TY  - JOUR
AU  - Simonović, Mladen
AU  - Mix, Thorsten
AU  - Glumac, Miodrag
AU  - Pejin, Boris
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1547
AB  - A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of beta-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-beta-lactamase. This bifunctional immunoreagent tool was further convincingly demonstrated for being capable to directly detect trinitrophenol-tris(hydroxymethyl)aminomethane (TNP-Tris), a less toxic TNP derivative of choice compared to TNP itself, with competitive sensitivity (50 +/- 2 fmol or 175 +/- 6 pg/mL).
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - 3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive
EP  - 1320
IS  - 5
SP  - 1317
VL  - 36
DO  - 10.1080/14786419.2020.1860977
ER  - 

@article{
author = "Simonović, Mladen and Mix, Thorsten and Glumac, Miodrag and Pejin, Boris",
year = "2022",
abstract = "A new protein detector of 2,4,6-trinitrophenol (TNP, picric acid) is concisely reported herein for the first time. In brief, the gene of a specific scFv fragment, namely 3.5, was fused separately with the gene of beta-lactamase and, subsequently, expressed in the periplasmic space of Escherichia coli, affording in such a way the recombinant fusion protein 3.5-scFv-beta-lactamase. This bifunctional immunoreagent tool was further convincingly demonstrated for being capable to directly detect trinitrophenol-tris(hydroxymethyl)aminomethane (TNP-Tris), a less toxic TNP derivative of choice compared to TNP itself, with competitive sensitivity (50 +/- 2 fmol or 175 +/- 6 pg/mL).",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive",
pages = "1320-1317",
number = "5",
volume = "36",
doi = "10.1080/14786419.2020.1860977"
}

Simonović, M., Mix, T., Glumac, M.,& Pejin, B.. (2022). 3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 36(5), 1317-1320.
https://doi.org/10.1080/14786419.2020.1860977

Simonović M, Mix T, Glumac M, Pejin B. 3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive. in Natural Product Research. 2022;36(5):1317-1320.
doi:10.1080/14786419.2020.1860977 .

Simonović, Mladen, Mix, Thorsten, Glumac, Miodrag, Pejin, Boris, "3.5-scFv-beta-lactamase, a new protein detector of picric acid, the highly nitrated organic explosive" in Natural Product Research, 36, no. 5 (2022):1317-1320,
https://doi.org/10.1080/14786419.2020.1860977 . .

TY  - JOUR
AU  - Simonović, Mladen
AU  - Ostojic, Sanja
AU  - Micic, Darko
AU  - Bisercic-Savić, Marjetka
AU  - Mix, Thorsten
AU  - Glumac, Miodrag
AU  - Pejin, Boris
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1537
AB  - This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .
PB  - Taylor & Francis Ltd, Abingdon
T2  - Natural Product Research
T1  - A novel and effective natural product-based immunodetection tool for TNT-like compounds
EP  - 861
IS  - 3
SP  - 857
VL  - 36
DO  - 10.1080/14786419.2020.1806269
ER  - 

@article{
author = "Simonović, Mladen and Ostojic, Sanja and Micic, Darko and Bisercic-Savić, Marjetka and Mix, Thorsten and Glumac, Miodrag and Pejin, Boris",
year = "2022",
abstract = "This study aimed to develop a fast and reliable protocol for Trinitrophenol-Tris(hydroxymethyl)aminomethane (TNP-Tris) detection applying a beta-lactamase-fusion protein of choice, the natural product-based immunoreagent tool of competitive sensitivity developed herein for the first time. Since the fusion protein 11B3-scFv-beta-lactamase is constructed from a scFv-antibody (11B3) linked to an enzyme (beta-lactamase), the step related to the use of secondary antibody in the enzyme-linked immunosorbent assay (ELISA) is completely omitted. Indeed, this fusion protein itself serves both as binding mean of the antigen model and detecting agent, due to the presence of the naturally occurring enzyme. In such a way, it actually affords the one-step TNP-Tris detection reaching a promising LOD value of 45 +/- 2 fmol or 157 +/- 6 pg/mL. Taken all together, the current protocol does represent much cheaper and significantly less-time consuming alternative compared both to the recombinant antibodies and recombinant phages, previously designed means in our labs for the same purpose. [GRAPHICS] .",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Natural Product Research",
title = "A novel and effective natural product-based immunodetection tool for TNT-like compounds",
pages = "861-857",
number = "3",
volume = "36",
doi = "10.1080/14786419.2020.1806269"
}

Simonović, M., Ostojic, S., Micic, D., Bisercic-Savić, M., Mix, T., Glumac, M.,& Pejin, B.. (2022). A novel and effective natural product-based immunodetection tool for TNT-like compounds. in Natural Product Research
Taylor & Francis Ltd, Abingdon., 36(3), 857-861.
https://doi.org/10.1080/14786419.2020.1806269

Simonović M, Ostojic S, Micic D, Bisercic-Savić M, Mix T, Glumac M, Pejin B. A novel and effective natural product-based immunodetection tool for TNT-like compounds. in Natural Product Research. 2022;36(3):857-861.
doi:10.1080/14786419.2020.1806269 .

Simonović, Mladen, Ostojic, Sanja, Micic, Darko, Bisercic-Savić, Marjetka, Mix, Thorsten, Glumac, Miodrag, Pejin, Boris, "A novel and effective natural product-based immunodetection tool for TNT-like compounds" in Natural Product Research, 36, no. 3 (2022):857-861,
https://doi.org/10.1080/14786419.2020.1806269 . .