TY  - JOUR
AU  - Simonović, Mladen
AU  - Ostojic, Sanja
AU  - Micic, Darko
AU  - Bisercic-Savić, Marjetka
AU  - Mix, Thorsten
AU  - Glumac, Miodrag
AU  - Pejin, Boris
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1537
AB  - This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - A novel and effective natural product-based immunodetection tool for TNT-like compounds
EP  - 861
IS  - 3
SP  - 857
VL  - 36
DO  - 10.1080/14786419.2020.1806269
ER  - 

@article{
author = "Simonović, Mladen and Ostojic, Sanja and Micic, Darko and Bisercic-Savić, Marjetka and Mix, Thorsten and Glumac, Miodrag and Pejin, Boris",
year = "2022",
abstract = "This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "A novel and effective natural product-based immunodetection tool for TNT-like compounds",
pages = "861-857",
number = "3",
volume = "36",
doi = "10.1080/14786419.2020.1806269"
}

Simonović, M., Ostojic, S., Micic, D., Bisercic-Savić, M., Mix, T., Glumac, M.,& Pejin, B.. (2022). A novel and effective natural product-based immunodetection tool for TNT-like compounds. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 36(3), 857-861.
https://doi.org/10.1080/14786419.2020.1806269

Simonović M, Ostojic S, Micic D, Bisercic-Savić M, Mix T, Glumac M, Pejin B. A novel and effective natural product-based immunodetection tool for TNT-like compounds. in Natural Product Research. 2022;36(3):857-861.
doi:10.1080/14786419.2020.1806269 .

Simonović, Mladen, Ostojic, Sanja, Micic, Darko, Bisercic-Savić, Marjetka, Mix, Thorsten, Glumac, Miodrag, Pejin, Boris, "A novel and effective natural product-based immunodetection tool for TNT-like compounds" in Natural Product Research, 36, no. 3 (2022):857-861,
https://doi.org/10.1080/14786419.2020.1806269 . .

TY  - JOUR
AU  - Simonović, Mladen
AU  - Simonović, Branislav R.
AU  - Ostojic, Sanja
AU  - Pezo, Lato
AU  - Micic, Darko
AU  - Stanisavljević, Nemanja
AU  - Pejin, Boris
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1219
AB  - This study aimed to estimate the quality (in terms of standard chemical parameters, total antioxidative activity /AO/ and thermal properties) of the fresh strawberry (Fragaria xananassa L. Duch.), raspberry (Rubus idaeus L.) and blackberry (Rubus fruticosusL.) samples grown in the Western Serbia. The examined blackberry variety was found to exhibit the most potent AO (DPPH /2,2-diphenyl-1-picrylhydrazyl/ assay, EC50 4.05 +/- 0.22 mu g and ARP 0.25 +/- 0.03 mu g(-1); Hydroxo-Perhydroxyl Mercury(II) Complex /HPMC/ assay, 0.072 +/- 0.007%/mu L), due to the enriched contents of both total phenolics and anthocyanins. It was actually followed by the raspberry and strawberry samples. Unlike the aforementioned trend for AO, thermal behaviour of all samples was similar. This is first time ever that a DC polarographic HPMC assay has been applied for the characterisation of the fresh berry fruits. Further studies aiming to screen total AO of the soft fruit during growth, storage and processing might be well facilitated by the application of this low cost, rapid, easy-to-handle and reliable electrochemical assay.
PB  - Elsevier, Amsterdam
T2  - Scientia Horticulturae
T1  - A contribution to the estimation of berry fruits quality
VL  - 258
DO  - 10.1016/j.scienta.2019.108776
ER  - 

@article{
author = "Simonović, Mladen and Simonović, Branislav R. and Ostojic, Sanja and Pezo, Lato and Micic, Darko and Stanisavljević, Nemanja and Pejin, Boris",
year = "2019",
abstract = "This study aimed to estimate the quality (in terms of standard chemical parameters, total antioxidative activity /AO/ and thermal properties) of the fresh strawberry (Fragaria xananassa L. Duch.), raspberry (Rubus idaeus L.) and blackberry (Rubus fruticosusL.) samples grown in the Western Serbia. The examined blackberry variety was found to exhibit the most potent AO (DPPH /2,2-diphenyl-1-picrylhydrazyl/ assay, EC50 4.05 +/- 0.22 mu g and ARP 0.25 +/- 0.03 mu g(-1); Hydroxo-Perhydroxyl Mercury(II) Complex /HPMC/ assay, 0.072 +/- 0.007%/mu L), due to the enriched contents of both total phenolics and anthocyanins. It was actually followed by the raspberry and strawberry samples. Unlike the aforementioned trend for AO, thermal behaviour of all samples was similar. This is first time ever that a DC polarographic HPMC assay has been applied for the characterisation of the fresh berry fruits. Further studies aiming to screen total AO of the soft fruit during growth, storage and processing might be well facilitated by the application of this low cost, rapid, easy-to-handle and reliable electrochemical assay.",
publisher = "Elsevier, Amsterdam",
journal = "Scientia Horticulturae",
title = "A contribution to the estimation of berry fruits quality",
volume = "258",
doi = "10.1016/j.scienta.2019.108776"
}

Simonović, M., Simonović, B. R., Ostojic, S., Pezo, L., Micic, D., Stanisavljević, N.,& Pejin, B.. (2019). A contribution to the estimation of berry fruits quality. in Scientia Horticulturae
Elsevier, Amsterdam., 258.
https://doi.org/10.1016/j.scienta.2019.108776

Simonović M, Simonović BR, Ostojic S, Pezo L, Micic D, Stanisavljević N, Pejin B. A contribution to the estimation of berry fruits quality. in Scientia Horticulturae. 2019;258.
doi:10.1016/j.scienta.2019.108776 .

Simonović, Mladen, Simonović, Branislav R., Ostojic, Sanja, Pezo, Lato, Micic, Darko, Stanisavljević, Nemanja, Pejin, Boris, "A contribution to the estimation of berry fruits quality" in Scientia Horticulturae, 258 (2019),
https://doi.org/10.1016/j.scienta.2019.108776 . .