella punctata. in Asian Journal of Chemistry. 2013;25(8):4713-4714.
doi:10.14233/ajchem.2013.14917 .

Pejin, Boris, Stanimirović, Bojana, Đorđević, Neda O, Hegediš, Aleksandar, Karaman, Ivo M, Horvatović, Mladen, Radotić, Ksenija, "In vitro Radioprotective Activity of the Bryozoan Hyalinella punctata" in Asian Journal of Chemistry, 25, no. 8 (2013):4713-4714,
https://doi.org/10.14233/ajchem.2013.14917 . .