@conference{
author = "Marković, Sanja and Popović, Tatjana and Mitrović, Petar and Iličić, Renata and Jelušić, Aleksandra",
year = "2022",
abstract = "In August 2021, symptoms of soft rot appeared on cabbage
in Futog (Bačka, Vojvodina) locality known for traditional
cabbage cultivation in Serbia. Symptoms appeared in the
form of sunken and soft lesions on outer head leaves, while
interior tissue was macerated, with cream to black
discoloration. A strong, specific odor followed the
breakdown of the affected tissue. From the 1 ha field, a
total of 5 heads were collected for the isolation of causing
pathogen. Margins of healthy and dead tissues were
macerated and plated on Crystal Violet Pectate medium.
Five creamy-white colonies forming pits in the medium
were purified. These bacterial isolates were positive for
pectinolytic activity on cabbage heads and potato slices.
They were all facultative anaerobic, Gram-negative,
catalase-positive, and oxidase-negative; unable to produce
diffusible fluorescent pigments. Multilocus sequence
typing of cabbage isolates was performed by sequencing
of four housekeeping genes (proA, dnaX, icdA, mdh).
Based on the nucleotide BLAST (National Center for
Biotechnology Information, NCBI) analysis of the
sequences of genes icdA and mdh, Serbian cabbage isolates
were 100% identical with Pectobacterium carotovorum
subsp. odoriferum strain BC S7. Based on the dnaX gene,
identity with the same strain was 99.79% and 99.57%
based on the proA gene. Serbian cabbage isolates also
showed 100% identity with other NCBI deposited P.
odoriferum strains CFBP1878 and ATCC 25272 based on
the icdA gene, as well as ICMP 11533 based on mdh gene.
Therefore, this first report of P. odoriferum indicates a
wider range of Pectobacterium spp. recently described in
Serbia.",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference on Plant Pathogenic Bacteria",
title = "First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia",
pages = "123",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1959"
}