TY  - JOUR
AU  - Ajdzanović, Vladimir Z
AU  - Spasojević, Ivan
AU  - Pantelic, Jasmina B
AU  - Sosic-Jurjević, Branka T
AU  - Filipović, Branko R
AU  - Milošević, Verica Lj.
AU  - Severs, Walter
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/547
AB  - Fluidnost membrane eritrocita uslovljava njihove reološke karakteristike, svojevrsne dinamičke kvalitete, izmenjene u hipertenziji i aterosklerozi. Rizik od navedenih i drugih kardiovaskularnih oboljenja se kod žena povećava sa starenjem. Tegobe koje prate menopauzu se obično ublažavaju supstitucionom hormonskom terapijom, koja može povećati rizik od pojave malignih oboljenja. Esencijalno ulje biljke Vitex agnus-castus L. sadrži različita organska jedinjenja (monoterpene, seskviterpene i terpenoide) i sve češće se koristi kao alternativno terapijsko sredstvo za simptome menopauze. Navedene komponente esencijalnog ulja mogu se ugraditi u ćelijske membrane i menjati njihovu fluidnost. Cilj studije bio je da se utvrde efekti esencijalnog ulja biljke Vitex agnus-castus na fluidnost membrane eritrocita po njenoj du bini. Korišćena je spektroskopija elektronske paramagnetne rezonance i masnokiselinske spinske probe (5-doksil stearinska i 12-doksil stearinska kiselina), čiji spektri za vise od fluidnosti membrane. Nakon tretmana eritrocita esencijalnim uljem biljke Vitex agnus-castus utvrđeno je značajno (p=0,029) i reverzibilno povećanje fluidnosti njihove membrane u dubljim, hidrofobnim regionima, dok u površinskim, hidrofilnim regionima eritrocitne membrane nisu utvrđene značajne promene (p>0,05). Ovi rezultati ukazuju na povećanje fluidnosti eritrocitne membrane nakon tretmana esencijalnim uljem biljke Vitex agnus-castus, što može biti koristan efekat pri tretmanu pacijentkinja sa hipertenzijom i drugim kardiovaskularnim oboljenjima u menopauzi.
AB  - Erythrocyte membrane fluidity is related to their rheologic behavior, the dynamic quality of erythrocytes, which is tempted in hypertension and atherosclerosis. An increased risk of these and other cardiovascular diseases occurs in ageing women. Menopause-related conditions are often treated with hormone replacement therapy that may increase the risk of malignancies. Vitex agnus-castus L. essential oil contains various organic compounds (monoterpenes, sesquiterpenes and terpenoids), and is increasingly used as an alternative therapy for menopausal symptoms. These components of the oil may be incorporated into cell membranes, thereby changing the membrane fluidity. The aim of this study was to determine the effects of Vitex agnuscastus essential oil on human erythrocyte membrane fluidity at graded depths. We used Electron Paramagnetic Resonance spectroscopy and fatty acid spin probes (5-doxyl stearic acid and 12-doxyl stearic acid), whose spectra depend on membrane fluidity. After treatment with Vitex agnus-castus essential oil the erythrocytes had a significant (p=0.029) and reversible increase in membrane fluidity in the deeper hydrophobic membrane regions, with no change (p>0.05) in fluidity near the membrane's hydrophilic surface. These results document increased fluidity of the human erythrocyte membrane by Vitex agnus-castus essential oil, and this action may be useful in patients with menopause-related hypertension and other cardiovascular conditions.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane
T1  - Vitex agnus-castus L.: Essential oil increases human erythrocyte membrane fluidity
EP  - 227
IS  - 3
SP  - 222
VL  - 31
DO  - 10.2478/v10011-011-0055-x
ER  - 

@article{
author = "Ajdzanović, Vladimir Z and Spasojević, Ivan and Pantelic, Jasmina B and Sosic-Jurjević, Branka T and Filipović, Branko R and Milošević, Verica Lj. and Severs, Walter",
year = "2012",
abstract = "Fluidnost membrane eritrocita uslovljava njihove reološke karakteristike, svojevrsne dinamičke kvalitete, izmenjene u hipertenziji i aterosklerozi. Rizik od navedenih i drugih kardiovaskularnih oboljenja se kod žena povećava sa starenjem. Tegobe koje prate menopauzu se obično ublažavaju supstitucionom hormonskom terapijom, koja može povećati rizik od pojave malignih oboljenja. Esencijalno ulje biljke Vitex agnus-castus L. sadrži različita organska jedinjenja (monoterpene, seskviterpene i terpenoide) i sve češće se koristi kao alternativno terapijsko sredstvo za simptome menopauze. Navedene komponente esencijalnog ulja mogu se ugraditi u ćelijske membrane i menjati njihovu fluidnost. Cilj studije bio je da se utvrde efekti esencijalnog ulja biljke Vitex agnus-castus na fluidnost membrane eritrocita po njenoj du bini. Korišćena je spektroskopija elektronske paramagnetne rezonance i masnokiselinske spinske probe (5-doksil stearinska i 12-doksil stearinska kiselina), čiji spektri za vise od fluidnosti membrane. Nakon tretmana eritrocita esencijalnim uljem biljke Vitex agnus-castus utvrđeno je značajno (p=0,029) i reverzibilno povećanje fluidnosti njihove membrane u dubljim, hidrofobnim regionima, dok u površinskim, hidrofilnim regionima eritrocitne membrane nisu utvrđene značajne promene (p>0,05). Ovi rezultati ukazuju na povećanje fluidnosti eritrocitne membrane nakon tretmana esencijalnim uljem biljke Vitex agnus-castus, što može biti koristan efekat pri tretmanu pacijentkinja sa hipertenzijom i drugim kardiovaskularnim oboljenjima u menopauzi., Erythrocyte membrane fluidity is related to their rheologic behavior, the dynamic quality of erythrocytes, which is tempted in hypertension and atherosclerosis. An increased risk of these and other cardiovascular diseases occurs in ageing women. Menopause-related conditions are often treated with hormone replacement therapy that may increase the risk of malignancies. Vitex agnus-castus L. essential oil contains various organic compounds (monoterpenes, sesquiterpenes and terpenoids), and is increasingly used as an alternative therapy for menopausal symptoms. These components of the oil may be incorporated into cell membranes, thereby changing the membrane fluidity. The aim of this study was to determine the effects of Vitex agnuscastus essential oil on human erythrocyte membrane fluidity at graded depths. We used Electron Paramagnetic Resonance spectroscopy and fatty acid spin probes (5-doxyl stearic acid and 12-doxyl stearic acid), whose spectra depend on membrane fluidity. After treatment with Vitex agnus-castus essential oil the erythrocytes had a significant (p=0.029) and reversible increase in membrane fluidity in the deeper hydrophobic membrane regions, with no change (p>0.05) in fluidity near the membrane's hydrophilic surface. These results document increased fluidity of the human erythrocyte membrane by Vitex agnus-castus essential oil, and this action may be useful in patients with menopause-related hypertension and other cardiovascular conditions.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane, Vitex agnus-castus L.: Essential oil increases human erythrocyte membrane fluidity",
pages = "227-222",
number = "3",
volume = "31",
doi = "10.2478/v10011-011-0055-x"
}

Ajdzanović, V. Z., Spasojević, I., Pantelic, J. B., Sosic-Jurjević, B. T., Filipović, B. R., Milošević, V. Lj.,& Severs, W.. (2012). Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 31(3), 222-227.
https://doi.org/10.2478/v10011-011-0055-x

Ajdzanović VZ, Spasojević I, Pantelic JB, Sosic-Jurjević BT, Filipović BR, Milošević VL, Severs W. Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane. in Journal of Medical Biochemistry. 2012;31(3):222-227.
doi:10.2478/v10011-011-0055-x .

Ajdzanović, Vladimir Z, Spasojević, Ivan, Pantelic, Jasmina B, Sosic-Jurjević, Branka T, Filipović, Branko R, Milošević, Verica Lj., Severs, Walter, "Esencijalno ulje biljke Vitex agnus-castus L. - povećava fluidnost eritrocitne membrane" in Journal of Medical Biochemistry, 31, no. 3 (2012):222-227,
https://doi.org/10.2478/v10011-011-0055-x . .

TY  - JOUR
AU  - Ajdzanović, Vladimir Z
AU  - Spasojević, Ivan
AU  - Sosic-Jurjević, Branka T
AU  - Filipović, Branko R
AU  - Trifunović, Svetlana L
AU  - Sekulic, Milka I
AU  - Milošević, Verica Lj.
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/482
AB  - A decrease of erythrocyte membrane fluidity can contribute to the pathophysiology of hypertension. Soy products, which are used as alternative therapeutics in some cardiovascular conditions, contain various isoflavones (genistein, daidzein, and their glucosides, genistin and daidzin), which can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of soy extract (which generally corresponds to the soy products of isoflavone composition) on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 12-DS), the spectra of which are dependent on membrane fluidity. After being treated with soy extract, erythrocytes showed a significant (P = 0.016) decrease of membrane fluidity near the hydrophilic surface, while there were no significant changes of fluidity in deeper hydrophobic membrane regions. These results suggest that soy products containing high levels of genistein and isoflavone glucosides may not be suitable for use in hypertension because they decrease erythrocyte membrane fluidity.
PB  - Springer, New York
T2  - Journal of Membrane Biology
T1  - The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study
EP  - 135
IS  - 3
SP  - 131
VL  - 239
DO  - 10.1007/s00232-010-9332-8
ER  - 

@article{
author = "Ajdzanović, Vladimir Z and Spasojević, Ivan and Sosic-Jurjević, Branka T and Filipović, Branko R and Trifunović, Svetlana L and Sekulic, Milka I and Milošević, Verica Lj.",
year = "2011",
abstract = "A decrease of erythrocyte membrane fluidity can contribute to the pathophysiology of hypertension. Soy products, which are used as alternative therapeutics in some cardiovascular conditions, contain various isoflavones (genistein, daidzein, and their glucosides, genistin and daidzin), which can incorporate cellular membrane and change its fluidity. The aim of this study was to examine the effects of soy extract (which generally corresponds to the soy products of isoflavone composition) on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 12-DS), the spectra of which are dependent on membrane fluidity. After being treated with soy extract, erythrocytes showed a significant (P = 0.016) decrease of membrane fluidity near the hydrophilic surface, while there were no significant changes of fluidity in deeper hydrophobic membrane regions. These results suggest that soy products containing high levels of genistein and isoflavone glucosides may not be suitable for use in hypertension because they decrease erythrocyte membrane fluidity.",
publisher = "Springer, New York",
journal = "Journal of Membrane Biology",
title = "The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study",
pages = "135-131",
number = "3",
volume = "239",
doi = "10.1007/s00232-010-9332-8"
}

Ajdzanović, V. Z., Spasojević, I., Sosic-Jurjević, B. T., Filipović, B. R., Trifunović, S. L., Sekulic, M. I.,& Milošević, V. Lj.. (2011). The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study. in Journal of Membrane Biology
Springer, New York., 239(3), 131-135.
https://doi.org/10.1007/s00232-010-9332-8

Ajdzanović VZ, Spasojević I, Sosic-Jurjević BT, Filipović BR, Trifunović SL, Sekulic MI, Milošević VL. The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study. in Journal of Membrane Biology. 2011;239(3):131-135.
doi:10.1007/s00232-010-9332-8 .

Ajdzanović, Vladimir Z, Spasojević, Ivan, Sosic-Jurjević, Branka T, Filipović, Branko R, Trifunović, Svetlana L, Sekulic, Milka I, Milošević, Verica Lj., "The Negative Effect of Soy Extract on Erythrocyte Membrane Fluidity: An Electron Paramagnetic Resonance Study" in Journal of Membrane Biology, 239, no. 3 (2011):131-135,
https://doi.org/10.1007/s00232-010-9332-8 . .

TY  - JOUR
AU  - Ajdzanović, Vladimir Z
AU  - Spasojević, Ivan
AU  - Filipović, Branko R
AU  - Sosic-Jurjević, Branka T
AU  - Sekulic, Milka I
AU  - Milošević, Verica Lj.
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/414
AB  - The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p  lt  0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p  lt  0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.
PB  - Natl Research Council Canada-N R C Research Press, Ottawa
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study
EP  - 500
IS  - 4
SP  - 497
VL  - 88
DO  - 10.1139/Y10-020
ER  - 

@article{
author = "Ajdzanović, Vladimir Z and Spasojević, Ivan and Filipović, Branko R and Sosic-Jurjević, Branka T and Sekulic, Milka I and Milošević, Verica Lj.",
year = "2010",
abstract = "The maintenance of erythrocyte membrane fluidity at the physiological level is an important factor affecting the ability of erythrocytes to pass through blood vessels of small luminal diameter. Genistein and daidzein, which are used as alternative therapeutics in cardiovascular conditions, can be incorporated into the cell membrane and change its fluidity. The aim of this study was to examine the effects of genistein and daidzein on erythrocyte membrane fluidity at graded depths. We used electron paramagnetic resonance (EPR) spectroscopy and fatty acid spin probes (5-DS and 12-DS) where EPR spectra were dependent on fluidity. The results showed that genistein significantly (p  lt  0.05) decreased erythrocyte membrane fluidity near the hydrophilic surface, while daidzein significantly (p  lt  0.05) increased the same parameter in deeper regions of the membrane. These data suggest that the deep fluidizing effects of daidzein on erythrocyte membranes make it a better therapeutic choice than genistein in some cardiovascular conditions.",
publisher = "Natl Research Council Canada-N R C Research Press, Ottawa",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study",
pages = "500-497",
number = "4",
volume = "88",
doi = "10.1139/Y10-020"
}

Ajdzanović, V. Z., Spasojević, I., Filipović, B. R., Sosic-Jurjević, B. T., Sekulic, M. I.,& Milošević, V. Lj.. (2010). Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology
Natl Research Council Canada-N R C Research Press, Ottawa., 88(4), 497-500.
https://doi.org/10.1139/Y10-020

Ajdzanović VZ, Spasojević I, Filipović BR, Sosic-Jurjević BT, Sekulic MI, Milošević VL. Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study. in Canadian Journal of Physiology and Pharmacology. 2010;88(4):497-500.
doi:10.1139/Y10-020 .

Ajdzanović, Vladimir Z, Spasojević, Ivan, Filipović, Branko R, Sosic-Jurjević, Branka T, Sekulic, Milka I, Milošević, Verica Lj., "Effects of genistein and daidzein on erythrocyte membrane fluidity: an electron paramagnetic resonance study" in Canadian Journal of Physiology and Pharmacology, 88, no. 4 (2010):497-500,
https://doi.org/10.1139/Y10-020 . .