TY  - JOUR
AU  - Bijelic, Dunja D.
AU  - Milicević, Katarina
AU  - Lazarević, Milica N.
AU  - Miljković, Đorđe
AU  - Bogdanović Pristov, Jelena
AU  - Savić, Danijela Z
AU  - Petković, Branka
AU  - Andjus, Pavle R.
AU  - Momcilović, Miljana
AU  - Nikolić, Ljiljana M.
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1317
AB  - Interaction between autoreactive immune cells and astroglia is an important part of the pathologic processes that fuel neurodegeneration in multiple sclerosis. In this inflammatory disease, immune cells enter into the central nervous system (CNS) and they spread through CNS parenchyma, but the impact of these autoreactive immune cells on the activity pattern of astrocytes has not been defined. By exploiting naive astrocytes in culture and CNS-infiltrated immune cells (CNS IICs) isolated from rat with experimental autoimmune encephalomyelitis (EAE), here we demonstrate previously unrecognized properties of immune cell-astrocyte interaction. We show that CNS IICs but not the peripheral immune cell application, evokes a rapid and vigorous intracellular Ca(2+)increase in astrocytes by promoting glial release of ATP. ATP propagated Ca(2+)elevation through glial purinergic P2X7 receptor activation by the hemichannel-dependent nucleotide release mechanism. Astrocyte Ca(2+)increase is specifically triggered by the autoreactive CD4(+)T-cell application and these two cell types exhibit close spatial interaction in EAE. Therefore, Ca(2+)signals may mediate a rapid astroglial response to the autoreactive immune cells in their local environment. This property of immune cell-astrocyte interaction may be important to consider in studies interrogating CNS autoimmune disease.
PB  - Wiley, Hoboken
T2  - Journal of Neuroscience Research
T1  - Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling
EP  - 2332
IS  - 11
SP  - 2317
VL  - 98
DO  - 10.1002/jnr.24699
ER  - 

@article{
author = "Bijelic, Dunja D. and Milicević, Katarina and Lazarević, Milica N. and Miljković, Đorđe and Bogdanović Pristov, Jelena and Savić, Danijela Z and Petković, Branka and Andjus, Pavle R. and Momcilović, Miljana and Nikolić, Ljiljana M.",
year = "2020",
abstract = "Interaction between autoreactive immune cells and astroglia is an important part of the pathologic processes that fuel neurodegeneration in multiple sclerosis. In this inflammatory disease, immune cells enter into the central nervous system (CNS) and they spread through CNS parenchyma, but the impact of these autoreactive immune cells on the activity pattern of astrocytes has not been defined. By exploiting naive astrocytes in culture and CNS-infiltrated immune cells (CNS IICs) isolated from rat with experimental autoimmune encephalomyelitis (EAE), here we demonstrate previously unrecognized properties of immune cell-astrocyte interaction. We show that CNS IICs but not the peripheral immune cell application, evokes a rapid and vigorous intracellular Ca(2+)increase in astrocytes by promoting glial release of ATP. ATP propagated Ca(2+)elevation through glial purinergic P2X7 receptor activation by the hemichannel-dependent nucleotide release mechanism. Astrocyte Ca(2+)increase is specifically triggered by the autoreactive CD4(+)T-cell application and these two cell types exhibit close spatial interaction in EAE. Therefore, Ca(2+)signals may mediate a rapid astroglial response to the autoreactive immune cells in their local environment. This property of immune cell-astrocyte interaction may be important to consider in studies interrogating CNS autoimmune disease.",
publisher = "Wiley, Hoboken",
journal = "Journal of Neuroscience Research",
title = "Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling",
pages = "2332-2317",
number = "11",
volume = "98",
doi = "10.1002/jnr.24699"
}

Bijelic, D. D., Milicević, K., Lazarević, M. N., Miljković, Đ., Bogdanović Pristov, J., Savić, D. Z., Petković, B., Andjus, P. R., Momcilović, M.,& Nikolić, L. M.. (2020). Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling. in Journal of Neuroscience Research
Wiley, Hoboken., 98(11), 2317-2332.
https://doi.org/10.1002/jnr.24699

Bijelic DD, Milicević K, Lazarević MN, Miljković Đ, Bogdanović Pristov J, Savić DZ, Petković B, Andjus PR, Momcilović M, Nikolić LM. Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling. in Journal of Neuroscience Research. 2020;98(11):2317-2332.
doi:10.1002/jnr.24699 .

Bijelic, Dunja D., Milicević, Katarina, Lazarević, Milica N., Miljković, Đorđe, Bogdanović Pristov, Jelena, Savić, Danijela Z, Petković, Branka, Andjus, Pavle R., Momcilović, Miljana, Nikolić, Ljiljana M., "Central nervous system-infiltrated immune cells induce calcium increase in astrocytes via astroglial purinergic signaling" in Journal of Neuroscience Research, 98, no. 11 (2020):2317-2332,
https://doi.org/10.1002/jnr.24699 . .

TY  - JOUR
AU  - Miljković, Đorđe
AU  - Blazevski, Jana
AU  - Petković, Filip
AU  - Djedović, Neda
AU  - Momcilović, Miljana
AU  - Stanisavljević, Suzana
AU  - Jevtic, Bojan
AU  - Mostarica-Stojković, Marija
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/891
AB  - Dimethyl fumarate (DMF), a new drug for multiple sclerosis (MS) treatment, acts against neuroinflammation via mechanisms that are triggered by adduct formation with thiol redox switches. Ethyl pyruvate (EP), an off-the-shelf agent, appears to be a redox analog of DMF, but its immunomodulatory properties have not been put into the context of MS therapy. In this article, we examined and compared the effects of EP and DMF on MS-relevant activity/functions of T cells, macrophages, microglia, and astrocytes. EP efficiently suppressed the release of MS signature cytokines, IFN-gamma and IL-17, from human PBMCs. Furthermore, the production of these cytokines was notably decreased in encephalitogenic T cells after in vivo application of EP to rats. Production of two other proinflammatory cytokines, IL-6 and TNF, and NO was suppressed by EP in macrophages and microglia. Reactive oxygen species production in macrophages, microglia activation, and the development of Ag-presenting phenotype in microglia and macrophages were constrained by EP. The release of IL-6 was reduced in astrocytes. Finally, EP inhibited the activation of transcription factor NF-kappa B in microglia and astrocytes. Most of these effects were also found for DMF, implying that EP and DMF share common targets and mechanisms of action. Importantly, EP had in vivo impact on experimental autoimmune encephalomyelitis, an animal model of MS. Treatment with EP resulted in delay and shortening of the first relapse, and lower clinical scores, whereas the second attack was annihilated. Further studies on the possibility to use EP as an MS therapeutic are warranted.
PB  - Amer Assoc Immunologists, Bethesda
T2  - Journal of Immunology
T1  - A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory Properties of Ethyl Pyruvate and Dimethyl Fumarate
EP  - 2503
IS  - 6
SP  - 2493
VL  - 194
DO  - 10.4049/jimmunol.1402302
ER  - 

@article{
author = "Miljković, Đorđe and Blazevski, Jana and Petković, Filip and Djedović, Neda and Momcilović, Miljana and Stanisavljević, Suzana and Jevtic, Bojan and Mostarica-Stojković, Marija and Spasojević, Ivan",
year = "2015",
abstract = "Dimethyl fumarate (DMF), a new drug for multiple sclerosis (MS) treatment, acts against neuroinflammation via mechanisms that are triggered by adduct formation with thiol redox switches. Ethyl pyruvate (EP), an off-the-shelf agent, appears to be a redox analog of DMF, but its immunomodulatory properties have not been put into the context of MS therapy. In this article, we examined and compared the effects of EP and DMF on MS-relevant activity/functions of T cells, macrophages, microglia, and astrocytes. EP efficiently suppressed the release of MS signature cytokines, IFN-gamma and IL-17, from human PBMCs. Furthermore, the production of these cytokines was notably decreased in encephalitogenic T cells after in vivo application of EP to rats. Production of two other proinflammatory cytokines, IL-6 and TNF, and NO was suppressed by EP in macrophages and microglia. Reactive oxygen species production in macrophages, microglia activation, and the development of Ag-presenting phenotype in microglia and macrophages were constrained by EP. The release of IL-6 was reduced in astrocytes. Finally, EP inhibited the activation of transcription factor NF-kappa B in microglia and astrocytes. Most of these effects were also found for DMF, implying that EP and DMF share common targets and mechanisms of action. Importantly, EP had in vivo impact on experimental autoimmune encephalomyelitis, an animal model of MS. Treatment with EP resulted in delay and shortening of the first relapse, and lower clinical scores, whereas the second attack was annihilated. Further studies on the possibility to use EP as an MS therapeutic are warranted.",
publisher = "Amer Assoc Immunologists, Bethesda",
journal = "Journal of Immunology",
title = "A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory Properties of Ethyl Pyruvate and Dimethyl Fumarate",
pages = "2503-2493",
number = "6",
volume = "194",
doi = "10.4049/jimmunol.1402302"
}

Miljković, Đ., Blazevski, J., Petković, F., Djedović, N., Momcilović, M., Stanisavljević, S., Jevtic, B., Mostarica-Stojković, M.,& Spasojević, I.. (2015). A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory Properties of Ethyl Pyruvate and Dimethyl Fumarate. in Journal of Immunology
Amer Assoc Immunologists, Bethesda., 194(6), 2493-2503.
https://doi.org/10.4049/jimmunol.1402302

Miljković Đ, Blazevski J, Petković F, Djedović N, Momcilović M, Stanisavljević S, Jevtic B, Mostarica-Stojković M, Spasojević I. A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory Properties of Ethyl Pyruvate and Dimethyl Fumarate. in Journal of Immunology. 2015;194(6):2493-2503.
doi:10.4049/jimmunol.1402302 .

Miljković, Đorđe, Blazevski, Jana, Petković, Filip, Djedović, Neda, Momcilović, Miljana, Stanisavljević, Suzana, Jevtic, Bojan, Mostarica-Stojković, Marija, Spasojević, Ivan, "A Comparative Analysis of Multiple Sclerosis-Relevant Anti-Inflammatory Properties of Ethyl Pyruvate and Dimethyl Fumarate" in Journal of Immunology, 194, no. 6 (2015):2493-2503,
https://doi.org/10.4049/jimmunol.1402302 . .

TY  - CONF
AU  - Miljković, Đorđe
AU  - Petković, Filip
AU  - Blazevski, Jana
AU  - Momcilović, Miljana
AU  - Djedović, Neda
AU  - Mostarica-Stojković, Marija
AU  - Spasojević, Ivan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/811
AB  - The ongoing success of dimethyl fumarate (DMF; Tecfidera) opens a perspective for further development of the redox strategy in multiple sclerosis (MS) treatment. The mechanisms of anti-inflammatory and neuroprotective effects of DMF involve the formation of adducts with redox-sensitive thiol switches on the surface of T cell resulting in suppressed activity, proliferation and pro-inflammatory cytokine release, and the activation of antioxidative defense in the central nervous system (CNS) cells. Ethyl pyruvate (EP) appears to be a safer non-toxic redox analogue of DMF, as they share common molecular targets. We examined the effects of EP on CNS resident cells (astrocytes, microglia) and encephalitogenic T cells, as well as its influence on the clinical course of actively induced experimental autoimmune encephalomyelitis (EAE). Astrocytes and microglia were stimulated with pro-inflammatory cytokines and treated with EP in vitro. Encephalitogenic immune cells were isolated from draining lymph nodes of rats that were immunized with myelin basic protein (MBP) and complete Freund's adjuvant (CFA) and treated with EP in vivo. EP modulated cytokine generation in the examined cells in an anti-inflammatory manner: (i) the production of interleukin (IL)-6, but not IL-10, was down-regulated in astrocytes; (ii) the release of IL-6, tumor necrosis factor and nitric oxide from microglial cells was reduced; (iii) the production of interferon-gamma and IL-17, but not IL-10, was suppressed in lymph node cells isolated from MBP + CFA-immunized rats. In addition, EP alleviated EAE course in rats, delaying the onset, shortening the relapse, and lowering clinical scores. These results imply that EP has a potency to inhibit inflammation in the CNS. While further studies on the effect of EP on the CNS inflammation are warranted, we believe that EP might be applicable and beneficial in MS treatment.
PB  - Elsevier Science Bv, Amsterdam
C3  - Journal of Neuroimmunology
T1  - Effect of ethyl pyruvate on central nervous system inflammation
EP  - 224
IS  - 1-2
SP  - 223
VL  - 275
DO  - 10.1016/j.jneuroim.2014.08.600
ER  - 

@conference{
author = "Miljković, Đorđe and Petković, Filip and Blazevski, Jana and Momcilović, Miljana and Djedović, Neda and Mostarica-Stojković, Marija and Spasojević, Ivan",
year = "2014",
abstract = "The ongoing success of dimethyl fumarate (DMF; Tecfidera) opens a perspective for further development of the redox strategy in multiple sclerosis (MS) treatment. The mechanisms of anti-inflammatory and neuroprotective effects of DMF involve the formation of adducts with redox-sensitive thiol switches on the surface of T cell resulting in suppressed activity, proliferation and pro-inflammatory cytokine release, and the activation of antioxidative defense in the central nervous system (CNS) cells. Ethyl pyruvate (EP) appears to be a safer non-toxic redox analogue of DMF, as they share common molecular targets. We examined the effects of EP on CNS resident cells (astrocytes, microglia) and encephalitogenic T cells, as well as its influence on the clinical course of actively induced experimental autoimmune encephalomyelitis (EAE). Astrocytes and microglia were stimulated with pro-inflammatory cytokines and treated with EP in vitro. Encephalitogenic immune cells were isolated from draining lymph nodes of rats that were immunized with myelin basic protein (MBP) and complete Freund's adjuvant (CFA) and treated with EP in vivo. EP modulated cytokine generation in the examined cells in an anti-inflammatory manner: (i) the production of interleukin (IL)-6, but not IL-10, was down-regulated in astrocytes; (ii) the release of IL-6, tumor necrosis factor and nitric oxide from microglial cells was reduced; (iii) the production of interferon-gamma and IL-17, but not IL-10, was suppressed in lymph node cells isolated from MBP + CFA-immunized rats. In addition, EP alleviated EAE course in rats, delaying the onset, shortening the relapse, and lowering clinical scores. These results imply that EP has a potency to inhibit inflammation in the CNS. While further studies on the effect of EP on the CNS inflammation are warranted, we believe that EP might be applicable and beneficial in MS treatment.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Effect of ethyl pyruvate on central nervous system inflammation",
pages = "224-223",
number = "1-2",
volume = "275",
doi = "10.1016/j.jneuroim.2014.08.600"
}

Miljković, Đ., Petković, F., Blazevski, J., Momcilović, M., Djedović, N., Mostarica-Stojković, M.,& Spasojević, I.. (2014). Effect of ethyl pyruvate on central nervous system inflammation. in Journal of Neuroimmunology
Elsevier Science Bv, Amsterdam., 275(1-2), 223-224.
https://doi.org/10.1016/j.jneuroim.2014.08.600

Miljković Đ, Petković F, Blazevski J, Momcilović M, Djedović N, Mostarica-Stojković M, Spasojević I. Effect of ethyl pyruvate on central nervous system inflammation. in Journal of Neuroimmunology. 2014;275(1-2):223-224.
doi:10.1016/j.jneuroim.2014.08.600 .

Miljković, Đorđe, Petković, Filip, Blazevski, Jana, Momcilović, Miljana, Djedović, Neda, Mostarica-Stojković, Marija, Spasojević, Ivan, "Effect of ethyl pyruvate on central nervous system inflammation" in Journal of Neuroimmunology, 275, no. 1-2 (2014):223-224,
https://doi.org/10.1016/j.jneuroim.2014.08.600 . .

TY  - JOUR
AU  - Harhaji-Trajković, Ljubica M.
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Stojanović, Ivana D.
AU  - Momcilović, Miljana
AU  - Tufegdžić, Srđan J.
AU  - Maksimović, Vuk
AU  - Marjanović, Žaklina
AU  - Stosic-Grujicic, Stanislava
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/347
AB  - Anticancer activities of various extracts of the medicinal mushroom, Ganoderma lucidum, have been widely demonstrated and are mainly associated with the presence of different bioactive polysaccharides and triterpenoids. We have evaluated and compared in vitro and in vivo the antitumor effects of two preparations from Ganoderma lucidum: a methanol extract containing total terpenoids (GLme) and a purified methanol extract containing mainly acidic terpenoids (GLpme). Both extracts inhibited tumor growth of B16 mouse melanoma cells inoculated subcutaneously into syngeneic C57BL/6 mice and reduced viability of B16 cells in vitro, whereby GLme exhibited stronger effect. Furthermore, anticancer activity of GLme was demonstrated for the first time against two other rodent tumor cell lines, L929-mouse fibrosarcoma and C6-rat astrocytoma. The mechanism of antitumor activity of GLme comprised inhibition of cell proliferation and induction of caspase-dependent apoptotic cell death mediated by upregulated p53 and inhibited Bcl-2 expression. Moreover, the antitumor effect of the GLme was associated with intensified production of reactive oxygen species, whereas their neutralization by the antioxidant, N-acetyl cysteine, resulted in partial recovery of cell viability. Thus, our results suggest that GLme might be a good candidate for treatment of diverse forms of cancers.
PB  - Routledge Journals, Taylor & Francis Ltd, Abingdon
T2  - Nutrition and Cancer-An International Journal
T1  - Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo
EP  - 707
IS  - 5
SP  - 696
VL  - 61
DO  - 10.1080/01635580902898743
ER  - 

@article{
author = "Harhaji-Trajković, Ljubica M. and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, Ivana D. and Momcilović, Miljana and Tufegdžić, Srđan J. and Maksimović, Vuk and Marjanović, Žaklina and Stosic-Grujicic, Stanislava",
year = "2009",
abstract = "Anticancer activities of various extracts of the medicinal mushroom, Ganoderma lucidum, have been widely demonstrated and are mainly associated with the presence of different bioactive polysaccharides and triterpenoids. We have evaluated and compared in vitro and in vivo the antitumor effects of two preparations from Ganoderma lucidum: a methanol extract containing total terpenoids (GLme) and a purified methanol extract containing mainly acidic terpenoids (GLpme). Both extracts inhibited tumor growth of B16 mouse melanoma cells inoculated subcutaneously into syngeneic C57BL/6 mice and reduced viability of B16 cells in vitro, whereby GLme exhibited stronger effect. Furthermore, anticancer activity of GLme was demonstrated for the first time against two other rodent tumor cell lines, L929-mouse fibrosarcoma and C6-rat astrocytoma. The mechanism of antitumor activity of GLme comprised inhibition of cell proliferation and induction of caspase-dependent apoptotic cell death mediated by upregulated p53 and inhibited Bcl-2 expression. Moreover, the antitumor effect of the GLme was associated with intensified production of reactive oxygen species, whereas their neutralization by the antioxidant, N-acetyl cysteine, resulted in partial recovery of cell viability. Thus, our results suggest that GLme might be a good candidate for treatment of diverse forms of cancers.",
publisher = "Routledge Journals, Taylor & Francis Ltd, Abingdon",
journal = "Nutrition and Cancer-An International Journal",
title = "Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo",
pages = "707-696",
number = "5",
volume = "61",
doi = "10.1080/01635580902898743"
}

Harhaji-Trajković, L. M., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I. D., Momcilović, M., Tufegdžić, S. J., Maksimović, V., Marjanović, Ž.,& Stosic-Grujicic, S.. (2009). Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo. in Nutrition and Cancer-An International Journal
Routledge Journals, Taylor & Francis Ltd, Abingdon., 61(5), 696-707.
https://doi.org/10.1080/01635580902898743

Harhaji-Trajković LM, Mijatović S, Maksimović-Ivanić D, Stojanović ID, Momcilović M, Tufegdžić SJ, Maksimović V, Marjanović Ž, Stosic-Grujicic S. Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo. in Nutrition and Cancer-An International Journal. 2009;61(5):696-707.
doi:10.1080/01635580902898743 .

Harhaji-Trajković, Ljubica M., Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, Ivana D., Momcilović, Miljana, Tufegdžić, Srđan J., Maksimović, Vuk, Marjanović, Žaklina, Stosic-Grujicic, Stanislava, "Anticancer Properties of Ganoderma Lucidum Methanol Extracts In Vitro and In Vivo" in Nutrition and Cancer-An International Journal, 61, no. 5 (2009):696-707,
https://doi.org/10.1080/01635580902898743 . .

TY  - JOUR
AU  - Harhaji, Lj.
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Stojanović, I.
AU  - Momcilović, Miljana
AU  - Maksimović, Vuk
AU  - Tufegdžić, Srđan J.
AU  - Marjanović, Žaklina
AU  - Mostarica-Stojković, M.
AU  - Vučinić, Željko
AU  - Stosic-Grujicic, Stanislava
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/262
AB  - Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study
EP  - 1833
IS  - 5
SP  - 1825
VL  - 46
DO  - 10.1016/j.fct.2008.01.027
ER  - 

@article{
author = "Harhaji, Lj. and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, I. and Momcilović, Miljana and Maksimović, Vuk and Tufegdžić, Srđan J. and Marjanović, Žaklina and Mostarica-Stojković, M. and Vučinić, Željko and Stosic-Grujicic, Stanislava",
year = "2008",
abstract = "Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study",
pages = "1833-1825",
number = "5",
volume = "46",
doi = "10.1016/j.fct.2008.01.027"
}

Harhaji, Lj., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I., Momcilović, M., Maksimović, V., Tufegdžić, S. J., Marjanović, Ž., Mostarica-Stojković, M., Vučinić, Ž.,& Stosic-Grujicic, S.. (2008). Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 46(5), 1825-1833.
https://doi.org/10.1016/j.fct.2008.01.027

Harhaji L, Mijatović S, Maksimović-Ivanić D, Stojanović I, Momcilović M, Maksimović V, Tufegdžić SJ, Marjanović Ž, Mostarica-Stojković M, Vučinić Ž, Stosic-Grujicic S. Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology. 2008;46(5):1825-1833.
doi:10.1016/j.fct.2008.01.027 .

Harhaji, Lj., Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, I., Momcilović, Miljana, Maksimović, Vuk, Tufegdžić, Srđan J., Marjanović, Žaklina, Mostarica-Stojković, M., Vučinić, Željko, Stosic-Grujicic, Stanislava, "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study" in Food and Chemical Toxicology, 46, no. 5 (2008):1825-1833,
https://doi.org/10.1016/j.fct.2008.01.027 . .