TY  - CONF
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Spasojević, Dragica
AU  - Stojanović, Željko
AU  - Radotić, Ksenija
AU  - Marinković, Emilija
AU  - Prodanović, Radivoje
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1803
AB  - Wastewater treatment is a current and important environmental issue. Phenolic compounds most of which are
toxic and many even carcinogens, found in various polluted waters are non-biodegradable and present a serious
health hazard. Enzymatic treatment, using peroxidase and H2O2, provides a highly selective and efficient alternative
to current phenol removal methods, with low energy requirements and minimal environmental impact.
Soybean hull peroxidase (SHP) isolated from soybean hulls, which are inexpensive agricultural waste products,
offers a cheap source of crude enzyme available for various applications. In this study we used macroporous
glycidyl methacrylate based copolymers with various surface characteristics and mean pore size diameter ranging
from 40-200 nm as carriers and compared two different immobilization methods for SHP – glutaraldehyde
and periodate. Our results demonstrate that SHP immobilization with both methods is influenced by the pore
size of the carrier matrix with both the specific activity of the immobilized enzyme and immobilization yeald
increased with pore size. Glutaraldehyde immobilization method proved to be substantially better than periodate
with the highest specific activities obtained 22.8 U/g and 3.4 U/g of carrier respectively. Thermal stability
at 85°C and stability in 80% dioxane solution indicate a stabilization of the immobilized enzyme compared to
the free form. Although having the same pH optimum, immobilized enzyme operates over a broader pH range.
Obtained biocatalyst shows an increased specific activity, higher thermal and organic solvent stability and operates
over wider pH range and it’s suitable for wastewater treatment.
PB  - MTG - Topgraf Velika Gorica
C3  - 4th Croatian Botanical Symposium with international participation, Split 2013
T1  - Different immobilization methods of soybean hull peroxidase on macroporous glycidyl methacrylate copolymers
SP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1803
ER  - 

@conference{
author = "Prokopijević, Miloš and Prodanović, Olivera and Spasojević, Dragica and Stojanović, Željko and Radotić, Ksenija and Marinković, Emilija and Prodanović, Radivoje",
year = "2013",
abstract = "Wastewater treatment is a current and important environmental issue. Phenolic compounds most of which are
toxic and many even carcinogens, found in various polluted waters are non-biodegradable and present a serious
health hazard. Enzymatic treatment, using peroxidase and H2O2, provides a highly selective and efficient alternative
to current phenol removal methods, with low energy requirements and minimal environmental impact.
Soybean hull peroxidase (SHP) isolated from soybean hulls, which are inexpensive agricultural waste products,
offers a cheap source of crude enzyme available for various applications. In this study we used macroporous
glycidyl methacrylate based copolymers with various surface characteristics and mean pore size diameter ranging
from 40-200 nm as carriers and compared two different immobilization methods for SHP – glutaraldehyde
and periodate. Our results demonstrate that SHP immobilization with both methods is influenced by the pore
size of the carrier matrix with both the specific activity of the immobilized enzyme and immobilization yeald
increased with pore size. Glutaraldehyde immobilization method proved to be substantially better than periodate
with the highest specific activities obtained 22.8 U/g and 3.4 U/g of carrier respectively. Thermal stability
at 85°C and stability in 80% dioxane solution indicate a stabilization of the immobilized enzyme compared to
the free form. Although having the same pH optimum, immobilized enzyme operates over a broader pH range.
Obtained biocatalyst shows an increased specific activity, higher thermal and organic solvent stability and operates
over wider pH range and it’s suitable for wastewater treatment.",
publisher = "MTG - Topgraf Velika Gorica",
journal = "4th Croatian Botanical Symposium with international participation, Split 2013",
title = "Different immobilization methods of soybean hull peroxidase on macroporous glycidyl methacrylate copolymers",
pages = "90",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1803"
}

Prokopijević, M., Prodanović, O., Spasojević, D., Stojanović, Ž., Radotić, K., Marinković, E.,& Prodanović, R.. (2013). Different immobilization methods of soybean hull peroxidase on macroporous glycidyl methacrylate copolymers. in 4th Croatian Botanical Symposium with international participation, Split 2013
MTG - Topgraf Velika Gorica., 90.
https://hdl.handle.net/21.15107/rcub_rimsi_1803

Prokopijević M, Prodanović O, Spasojević D, Stojanović Ž, Radotić K, Marinković E, Prodanović R. Different immobilization methods of soybean hull peroxidase on macroporous glycidyl methacrylate copolymers. in 4th Croatian Botanical Symposium with international participation, Split 2013. 2013;:90.
https://hdl.handle.net/21.15107/rcub_rimsi_1803 .

Prokopijević, Miloš, Prodanović, Olivera, Spasojević, Dragica, Stojanović, Željko, Radotić, Ksenija, Marinković, Emilija, Prodanović, Radivoje, "Different immobilization methods of soybean hull peroxidase on macroporous glycidyl methacrylate copolymers" in 4th Croatian Botanical Symposium with international participation, Split 2013 (2013):90,
https://hdl.handle.net/21.15107/rcub_rimsi_1803 .