TY  - JOUR
AU  - Harhaji-Trajković, Ljubica M.
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Stojanović, Ivana D.
AU  - Momcilović, Miljana
AU  - Tufegdžić, Srđan J.
AU  - Maksimović, Vuk
AU  - Marjanović, Žaklina
AU  - Stosic-Grujicic, Stanislava
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/347
AB  - Anticancer activities of various extracts of the medicinal mushroom, Ganoderma lucidum, have been widely demonstrated and are mainly associated with the presence of different bioactive polysaccharides and triterpenoids. We have evaluated and compared in vitro and in vivo the antitumor effects of two preparations from Ganoderma lucidum: a methanol extract containing total terpenoids (GLme) and a purified methanol extract containing mainly acidic terpenoids (GLpme). Both extracts inhibited tumor growth of B16 mouse melanoma cells inoculated subcutaneously into syngeneic C57BL/6 mice and reduced viability of B16 cells in vitro, whereby GLme exhibited stronger effect. Furthermore, anticancer activity of GLme was demonstrated for the first time against two other rodent tumor cell lines, L929-mouse fibrosarcoma and C6-rat astrocytoma. The mechanism of antitumor activity of GLme comprised inhibition of cell proliferation and induction of caspase-dependent apoptotic cell death mediated by upregulated p53 and inhibited Bcl-2 expression. Moreover, the antitumor effect of the GLme was associated with intensified production of reactive oxygen species, whereas their neutralization by the antioxidant, N-acetyl cysteine, resulted in partial recovery of cell viability. Thus, our results suggest that GLme might be a good candidate for treatment of diverse forms of cancers.
PB  - Routledge Journals, Taylor & Francis Ltd, Abingdon
T2  - Nutrition and Cancer-An International Journal
T1  - Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo
EP  - 707
IS  - 5
SP  - 696
VL  - 61
DO  - 10.1080/01635580902898743
ER  - 

@article{
author = "Harhaji-Trajković, Ljubica M. and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, Ivana D. and Momcilović, Miljana and Tufegdžić, Srđan J. and Maksimović, Vuk and Marjanović, Žaklina and Stosic-Grujicic, Stanislava",
year = "2009",
abstract = "Anticancer activities of various extracts of the medicinal mushroom, Ganoderma lucidum, have been widely demonstrated and are mainly associated with the presence of different bioactive polysaccharides and triterpenoids. We have evaluated and compared in vitro and in vivo the antitumor effects of two preparations from Ganoderma lucidum: a methanol extract containing total terpenoids (GLme) and a purified methanol extract containing mainly acidic terpenoids (GLpme). Both extracts inhibited tumor growth of B16 mouse melanoma cells inoculated subcutaneously into syngeneic C57BL/6 mice and reduced viability of B16 cells in vitro, whereby GLme exhibited stronger effect. Furthermore, anticancer activity of GLme was demonstrated for the first time against two other rodent tumor cell lines, L929-mouse fibrosarcoma and C6-rat astrocytoma. The mechanism of antitumor activity of GLme comprised inhibition of cell proliferation and induction of caspase-dependent apoptotic cell death mediated by upregulated p53 and inhibited Bcl-2 expression. Moreover, the antitumor effect of the GLme was associated with intensified production of reactive oxygen species, whereas their neutralization by the antioxidant, N-acetyl cysteine, resulted in partial recovery of cell viability. Thus, our results suggest that GLme might be a good candidate for treatment of diverse forms of cancers.",
publisher = "Routledge Journals, Taylor & Francis Ltd, Abingdon",
journal = "Nutrition and Cancer-An International Journal",
title = "Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo",
pages = "707-696",
number = "5",
volume = "61",
doi = "10.1080/01635580902898743"
}

Harhaji-Trajković, L. M., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I. D., Momcilović, M., Tufegdžić, S. J., Maksimović, V., Marjanović, Ž.,& Stosic-Grujicic, S.. (2009). Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo. in Nutrition and Cancer-An International Journal
Routledge Journals, Taylor & Francis Ltd, Abingdon., 61(5), 696-707.
https://doi.org/10.1080/01635580902898743

Harhaji-Trajković LM, Mijatović S, Maksimović-Ivanić D, Stojanović ID, Momcilović M, Tufegdžić SJ, Maksimović V, Marjanović Ž, Stosic-Grujicic S. Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo. in Nutrition and Cancer-An International Journal. 2009;61(5):696-707.
doi:10.1080/01635580902898743 .

Harhaji-Trajković, Ljubica M., Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, Ivana D., Momcilović, Miljana, Tufegdžić, Srđan J., Maksimović, Vuk, Marjanović, Žaklina, Stosic-Grujicic, Stanislava, "Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo" in Nutrition and Cancer-An International Journal, 61, no. 5 (2009):696-707,
https://doi.org/10.1080/01635580902898743 . .

TY  - JOUR
AU  - Harhaji, Lj.
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Stojanović, I.
AU  - Momcilović, Miljana
AU  - Maksimović, Vuk
AU  - Tufegdžić, Srđan J.
AU  - Marjanović, Žaklina
AU  - Mostarica-Stojković, M.
AU  - Vučinić, Željko
AU  - Stosic-Grujicic, Stanislava
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/262
AB  - Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study
EP  - 1833
IS  - 5
SP  - 1825
VL  - 46
DO  - 10.1016/j.fct.2008.01.027
ER  - 

@article{
author = "Harhaji, Lj. and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, I. and Momcilović, Miljana and Maksimović, Vuk and Tufegdžić, Srđan J. and Marjanović, Žaklina and Mostarica-Stojković, M. and Vučinić, Željko and Stosic-Grujicic, Stanislava",
year = "2008",
abstract = "Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study",
pages = "1833-1825",
number = "5",
volume = "46",
doi = "10.1016/j.fct.2008.01.027"
}

Harhaji, Lj., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I., Momcilović, M., Maksimović, V., Tufegdžić, S. J., Marjanović, Ž., Mostarica-Stojković, M., Vučinić, Ž.,& Stosic-Grujicic, S.. (2008). Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 46(5), 1825-1833.
https://doi.org/10.1016/j.fct.2008.01.027

Harhaji L, Mijatović S, Maksimović-Ivanić D, Stojanović I, Momcilović M, Maksimović V, Tufegdžić SJ, Marjanović Ž, Mostarica-Stojković M, Vučinić Ž, Stosic-Grujicic S. Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology. 2008;46(5):1825-1833.
doi:10.1016/j.fct.2008.01.027 .

Harhaji, Lj., Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, I., Momcilović, Miljana, Maksimović, Vuk, Tufegdžić, Srđan J., Marjanović, Žaklina, Mostarica-Stojković, M., Vučinić, Željko, Stosic-Grujicic, Stanislava, "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study" in Food and Chemical Toxicology, 46, no. 5 (2008):1825-1833,
https://doi.org/10.1016/j.fct.2008.01.027 . .