TY  - JOUR
AU  - Bogdanović, Svetlana
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Nikolić, Ivan
AU  - Danilović, Bojana
AU  - Stanković, Slaviša
AU  - Dimkić, Ivica
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1247
AB  - The "Pirot 'ironed' sausage" (Pis) is a traditional, dry, fermented product originating from the town of Pirot, situated in southeastern Serbia. This product is made from different types of meat (goat, sheep, beef and donkey) and spices, without additives or starter cultures. Pis is an organic, unprocessed product, without heat or smoke treatment. The aim of this study was to characterize 120 isolates of lactic acid bacteria (LAB) from Pis produced by six different brands during a two-year period using phenotypic and genetic identification. Preliminary characterization of the LAB was based on general morphology and biochemical tests. Repetitive elements such as REP, BOX and GTG(5), found in the genome of these bacteria, and randomly amplified polymorphic DNA-polymerase chain reaction (RAPD PCR) sequences were used for determination of genetic polymorphism. Identification by 16S rRNA gene sequencing showed the presence of only two LAB species, Lactobacillus sakei (76%) and Leuconostoc mesenteroides (24%). However, genetic polymorphism was detected using fingerprinting methods. In comparison with other primers, the profiles obtained with GTG(5) showed the highest heterogeneity for most of the tested isolates, with sequencing results additionally confirming its discriminatory power. In addition, M13 RAPD primer also produced satisfactory separation of the tested isolates.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Genetic polymorphism of lactic acid bacteria isolated from "Pirot 'ironed' sausage" from Serbia
EP  - 102
IS  - 1
SP  - 95
VL  - 71
DO  - 10.2298/ABS180920051B
ER  - 

@article{
author = "Bogdanović, Svetlana and Jelušić, Aleksandra and Berić, Tanja and Nikolić, Ivan and Danilović, Bojana and Stanković, Slaviša and Dimkić, Ivica",
year = "2019",
abstract = "The "Pirot 'ironed' sausage" (Pis) is a traditional, dry, fermented product originating from the town of Pirot, situated in southeastern Serbia. This product is made from different types of meat (goat, sheep, beef and donkey) and spices, without additives or starter cultures. Pis is an organic, unprocessed product, without heat or smoke treatment. The aim of this study was to characterize 120 isolates of lactic acid bacteria (LAB) from Pis produced by six different brands during a two-year period using phenotypic and genetic identification. Preliminary characterization of the LAB was based on general morphology and biochemical tests. Repetitive elements such as REP, BOX and GTG(5), found in the genome of these bacteria, and randomly amplified polymorphic DNA-polymerase chain reaction (RAPD PCR) sequences were used for determination of genetic polymorphism. Identification by 16S rRNA gene sequencing showed the presence of only two LAB species, Lactobacillus sakei (76%) and Leuconostoc mesenteroides (24%). However, genetic polymorphism was detected using fingerprinting methods. In comparison with other primers, the profiles obtained with GTG(5) showed the highest heterogeneity for most of the tested isolates, with sequencing results additionally confirming its discriminatory power. In addition, M13 RAPD primer also produced satisfactory separation of the tested isolates.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Genetic polymorphism of lactic acid bacteria isolated from "Pirot 'ironed' sausage" from Serbia",
pages = "102-95",
number = "1",
volume = "71",
doi = "10.2298/ABS180920051B"
}

Bogdanović, S., Jelušić, A., Berić, T., Nikolić, I., Danilović, B., Stanković, S.,& Dimkić, I.. (2019). Genetic polymorphism of lactic acid bacteria isolated from "Pirot 'ironed' sausage" from Serbia. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 71(1), 95-102.
https://doi.org/10.2298/ABS180920051B

Bogdanović S, Jelušić A, Berić T, Nikolić I, Danilović B, Stanković S, Dimkić I. Genetic polymorphism of lactic acid bacteria isolated from "Pirot 'ironed' sausage" from Serbia. in Archives of Biological Sciences. 2019;71(1):95-102.
doi:10.2298/ABS180920051B .

Bogdanović, Svetlana, Jelušić, Aleksandra, Berić, Tanja, Nikolić, Ivan, Danilović, Bojana, Stanković, Slaviša, Dimkić, Ivica, "Genetic polymorphism of lactic acid bacteria isolated from "Pirot 'ironed' sausage" from Serbia" in Archives of Biological Sciences, 71, no. 1 (2019):95-102,
https://doi.org/10.2298/ABS180920051B . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Marjanović-Jeromela, Ana
AU  - Nikolić, Ivan
AU  - Stanković, Slaviša
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1215
AB  - The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia
EP  - 1457
IS  - 8
SP  - 1448
VL  - 68
DO  - 10.1111/ppa.13064
ER  - 

@article{
author = "Popović, Tatjana and Mitrović, Petar and Jelušić, Aleksandra and Dimkić, Ivica and Marjanović-Jeromela, Ana and Nikolić, Ivan and Stanković, Slaviša",
year = "2019",
abstract = "The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia",
pages = "1457-1448",
number = "8",
volume = "68",
doi = "10.1111/ppa.13064"
}

Popović, T., Mitrović, P., Jelušić, A., Dimkić, I., Marjanović-Jeromela, A., Nikolić, I.,& Stanković, S.. (2019). Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology
Wiley, Hoboken., 68(8), 1448-1457.
https://doi.org/10.1111/ppa.13064

Popović T, Mitrović P, Jelušić A, Dimkić I, Marjanović-Jeromela A, Nikolić I, Stanković S. Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology. 2019;68(8):1448-1457.
doi:10.1111/ppa.13064 .

Popović, Tatjana, Mitrović, Petar, Jelušić, Aleksandra, Dimkić, Ivica, Marjanović-Jeromela, Ana, Nikolić, Ivan, Stanković, Slaviša, "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia" in Plant Pathology, 68, no. 8 (2019):1448-1457,
https://doi.org/10.1111/ppa.13064 . .

TY  - JOUR
AU  - Pejin, Boris
AU  - Stosic-Grujicic, Stanislava
AU  - Bogdanović, Gordana
AU  - Hegediš, Aleksandar
AU  - Karaman, I.
AU  - Stojanović, I.
AU  - Nikolić, Ivan
AU  - Kojic, V.
AU  - Horvatović, Mladen
AU  - Radotić, Ksenija
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/619
AB  - The immunomodulatory and anticarcinogenic activity of the freshwater bryozoan Hyalinella punctata methanolic extract (MEx) was evaluated in vitro on selected biosystems for the first time. Murine lymphocytes and macrophages were used for testing of MEx effects on cell proliferation and nitric oxide (NO) production, respectively, while human cancer cell lines were utilized for measuring its activity against cancer cells. The results suggest a strong and tissue-specific immunomodifying activity (IC50 values for inhibition of proliferation of lymph node and spleen-derived lymphocytes were 4.1 mu g/ml and 9.7 mu g/ml, respectively) and moderate anticancer activity of MEx (IC50 value for the MCF-7 cell line was 24.13 mu g/ml). Down-regulation of macrophage NO production was also obtained. The potential use of H. punctata-derived natural products for the treatment of human chronic inflammatory diseases and cancer is worthy of further investigation.
PB  - Inst Materials Physics
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - In vitro evaluation of the immunomodulatory and anticarcinogenic activity of the freshwater bryozoan hyalinella punctata methanolic extract
EP  - 195
IS  - 1
SP  - 187
VL  - 8
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_619
ER  - 

@article{
author = "Pejin, Boris and Stosic-Grujicic, Stanislava and Bogdanović, Gordana and Hegediš, Aleksandar and Karaman, I. and Stojanović, I. and Nikolić, Ivan and Kojic, V. and Horvatović, Mladen and Radotić, Ksenija",
year = "2012",
abstract = "The immunomodulatory and anticarcinogenic activity of the freshwater bryozoan Hyalinella punctata methanolic extract (MEx) was evaluated in vitro on selected biosystems for the first time. Murine lymphocytes and macrophages were used for testing of MEx effects on cell proliferation and nitric oxide (NO) production, respectively, while human cancer cell lines were utilized for measuring its activity against cancer cells. The results suggest a strong and tissue-specific immunomodifying activity (IC50 values for inhibition of proliferation of lymph node and spleen-derived lymphocytes were 4.1 mu g/ml and 9.7 mu g/ml, respectively) and moderate anticancer activity of MEx (IC50 value for the MCF-7 cell line was 24.13 mu g/ml). Down-regulation of macrophage NO production was also obtained. The potential use of H. punctata-derived natural products for the treatment of human chronic inflammatory diseases and cancer is worthy of further investigation.",
publisher = "Inst Materials Physics",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "In vitro evaluation of the immunomodulatory and anticarcinogenic activity of the freshwater bryozoan hyalinella punctata methanolic extract",
pages = "195-187",
number = "1",
volume = "8",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_619"
}

Pejin, B., Stosic-Grujicic, S., Bogdanović, G., Hegediš, A., Karaman, I., Stojanović, I., Nikolić, I., Kojic, V., Horvatović, M.,& Radotić, K.. (2012). In vitro evaluation of the immunomodulatory and anticarcinogenic activity of the freshwater bryozoan hyalinella punctata methanolic extract. in Digest Journal of Nanomaterials and Biostructures
Inst Materials Physics., 8(1), 187-195.
https://hdl.handle.net/21.15107/rcub_rimsi_619

Pejin B, Stosic-Grujicic S, Bogdanović G, Hegediš A, Karaman I, Stojanović I, Nikolić I, Kojic V, Horvatović M, Radotić K. In vitro evaluation of the immunomodulatory and anticarcinogenic activity of the freshwater bryozoan hyalinella punctata methanolic extract. in Digest Journal of Nanomaterials and Biostructures. 2012;8(1):187-195.
https://hdl.handle.net/21.15107/rcub_rimsi_619 .

Pejin, Boris, Stosic-Grujicic, Stanislava, Bogdanović, Gordana, Hegediš, Aleksandar, Karaman, I., Stojanović, I., Nikolić, Ivan, Kojic, V., Horvatović, Mladen, Radotić, Ksenija, "In vitro evaluation of the immunomodulatory and anticarcinogenic activity of the freshwater bryozoan hyalinella punctata methanolic extract" in Digest Journal of Nanomaterials and Biostructures, 8, no. 1 (2012):187-195,
https://hdl.handle.net/21.15107/rcub_rimsi_619 .