TY  - JOUR
AU  - Oalde, Mariana
AU  - Kolarević, Stoimir
AU  - Živković, Jelena
AU  - Alimpic-Aradski, Ana
AU  - Jovanović-Marić, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Marin, Petar D.
AU  - Savikin, Katarina
AU  - Vukovic-Gacic, Branka
AU  - Duletic-Lausević, Sonja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1454
AB  - This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g(-1)), naringin (up to 5.07 mg g(-1)) and luteolin-7-O-glucoside (up to 3.54 mg g(-1)). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro
EP  - 3245
IS  - 7
SP  - 3233
VL  - 12
DO  - 10.1039/d1fo00447f
ER  - 

@article{
author = "Oalde, Mariana and Kolarević, Stoimir and Živković, Jelena and Alimpic-Aradski, Ana and Jovanović-Marić, Jovana and Kracun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Marin, Petar D. and Savikin, Katarina and Vukovic-Gacic, Branka and Duletic-Lausević, Sonja",
year = "2021",
abstract = "This research was aimed to assess the potential of Glechoma hederacea, Hyssopus officinalis, Lavandula angustifolia, Leonurus cardiaca, Marrubium vulgare and Sideritis scardica (Lamiaceae) methanolic, ethanolic and aqueous extracts against the damaging effects of oxidative stress using different experimental models. The chemical characterization was done spectrophotometrically by quantifying total phenolics, phenolic acids, flavonoids and flavonols in the extracts, as well as by employing HPLC-DAD technique. Moreover, DPPH assay was used to assess the extracts' radical scavenging potential. Genoprotective properties of the extracts were evaluated using plasmid pUC19 Escherichia coli XL1-Blue, whereas their antigenotoxic potential was determined using Salmonella typhimurium TA1535/pSK1002 and normal human lung fibroblasts. All of the extracts showed antioxidant activity in DPPH assay. Furthermore, the results have shown that aqueous extracts provided the best protection for plasmid DNA, while alcoholic extracts most effectively contributed to the preservation of prokaryotic DNA. Additionally, each of the tested samples significantly protected the eukaryotic cells against genomic damages. Finally, despite not showing exceptional results in DPPH assay, S. scardica extracts are regarded as the most favorable in maintaining the integrity of DNA, which might be due to high quantities of phenolics such as quercetin (up to 17.95 mg g(-1)), naringin (up to 5.07 mg g(-1)) and luteolin-7-O-glucoside (up to 3.54 mg g(-1)). Overall, this comprehensive concept highlights the ability of these Lamiaceae species to safeguard the DNA from reactive oxygen species, to curtail the inflicted damage and also improve the efficiency of the DNA repair mechanisms, while emphasizing the importance of polyphenols as their active principles.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro",
pages = "3245-3233",
number = "7",
volume = "12",
doi = "10.1039/d1fo00447f"
}

Oalde, M., Kolarević, S., Živković, J., Alimpic-Aradski, A., Jovanović-Marić, J., Kracun-Kolarević, M., Đorđević Aleksić, J., Marin, P. D., Savikin, K., Vukovic-Gacic, B.,& Duletic-Lausević, S.. (2021). A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function
Royal Soc Chemistry, Cambridge., 12(7), 3233-3245.
https://doi.org/10.1039/d1fo00447f

Oalde M, Kolarević S, Živković J, Alimpic-Aradski A, Jovanović-Marić J, Kracun-Kolarević M, Đorđević Aleksić J, Marin PD, Savikin K, Vukovic-Gacic B, Duletic-Lausević S. A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro. in Food & Function. 2021;12(7):3233-3245.
doi:10.1039/d1fo00447f .

Oalde, Mariana, Kolarević, Stoimir, Živković, Jelena, Alimpic-Aradski, Ana, Jovanović-Marić, Jovana, Kracun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Marin, Petar D., Savikin, Katarina, Vukovic-Gacic, Branka, Duletic-Lausević, Sonja, "A comprehensive assessment of the chemical composition, antioxidant, genoprotective and antigenotoxic activities of Lamiaceae species using different experimental models in vitro" in Food & Function, 12, no. 7 (2021):3233-3245,
https://doi.org/10.1039/d1fo00447f . .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Višnjić Jeftić, Željka
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1501
AB  - This study was conducted on the Danube River locality Visnjica, exposed to the discharge of the largest wastewater collector in Serbia's capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia
IS  - 8
VL  - 193
DO  - 10.1007/s10661-021-09232-6
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Višnjić Jeftić, Željka and Rasković, Božidar and Poleksic, Vesna and Gačić, Zoran and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2021",
abstract = "This study was conducted on the Danube River locality Visnjica, exposed to the discharge of the largest wastewater collector in Serbia's capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia",
number = "8",
volume = "193",
doi = "10.1007/s10661-021-09232-6"
}

Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Višnjić Jeftić, Ž., Rasković, B., Poleksic, V., Gačić, Z., Lenhardt, M.,& Vukovic-Gacic, B.. (2021). Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment
Springer, Dordrecht., 193(8).
https://doi.org/10.1007/s10661-021-09232-6

Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Višnjić Jeftić Ž, Rasković B, Poleksic V, Gačić Z, Lenhardt M, Vukovic-Gacic B. Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment. 2021;193(8).
doi:10.1007/s10661-021-09232-6 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Višnjić Jeftić, Željka, Rasković, Božidar, Poleksic, Vesna, Gačić, Zoran, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia" in Environmental Monitoring and Assessment, 193, no. 8 (2021),
https://doi.org/10.1007/s10661-021-09232-6 . .

TY  - JOUR
AU  - Pavlović, Mariana Oalde
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Jovanović-Marić, Jovana
AU  - Lunic, Tanja
AU  - Mandic, Marija
AU  - Kracun-Kolarević, Margareta
AU  - Živković, Jelena
AU  - Alimpic-Aradski, Ana
AU  - Marin, Petar D.
AU  - Savikin, Katarina
AU  - Vukovic-Gacic, Branka
AU  - Bozić-Nedeljković, Biljana
AU  - Duletic-Lausević, Sonja
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1502
AB  - This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha x piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species
IS  - 11
VL  - 10
DO  - 10.3390/plants10112306
ER  - 

@article{
author = "Pavlović, Mariana Oalde and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Jovanović-Marić, Jovana and Lunic, Tanja and Mandic, Marija and Kracun-Kolarević, Margareta and Živković, Jelena and Alimpic-Aradski, Ana and Marin, Petar D. and Savikin, Katarina and Vukovic-Gacic, Branka and Bozić-Nedeljković, Biljana and Duletic-Lausević, Sonja",
year = "2021",
abstract = "This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha x piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species",
number = "11",
volume = "10",
doi = "10.3390/plants10112306"
}

Pavlović, M. O., Kolarević, S., Đorđević Aleksić, J., Jovanović-Marić, J., Lunic, T., Mandic, M., Kracun-Kolarević, M., Živković, J., Alimpic-Aradski, A., Marin, P. D., Savikin, K., Vukovic-Gacic, B., Bozić-Nedeljković, B.,& Duletic-Lausević, S.. (2021). A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants-Basel
MDPI, Basel., 10(11).
https://doi.org/10.3390/plants10112306

Pavlović MO, Kolarević S, Đorđević Aleksić J, Jovanović-Marić J, Lunic T, Mandic M, Kracun-Kolarević M, Živković J, Alimpic-Aradski A, Marin PD, Savikin K, Vukovic-Gacic B, Bozić-Nedeljković B, Duletic-Lausević S. A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants-Basel. 2021;10(11).
doi:10.3390/plants10112306 .

Pavlović, Mariana Oalde, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Jovanović-Marić, Jovana, Lunic, Tanja, Mandic, Marija, Kracun-Kolarević, Margareta, Živković, Jelena, Alimpic-Aradski, Ana, Marin, Petar D., Savikin, Katarina, Vukovic-Gacic, Branka, Bozić-Nedeljković, Biljana, Duletic-Lausević, Sonja, "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species" in Plants-Basel, 10, no. 11 (2021),
https://doi.org/10.3390/plants10112306 . .

TY  - JOUR
AU  - Radovanović, Jelena
AU  - Antonijević, Biljana
AU  - Kolarević, Stoimir
AU  - Milutinovic-Smiljanic, Sanja
AU  - Mandic, Jelena
AU  - Vukovic-Gacic, Branka
AU  - Bulat, Zorica
AU  - Curcic, Marijana
AU  - Kracun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Jovanović-Marić, Jovana
AU  - Antonijevic-Miljaković, Evica
AU  - Dukic-Cosic, Danijela
AU  - Đorđević, Aleksandra Buha
AU  - Javorac, Dragana
AU  - Baralic, Katarina
AU  - Mandinic, Zoran
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1505
AB  - The aims of this study were to: (i) examine the toxic effects of sodium fluoride (NaF) in blood, liver, spleen, and brain cells of Wistar rats after the subacute exposure; (ii) explore the potential protective properties of selenium (Se) against fluoride toxicity after the simultaneous administration. Twenty male Wistar rats, eight weeks old, weighing approximately 140-190 g, were divided into four experimental groups (n = 5) as follows: I control-tap water; II NaF 150 ppm; III NaF 150 ppm and Se 1.5 mg/L; IV Se 1.5 mg/L, and had available water with solutions ad libitum for 28 days. DNA damage detected by comet assay was confirmed in the liver, spleen, and brain cells, but not in blood. Selenium supplementation together with NaF decreased DNA damage in liver and spleen cells. According to the histological findings, no changes were observed in spleen and brain tissues after NaF administration. Unlike the observed Se protective effect on the DNA level, no significant reduction of liver tissue injury was observed after the NaF and Se treatment, resulting in mild inflammation. Data of this study suggest that DNA damage after NaF subacute exposure at moderately high concentration was reduced in liver and spleen cells due to Se supplementation, but a similar change was not seen in the brain.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Chemosphere
T1  - Genotoxicity of fluoride subacute exposure in rats and selenium intervention
VL  - 266
DO  - 10.1016/j.chemosphere.2020.128978
ER  - 

@article{
author = "Radovanović, Jelena and Antonijević, Biljana and Kolarević, Stoimir and Milutinovic-Smiljanic, Sanja and Mandic, Jelena and Vukovic-Gacic, Branka and Bulat, Zorica and Curcic, Marijana and Kracun-Kolarević, Margareta and Sunjog, Karolina and Kostić-Vuković, Jovana and Jovanović-Marić, Jovana and Antonijevic-Miljaković, Evica and Dukic-Cosic, Danijela and Đorđević, Aleksandra Buha and Javorac, Dragana and Baralic, Katarina and Mandinic, Zoran",
year = "2021",
abstract = "The aims of this study were to: (i) examine the toxic effects of sodium fluoride (NaF) in blood, liver, spleen, and brain cells of Wistar rats after the subacute exposure; (ii) explore the potential protective properties of selenium (Se) against fluoride toxicity after the simultaneous administration. Twenty male Wistar rats, eight weeks old, weighing approximately 140-190 g, were divided into four experimental groups (n = 5) as follows: I control-tap water; II NaF 150 ppm; III NaF 150 ppm and Se 1.5 mg/L; IV Se 1.5 mg/L, and had available water with solutions ad libitum for 28 days. DNA damage detected by comet assay was confirmed in the liver, spleen, and brain cells, but not in blood. Selenium supplementation together with NaF decreased DNA damage in liver and spleen cells. According to the histological findings, no changes were observed in spleen and brain tissues after NaF administration. Unlike the observed Se protective effect on the DNA level, no significant reduction of liver tissue injury was observed after the NaF and Se treatment, resulting in mild inflammation. Data of this study suggest that DNA damage after NaF subacute exposure at moderately high concentration was reduced in liver and spleen cells due to Se supplementation, but a similar change was not seen in the brain.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Chemosphere",
title = "Genotoxicity of fluoride subacute exposure in rats and selenium intervention",
volume = "266",
doi = "10.1016/j.chemosphere.2020.128978"
}

Radovanović, J., Antonijević, B., Kolarević, S., Milutinovic-Smiljanic, S., Mandic, J., Vukovic-Gacic, B., Bulat, Z., Curcic, M., Kracun-Kolarević, M., Sunjog, K., Kostić-Vuković, J., Jovanović-Marić, J., Antonijevic-Miljaković, E., Dukic-Cosic, D., Đorđević, A. B., Javorac, D., Baralic, K.,& Mandinic, Z.. (2021). Genotoxicity of fluoride subacute exposure in rats and selenium intervention. in Chemosphere
Pergamon-Elsevier Science Ltd, Oxford., 266.
https://doi.org/10.1016/j.chemosphere.2020.128978

Radovanović J, Antonijević B, Kolarević S, Milutinovic-Smiljanic S, Mandic J, Vukovic-Gacic B, Bulat Z, Curcic M, Kracun-Kolarević M, Sunjog K, Kostić-Vuković J, Jovanović-Marić J, Antonijevic-Miljaković E, Dukic-Cosic D, Đorđević AB, Javorac D, Baralic K, Mandinic Z. Genotoxicity of fluoride subacute exposure in rats and selenium intervention. in Chemosphere. 2021;266.
doi:10.1016/j.chemosphere.2020.128978 .

Radovanović, Jelena, Antonijević, Biljana, Kolarević, Stoimir, Milutinovic-Smiljanic, Sanja, Mandic, Jelena, Vukovic-Gacic, Branka, Bulat, Zorica, Curcic, Marijana, Kracun-Kolarević, Margareta, Sunjog, Karolina, Kostić-Vuković, Jovana, Jovanović-Marić, Jovana, Antonijevic-Miljaković, Evica, Dukic-Cosic, Danijela, Đorđević, Aleksandra Buha, Javorac, Dragana, Baralic, Katarina, Mandinic, Zoran, "Genotoxicity of fluoride subacute exposure in rats and selenium intervention" in Chemosphere, 266 (2021),
https://doi.org/10.1016/j.chemosphere.2020.128978 . .

TY  - JOUR
AU  - Jovanović-Maric, Jovana M.
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Đorđević Aleksić, Jelena
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Smiljanic, Petar B.
AU  - Gačić, Zoran
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1369
AB  - This study deals with bleak (Alburnus alburnus) sensitivity in detecting of the wastewater related pressure in large lowland rivers. The major objective was to investigate if the response measured in bleak should be linked to a certain stretch of the river and characterised as "stretch specific", or it should be linked to the sampling site and characterised as "site specific". The response was evaluated via condition index, metal pollution index, DNA damage and cell viability using integrated biomarker response approach. The study was conducted at 3 sub-sites characterized by different pollution levels in a relatively short stretch (2 km) of the Sava River (Serbia). Results indicated that the response of the biomarkers in bleak can be interpreted as "site specific". Among the studied biomarkers, DNA damage assessed by comet assay and micronucleus test has shown high sensitivity in differentiation of the sites.
PB  - Springer, New York
T2  - Bulletin of Environmental Contamination and Toxicology
T1  - Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers
EP  - 229
IS  - 2
SP  - 224
VL  - 105
DO  - 10.1007/s00128-020-02944-4
ER  - 

@article{
author = "Jovanović-Maric, Jovana M. and Kracun-Kolarević, Margareta and Kolarević, Stoimir and Đorđević Aleksić, Jelena and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Smiljanic, Petar B. and Gačić, Zoran and Vukovic-Gacic, Branka",
year = "2020",
abstract = "This study deals with bleak (Alburnus alburnus) sensitivity in detecting of the wastewater related pressure in large lowland rivers. The major objective was to investigate if the response measured in bleak should be linked to a certain stretch of the river and characterised as "stretch specific", or it should be linked to the sampling site and characterised as "site specific". The response was evaluated via condition index, metal pollution index, DNA damage and cell viability using integrated biomarker response approach. The study was conducted at 3 sub-sites characterized by different pollution levels in a relatively short stretch (2 km) of the Sava River (Serbia). Results indicated that the response of the biomarkers in bleak can be interpreted as "site specific". Among the studied biomarkers, DNA damage assessed by comet assay and micronucleus test has shown high sensitivity in differentiation of the sites.",
publisher = "Springer, New York",
journal = "Bulletin of Environmental Contamination and Toxicology",
title = "Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers",
pages = "229-224",
number = "2",
volume = "105",
doi = "10.1007/s00128-020-02944-4"
}

Jovanović-Maric, J. M., Kracun-Kolarević, M., Kolarević, S., Đorđević Aleksić, J., Paunović, M., Kostić-Vuković, J., Sunjog, K., Smiljanic, P. B., Gačić, Z.,& Vukovic-Gacic, B.. (2020). Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers. in Bulletin of Environmental Contamination and Toxicology
Springer, New York., 105(2), 224-229.
https://doi.org/10.1007/s00128-020-02944-4

Jovanović-Maric JM, Kracun-Kolarević M, Kolarević S, Đorđević Aleksić J, Paunović M, Kostić-Vuković J, Sunjog K, Smiljanic PB, Gačić Z, Vukovic-Gacic B. Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers. in Bulletin of Environmental Contamination and Toxicology. 2020;105(2):224-229.
doi:10.1007/s00128-020-02944-4 .

Jovanović-Maric, Jovana M., Kracun-Kolarević, Margareta, Kolarević, Stoimir, Đorđević Aleksić, Jelena, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Smiljanic, Petar B., Gačić, Zoran, Vukovic-Gacic, Branka, "Sensitivity of Bleak (Alburnus alburnus) in Detection of the Wastewater Related Pressure in Large Lowland Rivers" in Bulletin of Environmental Contamination and Toxicology, 105, no. 2 (2020):224-229,
https://doi.org/10.1007/s00128-020-02944-4 . .

TY  - JOUR
AU  - Đorđević Aleksić, Jelena
AU  - Kolarević, Stoimir
AU  - Jovanović, Jovana
AU  - Kostić-Vuković, Jovana
AU  - Novaković, Irena
AU  - Jeremic, Marko
AU  - Sladic, Dusan
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1365
AB  - Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuCassay inSalmonella typhimuriumTA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2). Results indicated that in the acellular model TBQ and its derivatives do not interact with plasmid pUC19. In the prokaryotic model, only TBQ exerted weak genotoxic potential and only at highly cytotoxic concentrations. In eukaryotic models, genotoxic potential was detected mainly at the highest concentrations of the tested substances but the effect was lower in both cell lines in comparison with benzo[a]pyrene and etoposide which were used as positive controls. Weak genotoxic potential of tested compounds recommends them as good candidates for further testing in development of new antitumor agents.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Drug and Chemical Toxicology
T1  - Evaluation of genotoxic potential oftert-butylquinone and its derivatives in prokaryotic and eukaryotic test models
EP  - 530
IS  - 5
SP  - 522
VL  - 43
DO  - 10.1080/01480545.2018.1514043
ER  - 

@article{
author = "Đorđević Aleksić, Jelena and Kolarević, Stoimir and Jovanović, Jovana and Kostić-Vuković, Jovana and Novaković, Irena and Jeremic, Marko and Sladic, Dusan and Vukovic-Gacic, Branka",
year = "2020",
abstract = "Tert-butylquinone (TBQ) and its alkylamino and aralkylamino derivatives are of high interest as a potential antitumor agent. Therefore, it was necessary to investigate if the compounds exert undesirable activities such as interaction with DNA molecule which could result in negative side effects in the case of their use in the diseases treatment. The major aim of this study was to investigate genotoxic potential of TBQ and selected derivatives in an acellular model by using plasmid DNA, in the prokaryotic model by the SOS/umuCassay inSalmonella typhimuriumTA1535/pSK1002 and in eukaryotic models by using comet assay in human fetal lung cell line (MRC-5) and human liver cancer cell line (HepG2). Results indicated that in the acellular model TBQ and its derivatives do not interact with plasmid pUC19. In the prokaryotic model, only TBQ exerted weak genotoxic potential and only at highly cytotoxic concentrations. In eukaryotic models, genotoxic potential was detected mainly at the highest concentrations of the tested substances but the effect was lower in both cell lines in comparison with benzo[a]pyrene and etoposide which were used as positive controls. Weak genotoxic potential of tested compounds recommends them as good candidates for further testing in development of new antitumor agents.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Drug and Chemical Toxicology",
title = "Evaluation of genotoxic potential oftert-butylquinone and its derivatives in prokaryotic and eukaryotic test models",
pages = "530-522",
number = "5",
volume = "43",
doi = "10.1080/01480545.2018.1514043"
}

Đorđević Aleksić, J., Kolarević, S., Jovanović, J., Kostić-Vuković, J., Novaković, I., Jeremic, M., Sladic, D.,& Vukovic-Gacic, B.. (2020). Evaluation of genotoxic potential oftert-butylquinone and its derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology
Taylor & Francis Ltd, Abingdon., 43(5), 522-530.
https://doi.org/10.1080/01480545.2018.1514043

Đorđević Aleksić J, Kolarević S, Jovanović J, Kostić-Vuković J, Novaković I, Jeremic M, Sladic D, Vukovic-Gacic B. Evaluation of genotoxic potential oftert-butylquinone and its derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology. 2020;43(5):522-530.
doi:10.1080/01480545.2018.1514043 .

Đorđević Aleksić, Jelena, Kolarević, Stoimir, Jovanović, Jovana, Kostić-Vuković, Jovana, Novaković, Irena, Jeremic, Marko, Sladic, Dusan, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential oftert-butylquinone and its derivatives in prokaryotic and eukaryotic test models" in Drug and Chemical Toxicology, 43, no. 5 (2020):522-530,
https://doi.org/10.1080/01480545.2018.1514043 . .

TY  - JOUR
AU  - Oalde, Mariana
AU  - Kolarević, Stoimir
AU  - Živković, Jelena C.
AU  - Vukovic-Gacic, Branka
AU  - Jovanović-Marić, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Đorđević Aleksić, Jelena
AU  - Alimpic-Aradski, Ana
AU  - Marin, Petar D.
AU  - Savikin, Katarina
AU  - Duletic-Lausević, Sonja N.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1326
AB  - The main objective of this research was to evaluate the impact of methanolic, ethanolic and aqueous extracts of Origanum majorana L., Origanum vulgare L., Teucrium chamaedrys L., Teucrium montanum L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) on the effects of free radicals using different model systems. The extracts were characterized on the basis of the contents of total phenolics, phenolic acids, flavonoids and flavonols, and also using high-performance liquid chromatography with diode-array detection. Antioxidant activity in vitro was assessed using DPPH assay. The genoprotective properties were tested using plasmid relaxation assay on pUC19 E. coli XL1-Blue, while SOS/umuC assay on Salmonella typhimurium TA1535/pSK1002 and Comet assay on human lung fibroblasts were used to assess the antigenotoxicity of the extracts. Ethanolic extracts had the most phenolics (up to 236.20 mg GAE/g at 0.5 mg/mL), flavonoids (up to 42.47 mg QE/g at 0.5 mg/mL) and flavonols (up to 16.56 mg QE/g at 0.5 mg/mL), and they exhibited the highest DPPH activity (up to 92.16% at 0.25 mg/mL). Interestingly enough, aqueous extracts provided the best protection of plasmid DNA (the lowest IC50 value was 0.17 mg/mL). Methanolic extracts, on the other hand, most efficiently protected the prokaryotic DNA, while all the extracts had a significant impact against genomic damages inflicted on human fibroblasts. O. vulgare extracts are considered to be the most promising in preserving the overall DNA integrity against oxidative genomic damages. Moreover, HPLC-DAD analysis highlighted rosmarinic acid as the most abundant in the investigated samples (551.45 mg/mL in total in all the extracts), followed by luteolin-7-O-glucoside (150.19 mg/mL in total), while their presence correlates with most of the displayed activities. The novelty of this study is reflected in the application of a prokaryotic model for testing the antigenotoxic effects of Lamiaceae species, as no previous reports have yet been published on the genoprotective potential of these species.
PB  - Elsevier, Amsterdam
T2  - Saudi Pharmaceutical Journal
T1  - The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro
EP  - 1604
IS  - 12
SP  - 1592
VL  - 28
DO  - 10.1016/j.jsps.2020.10.006
ER  - 

@article{
author = "Oalde, Mariana and Kolarević, Stoimir and Živković, Jelena C. and Vukovic-Gacic, Branka and Jovanović-Marić, Jovana and Kracun-Kolarević, Margareta and Đorđević Aleksić, Jelena and Alimpic-Aradski, Ana and Marin, Petar D. and Savikin, Katarina and Duletic-Lausević, Sonja N.",
year = "2020",
abstract = "The main objective of this research was to evaluate the impact of methanolic, ethanolic and aqueous extracts of Origanum majorana L., Origanum vulgare L., Teucrium chamaedrys L., Teucrium montanum L., Thymus serpyllum L. and Thymus vulgaris L. (Lamiaceae) on the effects of free radicals using different model systems. The extracts were characterized on the basis of the contents of total phenolics, phenolic acids, flavonoids and flavonols, and also using high-performance liquid chromatography with diode-array detection. Antioxidant activity in vitro was assessed using DPPH assay. The genoprotective properties were tested using plasmid relaxation assay on pUC19 E. coli XL1-Blue, while SOS/umuC assay on Salmonella typhimurium TA1535/pSK1002 and Comet assay on human lung fibroblasts were used to assess the antigenotoxicity of the extracts. Ethanolic extracts had the most phenolics (up to 236.20 mg GAE/g at 0.5 mg/mL), flavonoids (up to 42.47 mg QE/g at 0.5 mg/mL) and flavonols (up to 16.56 mg QE/g at 0.5 mg/mL), and they exhibited the highest DPPH activity (up to 92.16% at 0.25 mg/mL). Interestingly enough, aqueous extracts provided the best protection of plasmid DNA (the lowest IC50 value was 0.17 mg/mL). Methanolic extracts, on the other hand, most efficiently protected the prokaryotic DNA, while all the extracts had a significant impact against genomic damages inflicted on human fibroblasts. O. vulgare extracts are considered to be the most promising in preserving the overall DNA integrity against oxidative genomic damages. Moreover, HPLC-DAD analysis highlighted rosmarinic acid as the most abundant in the investigated samples (551.45 mg/mL in total in all the extracts), followed by luteolin-7-O-glucoside (150.19 mg/mL in total), while their presence correlates with most of the displayed activities. The novelty of this study is reflected in the application of a prokaryotic model for testing the antigenotoxic effects of Lamiaceae species, as no previous reports have yet been published on the genoprotective potential of these species.",
publisher = "Elsevier, Amsterdam",
journal = "Saudi Pharmaceutical Journal",
title = "The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro",
pages = "1604-1592",
number = "12",
volume = "28",
doi = "10.1016/j.jsps.2020.10.006"
}

Oalde, M., Kolarević, S., Živković, J. C., Vukovic-Gacic, B., Jovanović-Marić, J., Kracun-Kolarević, M., Đorđević Aleksić, J., Alimpic-Aradski, A., Marin, P. D., Savikin, K.,& Duletic-Lausević, S. N.. (2020). The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro. in Saudi Pharmaceutical Journal
Elsevier, Amsterdam., 28(12), 1592-1604.
https://doi.org/10.1016/j.jsps.2020.10.006

Oalde M, Kolarević S, Živković JC, Vukovic-Gacic B, Jovanović-Marić J, Kracun-Kolarević M, Đorđević Aleksić J, Alimpic-Aradski A, Marin PD, Savikin K, Duletic-Lausević SN. The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro. in Saudi Pharmaceutical Journal. 2020;28(12):1592-1604.
doi:10.1016/j.jsps.2020.10.006 .

Oalde, Mariana, Kolarević, Stoimir, Živković, Jelena C., Vukovic-Gacic, Branka, Jovanović-Marić, Jovana, Kracun-Kolarević, Margareta, Đorđević Aleksić, Jelena, Alimpic-Aradski, Ana, Marin, Petar D., Savikin, Katarina, Duletic-Lausević, Sonja N., "The impact of different extracts of six Lamiaceae species on deleterious effects of oxidative stress assessed in acellular, prokaryotic and eukaryotic models in vitro" in Saudi Pharmaceutical Journal, 28, no. 12 (2020):1592-1604,
https://doi.org/10.1016/j.jsps.2020.10.006 . .

TY  - CHAP
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Jovanović, J.
AU  - Đorđević Aleksić, Jelena
AU  - Ilić, M.
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Divac, Rankov, A.
AU  - Ilić, B.
AU  - Pešić, V.
AU  - Vukovic-Gacic, Branka
AU  - Paunović, Momir
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1302
AB  - Montenegrin surface water and groundwater are important for the Balkan Peninsula since they are connected by the transboundary Dinaric Karst Aquifer System with the waters of additional five countries. The pollution from the surface water can rapidly infiltrate in aquifer and endanger this sensible ecosystem and the health of humans through drinking water supply. This chapter gives insights in the pressures of pollution on Montenegrin waters and in a limited literature data regarding freshwater ecotoxicological studies in Montenegro. Also, this chapter provides new ecotoxicological data obtained during survey in 2019, with a focus on the sites which are identified as hotspots of fecal pollution. The highest responses of biomarkers which indicate embryotoxic, genotoxic, and phytotoxic effects in zebrafish embryo test and in roots of Allium cepa were obtained at Ćehotina – downstream of Pljevlja. Similar results were detected at the site downstream Mojkovac at Tara, yet this site is affected by different type of pollution. Genotoxic endpoints in zebrafish stressed out sites on Morača and Lim rivers which are under pressures of fecal pollution. The data in this chapter provides an insight into current status obtained by the ex situ bioassays and indicates need for more comprehensive in situ assessment.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Handbook of Environmental Chemistry
T1  - Impact of pollution on rivers in Montenegro: Ecotoxicological perspective
EP  - 133
SP  - 111
VL  - 93
DO  - 10.1007/698_2019_425
ER  - 

@inbook{
author = "Kracun-Kolarević, Margareta and Kolarević, Stoimir and Jovanović, J. and Đorđević Aleksić, Jelena and Ilić, M. and Sunjog, Karolina and Kostić-Vuković, Jovana and Divac, Rankov, A. and Ilić, B. and Pešić, V. and Vukovic-Gacic, Branka and Paunović, Momir",
year = "2020",
abstract = "Montenegrin surface water and groundwater are important for the Balkan Peninsula since they are connected by the transboundary Dinaric Karst Aquifer System with the waters of additional five countries. The pollution from the surface water can rapidly infiltrate in aquifer and endanger this sensible ecosystem and the health of humans through drinking water supply. This chapter gives insights in the pressures of pollution on Montenegrin waters and in a limited literature data regarding freshwater ecotoxicological studies in Montenegro. Also, this chapter provides new ecotoxicological data obtained during survey in 2019, with a focus on the sites which are identified as hotspots of fecal pollution. The highest responses of biomarkers which indicate embryotoxic, genotoxic, and phytotoxic effects in zebrafish embryo test and in roots of Allium cepa were obtained at Ćehotina – downstream of Pljevlja. Similar results were detected at the site downstream Mojkovac at Tara, yet this site is affected by different type of pollution. Genotoxic endpoints in zebrafish stressed out sites on Morača and Lim rivers which are under pressures of fecal pollution. The data in this chapter provides an insight into current status obtained by the ex situ bioassays and indicates need for more comprehensive in situ assessment.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Handbook of Environmental Chemistry",
booktitle = "Impact of pollution on rivers in Montenegro: Ecotoxicological perspective",
pages = "133-111",
volume = "93",
doi = "10.1007/698_2019_425"
}

Kracun-Kolarević, M., Kolarević, S., Jovanović, J., Đorđević Aleksić, J., Ilić, M., Sunjog, K., Kostić-Vuković, J., Divac, R. A., Ilić, B., Pešić, V., Vukovic-Gacic, B.,& Paunović, M.. (2020). Impact of pollution on rivers in Montenegro: Ecotoxicological perspective. in Handbook of Environmental Chemistry
Springer Science and Business Media Deutschland GmbH., 93, 111-133.
https://doi.org/10.1007/698_2019_425

Kracun-Kolarević M, Kolarević S, Jovanović J, Đorđević Aleksić J, Ilić M, Sunjog K, Kostić-Vuković J, Divac RA, Ilić B, Pešić V, Vukovic-Gacic B, Paunović M. Impact of pollution on rivers in Montenegro: Ecotoxicological perspective. in Handbook of Environmental Chemistry. 2020;93:111-133.
doi:10.1007/698_2019_425 .

Kracun-Kolarević, Margareta, Kolarević, Stoimir, Jovanović, J., Đorđević Aleksić, Jelena, Ilić, M., Sunjog, Karolina, Kostić-Vuković, Jovana, Divac, Rankov, A., Ilić, B., Pešić, V., Vukovic-Gacic, Branka, Paunović, Momir, "Impact of pollution on rivers in Montenegro: Ecotoxicological perspective" in Handbook of Environmental Chemistry, 93 (2020):111-133,
https://doi.org/10.1007/698_2019_425 . .

TY  - JOUR
AU  - Jovanović-Marić, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Deutschmann, Bjorn
AU  - Hollert, Henner
AU  - Tenji, Dina
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1362
AB  - In this study, few different evaluation concepts were used for the assessment of genotoxic potential at the stretch of the Danube River identified as a significant hotspot of pollution originated through the untreated wastewaters. Three sites were chosen: one site upstream of the wastewater outlet in Novi Sad (Serbia), one at the outlet of wastewaters, and one site few kilometer downstream. Ex situ approach comprised prokaryotic SOS/umuCtest onSalmonella typhimuriumTA1535/pSK1005 and comet assay on human hepatoma cell line (HepG2). In situ approach was based on the active monitoring (cage approach) using freshwater musselsSinanodonta woodianaand fishCyprinus carpio.The comet and micronucleus assays were selected for evaluation of DNA damage in mussel haemocytes and fish blood cells. Within the ex situ part of the study, our results indicated that the eukaryotic model system is more sensitive compared to the prokaryotic one. In situ bioassays are recommended for obtaining a better insight into ecosystem status and in the case of our study the complete insight of genotoxic pressure. However, the choice of animals as bioindicators also has a significant impact on the quality of the obtained information. Differential response between fish and mussels was observed at the highly polluted site suggesting possible involvement of additional protective mechanism such as valve closure in mussels.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments
EP  - 33915
IS  - 27
SP  - 33903
VL  - 27
DO  - 10.1007/s11356-020-09597-0
ER  - 

@article{
author = "Jovanović-Marić, Jovana and Kracun-Kolarević, Margareta and Kolarević, Stoimir and Sunjog, Karolina and Kostić-Vuković, Jovana and Deutschmann, Bjorn and Hollert, Henner and Tenji, Dina and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2020",
abstract = "In this study, few different evaluation concepts were used for the assessment of genotoxic potential at the stretch of the Danube River identified as a significant hotspot of pollution originated through the untreated wastewaters. Three sites were chosen: one site upstream of the wastewater outlet in Novi Sad (Serbia), one at the outlet of wastewaters, and one site few kilometer downstream. Ex situ approach comprised prokaryotic SOS/umuCtest onSalmonella typhimuriumTA1535/pSK1005 and comet assay on human hepatoma cell line (HepG2). In situ approach was based on the active monitoring (cage approach) using freshwater musselsSinanodonta woodianaand fishCyprinus carpio.The comet and micronucleus assays were selected for evaluation of DNA damage in mussel haemocytes and fish blood cells. Within the ex situ part of the study, our results indicated that the eukaryotic model system is more sensitive compared to the prokaryotic one. In situ bioassays are recommended for obtaining a better insight into ecosystem status and in the case of our study the complete insight of genotoxic pressure. However, the choice of animals as bioindicators also has a significant impact on the quality of the obtained information. Differential response between fish and mussels was observed at the highly polluted site suggesting possible involvement of additional protective mechanism such as valve closure in mussels.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments",
pages = "33915-33903",
number = "27",
volume = "27",
doi = "10.1007/s11356-020-09597-0"
}

Jovanović-Marić, J., Kracun-Kolarević, M., Kolarević, S., Sunjog, K., Kostić-Vuković, J., Deutschmann, B., Hollert, H., Tenji, D., Paunović, M.,& Vukovic-Gacic, B.. (2020). Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(27), 33903-33915.
https://doi.org/10.1007/s11356-020-09597-0

Jovanović-Marić J, Kracun-Kolarević M, Kolarević S, Sunjog K, Kostić-Vuković J, Deutschmann B, Hollert H, Tenji D, Paunović M, Vukovic-Gacic B. Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments. in Environmental Science and Pollution Research. 2020;27(27):33903-33915.
doi:10.1007/s11356-020-09597-0 .

Jovanović-Marić, Jovana, Kracun-Kolarević, Margareta, Kolarević, Stoimir, Sunjog, Karolina, Kostić-Vuković, Jovana, Deutschmann, Bjorn, Hollert, Henner, Tenji, Dina, Paunović, Momir, Vukovic-Gacic, Branka, "Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments" in Environmental Science and Pollution Research, 27, no. 27 (2020):33903-33915,
https://doi.org/10.1007/s11356-020-09597-0 . .

TY  - CHAP
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Jovanović, J.
AU  - Ilić, M.
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Martinović, Rajko
AU  - Jokanović, S.
AU  - Joksimović, D.
AU  - Pešić, V.
AU  - Kirschner, A.K.T.
AU  - Linke, R.
AU  - Ixenmaier, S.
AU  - Farnleitner, Andreas
AU  - Savio, D.
AU  - Reischer, Georg
AU  - Tomić, N.
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1310
AB  - The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Handbook of Environmental Chemistry
T1  - Microbiological water quality of rivers in Montenegro
EP  - 155
SP  - 135
VL  - 93
DO  - 10.1007/698_2019_420
ER  - 

@inbook{
author = "Kolarević, Stoimir and Kracun-Kolarević, Margareta and Jovanović, J. and Ilić, M. and Paunović, Momir and Kostić-Vuković, Jovana and Martinović, Rajko and Jokanović, S. and Joksimović, D. and Pešić, V. and Kirschner, A.K.T. and Linke, R. and Ixenmaier, S. and Farnleitner, Andreas and Savio, D. and Reischer, Georg and Tomić, N. and Vukovic-Gacic, Branka",
year = "2020",
abstract = "The literature data on the microbiological water quality of the surface and groundwaters in Montenegro is very scarce. Therefore, this chapter aims to provide an insight in the microbiological water quality of rivers in Montenegro by compiling the data obtained in period 2009–2018 in national monitoring program and the data collected within the Montenegro survey in 2019 with an emphasis on the hotspots of faecal pollution and possible sources of pollution. Despite the high risk that poor implementation of wastewater treatment might represent for Montenegro, the obtained dataset indicates that overall microbiological water quality of rivers in Montenegro is quite good. About 80% of the investigated sites have bathing water quality according to Montenegrin legislation. Only four sites were identified as hotspots of faecal pollution where the human source of pollution prevails.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Handbook of Environmental Chemistry",
booktitle = "Microbiological water quality of rivers in Montenegro",
pages = "155-135",
volume = "93",
doi = "10.1007/698_2019_420"
}

Kolarević, S., Kracun-Kolarević, M., Jovanović, J., Ilić, M., Paunović, M., Kostić-Vuković, J., Martinović, R., Jokanović, S., Joksimović, D., Pešić, V., Kirschner, A.K.T., Linke, R., Ixenmaier, S., Farnleitner, A., Savio, D., Reischer, G., Tomić, N.,& Vukovic-Gacic, B.. (2020). Microbiological water quality of rivers in Montenegro. in Handbook of Environmental Chemistry
Springer Science and Business Media Deutschland GmbH., 93, 135-155.
https://doi.org/10.1007/698_2019_420

Kolarević S, Kracun-Kolarević M, Jovanović J, Ilić M, Paunović M, Kostić-Vuković J, Martinović R, Jokanović S, Joksimović D, Pešić V, Kirschner A, Linke R, Ixenmaier S, Farnleitner A, Savio D, Reischer G, Tomić N, Vukovic-Gacic B. Microbiological water quality of rivers in Montenegro. in Handbook of Environmental Chemistry. 2020;93:135-155.
doi:10.1007/698_2019_420 .

Kolarević, Stoimir, Kracun-Kolarević, Margareta, Jovanović, J., Ilić, M., Paunović, Momir, Kostić-Vuković, Jovana, Martinović, Rajko, Jokanović, S., Joksimović, D., Pešić, V., Kirschner, A.K.T., Linke, R., Ixenmaier, S., Farnleitner, Andreas, Savio, D., Reischer, Georg, Tomić, N., Vukovic-Gacic, Branka, "Microbiological water quality of rivers in Montenegro" in Handbook of Environmental Chemistry, 93 (2020):135-155,
https://doi.org/10.1007/698_2019_420 . .

TY  - CONF
AU  - Gačić, Zoran
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1692
AB  - Untreated urban wastewaters impose high pressure on receiving water bodies. Pollution pressure may triger a cascade of events in exposed organisms, affecting different levels of biological organisation. Fish are a widely used group of bioindicators in ecogenotoxicological
studies, due to their position in the aquatic trophic web and role in human nutrition. In this work, chub (Squalius cephalus) and bream species (Abramis brama, Blicca bjoerkna, Ballerus sapa) were monitored at reservoirs, Uvac and Garasi, and rivers: Dunav, Sava, Pestan, and Beljanica. Analysis of metals and metalloids in liver, gills, gonads, and muscle was performed by ICP-OES and used as a biomarker of accumulation. The single cell gel electrophoresis, comet assay, was used to assess DNA damage level in blood, liver and gills, as a biomarker of exposure.
Histopathological alterations were monitored in liver and gills of breams from the Sava and Danube Rivers, as a biomarker of effect. Analysis of the accumulation of metals and metalloids in different tissues showed a high level of differentiation, as well as significant differences in tissues distribution of elements between the studied sites. The highest concentrations of metals and metalloids were present in gills, liver and gonads, and the lowestin muscle. Uvac showed the highest quality of water which was confirmed by the low values of DNA damage in all tissues compared to the other sites. In chub, blood showed the lowest level of DNA damage compared to liver and gills, while in all bream species DNA damage was the highest in blood cells, following gills and liver. Histopathological analysis of breams showed a higher degree of alterations in liver compared to the gills. This study highlighted the importance of the multiple biomarkers approach in obtaining information about the response of biota to pollution by analyzing changes at different levels of biological organization.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 47th Annual Meeting of EEMGS, Renn, France
T1  - Bioassays in assessment of genotoxicity and toxicity of freshwater bodies
EP  - 73
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1692
ER  - 

@conference{
author = "Gačić, Zoran and Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Višnjić Jeftić, Željka and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2019",
abstract = "Untreated urban wastewaters impose high pressure on receiving water bodies. Pollution pressure may triger a cascade of events in exposed organisms, affecting different levels of biological organisation. Fish are a widely used group of bioindicators in ecogenotoxicological
studies, due to their position in the aquatic trophic web and role in human nutrition. In this work, chub (Squalius cephalus) and bream species (Abramis brama, Blicca bjoerkna, Ballerus sapa) were monitored at reservoirs, Uvac and Garasi, and rivers: Dunav, Sava, Pestan, and Beljanica. Analysis of metals and metalloids in liver, gills, gonads, and muscle was performed by ICP-OES and used as a biomarker of accumulation. The single cell gel electrophoresis, comet assay, was used to assess DNA damage level in blood, liver and gills, as a biomarker of exposure.
Histopathological alterations were monitored in liver and gills of breams from the Sava and Danube Rivers, as a biomarker of effect. Analysis of the accumulation of metals and metalloids in different tissues showed a high level of differentiation, as well as significant differences in tissues distribution of elements between the studied sites. The highest concentrations of metals and metalloids were present in gills, liver and gonads, and the lowestin muscle. Uvac showed the highest quality of water which was confirmed by the low values of DNA damage in all tissues compared to the other sites. In chub, blood showed the lowest level of DNA damage compared to liver and gills, while in all bream species DNA damage was the highest in blood cells, following gills and liver. Histopathological analysis of breams showed a higher degree of alterations in liver compared to the gills. This study highlighted the importance of the multiple biomarkers approach in obtaining information about the response of biota to pollution by analyzing changes at different levels of biological organization.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "47th Annual Meeting of EEMGS, Renn, France",
title = "Bioassays in assessment of genotoxicity and toxicity of freshwater bodies",
pages = "73-72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1692"
}

Gačić, Z., Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Sunjog, K., Višnjić Jeftić, Ž., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2019). Bioassays in assessment of genotoxicity and toxicity of freshwater bodies. in 47th Annual Meeting of EEMGS, Renn, France
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 72-73.
https://hdl.handle.net/21.15107/rcub_rimsi_1692

Gačić Z, Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Sunjog K, Višnjić Jeftić Ž, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. Bioassays in assessment of genotoxicity and toxicity of freshwater bodies. in 47th Annual Meeting of EEMGS, Renn, France. 2019;:72-73.
https://hdl.handle.net/21.15107/rcub_rimsi_1692 .

Gačić, Zoran, Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Višnjić Jeftić, Željka, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Bioassays in assessment of genotoxicity and toxicity of freshwater bodies" in 47th Annual Meeting of EEMGS, Renn, France (2019):72-73,
https://hdl.handle.net/21.15107/rcub_rimsi_1692 .

TY  - JOUR
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1278
AB  - Due to the increasing industrialization, metals are discharged into all spheres of the environment, in particular, in river courses, which leads to the need for constant monitoring. Metals do not degrade into harmless end products; they are very persistent and have high potential for accumulation in biota. Metals in the fish body are accumulated in different amounts in the tissue specific matter. In relation to the biotic conditions and fish metabolism, the load of food, or the stage of the reproductive cycle, the seasonal variation of metal levels can be expected. Because of that, the objective of our present study was to analyze 15 metals and metalloids in liver, gills, muscle, and gonads of European chub (Squalius cephalus) throughout the 4 seasons, autumn, winter, spring, and summer. The specimens were collected from two rivers, Pestan and Beljanica at the Kolubara basin, and their concentrations were determined with inductively coupled plasma optical emission spectrometry (ICP-OES). Specimens from both rivers have shown similarities in metal accumulation like the highest accumulation of majority of elements in gills, lowest accumulation of majority of elements in muscle (except for Hg), and higher accumulation of some elements in summer (Cu, Fe, Zn). In addition, Cu and Fe showed affinity for liver, while Ba, Cr, Sr, and Zn were specific for gills. Also, Al, B, Fe, Ni, and Pb did not show significant differences in concentrations among different seasons in all investigated tissues.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.)
EP  - 9243
IS  - 9
SP  - 9232
VL  - 26
DO  - 10.1007/s11356-019-04274-3
ER  - 

@article{
author = "Sunjog, Karolina and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Višnjić Jeftić, Željka and Gačić, Zoran and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2019",
abstract = "Due to the increasing industrialization, metals are discharged into all spheres of the environment, in particular, in river courses, which leads to the need for constant monitoring. Metals do not degrade into harmless end products; they are very persistent and have high potential for accumulation in biota. Metals in the fish body are accumulated in different amounts in the tissue specific matter. In relation to the biotic conditions and fish metabolism, the load of food, or the stage of the reproductive cycle, the seasonal variation of metal levels can be expected. Because of that, the objective of our present study was to analyze 15 metals and metalloids in liver, gills, muscle, and gonads of European chub (Squalius cephalus) throughout the 4 seasons, autumn, winter, spring, and summer. The specimens were collected from two rivers, Pestan and Beljanica at the Kolubara basin, and their concentrations were determined with inductively coupled plasma optical emission spectrometry (ICP-OES). Specimens from both rivers have shown similarities in metal accumulation like the highest accumulation of majority of elements in gills, lowest accumulation of majority of elements in muscle (except for Hg), and higher accumulation of some elements in summer (Cu, Fe, Zn). In addition, Cu and Fe showed affinity for liver, while Ba, Cr, Sr, and Zn were specific for gills. Also, Al, B, Fe, Ni, and Pb did not show significant differences in concentrations among different seasons in all investigated tissues.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.)",
pages = "9243-9232",
number = "9",
volume = "26",
doi = "10.1007/s11356-019-04274-3"
}

Sunjog, K., Kolarević, S., Kracun-Kolarević, M., Višnjić Jeftić, Ž., Gačić, Z., Lenhardt, M.,& Vukovic-Gacic, B.. (2019). Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.). in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 26(9), 9232-9243.
https://doi.org/10.1007/s11356-019-04274-3

Sunjog K, Kolarević S, Kracun-Kolarević M, Višnjić Jeftić Ž, Gačić Z, Lenhardt M, Vukovic-Gacic B. Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.). in Environmental Science and Pollution Research. 2019;26(9):9232-9243.
doi:10.1007/s11356-019-04274-3 .

Sunjog, Karolina, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Višnjić Jeftić, Željka, Gačić, Zoran, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.)" in Environmental Science and Pollution Research, 26, no. 9 (2019):9232-9243,
https://doi.org/10.1007/s11356-019-04274-3 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Milovanović, Dragana
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Martinović, Rajko
AU  - Đorđević Aleksić, Jelena
AU  - Novaković, Irena
AU  - Sladic, Dusan
AU  - Vukovic-Gacic, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1274
AB  - In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3 '-methoxyavarone, 4 '-(methylamino)avarone and 3 '-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3 '-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3 '-methoxyavarone and 3 '-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Drug and Chemical Toxicology
T1  - Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models
EP  - 139
IS  - 2
SP  - 130
VL  - 42
DO  - 10.1080/01480545.2017.1413108
ER  - 

@article{
author = "Kolarević, Stoimir and Milovanović, Dragana and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Sunjog, Karolina and Martinović, Rajko and Đorđević Aleksić, Jelena and Novaković, Irena and Sladic, Dusan and Vukovic-Gacic, Branka",
year = "2019",
abstract = "In this study, mutagenic and genotoxic potential of anti-tumor compounds avarol, avarone, and its derivatives 3 '-methoxyavarone, 4 '-(methylamino)avarone and 3 '-(methylamino)avarone was evaluated and compared to cytostatics commonly used in chemotherapy (5-fluorouracil, etoposid, and cisplatin). Mutagenic potential of selected hydroquinone and quinones was assessed in prokaryotic model by the SOS/umuC assay in Salmonella typhimurium TA1535/pSK1002. Genotoxic potential was also assessed in eukaryotic models using comet assay in human fetal lung cell line (MRC-5), human adenocarcinoma epithelial cell line (A549), and in human peripheral blood cells (HPBC). The results indicated that avarol and avarone do not exert mutagenic/genotoxic potential. Among the studied avarone derivatives, mutagenic potential was detected by SOS/umuC test for 3 '-(methylamino)avarone, but only after metabolic activation. The results of comet assay indicated that 3 '-methoxyavarone and 3 '-(methylamino)avarone have a significant impact on the level of DNA damage in the MRC-5 cell line. Genotoxic potential was not observed in A549 cells or HPBC probably due to a different uptake rate for the compounds and lower in metabolism rate within these cells.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Drug and Chemical Toxicology",
title = "Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models",
pages = "139-130",
number = "2",
volume = "42",
doi = "10.1080/01480545.2017.1413108"
}

Kolarević, S., Milovanović, D., Kracun-Kolarević, M., Kostić-Vuković, J., Sunjog, K., Martinović, R., Đorđević Aleksić, J., Novaković, I., Sladic, D.,& Vukovic-Gacic, B.. (2019). Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology
Taylor & Francis Ltd, Abingdon., 42(2), 130-139.
https://doi.org/10.1080/01480545.2017.1413108

Kolarević S, Milovanović D, Kracun-Kolarević M, Kostić-Vuković J, Sunjog K, Martinović R, Đorđević Aleksić J, Novaković I, Sladic D, Vukovic-Gacic B. Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models. in Drug and Chemical Toxicology. 2019;42(2):130-139.
doi:10.1080/01480545.2017.1413108 .

Kolarević, Stoimir, Milovanović, Dragana, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Sunjog, Karolina, Martinović, Rajko, Đorđević Aleksić, Jelena, Novaković, Irena, Sladic, Dusan, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential of avarol, avarone, and its methoxy and methylamino derivatives in prokaryotic and eukaryotic test models" in Drug and Chemical Toxicology, 42, no. 2 (2019):130-139,
https://doi.org/10.1080/01480545.2017.1413108 . .

TY  - JOUR
AU  - Sunjog, Karolina
AU  - Cirković, Sanja
AU  - Vukovic-Gacic, Branka
AU  - Guc-Scekic, Marija
AU  - Misković, Marijana
AU  - Vujic, Dragana
AU  - Skoric, Dejan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1217
AB  - Fanconi anemia (FA) is a complex genetic disease with a variety of congenital and hematological symptoms, including the predisposition for cancer development. The main hallmark of FA cells, an increased chromosomal fragility, in the presence of the DNA-interstrand cross-linking chemicals, mitomycin C or diepoxybutane (DEB), makes the diagnosis of FA much easier. Cytogenetic method can detect the FA patients with highly elevated chromosomal breakage, but also some of the patients with borderline sensitivity to DEB no matter if they have FA or not. These particular circumstances lead us to introduce comet assay along with cytogenetic analysis, in order to determine DNA lesions and chromosomal fragility in untreated and DEB-treated lymphocytes of full blood from seven patients with clinical features of FA. Highly elevated DEB induced chromosomal sensitivity confirmed the diagnosis in five patients (FA group: 0.48-4.47 breaks/cell vs control group: 0.00-0.08 breaks/cell). Borderline DEB sensitivity (FA* group: 0.15-0.44 breaks/cell) was found in the remaining two patients. Results of the comet assay showed higher baseline and DEB-induced DNA damage values (Olive tail moment and tail intensity) in all five FA and one FA* patient, when compared to the control group. These findings could provide a new model of FA screening test algorithm, including comet assay as additional and very useful accurate tool, beside the DEB test, in differential diagnosis of FA.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Comet assay and cytogenetic findings in differential diagnosis of fanconi anemia
EP  - 1126
IS  - 3
SP  - 1113
VL  - 51
DO  - 10.2298/GENSR1903113S
ER  - 

@article{
author = "Sunjog, Karolina and Cirković, Sanja and Vukovic-Gacic, Branka and Guc-Scekic, Marija and Misković, Marijana and Vujic, Dragana and Skoric, Dejan",
year = "2019",
abstract = "Fanconi anemia (FA) is a complex genetic disease with a variety of congenital and hematological symptoms, including the predisposition for cancer development. The main hallmark of FA cells, an increased chromosomal fragility, in the presence of the DNA-interstrand cross-linking chemicals, mitomycin C or diepoxybutane (DEB), makes the diagnosis of FA much easier. Cytogenetic method can detect the FA patients with highly elevated chromosomal breakage, but also some of the patients with borderline sensitivity to DEB no matter if they have FA or not. These particular circumstances lead us to introduce comet assay along with cytogenetic analysis, in order to determine DNA lesions and chromosomal fragility in untreated and DEB-treated lymphocytes of full blood from seven patients with clinical features of FA. Highly elevated DEB induced chromosomal sensitivity confirmed the diagnosis in five patients (FA group: 0.48-4.47 breaks/cell vs control group: 0.00-0.08 breaks/cell). Borderline DEB sensitivity (FA* group: 0.15-0.44 breaks/cell) was found in the remaining two patients. Results of the comet assay showed higher baseline and DEB-induced DNA damage values (Olive tail moment and tail intensity) in all five FA and one FA* patient, when compared to the control group. These findings could provide a new model of FA screening test algorithm, including comet assay as additional and very useful accurate tool, beside the DEB test, in differential diagnosis of FA.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Comet assay and cytogenetic findings in differential diagnosis of fanconi anemia",
pages = "1126-1113",
number = "3",
volume = "51",
doi = "10.2298/GENSR1903113S"
}

Sunjog, K., Cirković, S., Vukovic-Gacic, B., Guc-Scekic, M., Misković, M., Vujic, D.,& Skoric, D.. (2019). Comet assay and cytogenetic findings in differential diagnosis of fanconi anemia. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 51(3), 1113-1126.
https://doi.org/10.2298/GENSR1903113S

Sunjog K, Cirković S, Vukovic-Gacic B, Guc-Scekic M, Misković M, Vujic D, Skoric D. Comet assay and cytogenetic findings in differential diagnosis of fanconi anemia. in Genetika-Belgrade. 2019;51(3):1113-1126.
doi:10.2298/GENSR1903113S .

Sunjog, Karolina, Cirković, Sanja, Vukovic-Gacic, Branka, Guc-Scekic, Marija, Misković, Marijana, Vujic, Dragana, Skoric, Dejan, "Comet assay and cytogenetic findings in differential diagnosis of fanconi anemia" in Genetika-Belgrade, 51, no. 3 (2019):1113-1126,
https://doi.org/10.2298/GENSR1903113S . .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Gačić, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1667
AB  - Biomarkers are used in ecosystem health assessment because they provide information about
the biological effects of pollution. Aquatic ecosystems are often the ultimate recipients of
different pollutants, which may have potentially genotoxic and carcinogenic effects on aquatic
organisms.
The aim of this study was to assess the impact of untreated urban wastewater during different
seasons on the DNA damage level and histopathological alterations in gills and liver of common
bream (Abramis brama). This study was carried out at the site Višnjica, situated downstream
from Belgrade, which is identified as one of the major hotspots of fecal pollution along the
Danube River. Sampling was performed in February, April, August and November 2014. DNA
damage in fish tissues was assessed by alkaline comet assay. Histopathological alterations were
quantified by combining pathological significance and the extent for each lesion. Subsequently,
lesion scores within organs are summed in order to calculate histopathological index.
The highest level of DNA damage in both tissues was observed in August. Liver had the lowest
level of DNA damage in February, and gills in April. Gills had a significantly higher level of
DNA damage in comparison to liver during all months, except in April.
Histopathological index of the liver had the highest values in April and of the gills in November.
Both tissues had the lowest value of histopathological index in August. In general,
histopathological alterations in liver were more severe in comparison to gills during all months,
except in November.
The presence of DNA damage acted as an early-marker signal in fish, while histopathological
alterations reflected state of fish organs when exposed to contaminants during long term
pollution. This study confirmed the potential of untreated urban wastewaters to trigger
responses on different levels of biological organization in fish.
PB  - Institute of Geography of SAS
C3  - The 42nd IAD Conference 2018, Smolenice, Slovakia
T1  - DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution
EP  - 34
SP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1667
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Gačić, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "Biomarkers are used in ecosystem health assessment because they provide information about
the biological effects of pollution. Aquatic ecosystems are often the ultimate recipients of
different pollutants, which may have potentially genotoxic and carcinogenic effects on aquatic
organisms.
The aim of this study was to assess the impact of untreated urban wastewater during different
seasons on the DNA damage level and histopathological alterations in gills and liver of common
bream (Abramis brama). This study was carried out at the site Višnjica, situated downstream
from Belgrade, which is identified as one of the major hotspots of fecal pollution along the
Danube River. Sampling was performed in February, April, August and November 2014. DNA
damage in fish tissues was assessed by alkaline comet assay. Histopathological alterations were
quantified by combining pathological significance and the extent for each lesion. Subsequently,
lesion scores within organs are summed in order to calculate histopathological index.
The highest level of DNA damage in both tissues was observed in August. Liver had the lowest
level of DNA damage in February, and gills in April. Gills had a significantly higher level of
DNA damage in comparison to liver during all months, except in April.
Histopathological index of the liver had the highest values in April and of the gills in November.
Both tissues had the lowest value of histopathological index in August. In general,
histopathological alterations in liver were more severe in comparison to gills during all months,
except in November.
The presence of DNA damage acted as an early-marker signal in fish, while histopathological
alterations reflected state of fish organs when exposed to contaminants during long term
pollution. This study confirmed the potential of untreated urban wastewaters to trigger
responses on different levels of biological organization in fish.",
publisher = "Institute of Geography of SAS",
journal = "The 42nd IAD Conference 2018, Smolenice, Slovakia",
title = "DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution",
pages = "34-34",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1667"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Gačić, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2018). DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution. in The 42nd IAD Conference 2018, Smolenice, Slovakia
Institute of Geography of SAS., 34-34.
https://hdl.handle.net/21.15107/rcub_rimsi_1667

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Gačić Z, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution. in The 42nd IAD Conference 2018, Smolenice, Slovakia. 2018;:34-34.
https://hdl.handle.net/21.15107/rcub_rimsi_1667 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Gačić, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "DNA damage and histopathological alterations in liver and gills of common bream Abramis brama (L.) as biomarkers of the Danube River pollution" in The 42nd IAD Conference 2018, Smolenice, Slovakia (2018):34-34,
https://hdl.handle.net/21.15107/rcub_rimsi_1667 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Gačić, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1681
AB  - The Danube, the second largest river in Europe, is of great importance for all the countries in the basin for production of drinking water, water supply for domestic, agricultural and industrial purposes. Along the stream, the river receives incompletely treated or untreated wastewaters, which deteriorates its quality. Measurements of physico-chemical, chemical and microbiological parameters are considered the basis of monitoring because they cover a wide spectrum of information for proper water management. However, aquatic ecosystems are often exposed to different pollutants which may exhibit harmful effects on different levels of biological organization. Fish are widely used bioindicators in ecogenotoxicological studies, because of their position in the aquatic trophic chain and importance in human diet.
This research was conducted on the Danube River locality Višnjica, situated on the right river bank (1162 rkm), one of the main hotspots of fecal pollution. Discharge of the largest waste water collector in the capital of Serbia, Belgrade, which receives domestic and industrial waste, is situated a few km upstream of the sampling site. Sampling was performed in February, April, August and November 2014, with the aim to evaluate the impact of seasonal variation on water quality parameters and response of biomarkers in the common bream (Abramis brama). Water quality was assessed by using basic physico-chemical parameters and microbiological indicators of fecal pollution. In gills and liver of bream, metal and metalloid concentrations were assessed as an accumulation biomarker, comet assay was applied to assess DNA damage as a biomarker of exposure, while histopathological analysis was performed as a biomarker of effect.
According to number of Escherichia coli and enterococci critical and excessive level of fecal pollution was present on the site, indicating the presence of high amounts of untreated waste waters. During the entire period gills had higher concentrations of metals and metalloids in comparison to the liver. Both tissues showed the highest metal accumulation and the highest level of DNA damage in August, which may be attributed to increased activity and feeding during summer, but also to low flow rate and water level which may increase pollutant concentrations in the river. The highest level of histopathological alterations in the gills was recorded in November and in liver in April, probably due to higher level of precipitation during these months and introduction of pollutants through the urban and agricultural runoff. In terms of elements concentrations and DNA damage gills were more affected, while in terms of histopathological alterations liver had higher level of histopathological alterations. These results showed that urban wastewaters have a high potential in inducing genotoxic and histopathological effects in fish, and highlighted the urgent need for implementation of wastewater treatment facilities.
PB  - University of Agriculture in Krakow
C3  - Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
T1  - The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758)
EP  - 162
SP  - 162
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1681
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Gačić, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The Danube, the second largest river in Europe, is of great importance for all the countries in the basin for production of drinking water, water supply for domestic, agricultural and industrial purposes. Along the stream, the river receives incompletely treated or untreated wastewaters, which deteriorates its quality. Measurements of physico-chemical, chemical and microbiological parameters are considered the basis of monitoring because they cover a wide spectrum of information for proper water management. However, aquatic ecosystems are often exposed to different pollutants which may exhibit harmful effects on different levels of biological organization. Fish are widely used bioindicators in ecogenotoxicological studies, because of their position in the aquatic trophic chain and importance in human diet.
This research was conducted on the Danube River locality Višnjica, situated on the right river bank (1162 rkm), one of the main hotspots of fecal pollution. Discharge of the largest waste water collector in the capital of Serbia, Belgrade, which receives domestic and industrial waste, is situated a few km upstream of the sampling site. Sampling was performed in February, April, August and November 2014, with the aim to evaluate the impact of seasonal variation on water quality parameters and response of biomarkers in the common bream (Abramis brama). Water quality was assessed by using basic physico-chemical parameters and microbiological indicators of fecal pollution. In gills and liver of bream, metal and metalloid concentrations were assessed as an accumulation biomarker, comet assay was applied to assess DNA damage as a biomarker of exposure, while histopathological analysis was performed as a biomarker of effect.
According to number of Escherichia coli and enterococci critical and excessive level of fecal pollution was present on the site, indicating the presence of high amounts of untreated waste waters. During the entire period gills had higher concentrations of metals and metalloids in comparison to the liver. Both tissues showed the highest metal accumulation and the highest level of DNA damage in August, which may be attributed to increased activity and feeding during summer, but also to low flow rate and water level which may increase pollutant concentrations in the river. The highest level of histopathological alterations in the gills was recorded in November and in liver in April, probably due to higher level of precipitation during these months and introduction of pollutants through the urban and agricultural runoff. In terms of elements concentrations and DNA damage gills were more affected, while in terms of histopathological alterations liver had higher level of histopathological alterations. These results showed that urban wastewaters have a high potential in inducing genotoxic and histopathological effects in fish, and highlighted the urgent need for implementation of wastewater treatment facilities.",
publisher = "University of Agriculture in Krakow",
journal = "Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland",
title = "The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758)",
pages = "162-162",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1681"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Gačić, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2018). The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758). in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland
University of Agriculture in Krakow., 162-162.
https://hdl.handle.net/21.15107/rcub_rimsi_1681

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Gačić Z, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758). in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland. 2018;:162-162.
https://hdl.handle.net/21.15107/rcub_rimsi_1681 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Gačić, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "The impact of the Danube River pollution on biomarkers response in the liver and gills of common bream Abramis brama (L., 1758)" in Central and Eastern European Conference on Health and Environment CEECHE 2018, Krakow, Poland (2018):162-162,
https://hdl.handle.net/21.15107/rcub_rimsi_1681 .

TY  - JOUR
AU  - Jovanović, Jovana
AU  - Kolarević, Stoimir
AU  - Milosković, Aleksandra
AU  - Radojković, Nataša
AU  - Simic, Vladica
AU  - Dojčinović, Biljana
AU  - Kracun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Timilijic, Jovana
AU  - Đorđević Aleksić, Jelena
AU  - Gačić, Zoran
AU  - Zegura, Bojana
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1163
AB  - The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ
EP  - 1299
SP  - 1289
VL  - 621
DO  - 10.1016/j.scitotenv.2017.10.099
ER  - 

@article{
author = "Jovanović, Jovana and Kolarević, Stoimir and Milosković, Aleksandra and Radojković, Nataša and Simic, Vladica and Dojčinović, Biljana and Kracun-Kolarević, Margareta and Paunović, Momir and Kostić-Vuković, Jovana and Sunjog, Karolina and Timilijic, Jovana and Đorđević Aleksić, Jelena and Gačić, Zoran and Zegura, Bojana and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The Velika Morava River is the greatest national Serbian river and the significant tributary of the Danube River. The major problems in the Velika Morava River Basin (VMRB) represent untreated industrial and municipal wastewaters. In this study, the level of genotoxic potential at the sites along the VMRB was evaluated by parallel in vitro and in situ approach. Within in vitro testing, genotoxicity of native water samples collected from the sites in VMRB was evaluated by SOS/umuC test on Salmonella typhimurium TA1535/pSK1002 and by the comet assay on HepG2 cells. DNA damage in situ was assessed in bleak (Alburnus alburnus) erythrocytes by the comet (alkaline and Fpg-modified comet) and micronucleus assays. Additionally, the concentration of heavy metals in fish tissue was measured and this data, compiled with the data of the physico-chemical parameters measured in water, was used as a measure of the pollution pressure at the sites. Results showed that applied in vitro tests with native water samples are less sensitive in comparison with in situ tests and should be taken with precaution when making predictions on the status of the ecosystem. Within applied battery of in situ assays differential sensitivity of assays was observed where alkaline comet assay showed the highest potential in differentiation of the sites based on genotoxic potential. Integrated biomarker response showed that usage of the battery of bioassays provides better insight in a genotoxic effects in animals, and consequently, that the holistic approach is more suitable for this type of study.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ",
pages = "1299-1289",
volume = "621",
doi = "10.1016/j.scitotenv.2017.10.099"
}

Jovanović, J., Kolarević, S., Milosković, A., Radojković, N., Simic, V., Dojčinović, B., Kracun-Kolarević, M., Paunović, M., Kostić-Vuković, J., Sunjog, K., Timilijic, J., Đorđević Aleksić, J., Gačić, Z., Zegura, B.,& Vukovic-Gacic, B.. (2018). Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 621, 1289-1299.
https://doi.org/10.1016/j.scitotenv.2017.10.099

Jovanović J, Kolarević S, Milosković A, Radojković N, Simic V, Dojčinović B, Kracun-Kolarević M, Paunović M, Kostić-Vuković J, Sunjog K, Timilijic J, Đorđević Aleksić J, Gačić Z, Zegura B, Vukovic-Gacic B. Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ. in Science of the Total Environment. 2018;621:1289-1299.
doi:10.1016/j.scitotenv.2017.10.099 .

Jovanović, Jovana, Kolarević, Stoimir, Milosković, Aleksandra, Radojković, Nataša, Simic, Vladica, Dojčinović, Biljana, Kracun-Kolarević, Margareta, Paunović, Momir, Kostić-Vuković, Jovana, Sunjog, Karolina, Timilijic, Jovana, Đorđević Aleksić, Jelena, Gačić, Zoran, Zegura, Bojana, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential in the Velika Morava River Basin in vitro and in situ" in Science of the Total Environment, 621 (2018):1289-1299,
https://doi.org/10.1016/j.scitotenv.2017.10.099 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Jovanović, Jovana
AU  - Simonović, Predrag
AU  - Simic, Vladica
AU  - Piria, Marina
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1160
AB  - The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis. [GRAPHICS] .
PB  - Springer International Publishing Ag, Cham
T2  - International Journal of Environmental Research
T1  - The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study
EP  - 712
IS  - 5
SP  - 703
VL  - 12
DO  - 10.1007/s41742-018-0127-6
ER  - 

@article{
author = "Kolarević, Stoimir and Sunjog, Karolina and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Jovanović, Jovana and Simonović, Predrag and Simic, Vladica and Piria, Marina and Gačić, Zoran and Lenhardt, Mirjana and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2018",
abstract = "The major objective of this study was to investigate the impact of wastewaters on the level of genotoxicity and the possible reflection of genotoxicity on the genetic variability in chub (Squalius cephalus) collected from the sites under different pollution pressures along the Sava River. To emphasize the impact of wastewaters, this study was carried out in a season with low water discharge. The level of genotoxic potential at the sites was studied by alkaline and Fpg-modified comet assay in fish blood, while RAPD technique was employed on the fish muscle tissue. The variations in the genotoxic potential were observed along the Sava River based on the level of DNA damage in blood of the specimens collected at the selected sites. Clustering of the impacted sites was also observed based on RAPD fingerprints. Comparison of the data obtained in RAPD and the data from the comet assay indicated the possible reflection of genotoxicity on the genetic variability in chub collected from the sites with different pollution pressure. This is a preliminary study which reports the differentiation of the population of chub along the Sava River based on RAPD analysis. [GRAPHICS] .",
publisher = "Springer International Publishing Ag, Cham",
journal = "International Journal of Environmental Research",
title = "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study",
pages = "712-703",
number = "5",
volume = "12",
doi = "10.1007/s41742-018-0127-6"
}

Kolarević, S., Sunjog, K., Kracun-Kolarević, M., Kostić-Vuković, J., Jovanović, J., Simonović, P., Simic, V., Piria, M., Gačić, Z., Lenhardt, M., Paunović, M.,& Vukovic-Gacic, B.. (2018). The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research
Springer International Publishing Ag, Cham., 12(5), 703-712.
https://doi.org/10.1007/s41742-018-0127-6

Kolarević S, Sunjog K, Kracun-Kolarević M, Kostić-Vuković J, Jovanović J, Simonović P, Simic V, Piria M, Gačić Z, Lenhardt M, Paunović M, Vukovic-Gacic B. The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study. in International Journal of Environmental Research. 2018;12(5):703-712.
doi:10.1007/s41742-018-0127-6 .

Kolarević, Stoimir, Sunjog, Karolina, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Jovanović, Jovana, Simonović, Predrag, Simic, Vladica, Piria, Marina, Gačić, Zoran, Lenhardt, Mirjana, Paunović, Momir, Vukovic-Gacic, Branka, "The Genetic Variability (RAPD) and Genotoxicity In Vivo (Alkaline and Fpg-Modified Comet Assay) in Chub (Squalius cephalus): The Sava River Case Study" in International Journal of Environmental Research, 12, no. 5 (2018):703-712,
https://doi.org/10.1007/s41742-018-0127-6 . .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Gačić, Zoran
AU  - Paunović, Momir
AU  - Višnjić Jeftić, Željka
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2017
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1060
AB  - Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons
EP  - 1681
SP  - 1670
VL  - 601
DO  - 10.1016/j.scitotenv.2017.05.273
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Aborgiba, Mustafa and Gačić, Zoran and Paunović, Momir and Višnjić Jeftić, Željka and Rasković, Božidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2017",
abstract = "Biomarkers attract increasing attention in environmental studies, as a tool for detection of exposure and effects of pollution, from both natural and anthropogenic sources. This study aims to assess the impact of multiple stressors during distinctive seasons, covering also extreme hydrological events (extensive flooding in the mid May 2014), on different levels of biological organization in the liver and gills of three closely related freshwater breams. Our previous study on DNA damage in blood cells of these specimens showed increased DNA damage in June 2014, one month after the flooding event. As a continuation of that research, the present study was conducted. As a biomarker of exposure DNA damage was measured by applying the alkaline comet assay, while histopathological alterations were monitored as a biomarker of effect. Additionally, concentrations of metals and metalloids in gills, liver and muscle were assessed. Sampling of fish tissues was performed in 2014, during winter (January and February), spring (March and early June) and summer (late June, July and August). Significant seasonal difference in DNA damage was observed for both tissues. During spring and summer the level of DNA damage in gills was significantly higher when compared to the liver. Histopathological analyses showed higher frequency of alterations in gills during spring, and in liver during summer, but without a significant seasonal difference. Gills had the highest concentration of metals and metalloids during the spring and summer, and liver duringwinter. Muscle was the least affected tissue during all three seasons. This study highlighted the importance of the multiple biomarker approach and the use of different fish tissues in assessment of surface water pollution.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons",
pages = "1681-1670",
volume = "601",
doi = "10.1016/j.scitotenv.2017.05.273"
}

Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Aborgiba, M., Gačić, Z., Paunović, M., Višnjić Jeftić, Ž., Rasković, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2017). The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 601, 1670-1681.
https://doi.org/10.1016/j.scitotenv.2017.05.273

Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Aborgiba M, Gačić Z, Paunović M, Višnjić Jeftić Ž, Rasković B, Poleksic V, Lenhardt M, Vukovic-Gacic B. The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons. in Science of the Total Environment. 2017;601:1670-1681.
doi:10.1016/j.scitotenv.2017.05.273 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Aborgiba, Mustafa, Gačić, Zoran, Paunović, Momir, Višnjić Jeftić, Željka, Rasković, Božidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "The impact of multiple stressors on the biomarkers response in gills and liver of freshwater breams during different seasons" in Science of the Total Environment, 601 (2017):1670-1681,
https://doi.org/10.1016/j.scitotenv.2017.05.273 . .

TY  - JOUR
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Višnjić Jeftić, Željka
AU  - Skorić, Stefan
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vasic, Nebojša
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/951
AB  - Metals and metalloids are natural components of the biosphere, which are not produced per se by human beings, but whose form and distribution can be affected by human activities. Like all substances, they are a contaminant if present in excess compared to background levels and/or in a form that would not normally occur in the environment. Samples of liver, gills, gonads and muscle from European chub, Squalius cephalus, were analyzed for Al, As, B, Ba, Cr, Cu, Fe, Hg, Mn, Mo, Sr and Zn using inductively coupled plasma optical emission spectrometry (ICP-OES) to highlight the importance of tissue selection in monitoring research. The comet assay or single cell gel electrophoresis (SCGE) was selected as an in vivo genotoxicity assay, a rapid and sensitive method for measuring genotoxic effects in blood, liver and gills of the European chub. Microscopic images of comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). The objective of our study was to investigate two reservoirs, Zlatar and Garasi, and one river, Pestan by: (i) determining and comparing metal and metalloid concentrations in sediment, water and tissues of European chub: liver, gills, muscle and gonads (ii) comparing these findings with genotoxicity of water expressed through DNA damage of fish tissues. A clear link between the level of metals in water, sediment and tissues and between metal and genotoxicity levels at examined sites was not found. This suggests that other xenobiotics (possibly the organic compounds), contribute to DNA damage.
PB  - Elsevier Sci Ltd, Oxford
T2  - Environmental Pollution
T1  - Assessment of status of three water bodies in Serbia based on tissue metal and metalloid concentration (ICP-OES) and genotoxicity (comet assay)
EP  - 607
SP  - 600
VL  - 213
DO  - 10.1016/j.envpol.2016.03.008
ER  - 

@article{
author = "Sunjog, Karolina and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Višnjić Jeftić, Željka and Skorić, Stefan and Gačić, Zoran and Lenhardt, Mirjana and Vasic, Nebojša and Vukovic-Gacic, Branka",
year = "2016",
abstract = "Metals and metalloids are natural components of the biosphere, which are not produced per se by human beings, but whose form and distribution can be affected by human activities. Like all substances, they are a contaminant if present in excess compared to background levels and/or in a form that would not normally occur in the environment. Samples of liver, gills, gonads and muscle from European chub, Squalius cephalus, were analyzed for Al, As, B, Ba, Cr, Cu, Fe, Hg, Mn, Mo, Sr and Zn using inductively coupled plasma optical emission spectrometry (ICP-OES) to highlight the importance of tissue selection in monitoring research. The comet assay or single cell gel electrophoresis (SCGE) was selected as an in vivo genotoxicity assay, a rapid and sensitive method for measuring genotoxic effects in blood, liver and gills of the European chub. Microscopic images of comets were scored using Comet IV Computer Software (Perceptive Instruments, UK). The objective of our study was to investigate two reservoirs, Zlatar and Garasi, and one river, Pestan by: (i) determining and comparing metal and metalloid concentrations in sediment, water and tissues of European chub: liver, gills, muscle and gonads (ii) comparing these findings with genotoxicity of water expressed through DNA damage of fish tissues. A clear link between the level of metals in water, sediment and tissues and between metal and genotoxicity levels at examined sites was not found. This suggests that other xenobiotics (possibly the organic compounds), contribute to DNA damage.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Environmental Pollution",
title = "Assessment of status of three water bodies in Serbia based on tissue metal and metalloid concentration (ICP-OES) and genotoxicity (comet assay)",
pages = "607-600",
volume = "213",
doi = "10.1016/j.envpol.2016.03.008"
}

Sunjog, K., Kolarević, S., Kracun-Kolarević, M., Višnjić Jeftić, Ž., Skorić, S., Gačić, Z., Lenhardt, M., Vasic, N.,& Vukovic-Gacic, B.. (2016). Assessment of status of three water bodies in Serbia based on tissue metal and metalloid concentration (ICP-OES) and genotoxicity (comet assay). in Environmental Pollution
Elsevier Sci Ltd, Oxford., 213, 600-607.
https://doi.org/10.1016/j.envpol.2016.03.008

Sunjog K, Kolarević S, Kracun-Kolarević M, Višnjić Jeftić Ž, Skorić S, Gačić Z, Lenhardt M, Vasic N, Vukovic-Gacic B. Assessment of status of three water bodies in Serbia based on tissue metal and metalloid concentration (ICP-OES) and genotoxicity (comet assay). in Environmental Pollution. 2016;213:600-607.
doi:10.1016/j.envpol.2016.03.008 .

Sunjog, Karolina, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Višnjić Jeftić, Željka, Skorić, Stefan, Gačić, Zoran, Lenhardt, Mirjana, Vasic, Nebojša, Vukovic-Gacic, Branka, "Assessment of status of three water bodies in Serbia based on tissue metal and metalloid concentration (ICP-OES) and genotoxicity (comet assay)" in Environmental Pollution, 213 (2016):600-607,
https://doi.org/10.1016/j.envpol.2016.03.008 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Gačić, Zoran
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kracun-Kolarević, Margareta
AU  - Paunović, Momir
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1014
AB  - The impact of etoposide (ETO), vincristine sulfate (VIN), and cisplatin (CP) on the DNA damage level was studied in vivo and in vitro on hemocytes of freshwater mussels Unio sp. (Unio pictorum/Unio tumidus) using the alkaline comet assay. For in vivo experiments, the mussels were exposed in static system for 72 h. For in vitro experiments, treatment was performed in primary cultures of hemocytes for 22 h. The DNA damage level was analyzed by Comet Assay IV software. Increase of damage was detected for ETO (40 and 100 mu M) in vivo and in vitro and for VIN (0.04 and 0.1 mu M) in vivo. CP did not induce an increase of DNA damage, but post-treatment with hydrogen peroxide indicated existence of DNA crosslinks in specimens treated with 4 mu M CP. Effective concentrations of selected cytostatics are at least one (CP) or several orders of magnitude (ETO, VIN) higher comparing to the concentrations in hospital wastewater.
PB  - Wiley, Hoboken
T2  - Clean-Soil Air Water
T1  - Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus
EP  - 1476
IS  - 11
SP  - 1471
VL  - 44
DO  - 10.1002/clen.201500482
ER  - 

@article{
author = "Kolarević, Stoimir and Gačić, Zoran and Kostić-Vuković, Jovana and Sunjog, Karolina and Kracun-Kolarević, Margareta and Paunović, Momir and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka",
year = "2016",
abstract = "The impact of etoposide (ETO), vincristine sulfate (VIN), and cisplatin (CP) on the DNA damage level was studied in vivo and in vitro on hemocytes of freshwater mussels Unio sp. (Unio pictorum/Unio tumidus) using the alkaline comet assay. For in vivo experiments, the mussels were exposed in static system for 72 h. For in vitro experiments, treatment was performed in primary cultures of hemocytes for 22 h. The DNA damage level was analyzed by Comet Assay IV software. Increase of damage was detected for ETO (40 and 100 mu M) in vivo and in vitro and for VIN (0.04 and 0.1 mu M) in vivo. CP did not induce an increase of DNA damage, but post-treatment with hydrogen peroxide indicated existence of DNA crosslinks in specimens treated with 4 mu M CP. Effective concentrations of selected cytostatics are at least one (CP) or several orders of magnitude (ETO, VIN) higher comparing to the concentrations in hospital wastewater.",
publisher = "Wiley, Hoboken",
journal = "Clean-Soil Air Water",
title = "Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus",
pages = "1476-1471",
number = "11",
volume = "44",
doi = "10.1002/clen.201500482"
}

Kolarević, S., Gačić, Z., Kostić-Vuković, J., Sunjog, K., Kracun-Kolarević, M., Paunović, M., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2016). Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus. in Clean-Soil Air Water
Wiley, Hoboken., 44(11), 1471-1476.
https://doi.org/10.1002/clen.201500482

Kolarević S, Gačić Z, Kostić-Vuković J, Sunjog K, Kracun-Kolarević M, Paunović M, Knezevic-Vukcević J, Vukovic-Gacic B. Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus. in Clean-Soil Air Water. 2016;44(11):1471-1476.
doi:10.1002/clen.201500482 .

Kolarević, Stoimir, Gačić, Zoran, Kostić-Vuković, Jovana, Sunjog, Karolina, Kracun-Kolarević, Margareta, Paunović, Momir, Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "Impact of Common Cytostatics on DNA Damage in Freshwater Mussels Unio pictorum and Unio tumidus" in Clean-Soil Air Water, 44, no. 11 (2016):1471-1476,
https://doi.org/10.1002/clen.201500482 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Milovanović, D.
AU  - Avdović, M.
AU  - Oalde, Mariana
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Nikolić, B.
AU  - Knezevic-Vukcević, Jelena
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/973
AB  - In this study we investigated the influence of preparation of the bacterial inoculum for a microdilution susceptibility test, e.g., the effect of its optical density, on assessment of the minimum inhibitory concentrations (MIC). The approach employed in the majority of microdilution susceptibility studies is use of the same optical density for preparation of inoculums for different bacterial strains. In the present work, this approach was questioned by determining the ratio between the optical density and the number of bacteria in cultures. We also investigated whether the number of bacteria in inoculums can affect assessment of the MIC value for two antibiotics of broad spectra, rifampicin and streptomycin. The study was performed on four Gram-positive and four Gram-negative bacteria (ATCC collection) commonly used to investigate antimicrobial potential. The ratio between the optical density and number of bacteria in cultures was determined for each strain, and a strong linear correlation was detected. However, it was evident that different bacteria have different cell numbers at the same OD600 value. Based on the obtained results, inoculums for selected strains were prepared to obtain final cell numbers of 103, 104, 105 and 106 /well in the microdilution assay. Two different approaches were used in determining the MIC for rifampicin and streptomycin: approximation of MIC with IC90 and the resazurin reduction assay. Our results indicated that the ratio between optical density and cell numbers is not constant and use of the same OD for inoculums for all strains can therefore lead to misinterpretation of the MIC values. We also observed influence of cell numbers in inoculums in determination of MIC values. For both approaches used (approximation of MIC with IC90 and the resazurin reduction assay), the same trend was detected: antibiotics had the highest potency in experiments with the lowest bacteria cell number (103/well). The lowest cell number (103/well) is not recommended, as it can lead to false susceptibility results and to partial reduction of resazurin, which further complicates MIC determination. A final cell number of 104/well can therefore be recommended as optimal.
PB  - Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd
T2  - Botanica Serbica
T1  - Optimisation of the microdilution method for detection of minimum inhibitory concentration values in selected bacteria
EP  - 36
IS  - 1
SP  - 29
VL  - 40
DO  - 10.5281/zenodo.48751
ER  - 

@article{
author = "Kolarević, Stoimir and Milovanović, D. and Avdović, M. and Oalde, Mariana and Kostić-Vuković, Jovana and Sunjog, Karolina and Nikolić, B. and Knezevic-Vukcević, Jelena and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study we investigated the influence of preparation of the bacterial inoculum for a microdilution susceptibility test, e.g., the effect of its optical density, on assessment of the minimum inhibitory concentrations (MIC). The approach employed in the majority of microdilution susceptibility studies is use of the same optical density for preparation of inoculums for different bacterial strains. In the present work, this approach was questioned by determining the ratio between the optical density and the number of bacteria in cultures. We also investigated whether the number of bacteria in inoculums can affect assessment of the MIC value for two antibiotics of broad spectra, rifampicin and streptomycin. The study was performed on four Gram-positive and four Gram-negative bacteria (ATCC collection) commonly used to investigate antimicrobial potential. The ratio between the optical density and number of bacteria in cultures was determined for each strain, and a strong linear correlation was detected. However, it was evident that different bacteria have different cell numbers at the same OD600 value. Based on the obtained results, inoculums for selected strains were prepared to obtain final cell numbers of 103, 104, 105 and 106 /well in the microdilution assay. Two different approaches were used in determining the MIC for rifampicin and streptomycin: approximation of MIC with IC90 and the resazurin reduction assay. Our results indicated that the ratio between optical density and cell numbers is not constant and use of the same OD for inoculums for all strains can therefore lead to misinterpretation of the MIC values. We also observed influence of cell numbers in inoculums in determination of MIC values. For both approaches used (approximation of MIC with IC90 and the resazurin reduction assay), the same trend was detected: antibiotics had the highest potency in experiments with the lowest bacteria cell number (103/well). The lowest cell number (103/well) is not recommended, as it can lead to false susceptibility results and to partial reduction of resazurin, which further complicates MIC determination. A final cell number of 104/well can therefore be recommended as optimal.",
publisher = "Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd",
journal = "Botanica Serbica",
title = "Optimisation of the microdilution method for detection of minimum inhibitory concentration values in selected bacteria",
pages = "36-29",
number = "1",
volume = "40",
doi = "10.5281/zenodo.48751"
}

Kolarević, S., Milovanović, D., Avdović, M., Oalde, M., Kostić-Vuković, J., Sunjog, K., Nikolić, B., Knezevic-Vukcević, J.,& Vukovic-Gacic, B.. (2016). Optimisation of the microdilution method for detection of minimum inhibitory concentration values in selected bacteria. in Botanica Serbica
Univerzitet u Beogradu - Biološki fakultet - Institut za botaniku i botaničku baštu "Jevremovac", Beograd., 40(1), 29-36.
https://doi.org/10.5281/zenodo.48751

Kolarević S, Milovanović D, Avdović M, Oalde M, Kostić-Vuković J, Sunjog K, Nikolić B, Knezevic-Vukcević J, Vukovic-Gacic B. Optimisation of the microdilution method for detection of minimum inhibitory concentration values in selected bacteria. in Botanica Serbica. 2016;40(1):29-36.
doi:10.5281/zenodo.48751 .

Kolarević, Stoimir, Milovanović, D., Avdović, M., Oalde, Mariana, Kostić-Vuković, Jovana, Sunjog, Karolina, Nikolić, B., Knezevic-Vukcević, Jelena, Vukovic-Gacic, Branka, "Optimisation of the microdilution method for detection of minimum inhibitory concentration values in selected bacteria" in Botanica Serbica, 40, no. 1 (2016):29-36,
https://doi.org/10.5281/zenodo.48751 . .

TY  - JOUR
AU  - Kolarević, Stoimir
AU  - Aborgiba, Mustafa
AU  - Kracun-Kolarević, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Simonović, Predrag
AU  - Simic, Vladica
AU  - Milosković, Aleksandra
AU  - Reischer, Georg
AU  - Farnleitner, Andreas
AU  - Gačić, Zoran
AU  - Milacic, Radmila
AU  - Zuliani, Tea
AU  - Vidmar, Janja
AU  - Pergal, Marija
AU  - Piria, Marina
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1007
AB  - In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Evaluation of Genotoxic Pressure along the Sava River
IS  - 9
VL  - 11
DO  - 10.1371/journal.pone.0162450
ER  - 

@article{
author = "Kolarević, Stoimir and Aborgiba, Mustafa and Kracun-Kolarević, Margareta and Kostić-Vuković, Jovana and Simonović, Predrag and Simic, Vladica and Milosković, Aleksandra and Reischer, Georg and Farnleitner, Andreas and Gačić, Zoran and Milacic, Radmila and Zuliani, Tea and Vidmar, Janja and Pergal, Marija and Piria, Marina and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Evaluation of Genotoxic Pressure along the Sava River",
number = "9",
volume = "11",
doi = "10.1371/journal.pone.0162450"
}

Kolarević, S., Aborgiba, M., Kracun-Kolarević, M., Kostić-Vuković, J., Simonović, P., Simic, V., Milosković, A., Reischer, G., Farnleitner, A., Gačić, Z., Milacic, R., Zuliani, T., Vidmar, J., Pergal, M., Piria, M., Paunović, M.,& Vukovic-Gacic, B.. (2016). Evaluation of Genotoxic Pressure along the Sava River. in PLoS One
Public Library Science, San Francisco., 11(9).
https://doi.org/10.1371/journal.pone.0162450

Kolarević S, Aborgiba M, Kracun-Kolarević M, Kostić-Vuković J, Simonović P, Simic V, Milosković A, Reischer G, Farnleitner A, Gačić Z, Milacic R, Zuliani T, Vidmar J, Pergal M, Piria M, Paunović M, Vukovic-Gacic B. Evaluation of Genotoxic Pressure along the Sava River. in PLoS One. 2016;11(9).
doi:10.1371/journal.pone.0162450 .

Kolarević, Stoimir, Aborgiba, Mustafa, Kracun-Kolarević, Margareta, Kostić-Vuković, Jovana, Simonović, Predrag, Simic, Vladica, Milosković, Aleksandra, Reischer, Georg, Farnleitner, Andreas, Gačić, Zoran, Milacic, Radmila, Zuliani, Tea, Vidmar, Janja, Pergal, Marija, Piria, Marina, Paunović, Momir, Vukovic-Gacic, Branka, "Evaluation of Genotoxic Pressure along the Sava River" in PLoS One, 11, no. 9 (2016),
https://doi.org/10.1371/journal.pone.0162450 . .

TY  - JOUR
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Jovanović, Jovana
AU  - Marković, Vanja
AU  - Illic, Marija
AU  - Simonović, Predrag
AU  - Simic, Vladica
AU  - Gačić, Zoran
AU  - Diamantini, Elena
AU  - Stella, Elisa
AU  - Petrović, Mira
AU  - Majone, Bruno
AU  - Bellin, Alberto
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/977
AB  - In this study a comprehensive genotoxicological survey throughout the upper and middle stretches of Adige river basin is presented. The study was carried out at 7 sites located along the Adige main course and one the most significant tributaries, the Noce creek, both presenting different levels of pollution pressure. To give an insight into the nature of the genotoxic activity we employed the battery of prokaryotic and eukaryotic assays. Mutagenicity in water samples was evaluated by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg - modified comet assay) were studied in blood cells of Salmo cenerinus Nardo, 1847 and Salmo marmoratus Cuvier, 1829. Within the applied bioassays, comet assay showed the highest potential for discriminating the sampling sites which are under lesser extent of pressure (sampling sites 1-Barnes at Bresimo and 4-Noce downstream S. Giustina) from the sites under high pressure (sampling sites 5-Noce at Mezzolombardo and 6/7-Adige upstream and downstream municipality of Trento). Significant correlation between the standard and Fpg - modified comet assay indicated that oxidative stress could be a major contributor to observed DNA damage in collected specimens.
PB  - Elsevier Science Bv, Amsterdam
T2  - Science of the Total Environment
T1  - Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin
EP  - 1391
SP  - 1383
VL  - 571
DO  - 10.1016/j.scitotenv.2016.07.099
ER  - 

@article{
author = "Kracun-Kolarević, Margareta and Kolarević, Stoimir and Jovanović, Jovana and Marković, Vanja and Illic, Marija and Simonović, Predrag and Simic, Vladica and Gačić, Zoran and Diamantini, Elena and Stella, Elisa and Petrović, Mira and Majone, Bruno and Bellin, Alberto and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2016",
abstract = "In this study a comprehensive genotoxicological survey throughout the upper and middle stretches of Adige river basin is presented. The study was carried out at 7 sites located along the Adige main course and one the most significant tributaries, the Noce creek, both presenting different levels of pollution pressure. To give an insight into the nature of the genotoxic activity we employed the battery of prokaryotic and eukaryotic assays. Mutagenicity in water samples was evaluated by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg - modified comet assay) were studied in blood cells of Salmo cenerinus Nardo, 1847 and Salmo marmoratus Cuvier, 1829. Within the applied bioassays, comet assay showed the highest potential for discriminating the sampling sites which are under lesser extent of pressure (sampling sites 1-Barnes at Bresimo and 4-Noce downstream S. Giustina) from the sites under high pressure (sampling sites 5-Noce at Mezzolombardo and 6/7-Adige upstream and downstream municipality of Trento). Significant correlation between the standard and Fpg - modified comet assay indicated that oxidative stress could be a major contributor to observed DNA damage in collected specimens.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Science of the Total Environment",
title = "Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin",
pages = "1391-1383",
volume = "571",
doi = "10.1016/j.scitotenv.2016.07.099"
}

Kracun-Kolarević, M., Kolarević, S., Jovanović, J., Marković, V., Illic, M., Simonović, P., Simic, V., Gačić, Z., Diamantini, E., Stella, E., Petrović, M., Majone, B., Bellin, A., Paunović, M.,& Vukovic-Gacic, B.. (2016). Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin. in Science of the Total Environment
Elsevier Science Bv, Amsterdam., 571, 1383-1391.
https://doi.org/10.1016/j.scitotenv.2016.07.099

Kracun-Kolarević M, Kolarević S, Jovanović J, Marković V, Illic M, Simonović P, Simic V, Gačić Z, Diamantini E, Stella E, Petrović M, Majone B, Bellin A, Paunović M, Vukovic-Gacic B. Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin. in Science of the Total Environment. 2016;571:1383-1391.
doi:10.1016/j.scitotenv.2016.07.099 .

Kracun-Kolarević, Margareta, Kolarević, Stoimir, Jovanović, Jovana, Marković, Vanja, Illic, Marija, Simonović, Predrag, Simic, Vladica, Gačić, Zoran, Diamantini, Elena, Stella, Elisa, Petrović, Mira, Majone, Bruno, Bellin, Alberto, Paunović, Momir, Vukovic-Gacic, Branka, "Evaluation of genotoxic potential throughout the upper and middle stretches of Adige river basin" in Science of the Total Environment, 571 (2016):1383-1391,
https://doi.org/10.1016/j.scitotenv.2016.07.099 . .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Aborgiba, Mustafa
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1023
AB  - This study examines the use of freshwater bream (Abramis brama) as a sentinel organism for genotoxicity assessment of the Danube River using the comet assay. Sampling of bream was performed during February, April, August, and November in 2014 to assess seasonal variation of DNA damage level as a response to genotoxicity in annual cycle. Additionally, concentrations of fecal coliforms and enterococci were analyzed and they indicated a critical to strong level of fecal pollution on investigated locality during annual cycle. Comet assay was performed on blood, liver, and gill cells of bream. DNA damage level was expressed using tail intensity (TI %), Olive tail moment (OTM), and tail length (TL pix). According to TI and OTM, all three tissues had the highest level of DNA damage in August. The lowest level of DNA damage in liver was measured during February, in blood during November, and in gills during April. According to TL, gills had the highest level of DNA damage in February, and liver cells had the lowest level of damage during April. Multiple correspondence analysis (MCA) showed that DNA damage in blood cells is under the strong influence of variations in NO2, NO3 (-), NH4 (+) levels and also the variation in temperature and oxygen levels. DNA damage in liver cells is highly associated with the variations of Mn, Fe, Cu, Zn, and PO4 (3-) levels. DNA damage in gill cells is strongly affected by the variations of As, Cd, Pb, Cr, and COD (Mn) levels. Freshwater bream is shown to be a potentially good indicator organism in genotoxic potential field studies.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama)
EP  - 20795
IS  - 20
SP  - 20783
VL  - 23
DO  - 10.1007/s11356-016-7213-0
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Aborgiba, Mustafa and Gačić, Zoran and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2016",
abstract = "This study examines the use of freshwater bream (Abramis brama) as a sentinel organism for genotoxicity assessment of the Danube River using the comet assay. Sampling of bream was performed during February, April, August, and November in 2014 to assess seasonal variation of DNA damage level as a response to genotoxicity in annual cycle. Additionally, concentrations of fecal coliforms and enterococci were analyzed and they indicated a critical to strong level of fecal pollution on investigated locality during annual cycle. Comet assay was performed on blood, liver, and gill cells of bream. DNA damage level was expressed using tail intensity (TI %), Olive tail moment (OTM), and tail length (TL pix). According to TI and OTM, all three tissues had the highest level of DNA damage in August. The lowest level of DNA damage in liver was measured during February, in blood during November, and in gills during April. According to TL, gills had the highest level of DNA damage in February, and liver cells had the lowest level of damage during April. Multiple correspondence analysis (MCA) showed that DNA damage in blood cells is under the strong influence of variations in NO2, NO3 (-), NH4 (+) levels and also the variation in temperature and oxygen levels. DNA damage in liver cells is highly associated with the variations of Mn, Fe, Cu, Zn, and PO4 (3-) levels. DNA damage in gill cells is strongly affected by the variations of As, Cd, Pb, Cr, and COD (Mn) levels. Freshwater bream is shown to be a potentially good indicator organism in genotoxic potential field studies.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama)",
pages = "20795-20783",
number = "20",
volume = "23",
doi = "10.1007/s11356-016-7213-0"
}

Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Aborgiba, M., Gačić, Z., Lenhardt, M.,& Vukovic-Gacic, B.. (2016). Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama). in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 23(20), 20783-20795.
https://doi.org/10.1007/s11356-016-7213-0

Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Aborgiba M, Gačić Z, Lenhardt M, Vukovic-Gacic B. Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama). in Environmental Science and Pollution Research. 2016;23(20):20783-20795.
doi:10.1007/s11356-016-7213-0 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Aborgiba, Mustafa, Gačić, Zoran, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Genotoxicity assessment of the Danube River using tissues of freshwater bream (Abramis brama)" in Environmental Science and Pollution Research, 23, no. 20 (2016):20783-20795,
https://doi.org/10.1007/s11356-016-7213-0 . .