TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Đorđević Aleksić, Jelena
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2173
AB  - One of the most severe threats to the Danube River course through Serbia is the direct discharge of untreated wastewater. In biomonitoring studies, faecal indicator bacteria are used to reveal the presence of untreated communal wastewater, while biomarker response in indigenous biota serves as a useful indicator of their harmful potential. The objective of this study was to estimate the genotoxic potential of untreated communal wastewater at two sites on the Danube River, Višnjica (Belgrade) and Novi Banovci, by using in situ approach in white bream (Blicca bjoerkna L.). A comet assay was selected to measure DNA damage in erythrocytes, liver, and gill cells, while the micronucleus test was applied to measure chromosomal aberrations in erythrocytes.
Additionally, the accumulation of 22 elements in fish liver and muscle was analysed by ICP-OES meth- od. Simultaneous detection of total coliforms and E.coli in water was performed by enzyme-based Colilert-18 test (IDEXX). Comet assay revealed the highest level of DNA damage in gills at the site Novi Banovci, and in blood at the site Višnjica. The overall frequency of micronucleus at both sites was low. At both localities, the majority of analysed elements showed higher levels in the liver in comparison to muscle. Microbiological indicators confirmed the poor water quality, since at both sites water was categorised as critically to strongly polluted. The results of this study highlighted the importance of in situ biomonitoring approach and the need for more effective management of natural resources and implementation of wastewater treatment systems.
PB  - Serbian Society of Toxicology
C3  - 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia
T1  - Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2173
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Sunjog, Karolina and Kolarević, Stoimir and Višnjić Jeftić, Željka and Subotić, Srđan and Đorđević Aleksić, Jelena and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2023",
abstract = "One of the most severe threats to the Danube River course through Serbia is the direct discharge of untreated wastewater. In biomonitoring studies, faecal indicator bacteria are used to reveal the presence of untreated communal wastewater, while biomarker response in indigenous biota serves as a useful indicator of their harmful potential. The objective of this study was to estimate the genotoxic potential of untreated communal wastewater at two sites on the Danube River, Višnjica (Belgrade) and Novi Banovci, by using in situ approach in white bream (Blicca bjoerkna L.). A comet assay was selected to measure DNA damage in erythrocytes, liver, and gill cells, while the micronucleus test was applied to measure chromosomal aberrations in erythrocytes.
Additionally, the accumulation of 22 elements in fish liver and muscle was analysed by ICP-OES meth- od. Simultaneous detection of total coliforms and E.coli in water was performed by enzyme-based Colilert-18 test (IDEXX). Comet assay revealed the highest level of DNA damage in gills at the site Novi Banovci, and in blood at the site Višnjica. The overall frequency of micronucleus at both sites was low. At both localities, the majority of analysed elements showed higher levels in the liver in comparison to muscle. Microbiological indicators confirmed the poor water quality, since at both sites water was categorised as critically to strongly polluted. The results of this study highlighted the importance of in situ biomonitoring approach and the need for more effective management of natural resources and implementation of wastewater treatment systems.",
publisher = "Serbian Society of Toxicology",
journal = "13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia",
title = "Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2173"
}

Kostić-Vuković, J., Sunjog, K., Kolarević, S., Višnjić Jeftić, Ž., Subotić, S., Đorđević Aleksić, J., Lenhardt, M.,& Vuković-Gačić, B.. (2023). Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.. in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia
Serbian Society of Toxicology., 72.
https://hdl.handle.net/21.15107/rcub_rimsi_2173

Kostić-Vuković J, Sunjog K, Kolarević S, Višnjić Jeftić Ž, Subotić S, Đorđević Aleksić J, Lenhardt M, Vuković-Gačić B. Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.. in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia. 2023;:72.
https://hdl.handle.net/21.15107/rcub_rimsi_2173 .

Kostić-Vuković, Jovana, Sunjog, Karolina, Kolarević, Stoimir, Višnjić Jeftić, Željka, Subotić, Srđan, Đorđević Aleksić, Jelena, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment." in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia (2023):72,
https://hdl.handle.net/21.15107/rcub_rimsi_2173 .