TY  - JOUR
AU  - Jovanović-Marić, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Deutschmann, Bjorn
AU  - Hollert, Henner
AU  - Tenji, Dina
AU  - Paunović, Momir
AU  - Vukovic-Gacic, Branka
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1362
AB  - In this study, few different evaluation concepts were used for the assessment of genotoxic potential at the stretch of the Danube River identified as a significant hotspot of pollution originated through the untreated wastewaters. Three sites were chosen: one site upstream of the wastewater outlet in Novi Sad (Serbia), one at the outlet of wastewaters, and one site few kilometer downstream. Ex situ approach comprised prokaryotic SOS/umuCtest onSalmonella typhimuriumTA1535/pSK1005 and comet assay on human hepatoma cell line (HepG2). In situ approach was based on the active monitoring (cage approach) using freshwater musselsSinanodonta woodianaand fishCyprinus carpio.The comet and micronucleus assays were selected for evaluation of DNA damage in mussel haemocytes and fish blood cells. Within the ex situ part of the study, our results indicated that the eukaryotic model system is more sensitive compared to the prokaryotic one. In situ bioassays are recommended for obtaining a better insight into ecosystem status and in the case of our study the complete insight of genotoxic pressure. However, the choice of animals as bioindicators also has a significant impact on the quality of the obtained information. Differential response between fish and mussels was observed at the highly polluted site suggesting possible involvement of additional protective mechanism such as valve closure in mussels.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments
EP  - 33915
IS  - 27
SP  - 33903
VL  - 27
DO  - 10.1007/s11356-020-09597-0
ER  - 

@article{
author = "Jovanović-Marić, Jovana and Kracun-Kolarević, Margareta and Kolarević, Stoimir and Sunjog, Karolina and Kostić-Vuković, Jovana and Deutschmann, Bjorn and Hollert, Henner and Tenji, Dina and Paunović, Momir and Vukovic-Gacic, Branka",
year = "2020",
abstract = "In this study, few different evaluation concepts were used for the assessment of genotoxic potential at the stretch of the Danube River identified as a significant hotspot of pollution originated through the untreated wastewaters. Three sites were chosen: one site upstream of the wastewater outlet in Novi Sad (Serbia), one at the outlet of wastewaters, and one site few kilometer downstream. Ex situ approach comprised prokaryotic SOS/umuCtest onSalmonella typhimuriumTA1535/pSK1005 and comet assay on human hepatoma cell line (HepG2). In situ approach was based on the active monitoring (cage approach) using freshwater musselsSinanodonta woodianaand fishCyprinus carpio.The comet and micronucleus assays were selected for evaluation of DNA damage in mussel haemocytes and fish blood cells. Within the ex situ part of the study, our results indicated that the eukaryotic model system is more sensitive compared to the prokaryotic one. In situ bioassays are recommended for obtaining a better insight into ecosystem status and in the case of our study the complete insight of genotoxic pressure. However, the choice of animals as bioindicators also has a significant impact on the quality of the obtained information. Differential response between fish and mussels was observed at the highly polluted site suggesting possible involvement of additional protective mechanism such as valve closure in mussels.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments",
pages = "33915-33903",
number = "27",
volume = "27",
doi = "10.1007/s11356-020-09597-0"
}

Jovanović-Marić, J., Kracun-Kolarević, M., Kolarević, S., Sunjog, K., Kostić-Vuković, J., Deutschmann, B., Hollert, H., Tenji, D., Paunović, M.,& Vukovic-Gacic, B.. (2020). Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 27(27), 33903-33915.
https://doi.org/10.1007/s11356-020-09597-0

Jovanović-Marić J, Kracun-Kolarević M, Kolarević S, Sunjog K, Kostić-Vuković J, Deutschmann B, Hollert H, Tenji D, Paunović M, Vukovic-Gacic B. Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments. in Environmental Science and Pollution Research. 2020;27(27):33903-33915.
doi:10.1007/s11356-020-09597-0 .

Jovanović-Marić, Jovana, Kracun-Kolarević, Margareta, Kolarević, Stoimir, Sunjog, Karolina, Kostić-Vuković, Jovana, Deutschmann, Bjorn, Hollert, Henner, Tenji, Dina, Paunović, Momir, Vukovic-Gacic, Branka, "Selection of assay, organism, and approach in biomonitoring significantly affects the evaluation of genotoxic potential in aquatic environments" in Environmental Science and Pollution Research, 27, no. 27 (2020):33903-33915,
https://doi.org/10.1007/s11356-020-09597-0 . .

TY  - CONF
AU  - Jovanović Marić, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Kostić-Vuković, Jovana
AU  - Tenji, Dina
AU  - Teodorović, Ivana
AU  - Sipos, Sandor
AU  - Paunović, Momir
AU  - Deutschmann, Bjorn
AU  - Seiler, Thomas-Benjamin
AU  - Vuković-Gačić, Branka
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1728
AB  - Ispuštanje otpadnih voda u površinske vode predstavlja globalni problem jer negativno utiče na kvalitet voda, vodene organizme i zdravlje ljudi. U Srbiji se prerađuje svega 5–10% otpadnih voda.1 U ovoj studiji praćen je uticaj otpadnih voda na nivo DNK oštećenja školjki (Sinanodonta woodiana L.) i riba (Cyprinus carpio L.). Oštećenja DNK u ćelijama hemolimfe i krvi praćeno je alkalnim komet testom (Single Cell Gel Electroforesis, SCGE), koji detektuje biomarkere izlaganja i mikronukleus testom, kojim se prate biomarkeri efekta. Kavezi sa organizmima koji su aklimatizovani u laboratorijskim uslovima su stavljeni na tri lokaliteta na Dunavu: uzvodno od Novog Sada, neposredno nizvodno od ispusta gradske kanalizacije i 7 km nizvodno od Novog Sada. Sa svakog lokaliteta je analizirano 5 jedinki školjki i 10 jedinki riba. a kontrolu su izabrane aklimatizovane jedinke koje nisu izlagane na lokalitetima. Rezultati komet testa pokazali su da je najviši nivo DNK oštećenja u odnosu na kontrolu uočen kod jedinki na mestu ispusta kanalizacije, što je u skladu sa dobijenim hemijskim analizama na ispitivanim lokalitetima.2,3 Za razliku od komet testa, rezultati mikronukleus testa nisu pokazali statistički značajnu razliku između kontrole i odabranih lokaliteta. Dobijeni rezultati ukazuju da su odabrani testovi pokazali različitu oseljivost, jer detektuju različite tipove biomarkera. Međutim, oba testa su ukazala na najviši nivo DNK oštećenja na lokalitetu koji je najizloženiji polutantima.
PB  - Srpsko biološko društvo
C3  - Drugi kongres biologa Srbije, Kladovo, Republika Srbija, 2018
T1  - Praćenje otpadnih voda na Sinanodonta woodiana i Cyprinus carpio u aktivnom biomonitoringu – ekogenotoksikološka studija
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1728
ER  - 

@conference{
author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Kostić-Vuković, Jovana and Tenji, Dina and Teodorović, Ivana and Sipos, Sandor and Paunović, Momir and Deutschmann, Bjorn and Seiler, Thomas-Benjamin and Vuković-Gačić, Branka",
year = "2018",
abstract = "Ispuštanje otpadnih voda u površinske vode predstavlja globalni problem jer negativno utiče na kvalitet voda, vodene organizme i zdravlje ljudi. U Srbiji se prerađuje svega 5–10% otpadnih voda.1 U ovoj studiji praćen je uticaj otpadnih voda na nivo DNK oštećenja školjki (Sinanodonta woodiana L.) i riba (Cyprinus carpio L.). Oštećenja DNK u ćelijama hemolimfe i krvi praćeno je alkalnim komet testom (Single Cell Gel Electroforesis, SCGE), koji detektuje biomarkere izlaganja i mikronukleus testom, kojim se prate biomarkeri efekta. Kavezi sa organizmima koji su aklimatizovani u laboratorijskim uslovima su stavljeni na tri lokaliteta na Dunavu: uzvodno od Novog Sada, neposredno nizvodno od ispusta gradske kanalizacije i 7 km nizvodno od Novog Sada. Sa svakog lokaliteta je analizirano 5 jedinki školjki i 10 jedinki riba. a kontrolu su izabrane aklimatizovane jedinke koje nisu izlagane na lokalitetima. Rezultati komet testa pokazali su da je najviši nivo DNK oštećenja u odnosu na kontrolu uočen kod jedinki na mestu ispusta kanalizacije, što je u skladu sa dobijenim hemijskim analizama na ispitivanim lokalitetima.2,3 Za razliku od komet testa, rezultati mikronukleus testa nisu pokazali statistički značajnu razliku između kontrole i odabranih lokaliteta. Dobijeni rezultati ukazuju da su odabrani testovi pokazali različitu oseljivost, jer detektuju različite tipove biomarkera. Međutim, oba testa su ukazala na najviši nivo DNK oštećenja na lokalitetu koji je najizloženiji polutantima.",
publisher = "Srpsko biološko društvo",
journal = "Drugi kongres biologa Srbije, Kladovo, Republika Srbija, 2018",
title = "Praćenje otpadnih voda na Sinanodonta woodiana i Cyprinus carpio u aktivnom biomonitoringu – ekogenotoksikološka studija",
pages = "110",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1728"
}

Jovanović Marić, J., Kolarević, S., Kračun-Kolarević, M., Sunjog, K., Kostić-Vuković, J., Tenji, D., Teodorović, I., Sipos, S., Paunović, M., Deutschmann, B., Seiler, T.,& Vuković-Gačić, B.. (2018). Praćenje otpadnih voda na Sinanodonta woodiana i Cyprinus carpio u aktivnom biomonitoringu – ekogenotoksikološka studija. in Drugi kongres biologa Srbije, Kladovo, Republika Srbija, 2018
Srpsko biološko društvo., 110.
https://hdl.handle.net/21.15107/rcub_rimsi_1728

Jovanović Marić J, Kolarević S, Kračun-Kolarević M, Sunjog K, Kostić-Vuković J, Tenji D, Teodorović I, Sipos S, Paunović M, Deutschmann B, Seiler T, Vuković-Gačić B. Praćenje otpadnih voda na Sinanodonta woodiana i Cyprinus carpio u aktivnom biomonitoringu – ekogenotoksikološka studija. in Drugi kongres biologa Srbije, Kladovo, Republika Srbija, 2018. 2018;:110.
https://hdl.handle.net/21.15107/rcub_rimsi_1728 .

Jovanović Marić, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Kostić-Vuković, Jovana, Tenji, Dina, Teodorović, Ivana, Sipos, Sandor, Paunović, Momir, Deutschmann, Bjorn, Seiler, Thomas-Benjamin, Vuković-Gačić, Branka, "Praćenje otpadnih voda na Sinanodonta woodiana i Cyprinus carpio u aktivnom biomonitoringu – ekogenotoksikološka studija" in Drugi kongres biologa Srbije, Kladovo, Republika Srbija, 2018 (2018):110,
https://hdl.handle.net/21.15107/rcub_rimsi_1728 .

TY  - JOUR
AU  - Deutschmann, Bjorn
AU  - Kolarević, Stoimir
AU  - Brack, Werner
AU  - Kaisarević, Sonja
AU  - Kostić-Vuković, Jovana
AU  - Kracun-Kolarević, Margareta
AU  - Liska, Igor
AU  - Paunović, Momir
AU  - Seiler, Thomas-Benjamin
AU  - Shao, Ying
AU  - Sipos, Sandor
AU  - Slobodnik, Jaroslav
AU  - Teodorović, Ivana
AU  - Vukovic-Gacic, Branka
AU  - Hollert, Henner
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/957
AB  - The Joint Danube Survey 3 (JDS3; the biggest river expedition in 2013) had offered the unique opportunity for a large-scale monitoring approach for biomarker response in feral fish collected along a Danube stretch from Kehlheim (DE) to Sulina (RO). The advantage of genotoxicity as a marker for pollution exposure in fish is the early detection of possible long-term effects such as cancer. Therefore, genotoxicity was in the focus of the biomarker investigations in fish during the expedition. Blood samples of common bleak (Alburnus alburnus) for the investigation of the micronucleus frequency and comet tail intensity of fragmented DNA material in erythrocytes were collected at 18 and 12 sampling sites, respectively. For 9 sampling sites same samples were used to compare the in-situ data for the comparable genotoxic endpoint in the micronucleus (MN) and comet assay (CM). The data of both.in-situ assays showed a significant correlation, indicating the strength and comparability of the data sets. Significant variation in DNA damage in-fish along the longitudinal profile of the Danube was demonstrated for both assays compared to reference sites. The results suggest that DNA damage in erythrocytes of fish was mainly affected by wastewater of highly populated regions. No linkage between the results and the general health/dietary status of the fish were revealed, whereas correlation with some genotoxicity drivers in the water phase, suspended particulate matter and sediments could be demonstrated.
PB  - Elsevier, Amsterdam
T2  - Science of the Total Environment
T1  - Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay
EP  - 1449
SP  - 1441
VL  - 573
DO  - 10.1016/j.scitotenv.2016.07.175
ER  - 

@article{
author = "Deutschmann, Bjorn and Kolarević, Stoimir and Brack, Werner and Kaisarević, Sonja and Kostić-Vuković, Jovana and Kracun-Kolarević, Margareta and Liska, Igor and Paunović, Momir and Seiler, Thomas-Benjamin and Shao, Ying and Sipos, Sandor and Slobodnik, Jaroslav and Teodorović, Ivana and Vukovic-Gacic, Branka and Hollert, Henner",
year = "2016",
abstract = "The Joint Danube Survey 3 (JDS3; the biggest river expedition in 2013) had offered the unique opportunity for a large-scale monitoring approach for biomarker response in feral fish collected along a Danube stretch from Kehlheim (DE) to Sulina (RO). The advantage of genotoxicity as a marker for pollution exposure in fish is the early detection of possible long-term effects such as cancer. Therefore, genotoxicity was in the focus of the biomarker investigations in fish during the expedition. Blood samples of common bleak (Alburnus alburnus) for the investigation of the micronucleus frequency and comet tail intensity of fragmented DNA material in erythrocytes were collected at 18 and 12 sampling sites, respectively. For 9 sampling sites same samples were used to compare the in-situ data for the comparable genotoxic endpoint in the micronucleus (MN) and comet assay (CM). The data of both.in-situ assays showed a significant correlation, indicating the strength and comparability of the data sets. Significant variation in DNA damage in-fish along the longitudinal profile of the Danube was demonstrated for both assays compared to reference sites. The results suggest that DNA damage in erythrocytes of fish was mainly affected by wastewater of highly populated regions. No linkage between the results and the general health/dietary status of the fish were revealed, whereas correlation with some genotoxicity drivers in the water phase, suspended particulate matter and sediments could be demonstrated.",
publisher = "Elsevier, Amsterdam",
journal = "Science of the Total Environment",
title = "Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay",
pages = "1449-1441",
volume = "573",
doi = "10.1016/j.scitotenv.2016.07.175"
}

Deutschmann, B., Kolarević, S., Brack, W., Kaisarević, S., Kostić-Vuković, J., Kracun-Kolarević, M., Liska, I., Paunović, M., Seiler, T., Shao, Y., Sipos, S., Slobodnik, J., Teodorović, I., Vukovic-Gacic, B.,& Hollert, H.. (2016). Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay. in Science of the Total Environment
Elsevier, Amsterdam., 573, 1441-1449.
https://doi.org/10.1016/j.scitotenv.2016.07.175

Deutschmann B, Kolarević S, Brack W, Kaisarević S, Kostić-Vuković J, Kracun-Kolarević M, Liska I, Paunović M, Seiler T, Shao Y, Sipos S, Slobodnik J, Teodorović I, Vukovic-Gacic B, Hollert H. Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay. in Science of the Total Environment. 2016;573:1441-1449.
doi:10.1016/j.scitotenv.2016.07.175 .

Deutschmann, Bjorn, Kolarević, Stoimir, Brack, Werner, Kaisarević, Sonja, Kostić-Vuković, Jovana, Kracun-Kolarević, Margareta, Liska, Igor, Paunović, Momir, Seiler, Thomas-Benjamin, Shao, Ying, Sipos, Sandor, Slobodnik, Jaroslav, Teodorović, Ivana, Vukovic-Gacic, Branka, Hollert, Henner, "Longitudinal profile of the genotoxic potential of the River Danube on erythrocytes of wild common bleak (Alburnus alburnus) assessed using the comet and micronucleus assay" in Science of the Total Environment, 573 (2016):1441-1449,
https://doi.org/10.1016/j.scitotenv.2016.07.175 . .