TY  - CONF
AU  - Popović, Nikolina
AU  - Prodanović, Olivera
AU  - Gadjanski, Ivana
AU  - Cvetković, Danijela
AU  - Živanović, Marko
AU  - Pavlović, Vladimir
AU  - Filipović, Nenad D.
AU  - Prodanović, Radivoje
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3123
AB  - Carboxymethylcellulose (CMC) is water-soluble cellulose ether which is used in food and cosmetics industry. It also
has big potential for use in pharmaceutical products due to its high biocompatibility, biodegradibility, low immunogenicity and
low price. Crosslinked CMC can absorb large amounts of water and swell to form hydrogels with great physical properties.
The need for new biomaterials and hydrogels is growing daily, due to their use in tissue engineering, drug delivery and cell
and enzyme immobilization studies. In this study we modified CMC, in order to get a crosslinkable polymer that can make
hydrogels by chemical and enzymatic means. After periodate oxidation of CMC we obtained CMC with different degrees of
oxidation: 2.5, 5, 10, 15 and 20 mol%. Further modification using reductive amination in the presence of different phenolic
compounds like tyramine, was done. This modification of CMC was confirmed by UV–VIS and FT-IR spectroscopy, while
concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid–base titration. All CMCtyramines
were able to form hydrogels after cross-linking with horse radish peroxidase (HRP) and hydrogen peroxide. CMC
derivatives have been successfully electrospun and crosslinked afterwards. Due to the introduction of amino groups and
decrease in molecular weight, they were significantly more soluble in water up to 30 % (w/w) compared to native
polysaccharides and their electrospinability also improved. We aim to make nanofibers using tyramine-polysaccharides that
will be more stable in cell culture media after cross-linking covalently and with calcium/barium ions. Diameter of nanofibers
was determined by scanning electron microscopy (SEM). Cross-linked nanofibers that we obtained will be used for tissue
engineering of blood vessels.
PB  - West University of Timișoara
C3  - The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar
T1  - MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING
IS  - 2
SP  - S4_P10
VL  - 26
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3123
ER  - 

@conference{
author = "Popović, Nikolina and Prodanović, Olivera and Gadjanski, Ivana and Cvetković, Danijela and Živanović, Marko and Pavlović, Vladimir and Filipović, Nenad D. and Prodanović, Radivoje",
year = "2017",
abstract = "Carboxymethylcellulose (CMC) is water-soluble cellulose ether which is used in food and cosmetics industry. It also
has big potential for use in pharmaceutical products due to its high biocompatibility, biodegradibility, low immunogenicity and
low price. Crosslinked CMC can absorb large amounts of water and swell to form hydrogels with great physical properties.
The need for new biomaterials and hydrogels is growing daily, due to their use in tissue engineering, drug delivery and cell
and enzyme immobilization studies. In this study we modified CMC, in order to get a crosslinkable polymer that can make
hydrogels by chemical and enzymatic means. After periodate oxidation of CMC we obtained CMC with different degrees of
oxidation: 2.5, 5, 10, 15 and 20 mol%. Further modification using reductive amination in the presence of different phenolic
compounds like tyramine, was done. This modification of CMC was confirmed by UV–VIS and FT-IR spectroscopy, while
concentration of phenol and ionizable groups was determined using absorbance at 275 nm and acid–base titration. All CMCtyramines
were able to form hydrogels after cross-linking with horse radish peroxidase (HRP) and hydrogen peroxide. CMC
derivatives have been successfully electrospun and crosslinked afterwards. Due to the introduction of amino groups and
decrease in molecular weight, they were significantly more soluble in water up to 30 % (w/w) compared to native
polysaccharides and their electrospinability also improved. We aim to make nanofibers using tyramine-polysaccharides that
will be more stable in cell culture media after cross-linking covalently and with calcium/barium ions. Diameter of nanofibers
was determined by scanning electron microscopy (SEM). Cross-linked nanofibers that we obtained will be used for tissue
engineering of blood vessels.",
publisher = "West University of Timișoara",
journal = "The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar",
title = "MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING",
number = "2",
pages = "S4_P10",
volume = "26",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3123"
}

Popović, N., Prodanović, O., Gadjanski, I., Cvetković, D., Živanović, M., Pavlović, V., Filipović, N. D.,& Prodanović, R.. (2017). MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING. in The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar
West University of Timișoara., 26(2), S4_P10.
https://hdl.handle.net/21.15107/rcub_rimsi_3123

Popović N, Prodanović O, Gadjanski I, Cvetković D, Živanović M, Pavlović V, Filipović ND, Prodanović R. MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING. in The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar. 2017;26(2):S4_P10.
https://hdl.handle.net/21.15107/rcub_rimsi_3123 .

Popović, Nikolina, Prodanović, Olivera, Gadjanski, Ivana, Cvetković, Danijela, Živanović, Marko, Pavlović, Vladimir, Filipović, Nenad D., Prodanović, Radivoje, "MODIFICATION OF CARBOXYMETHYLCELLULOSE WITH PHENOLS FOR PEROXIDASE INDUCED HYDROGELS FORMATION AND ELECTROSPINNING" in The Annual International Conference Romanian Society for Biochemistry & Molecular Biology, 8 - 9. June, Temišvar, 26, no. 2 (2017):S4_P10,
https://hdl.handle.net/21.15107/rcub_rimsi_3123 .