TY  - JOUR
AU  - Djalović, Ivica
AU  - Mitrović, Petar
AU  - Trivan, Goran
AU  - Jelušić, Aleksandra
AU  - Pezo, Lato
AU  - Janić Hajnal, Elizabet
AU  - Popović Milovanović, Tatjana
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3208
AB  - Infections with phytoplasma present one of the most significant biotic stresses influencing
plant health, growth, and production. The phytoplasma ‘Candidatus Phytoplasma solani’ infects
a variety of plant species. This pathogen impacts the physiological and morphological characteristics of plants causing stunting, yellowing, leaf curling, and other symptoms that can lead to significant economic losses. The aim of this study was to determine biochemical changes in peony (Paeonia tenuifolia L.), mint (Mentha × piperita L.), and dill (Anethum graveolens L.) induced by ‘Ca. Phytoplasma solani’ in Serbia as well as to predict the impact of the biotic stress using artificial neural network (ANN) modeling. The phylogenetic position of the Serbian ‘Ca. Phytoplasma solani’ strains originated from the tested hosts using 16S rRNA (peony and carrot strains) and plsC (mint and dill strains) sequences indicated by their genetic homogeneity despite the host of origin. Biochemical parameters significantly differed in asymptomatic and symptomatic plants, except for total anthocyanidins contents in dill and the capacity of peony and mint extracts to neutralize superoxide anions and hydroxyl radicals, respectively. Principal Component Analysis (PCA) showed a correlation between different chemical parameters and revealed a clear separation among the samples. Based on the ANN performance, the optimal number of hidden neurons for the calculation of TS, RG, PAL, LP, NBT, •OH, TP, TT, Tflav, Tpro, Tant, DPPH, and Car was nine (using MLP 8-9-13), as it produced high r2 values (1.000 during the training period) and low SOS values. Developing an effective early warning system for the detection of plant diseases in different plant species is critical for improving crop yield and quality.
PB  - MDPI
T2  - Horticulturae
T1  - The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach
IS  - 5
SP  - 426
VL  - 10
DO  - 10.3390/horticulturae10050426
ER  - 

@article{
author = "Djalović, Ivica and Mitrović, Petar and Trivan, Goran and Jelušić, Aleksandra and Pezo, Lato and Janić Hajnal, Elizabet and Popović Milovanović, Tatjana",
year = "2024",
abstract = "Infections with phytoplasma present one of the most significant biotic stresses influencing
plant health, growth, and production. The phytoplasma ‘Candidatus Phytoplasma solani’ infects
a variety of plant species. This pathogen impacts the physiological and morphological characteristics of plants causing stunting, yellowing, leaf curling, and other symptoms that can lead to significant economic losses. The aim of this study was to determine biochemical changes in peony (Paeonia tenuifolia L.), mint (Mentha × piperita L.), and dill (Anethum graveolens L.) induced by ‘Ca. Phytoplasma solani’ in Serbia as well as to predict the impact of the biotic stress using artificial neural network (ANN) modeling. The phylogenetic position of the Serbian ‘Ca. Phytoplasma solani’ strains originated from the tested hosts using 16S rRNA (peony and carrot strains) and plsC (mint and dill strains) sequences indicated by their genetic homogeneity despite the host of origin. Biochemical parameters significantly differed in asymptomatic and symptomatic plants, except for total anthocyanidins contents in dill and the capacity of peony and mint extracts to neutralize superoxide anions and hydroxyl radicals, respectively. Principal Component Analysis (PCA) showed a correlation between different chemical parameters and revealed a clear separation among the samples. Based on the ANN performance, the optimal number of hidden neurons for the calculation of TS, RG, PAL, LP, NBT, •OH, TP, TT, Tflav, Tpro, Tant, DPPH, and Car was nine (using MLP 8-9-13), as it produced high r2 values (1.000 during the training period) and low SOS values. Developing an effective early warning system for the detection of plant diseases in different plant species is critical for improving crop yield and quality.",
publisher = "MDPI",
journal = "Horticulturae",
title = "The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach",
number = "5",
pages = "426",
volume = "10",
doi = "10.3390/horticulturae10050426"
}

Djalović, I., Mitrović, P., Trivan, G., Jelušić, A., Pezo, L., Janić Hajnal, E.,& Popović Milovanović, T.. (2024). The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach. in Horticulturae
MDPI., 10(5), 426.
https://doi.org/10.3390/horticulturae10050426

Djalović I, Mitrović P, Trivan G, Jelušić A, Pezo L, Janić Hajnal E, Popović Milovanović T. The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach. in Horticulturae. 2024;10(5):426.
doi:10.3390/horticulturae10050426 .

Djalović, Ivica, Mitrović, Petar, Trivan, Goran, Jelušić, Aleksandra, Pezo, Lato, Janić Hajnal, Elizabet, Popović Milovanović, Tatjana, "The Effect of Biotic Stress in Plant Species Induced by ‘Candidatus Phytoplasma solani’—An Artificial Neural Network Approach" in Horticulturae, 10, no. 5 (2024):426,
https://doi.org/10.3390/horticulturae10050426 . .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović Milovanović, Tatjana
AU  - Mitrović, Petar
AU  - Stanisavljević, Rade
AU  - Janakiev, Tamara
AU  - Fira, Đorđe
AU  - Dimkić, Ivica
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2047
AB  - The potential of two biocontrol strains, Bacillus velezensis X5-2 and Pseudomonas orientalis X2-1P to control black rot caused by Xanthomonas campestris pv. campestris was evaluated in vivo on autochthonous cabbage cultivar ‘Futoški’, under the condition of natural infection. Liquid
formulations of biocontrol strains were prepared in fermenters (B. velezensis - 1010 CFU mL-1, P. orientalis - 109 CFU mL-1) and diluted with water in a 1:5 ratio, before use. Treatments were performed with each strain and their mixture (1:1). The experiment included the following: (i)
seed treatments (15, 30, 60 min), (ii) foliar treatments (three treatments: when the first symptoms appeared and remaining every two weeks), and (iii) combined seed and foliar treatments. Experiments were conducted in four replicates, with 10 plants per replicate. Two controls were
used, untreated and conventionally treated cabbage (pesticides). Assessment of disease intensity was rated one month after the last foliar treatment, using the Horsfall-Barratt scale (1-12). During ripening, cabbage heads’ weights were measured and data were statistically processed (Minitab). All performed treatments were effective in the control of black rot (85.7-98.9%). The highest efficacy was obtained in combined seed (60 min) and foliar treatments with P. orientalis, as well as its mixture with B. velezensis (98.9% both). All treatments influenced the increase of cabbage heads’ weight (up to 1.3 times), with the highest found in combined treatments (60 min seed + foliar) using a mixture of B. velezensis and P. orientalis. A negative correlation (P≤0.05) was found
between disease intensity and cabbage heads’ weight.
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria; Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy
T1  - Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’
EP  - 147
SP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2047
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović Milovanović, Tatjana and Mitrović, Petar and Stanisavljević, Rade and Janakiev, Tamara and Fira, Đorđe and Dimkić, Ivica",
year = "2022",
abstract = "The potential of two biocontrol strains, Bacillus velezensis X5-2 and Pseudomonas orientalis X2-1P to control black rot caused by Xanthomonas campestris pv. campestris was evaluated in vivo on autochthonous cabbage cultivar ‘Futoški’, under the condition of natural infection. Liquid
formulations of biocontrol strains were prepared in fermenters (B. velezensis - 1010 CFU mL-1, P. orientalis - 109 CFU mL-1) and diluted with water in a 1:5 ratio, before use. Treatments were performed with each strain and their mixture (1:1). The experiment included the following: (i)
seed treatments (15, 30, 60 min), (ii) foliar treatments (three treatments: when the first symptoms appeared and remaining every two weeks), and (iii) combined seed and foliar treatments. Experiments were conducted in four replicates, with 10 plants per replicate. Two controls were
used, untreated and conventionally treated cabbage (pesticides). Assessment of disease intensity was rated one month after the last foliar treatment, using the Horsfall-Barratt scale (1-12). During ripening, cabbage heads’ weights were measured and data were statistically processed (Minitab). All performed treatments were effective in the control of black rot (85.7-98.9%). The highest efficacy was obtained in combined seed (60 min) and foliar treatments with P. orientalis, as well as its mixture with B. velezensis (98.9% both). All treatments influenced the increase of cabbage heads’ weight (up to 1.3 times), with the highest found in combined treatments (60 min seed + foliar) using a mixture of B. velezensis and P. orientalis. A negative correlation (P≤0.05) was found
between disease intensity and cabbage heads’ weight.",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria; Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy",
title = "Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’",
pages = "147-147",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2047"
}

Jelušić, A., Popović Milovanović, T., Mitrović, P., Stanisavljević, R., Janakiev, T., Fira, Đ.,& Dimkić, I.. (2022). Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’. in 14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria; Progetto grafico - editoriale esecutivo, Diletta Pini., 147-147.
https://hdl.handle.net/21.15107/rcub_rimsi_2047

Jelušić A, Popović Milovanović T, Mitrović P, Stanisavljević R, Janakiev T, Fira Đ, Dimkić I. Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’. in 14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy. 2022;:147-147.
https://hdl.handle.net/21.15107/rcub_rimsi_2047 .

Jelušić, Aleksandra, Popović Milovanović, Tatjana, Mitrović, Petar, Stanisavljević, Rade, Janakiev, Tamara, Fira, Đorđe, Dimkić, Ivica, "Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’" in 14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy (2022):147-147,
https://hdl.handle.net/21.15107/rcub_rimsi_2047 .