TY  - JOUR
AU  - Marjanović, Žaklina
AU  - Nawaz, Ali
AU  - Stevanović, Katarina
AU  - Saljnikov, Elmira
AU  - Macek, Irena
AU  - Oehl, Fritz
AU  - Wubet, Tesfaye
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1370
AB  - Balkan lowlands bordering with the Pannonia region are inhabited by diverse riparian forests that support production of different truffle species, predominantly the most prized white truffle of Piedmont (Tuber magnatumPico), but also other commercial species (T.macrosporumVitt.,T.aestivumVitt.). Surprisingly, little is known about the native root-associated mycobiome (RAM) of these lowland truffle-producing forests. Therefore, in this study we aim at exploring and comparing the RAMs of three different truffle-producing forests from Kolubara river plane in Serbia. Molecular methods based on next generation sequencing (NGS) were used to evaluate the diversity of root-associated fungal communities and to elucidate the influence of environmental factors on their differentiation. To our knowledge, this is the first study from such habitats with a particular focus on comparative analysis of the RAM in different truffle-producing habitats using a high-throughput sequencing approach. Our results indicated that the alpha diversity of investigated fungal communities was not significantly different between different truffle-producing forests and within a specific forest type, while the seasonal differences in the alpha diversity were only observed in the white truffle-producing forests. Taxonomic profiling at phylum level indicated the dominance of fungal OTUs belonging to phylum Ascomycota and Basidiomycota, with very minor presence of other phyla. Distinct community structures of root-associated mycobiomes were observed for white, mixed, and black truffle-producing forests. The core mycobiome analysis indicated a fair share of fungal genera present exclusively in white and black truffle-producing forest, while the core genera of mixed truffle-producing forests were shared with both white and black truffle-producing forests. The majority of detected fungal OTUs in all three forest types were symbiotrophs, with ectomycorrhizal fungi being a dominant functional guild. Apart from assumed vegetation factor, differentiation of fungal communities was driven by factors connected to the distance from the river and exposure to fluvial activities, soil age, structure, and pH. Overall, Pannonian riparian forests appear to host diverse root-associated fungal communities that are strongly shaped by variation in soil conditions.
PB  - MDPI, Basel
T2  - Microorganisms
T1  - Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests
IS  - 9
VL  - 8
DO  - 10.3390/microorganisms8091331
ER  - 

@article{
author = "Marjanović, Žaklina and Nawaz, Ali and Stevanović, Katarina and Saljnikov, Elmira and Macek, Irena and Oehl, Fritz and Wubet, Tesfaye",
year = "2020",
abstract = "Balkan lowlands bordering with the Pannonia region are inhabited by diverse riparian forests that support production of different truffle species, predominantly the most prized white truffle of Piedmont (Tuber magnatumPico), but also other commercial species (T.macrosporumVitt.,T.aestivumVitt.). Surprisingly, little is known about the native root-associated mycobiome (RAM) of these lowland truffle-producing forests. Therefore, in this study we aim at exploring and comparing the RAMs of three different truffle-producing forests from Kolubara river plane in Serbia. Molecular methods based on next generation sequencing (NGS) were used to evaluate the diversity of root-associated fungal communities and to elucidate the influence of environmental factors on their differentiation. To our knowledge, this is the first study from such habitats with a particular focus on comparative analysis of the RAM in different truffle-producing habitats using a high-throughput sequencing approach. Our results indicated that the alpha diversity of investigated fungal communities was not significantly different between different truffle-producing forests and within a specific forest type, while the seasonal differences in the alpha diversity were only observed in the white truffle-producing forests. Taxonomic profiling at phylum level indicated the dominance of fungal OTUs belonging to phylum Ascomycota and Basidiomycota, with very minor presence of other phyla. Distinct community structures of root-associated mycobiomes were observed for white, mixed, and black truffle-producing forests. The core mycobiome analysis indicated a fair share of fungal genera present exclusively in white and black truffle-producing forest, while the core genera of mixed truffle-producing forests were shared with both white and black truffle-producing forests. The majority of detected fungal OTUs in all three forest types were symbiotrophs, with ectomycorrhizal fungi being a dominant functional guild. Apart from assumed vegetation factor, differentiation of fungal communities was driven by factors connected to the distance from the river and exposure to fluvial activities, soil age, structure, and pH. Overall, Pannonian riparian forests appear to host diverse root-associated fungal communities that are strongly shaped by variation in soil conditions.",
publisher = "MDPI, Basel",
journal = "Microorganisms",
title = "Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests",
number = "9",
volume = "8",
doi = "10.3390/microorganisms8091331"
}

Marjanović, Ž., Nawaz, A., Stevanović, K., Saljnikov, E., Macek, I., Oehl, F.,& Wubet, T.. (2020). Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests. in Microorganisms
MDPI, Basel., 8(9).
https://doi.org/10.3390/microorganisms8091331

Marjanović Ž, Nawaz A, Stevanović K, Saljnikov E, Macek I, Oehl F, Wubet T. Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests. in Microorganisms. 2020;8(9).
doi:10.3390/microorganisms8091331 .

Marjanović, Žaklina, Nawaz, Ali, Stevanović, Katarina, Saljnikov, Elmira, Macek, Irena, Oehl, Fritz, Wubet, Tesfaye, "Root-Associated Mycobiome Differentiate between Habitats Supporting Production of Different Truffle Species in Serbian Riparian Forests" in Microorganisms, 8, no. 9 (2020),
https://doi.org/10.3390/microorganisms8091331 . .

TY  - JOUR
AU  - Marjanović, Žaklina
AU  - Glisic, A.
AU  - Mutavdžić, Dragosav
AU  - Saljnikov, Elmira
AU  - Bragato, Gilberto
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/855
AB  - The production of Tuber magnatum Pico, the most prized and ecologically the most complex, constantly declines in natural habitats, while the success in plantation lacks. Contrary to the data from its habitats in Mediterranean sites, in Serbia this truffle colonizes typical developed alluvial forests, implying that dynamics of soil parameters and nutrient availability, rather than presence of specific ectomycorrhizal (EMC) plant host species or climate, might be crucial for supporting truffle life cycle. In order to reveal specific relationship generating soil microenvironments in productive and unproductive sites, soil water content (SWC) and temperature (T) were measured periodically in two depths, in a typical T. magnatum habitat in Western Serbia and compared to the atmospheric parameters (precipitation, air temperature). In three key time-points (spring soil water saturation, summer drought and autumn ascocarp production) soil was sampled through the profile and analyzed for nutrients that might be important for plant/fungal growth and ectomycorrhiza establishment. Results revealed that specific dynamics of soil water, aeration, available P and N, and possibly vegetation phenology, might be determining productivity of the microsites within the productive area. The hypothesis on soil water and nutrient availability seasonal dynamics, which might be controlling truffle establishment and life cycle completion in investigated area, was proposed.
PB  - Elsevier Science Bv, Amsterdam
T2  - Applied Soil Ecology
T1  - Ecosystems supporting Tuber magnatum Pico production in Serbia experience specific soil environment seasonality that may facilitate truffle lifecycle completion
EP  - 190
SP  - 179
VL  - 95
DO  - 10.1016/j.apsoil.2015.05.007
ER  - 

@article{
author = "Marjanović, Žaklina and Glisic, A. and Mutavdžić, Dragosav and Saljnikov, Elmira and Bragato, Gilberto",
year = "2015",
abstract = "The production of Tuber magnatum Pico, the most prized and ecologically the most complex, constantly declines in natural habitats, while the success in plantation lacks. Contrary to the data from its habitats in Mediterranean sites, in Serbia this truffle colonizes typical developed alluvial forests, implying that dynamics of soil parameters and nutrient availability, rather than presence of specific ectomycorrhizal (EMC) plant host species or climate, might be crucial for supporting truffle life cycle. In order to reveal specific relationship generating soil microenvironments in productive and unproductive sites, soil water content (SWC) and temperature (T) were measured periodically in two depths, in a typical T. magnatum habitat in Western Serbia and compared to the atmospheric parameters (precipitation, air temperature). In three key time-points (spring soil water saturation, summer drought and autumn ascocarp production) soil was sampled through the profile and analyzed for nutrients that might be important for plant/fungal growth and ectomycorrhiza establishment. Results revealed that specific dynamics of soil water, aeration, available P and N, and possibly vegetation phenology, might be determining productivity of the microsites within the productive area. The hypothesis on soil water and nutrient availability seasonal dynamics, which might be controlling truffle establishment and life cycle completion in investigated area, was proposed.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Applied Soil Ecology",
title = "Ecosystems supporting Tuber magnatum Pico production in Serbia experience specific soil environment seasonality that may facilitate truffle lifecycle completion",
pages = "190-179",
volume = "95",
doi = "10.1016/j.apsoil.2015.05.007"
}

Marjanović, Ž., Glisic, A., Mutavdžić, D., Saljnikov, E.,& Bragato, G.. (2015). Ecosystems supporting Tuber magnatum Pico production in Serbia experience specific soil environment seasonality that may facilitate truffle lifecycle completion. in Applied Soil Ecology
Elsevier Science Bv, Amsterdam., 95, 179-190.
https://doi.org/10.1016/j.apsoil.2015.05.007

Marjanović Ž, Glisic A, Mutavdžić D, Saljnikov E, Bragato G. Ecosystems supporting Tuber magnatum Pico production in Serbia experience specific soil environment seasonality that may facilitate truffle lifecycle completion. in Applied Soil Ecology. 2015;95:179-190.
doi:10.1016/j.apsoil.2015.05.007 .

Marjanović, Žaklina, Glisic, A., Mutavdžić, Dragosav, Saljnikov, Elmira, Bragato, Gilberto, "Ecosystems supporting Tuber magnatum Pico production in Serbia experience specific soil environment seasonality that may facilitate truffle lifecycle completion" in Applied Soil Ecology, 95 (2015):179-190,
https://doi.org/10.1016/j.apsoil.2015.05.007 . .

TY  - JOUR
AU  - Pavić, Aleksandar B
AU  - Stanković, Slaviša
AU  - Saljnikov, Elmira
AU  - Krueger, Dirk
AU  - Buscot, Francois
AU  - Tarkka, Mika
AU  - Marjanović, Žaklina
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/684
AB  - To test the hypothesis that truffle-associated bacteria may improve truffle nutrition, we isolated bacteria from white truffle ascocarps and tested Actinobacteria for their ability to solubilise phosphate and iron, nutrients that have limited availability in white truffle grounds. Two isolates with sequence similarities to Curtobacterium flaccumfaciens and Rhodococcus sp. were characterized in detail. Both solubilised Ca-3(PO4)(2) in a way that was dependent on the nitrogen and carbon sources present. Neither strain broke down phytate, but both produced chelating compounds, performed ammonification, and broke down beta-glucan. Additionally, C. flaccumfaciens decomposed chitin, pectin, lipids and proteins, while Rhodococcus sp. exhibited urease activity. Three potentially fungicolous fungi were isolated from diseased white truffle ascocarps and bioassayed against the isolated Actinobacteria. The Rhodococcus isolate inhibited Verticillium leptobactrum, neither bacterium affected Clonostachys rosea, while both isolates promoted growth of Trichoderma sp. The results suggest that Actinobacteria might be involved in improving truffle nutrition, ascocarp degradation and establishing relationships with other soil fungi.
PB  - Elsevier Sci Ltd, Oxford
T2  - Fungal Ecology
T1  - Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi
EP  - 538
IS  - 6
SP  - 527
VL  - 6
DO  - 10.1016/j.funeco.2013.05.006
ER  - 

@article{
author = "Pavić, Aleksandar B and Stanković, Slaviša and Saljnikov, Elmira and Krueger, Dirk and Buscot, Francois and Tarkka, Mika and Marjanović, Žaklina",
year = "2013",
abstract = "To test the hypothesis that truffle-associated bacteria may improve truffle nutrition, we isolated bacteria from white truffle ascocarps and tested Actinobacteria for their ability to solubilise phosphate and iron, nutrients that have limited availability in white truffle grounds. Two isolates with sequence similarities to Curtobacterium flaccumfaciens and Rhodococcus sp. were characterized in detail. Both solubilised Ca-3(PO4)(2) in a way that was dependent on the nitrogen and carbon sources present. Neither strain broke down phytate, but both produced chelating compounds, performed ammonification, and broke down beta-glucan. Additionally, C. flaccumfaciens decomposed chitin, pectin, lipids and proteins, while Rhodococcus sp. exhibited urease activity. Three potentially fungicolous fungi were isolated from diseased white truffle ascocarps and bioassayed against the isolated Actinobacteria. The Rhodococcus isolate inhibited Verticillium leptobactrum, neither bacterium affected Clonostachys rosea, while both isolates promoted growth of Trichoderma sp. The results suggest that Actinobacteria might be involved in improving truffle nutrition, ascocarp degradation and establishing relationships with other soil fungi.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Fungal Ecology",
title = "Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi",
pages = "538-527",
number = "6",
volume = "6",
doi = "10.1016/j.funeco.2013.05.006"
}

Pavić, A. B., Stanković, S., Saljnikov, E., Krueger, D., Buscot, F., Tarkka, M.,& Marjanović, Ž.. (2013). Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi. in Fungal Ecology
Elsevier Sci Ltd, Oxford., 6(6), 527-538.
https://doi.org/10.1016/j.funeco.2013.05.006

Pavić AB, Stanković S, Saljnikov E, Krueger D, Buscot F, Tarkka M, Marjanović Ž. Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi. in Fungal Ecology. 2013;6(6):527-538.
doi:10.1016/j.funeco.2013.05.006 .

Pavić, Aleksandar B, Stanković, Slaviša, Saljnikov, Elmira, Krueger, Dirk, Buscot, Francois, Tarkka, Mika, Marjanović, Žaklina, "Actinobacteria may influence white truffle (Tuber magnatum Pico) nutrition, ascocarp degradation and interactions with other soil fungi" in Fungal Ecology, 6, no. 6 (2013):527-538,
https://doi.org/10.1016/j.funeco.2013.05.006 . .

TY  - JOUR
AU  - Cakmak, Dragan
AU  - Saljnikov, Elmira
AU  - Mrvić, Vesna
AU  - Jakovljević, Miodrag D
AU  - Marjanović, Žaklina
AU  - Sikiric, Biljana
AU  - Maksimović, Srboljub
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/387
AB  - Long-term application of P fertilizers may eventually result: in excess and/or toxic accumulations of trace elements and microelements in soil. The effect of monoammonium-phosphate (MAP) on basic soil properties (pH, CEC, texture), the total content of C N, and F, hot acid-extractable Cu, Zn, Ni, Cr, Pb, Cd, Co, As, Hg, and F, and the content of extractable macro- and trace elements (P, K, Ca, Mg, Al, Fe, Zn, Cu, Ni, Pb, Cd, F) were Studied on a Stagnosol soil. Phosphate fertilizer had been applied (26, 39, and 52 kg P ha(-1)) over a 40-yr period. Phosphorus fertilization significantly decreased pH and increased clay content of the soil. Increases were detected in available P, exchangeable Al, Ca, cation exchange capacity (CEC), and clay content. The content of hot acid-ex tractable Pb increased, whereas the content of diethylenetriaminepentaacetic acid-extractable Pb decreased in accordance with applied rates of MAP. The status of some hot acid-extractable trace elements (Cu, Zn, Ni, and Co) did not change after 40 yr of MAP application, whereas Hg and Cd increased. However, despite the statistically significant increases in the amounts of some potentially toxic elements, they did riot accumulate to concentrations considered toxic as overall concentrations are far below the maximum allowed concentrations for natural unpolluted soils.
PB  - Wiley, Hoboken
T2  - Journal of Environmental Quality
T1  - Soil Properties and Trace Elements Contents Following 40 Years of Phosphate Fertilization
EP  - 547
IS  - 2
SP  - 541
VL  - 39
DO  - 10.2134/jeq2009.0216
ER  - 

@article{
author = "Cakmak, Dragan and Saljnikov, Elmira and Mrvić, Vesna and Jakovljević, Miodrag D and Marjanović, Žaklina and Sikiric, Biljana and Maksimović, Srboljub",
year = "2010",
abstract = "Long-term application of P fertilizers may eventually result: in excess and/or toxic accumulations of trace elements and microelements in soil. The effect of monoammonium-phosphate (MAP) on basic soil properties (pH, CEC, texture), the total content of C N, and F, hot acid-extractable Cu, Zn, Ni, Cr, Pb, Cd, Co, As, Hg, and F, and the content of extractable macro- and trace elements (P, K, Ca, Mg, Al, Fe, Zn, Cu, Ni, Pb, Cd, F) were Studied on a Stagnosol soil. Phosphate fertilizer had been applied (26, 39, and 52 kg P ha(-1)) over a 40-yr period. Phosphorus fertilization significantly decreased pH and increased clay content of the soil. Increases were detected in available P, exchangeable Al, Ca, cation exchange capacity (CEC), and clay content. The content of hot acid-ex tractable Pb increased, whereas the content of diethylenetriaminepentaacetic acid-extractable Pb decreased in accordance with applied rates of MAP. The status of some hot acid-extractable trace elements (Cu, Zn, Ni, and Co) did not change after 40 yr of MAP application, whereas Hg and Cd increased. However, despite the statistically significant increases in the amounts of some potentially toxic elements, they did riot accumulate to concentrations considered toxic as overall concentrations are far below the maximum allowed concentrations for natural unpolluted soils.",
publisher = "Wiley, Hoboken",
journal = "Journal of Environmental Quality",
title = "Soil Properties and Trace Elements Contents Following 40 Years of Phosphate Fertilization",
pages = "547-541",
number = "2",
volume = "39",
doi = "10.2134/jeq2009.0216"
}

Cakmak, D., Saljnikov, E., Mrvić, V., Jakovljević, M. D., Marjanović, Ž., Sikiric, B.,& Maksimović, S.. (2010). Soil Properties and Trace Elements Contents Following 40 Years of Phosphate Fertilization. in Journal of Environmental Quality
Wiley, Hoboken., 39(2), 541-547.
https://doi.org/10.2134/jeq2009.0216

Cakmak D, Saljnikov E, Mrvić V, Jakovljević MD, Marjanović Ž, Sikiric B, Maksimović S. Soil Properties and Trace Elements Contents Following 40 Years of Phosphate Fertilization. in Journal of Environmental Quality. 2010;39(2):541-547.
doi:10.2134/jeq2009.0216 .

Cakmak, Dragan, Saljnikov, Elmira, Mrvić, Vesna, Jakovljević, Miodrag D, Marjanović, Žaklina, Sikiric, Biljana, Maksimović, Srboljub, "Soil Properties and Trace Elements Contents Following 40 Years of Phosphate Fertilization" in Journal of Environmental Quality, 39, no. 2 (2010):541-547,
https://doi.org/10.2134/jeq2009.0216 . .

TY  - JOUR
AU  - Saljnikov, Elmira
AU  - Cakmak, Dragan
AU  - Kostić Kravljanac, Ljiljana
AU  - Maksimović, Srboljub
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/326
AB  - Characterization of Labile Fractions of Soil Organic Carbon in Mollisols from different climatic regions. The research studied the potential mineralization and characterization of labile SOM of different types of Mollisols: Hapludolls, Argiudolls, Calciustolls, and Haplustolls. Labile C fractions distributed oppositely than total SOC and were the greatest in Calciustolls and the lowest in Hapludolls. The amount of soil microbial biomass C (MBC) was significantly higher in dry than in wet regions. The highest amount of light fraction C was in dry-thermic followed by dry-frigid, and the least amount was in wet regions. Dry regions were able to retain a higher level of labile C than the moist regions, due to a slower annual decomposition rate.
PB  - Pisa Univ Press, Pisa
T2  - Agrochimica
T1  - Labile fractions of soil organic carbon in mollisols from different climatic regions
EP  - 385
IS  - 6
SP  - 376
VL  - 53
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_326
ER  - 

@article{
author = "Saljnikov, Elmira and Cakmak, Dragan and Kostić Kravljanac, Ljiljana and Maksimović, Srboljub",
year = "2009",
abstract = "Characterization of Labile Fractions of Soil Organic Carbon in Mollisols from different climatic regions. The research studied the potential mineralization and characterization of labile SOM of different types of Mollisols: Hapludolls, Argiudolls, Calciustolls, and Haplustolls. Labile C fractions distributed oppositely than total SOC and were the greatest in Calciustolls and the lowest in Hapludolls. The amount of soil microbial biomass C (MBC) was significantly higher in dry than in wet regions. The highest amount of light fraction C was in dry-thermic followed by dry-frigid, and the least amount was in wet regions. Dry regions were able to retain a higher level of labile C than the moist regions, due to a slower annual decomposition rate.",
publisher = "Pisa Univ Press, Pisa",
journal = "Agrochimica",
title = "Labile fractions of soil organic carbon in mollisols from different climatic regions",
pages = "385-376",
number = "6",
volume = "53",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_326"
}

Saljnikov, E., Cakmak, D., Kostić Kravljanac, L.,& Maksimović, S.. (2009). Labile fractions of soil organic carbon in mollisols from different climatic regions. in Agrochimica
Pisa Univ Press, Pisa., 53(6), 376-385.
https://hdl.handle.net/21.15107/rcub_rimsi_326

Saljnikov E, Cakmak D, Kostić Kravljanac L, Maksimović S. Labile fractions of soil organic carbon in mollisols from different climatic regions. in Agrochimica. 2009;53(6):376-385.
https://hdl.handle.net/21.15107/rcub_rimsi_326 .

Saljnikov, Elmira, Cakmak, Dragan, Kostić Kravljanac, Ljiljana, Maksimović, Srboljub, "Labile fractions of soil organic carbon in mollisols from different climatic regions" in Agrochimica, 53, no. 6 (2009):376-385,
https://hdl.handle.net/21.15107/rcub_rimsi_326 .