TY  - JOUR
AU  - Vojvodić, Snežana
AU  - Dimitrijević, Milena
AU  - Žižić, Milan
AU  - Dučić, Tanja
AU  - AQUILANTI, Giuliana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Danilović Luković, Jelena
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Morina, Arian
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2378
AB  - Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.
PB  - Oxford University Press
T2  - Journal of Experimental Botany
T1  - A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana
EP  - 1122
IS  - 3
SP  - 1107
VL  - 74
DO  - 10.1093/jxb/erac472
ER  - 

@article{
author = "Vojvodić, Snežana and Dimitrijević, Milena and Žižić, Milan and Dučić, Tanja and AQUILANTI, Giuliana and Stanić, Marina and Zechmann, Bernd and Danilović Luković, Jelena and Stanković, Dalibor M. and Opačić, Miloš and Morina, Arian and Pittman, Jon K. and Spasojević, Ivan",
year = "2023",
abstract = "Metabolism of metals in microalgae and adaptation to metal excess are of significant environmental importance. We report a three-step mechanism that the green microalga Chlorella sorokiniana activates during the acquisition of and adaptation to manganese (Mn), which is both an essential trace metal and a pollutant of waters. In the early stage, Mn2+ was mainly bound to membrane phospholipids and phosphates in released mucilage. The outer cell wall
was reorganized and lipids were accumulated, with a relative increase in lipid saturation. Intracellular redox settings were rapidly altered in the presence of Mn excess, with increased production of reactive oxygen species that resulted in lipid peroxidation and a decrease in the concentration of thiols. In the later stage, Mn2+ was chelated by polyphosphates and accumulated in the cells. The structure of the inner cell wall was modified and the redox milieu established a new balance. Polyphosphates serve as a transient Mn2+ storage ligand, as proposed previously. In the final stage, Mn was stored in multivalent Mn clusters that resemble the structure of the tetramanganese–calcium core of the oxygen-evolving complex. The present findings elucidate the bioinorganic chemistry and metabolism of Mn in microalgae, and may shed new light on water-splitting Mn clusters.",
publisher = "Oxford University Press",
journal = "Journal of Experimental Botany",
title = "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana",
pages = "1122-1107",
number = "3",
volume = "74",
doi = "10.1093/jxb/erac472"
}

Vojvodić, S., Dimitrijević, M., Žižić, M., Dučić, T., AQUILANTI, G., Stanić, M., Zechmann, B., Danilović Luković, J., Stanković, D. M., Opačić, M., Morina, A., Pittman, J. K.,& Spasojević, I.. (2023). A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany
Oxford University Press., 74(3), 1107-1122.
https://doi.org/10.1093/jxb/erac472

Vojvodić S, Dimitrijević M, Žižić M, Dučić T, AQUILANTI G, Stanić M, Zechmann B, Danilović Luković J, Stanković DM, Opačić M, Morina A, Pittman JK, Spasojević I. A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana. in Journal of Experimental Botany. 2023;74(3):1107-1122.
doi:10.1093/jxb/erac472 .

Vojvodić, Snežana, Dimitrijević, Milena, Žižić, Milan, Dučić, Tanja, AQUILANTI, Giuliana, Stanić, Marina, Zechmann, Bernd, Danilović Luković, Jelena, Stanković, Dalibor M., Opačić, Miloš, Morina, Arian, Pittman, Jon K., Spasojević, Ivan, "A three-step process of manganese acquisition and storage in the microalga Chlorella sorokiniana" in Journal of Experimental Botany, 74, no. 3 (2023):1107-1122,
https://doi.org/10.1093/jxb/erac472 . .

TY  - CONF
AU  - Vojvodić, Snežana
AU  - Stanić, Marina
AU  - Zechmann, Bernd
AU  - Dimitrijević, Milena
AU  - Opačić, Miloš
AU  - Danilović Luković, Jelena
AU  - Morina, Arian
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2420
AB  - Many neutrophilic and acidophilic microalgal species tolerate high metal concentrations
and can survive or colonize metal-polluted waters. They show significant biotechnological
potential for the remediation and wastewaters processing. On the other hand, negative effects
of metal pollution on microalgae may affect the function of aquatic ecosystems because these
photosynthetic microorganisms represent the primary producers of O2 and biomass. However,
adaptive mechanisms that microalgae employ to detoxify metal excess are largely unknown.
Herein we analyzed the response of the freshwater microalga Chlorella sorokiniana to high
but non-toxic levels of Mn2+. Manganese is a key metal pollutant, with five possible oxidation
forms that can bind to a variety of different ligands. At pH below 7, it is predominantly present in
Mn2+ form. Scanning electron microscopy showed that in response to 1 mM Mn2+, C. sorokiniana
released mucilage polymers within 1 h. Electron paramagnetic resonance spectroscopy (EPR)
showed that the early response involved loose Mn2+ binding to mucilage and/or the cell wall.
The amount of loosely bound Mn2+ was significantly decreased after 24 h, whereas biomass
showed significant accumulation of Mn, O and P, as determined by energy dispersive X-ray
spectrometry, indicating the production of polyphosphates, which may sequester Mn. Further,
it was found that the exposure to Mn2+ resulted in rapid and transient decrease of total free
glutathione concentration; the drop was observed after 1 h, and the concentration returned to
initial values after 24 h. EPR measurements showed a similar trend in the level of reduced thiols.
The observed changes can be explained either by the synthesis of phytochelatins – sulfurrich
short-chain peptides that sequester metals, or by glutathionylation of proteins. Reduced
thiols could not be detected in the extracellular space, indicating that C. sorokiniana did not
release thiols in response to high Mn. These results demonstrate that the adaptive response
of C. sorokiniana to high Mn levels involves multiple components and time phases. The early
phase involves mucilage release, phytochelatins and/or protection of protein thiols, whereas
the successive phase involves Mn coordination by polyphosphates and other mechanisms that
remain to be resolved.
PB  - Elsevier
PB  - Society for Free Radical Research Europe (SFRR-E)
PB  - Ministry of Education, Science and Technological Development (Republic of Serbia)
PB  - Oxygen Club of California
C3  - Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts
T1  - Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana
EP  - 145
SP  - 145
DO  - 10.1016/j.freeradbiomed.2021.08.148
ER  - 

@conference{
author = "Vojvodić, Snežana and Stanić, Marina and Zechmann, Bernd and Dimitrijević, Milena and Opačić, Miloš and Danilović Luković, Jelena and Morina, Arian and Pittman, Jon K. and Spasojević, Ivan",
year = "2021",
abstract = "Many neutrophilic and acidophilic microalgal species tolerate high metal concentrations
and can survive or colonize metal-polluted waters. They show significant biotechnological
potential for the remediation and wastewaters processing. On the other hand, negative effects
of metal pollution on microalgae may affect the function of aquatic ecosystems because these
photosynthetic microorganisms represent the primary producers of O2 and biomass. However,
adaptive mechanisms that microalgae employ to detoxify metal excess are largely unknown.
Herein we analyzed the response of the freshwater microalga Chlorella sorokiniana to high
but non-toxic levels of Mn2+. Manganese is a key metal pollutant, with five possible oxidation
forms that can bind to a variety of different ligands. At pH below 7, it is predominantly present in
Mn2+ form. Scanning electron microscopy showed that in response to 1 mM Mn2+, C. sorokiniana
released mucilage polymers within 1 h. Electron paramagnetic resonance spectroscopy (EPR)
showed that the early response involved loose Mn2+ binding to mucilage and/or the cell wall.
The amount of loosely bound Mn2+ was significantly decreased after 24 h, whereas biomass
showed significant accumulation of Mn, O and P, as determined by energy dispersive X-ray
spectrometry, indicating the production of polyphosphates, which may sequester Mn. Further,
it was found that the exposure to Mn2+ resulted in rapid and transient decrease of total free
glutathione concentration; the drop was observed after 1 h, and the concentration returned to
initial values after 24 h. EPR measurements showed a similar trend in the level of reduced thiols.
The observed changes can be explained either by the synthesis of phytochelatins – sulfurrich
short-chain peptides that sequester metals, or by glutathionylation of proteins. Reduced
thiols could not be detected in the extracellular space, indicating that C. sorokiniana did not
release thiols in response to high Mn. These results demonstrate that the adaptive response
of C. sorokiniana to high Mn levels involves multiple components and time phases. The early
phase involves mucilage release, phytochelatins and/or protection of protein thiols, whereas
the successive phase involves Mn coordination by polyphosphates and other mechanisms that
remain to be resolved.",
publisher = "Elsevier, Society for Free Radical Research Europe (SFRR-E), Ministry of Education, Science and Technological Development (Republic of Serbia), Oxygen Club of California",
journal = "Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts",
title = "Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana",
pages = "145-145",
doi = "10.1016/j.freeradbiomed.2021.08.148"
}

Vojvodić, S., Stanić, M., Zechmann, B., Dimitrijević, M., Opačić, M., Danilović Luković, J., Morina, A., Pittman, J. K.,& Spasojević, I.. (2021). Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana. in Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts
Elsevier., 145-145.
https://doi.org/10.1016/j.freeradbiomed.2021.08.148

Vojvodić S, Stanić M, Zechmann B, Dimitrijević M, Opačić M, Danilović Luković J, Morina A, Pittman JK, Spasojević I. Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana. in Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts. 2021;:145-145.
doi:10.1016/j.freeradbiomed.2021.08.148 .

Vojvodić, Snežana, Stanić, Marina, Zechmann, Bernd, Dimitrijević, Milena, Opačić, Miloš, Danilović Luković, Jelena, Morina, Arian, Pittman, Jon K., Spasojević, Ivan, "Mechanisms of detoxification of high manganese concentrations by the microalga Chlorella sorokiniana" in Redox Biology in the 21st Century: A New Scientific Discipline, Meeting Abstracts (2021):145-145,
https://doi.org/10.1016/j.freeradbiomed.2021.08.148 . .

TY  - CONF
AU  - Romanović, Mima
AU  - Stanić, Marina
AU  - Bogdanović Pristov, Jelena
AU  - Morina, Arian
AU  - Dimitrijević, Milena
AU  - Pittman, Jon K.
AU  - Spasojević, Ivan
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2415
AB  - The impact of ionizing radiation on microalgae represents an important biotechnological and environmental issue. However, it has not been sufficiently investigated. Herein, we analyzed the effects of lowdose X-radiation on the growth, lipid production, and chlorophyll (Chl) and carotenoids content in Chlorella sorokiniana (CCAP 211/8K), which is both, a model and biotechnologically relevant species. C. sorokiniana culture was grown in 3N-BBM+V medium, at 22°C with a continuous photon flux of 120 μmol m−2 s−1. X-ray irradiation was applied in the early exponential phase of growth, at different doses (1, 2, 5, 10, 20 Gy) and rates (0.06, 0.24, 0.55 Gy/min). Parameters were monitored for 30 days. The exposure to 2 Gy and 5 Gy had a positive impact on biomass production. Dry weight was significantly higher in treated cultures than controls at days 25 and 30. Total lipid content (according to Nile Red fluorescence assay) was increased at day 30 in cultures exposed to 1 Gy (0.06 Gy/min) and 5 Gy (0.24 Gy/min). Chl content was increased for these doses in the exponential phase of growth. Chl b and carotenoids content was not significantly affected by irradiation. It is noteworthy that higher doses (10 and 20 Gy) had suppressing effects on growth and lipid production. The positive effects of ionizing radiation on biomass and lipid production can be attributed to the phenomenon of radiation hormesis (beneficial effects of low dose radiation on different biological parameters). Radiation hormesis has been shown previously documented on a number of plant species, and can be potentially employed in microalgae industry. On the other hand, microalgae are exposed to increased levels of ionizing irradiation in aquatic systems that are infested with radionuclides either naturally or by anthropogenic activity. Our results may add to the understanding of eco-physiology of
microalgae in such systems.
PB  - Taylor & Francis Group
C3  - European Journal of Phycology
T1  - The effects of ionizing irradiation on growth and lipid production in Chlorella sorokiniana
EP  - 165
IS  - Supp 1
SP  - 164
VL  - 54
DO  - 10.1080/09670262.2019.1626628
ER  - 

@conference{
author = "Romanović, Mima and Stanić, Marina and Bogdanović Pristov, Jelena and Morina, Arian and Dimitrijević, Milena and Pittman, Jon K. and Spasojević, Ivan",
year = "2019",
abstract = "The impact of ionizing radiation on microalgae represents an important biotechnological and environmental issue. However, it has not been sufficiently investigated. Herein, we analyzed the effects of lowdose X-radiation on the growth, lipid production, and chlorophyll (Chl) and carotenoids content in Chlorella sorokiniana (CCAP 211/8K), which is both, a model and biotechnologically relevant species. C. sorokiniana culture was grown in 3N-BBM+V medium, at 22°C with a continuous photon flux of 120 μmol m−2 s−1. X-ray irradiation was applied in the early exponential phase of growth, at different doses (1, 2, 5, 10, 20 Gy) and rates (0.06, 0.24, 0.55 Gy/min). Parameters were monitored for 30 days. The exposure to 2 Gy and 5 Gy had a positive impact on biomass production. Dry weight was significantly higher in treated cultures than controls at days 25 and 30. Total lipid content (according to Nile Red fluorescence assay) was increased at day 30 in cultures exposed to 1 Gy (0.06 Gy/min) and 5 Gy (0.24 Gy/min). Chl content was increased for these doses in the exponential phase of growth. Chl b and carotenoids content was not significantly affected by irradiation. It is noteworthy that higher doses (10 and 20 Gy) had suppressing effects on growth and lipid production. The positive effects of ionizing radiation on biomass and lipid production can be attributed to the phenomenon of radiation hormesis (beneficial effects of low dose radiation on different biological parameters). Radiation hormesis has been shown previously documented on a number of plant species, and can be potentially employed in microalgae industry. On the other hand, microalgae are exposed to increased levels of ionizing irradiation in aquatic systems that are infested with radionuclides either naturally or by anthropogenic activity. Our results may add to the understanding of eco-physiology of
microalgae in such systems.",
publisher = "Taylor & Francis Group",
journal = "European Journal of Phycology",
title = "The effects of ionizing irradiation on growth and lipid production in Chlorella sorokiniana",
pages = "165-164",
number = "Supp 1",
volume = "54",
doi = "10.1080/09670262.2019.1626628"
}

Romanović, M., Stanić, M., Bogdanović Pristov, J., Morina, A., Dimitrijević, M., Pittman, J. K.,& Spasojević, I.. (2019). The effects of ionizing irradiation on growth and lipid production in Chlorella sorokiniana. in European Journal of Phycology
Taylor & Francis Group., 54(Supp 1), 164-165.
https://doi.org/10.1080/09670262.2019.1626628

Romanović M, Stanić M, Bogdanović Pristov J, Morina A, Dimitrijević M, Pittman JK, Spasojević I. The effects of ionizing irradiation on growth and lipid production in Chlorella sorokiniana. in European Journal of Phycology. 2019;54(Supp 1):164-165.
doi:10.1080/09670262.2019.1626628 .

Romanović, Mima, Stanić, Marina, Bogdanović Pristov, Jelena, Morina, Arian, Dimitrijević, Milena, Pittman, Jon K., Spasojević, Ivan, "The effects of ionizing irradiation on growth and lipid production in Chlorella sorokiniana" in European Journal of Phycology, 54, no. Supp 1 (2019):164-165,
https://doi.org/10.1080/09670262.2019.1626628 . .

TY  - CONF
AU  - Jevtović (born Romanović), Mima
AU  - Danilović Luković, Jelena
AU  - Morina, Arian
AU  - Kovačević, Snežana
AU  - Stanić, Marina
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2812
AB  - Pozitivan biološki odgovor ćelije ili organizma na stres niskog intenziteta se naziva hormezis. U višim biljkama je hormezis kao odgovor na niske doze zračenja dobro dokumentovan ali ovakva ispitivanja do sada nisu rađena na mikroalgama. Mikroalge su grupa organizama koji se lako prilagođavaju nepovoljnim uslovima sredine i na taj način mogu proizvesti veliku biomasu pri niskom sadržaju nutrijenata.Cilj ove studije je bio da ispita odgovor jednoćelijske zelene alge Chlamydomonas reinhardtii (CCAP 11/32C) na niske doze X-zračenja. Ispitivan je efekat na produkciju biomase kao i na promene u zastupljenosti hlorofila a (Chl a) i b (Chl b) u različitim fazama rasta nakon izlaganja tretmanu. Alge su izlagane zračenju od 10; 20; 50 i 100 Gy pri brzinama od 0,565; 2,290 i 5,057 Gy/min. Tretman od 10 Gy je pri brzini od 0,565 Gy/min doveo do povećanja biomase za 21±15% 15 dana nakon tretmana, a pri brzini od 2,290 Gy/min do povećanja od 19±8% već nakon 5 dana. Tretman od 20 Gy je pri brzini od 0,565 Gy /min doveo do povećanja biomase od 22±12% nakon 15 dana. Svi ostali tretmani ili nisu imali efekta ili su doveli do smanjenja biomase. Meren je i efekat zračenja na sadržaj Chl a i Chl b (μg/mg suve mase). Sadržaj ovih pigmenata vremenom opada ali neki tretmani su doveli do smanjenja ovog efekta. Doza od 20 Gy pri brzini od 2,290 Gy/min je dala najbolje efekte na očuvanje Chl a i Chl b i oni su u odnosu na kontrolu bili veći 31±9% i 31±8%.
PB  - Srpsko biološko društvo
C3  - II Kongres biologa Srbije
T1  - Uticaj niskih doza X zračenja na parametre vijabilnosti jednoćelijske alge Chlamydomonas reinhardtii
EP  - 222
SP  - 222
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2812
ER  - 

@conference{
author = "Jevtović (born Romanović), Mima and Danilović Luković, Jelena and Morina, Arian and Kovačević, Snežana and Stanić, Marina and Spasojević, Ivan",
year = "2018",
abstract = "Pozitivan biološki odgovor ćelije ili organizma na stres niskog intenziteta se naziva hormezis. U višim biljkama je hormezis kao odgovor na niske doze zračenja dobro dokumentovan ali ovakva ispitivanja do sada nisu rađena na mikroalgama. Mikroalge su grupa organizama koji se lako prilagođavaju nepovoljnim uslovima sredine i na taj način mogu proizvesti veliku biomasu pri niskom sadržaju nutrijenata.Cilj ove studije je bio da ispita odgovor jednoćelijske zelene alge Chlamydomonas reinhardtii (CCAP 11/32C) na niske doze X-zračenja. Ispitivan je efekat na produkciju biomase kao i na promene u zastupljenosti hlorofila a (Chl a) i b (Chl b) u različitim fazama rasta nakon izlaganja tretmanu. Alge su izlagane zračenju od 10; 20; 50 i 100 Gy pri brzinama od 0,565; 2,290 i 5,057 Gy/min. Tretman od 10 Gy je pri brzini od 0,565 Gy/min doveo do povećanja biomase za 21±15% 15 dana nakon tretmana, a pri brzini od 2,290 Gy/min do povećanja od 19±8% već nakon 5 dana. Tretman od 20 Gy je pri brzini od 0,565 Gy /min doveo do povećanja biomase od 22±12% nakon 15 dana. Svi ostali tretmani ili nisu imali efekta ili su doveli do smanjenja biomase. Meren je i efekat zračenja na sadržaj Chl a i Chl b (μg/mg suve mase). Sadržaj ovih pigmenata vremenom opada ali neki tretmani su doveli do smanjenja ovog efekta. Doza od 20 Gy pri brzini od 2,290 Gy/min je dala najbolje efekte na očuvanje Chl a i Chl b i oni su u odnosu na kontrolu bili veći 31±9% i 31±8%.",
publisher = "Srpsko biološko društvo",
journal = "II Kongres biologa Srbije",
title = "Uticaj niskih doza X zračenja na parametre vijabilnosti jednoćelijske alge Chlamydomonas reinhardtii",
pages = "222-222",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2812"
}

Jevtović (born Romanović), M., Danilović Luković, J., Morina, A., Kovačević, S., Stanić, M.,& Spasojević, I.. (2018). Uticaj niskih doza X zračenja na parametre vijabilnosti jednoćelijske alge Chlamydomonas reinhardtii. in II Kongres biologa Srbije
Srpsko biološko društvo., 222-222.
https://hdl.handle.net/21.15107/rcub_rimsi_2812

Jevtović (born Romanović) M, Danilović Luković J, Morina A, Kovačević S, Stanić M, Spasojević I. Uticaj niskih doza X zračenja na parametre vijabilnosti jednoćelijske alge Chlamydomonas reinhardtii. in II Kongres biologa Srbije. 2018;:222-222.
https://hdl.handle.net/21.15107/rcub_rimsi_2812 .

Jevtović (born Romanović), Mima, Danilović Luković, Jelena, Morina, Arian, Kovačević, Snežana, Stanić, Marina, Spasojević, Ivan, "Uticaj niskih doza X zračenja na parametre vijabilnosti jednoćelijske alge Chlamydomonas reinhardtii" in II Kongres biologa Srbije (2018):222-222,
https://hdl.handle.net/21.15107/rcub_rimsi_2812 .

TY  - JOUR
AU  - Morina, Arian
AU  - Morina, Filis
AU  - Đikanović, Vesna
AU  - Spasić, Slađana
AU  - Krpo-Cetković, Jasmina
AU  - Lenhardt, Mirjana
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/965
AB  - The main objective of this study was to evaluate the concentrations and seasonal variations of trace elements in surface sediments of three major rivers in Serbia-the Danube, the Zapadna Morava (ZM), and the JuA3/4na Morava (JM)-according to sediment quality guidelines. The ZM and the JM create the Velika Morava River, one of the most important tributaries of the Danube, which has been characterized as a source of heavy metal pollution. The total concentrations of 15 elements (Al, As, B, Ba, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Sr, and Zn) were determined in surface sediments (0-15 cm depth) collected during three seasons using inductively coupled plasma spectroscopy (ICP-OES). Principle component analysis (PCA) was used to identify the main variations in metal concentrations and grain size distribution. Scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM-EDS) was used for grain analysis. PCA and three-way MANOVA results showed significant differences in element concentrations and grain size distribution between the rivers, and significant seasonal differences for each river. The concentrations of Cu and Ni exceeded sediment quality guideline levels in the ZM and the Danube, respectively, while excess Hg was detected in all three rivers. Concentrations of Al, Ba, Cu, Fe, Sr, and Zn significantly varied between seasons in the Danube and the ZM, being the highest in the summer. In the JM, concentrations of Al, As, Fe, Mn, and Zn varied with season, with the lowest values in the summer. The ZM had the highest percentage of silt and clay, and SEM-EDS analysis of ZM sediments showed associations of Cu with carbonate hydroxides and/or iron oxides in particles  lt  100 mu m. The results suggested that mining and industrial activities could be the sources of increased levels of metals in the ZM. The sediments collected from the ZM were considerably more polluted with heavy metals in comparison to the JM. Cu was identified as a heavy metal of greatest risk in the ZM. The ZM was indicated as the main source of heavy metal delivery in the Velika Morava and Danube rivers. It is suggested that the main factors influencing pollution levels could be anthropogenic sources and industrial and mining activities, while seasonal changes might be related to dynamics of water flow and morphological characteristics of the two tributary rivers.
PB  - Springer Heidelberg, Heidelberg
T2  - Journal of Soils and Sediments
T1  - Seasonal variation in element concentrations in surface sediments of three rivers with different pollution input in Serbia
EP  - 265
IS  - 1
SP  - 255
VL  - 16
DO  - 10.1007/s11368-015-1211-6
ER  - 

@article{
author = "Morina, Arian and Morina, Filis and Đikanović, Vesna and Spasić, Slađana and Krpo-Cetković, Jasmina and Lenhardt, Mirjana",
year = "2016",
abstract = "The main objective of this study was to evaluate the concentrations and seasonal variations of trace elements in surface sediments of three major rivers in Serbia-the Danube, the Zapadna Morava (ZM), and the JuA3/4na Morava (JM)-according to sediment quality guidelines. The ZM and the JM create the Velika Morava River, one of the most important tributaries of the Danube, which has been characterized as a source of heavy metal pollution. The total concentrations of 15 elements (Al, As, B, Ba, Co, Cr, Cu, Fe, Hg, Mn, Mo, Ni, Pb, Sr, and Zn) were determined in surface sediments (0-15 cm depth) collected during three seasons using inductively coupled plasma spectroscopy (ICP-OES). Principle component analysis (PCA) was used to identify the main variations in metal concentrations and grain size distribution. Scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM-EDS) was used for grain analysis. PCA and three-way MANOVA results showed significant differences in element concentrations and grain size distribution between the rivers, and significant seasonal differences for each river. The concentrations of Cu and Ni exceeded sediment quality guideline levels in the ZM and the Danube, respectively, while excess Hg was detected in all three rivers. Concentrations of Al, Ba, Cu, Fe, Sr, and Zn significantly varied between seasons in the Danube and the ZM, being the highest in the summer. In the JM, concentrations of Al, As, Fe, Mn, and Zn varied with season, with the lowest values in the summer. The ZM had the highest percentage of silt and clay, and SEM-EDS analysis of ZM sediments showed associations of Cu with carbonate hydroxides and/or iron oxides in particles  lt  100 mu m. The results suggested that mining and industrial activities could be the sources of increased levels of metals in the ZM. The sediments collected from the ZM were considerably more polluted with heavy metals in comparison to the JM. Cu was identified as a heavy metal of greatest risk in the ZM. The ZM was indicated as the main source of heavy metal delivery in the Velika Morava and Danube rivers. It is suggested that the main factors influencing pollution levels could be anthropogenic sources and industrial and mining activities, while seasonal changes might be related to dynamics of water flow and morphological characteristics of the two tributary rivers.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Journal of Soils and Sediments",
title = "Seasonal variation in element concentrations in surface sediments of three rivers with different pollution input in Serbia",
pages = "265-255",
number = "1",
volume = "16",
doi = "10.1007/s11368-015-1211-6"
}

Morina, A., Morina, F., Đikanović, V., Spasić, S., Krpo-Cetković, J.,& Lenhardt, M.. (2016). Seasonal variation in element concentrations in surface sediments of three rivers with different pollution input in Serbia. in Journal of Soils and Sediments
Springer Heidelberg, Heidelberg., 16(1), 255-265.
https://doi.org/10.1007/s11368-015-1211-6

Morina A, Morina F, Đikanović V, Spasić S, Krpo-Cetković J, Lenhardt M. Seasonal variation in element concentrations in surface sediments of three rivers with different pollution input in Serbia. in Journal of Soils and Sediments. 2016;16(1):255-265.
doi:10.1007/s11368-015-1211-6 .

Morina, Arian, Morina, Filis, Đikanović, Vesna, Spasić, Slađana, Krpo-Cetković, Jasmina, Lenhardt, Mirjana, "Seasonal variation in element concentrations in surface sediments of three rivers with different pollution input in Serbia" in Journal of Soils and Sediments, 16, no. 1 (2016):255-265,
https://doi.org/10.1007/s11368-015-1211-6 . .

TY  - JOUR
AU  - Morina, Arian
AU  - Morina, Filis
AU  - Đikanović, Vesna
AU  - Spasić, Slađana
AU  - Krpo-Cetković, Jasmina
AU  - Kostic, Bojan
AU  - Lenhardt, Mirjana
PY  - 2016
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/942
AB  - River sediments are a major source of metal contamination in aquatic food webs. Due to the ability of metals to move up the food chain, fishes, occupying higher trophic levels, are considered to be good environmental indicators of metal pollution. The aim of this study was to analyze the metal content in tissues of the common barbel (Barbus barbus), a rheophilous cyprinid fish widely distributed in the Danube Basin, in order to find out if it can be used as a bioindicator of the metal content in the river sediment. We analyzed bioavailable concentrations of 15 elements (Al, As, B, Ba, Co, Cr, Cu, Hg, Mn, Mo, Ni, Pb, Se, Sr, and Zn) in sediments of the Danube (D), the Zapadna Morava (ZM), and the JuA3/4na Morava (JM) using the inductively coupled plasma spectroscopy (ICP-OES). The barbel specimens were collected in the proximity of sediment sampling sites for the analysis of metals in four tissues, gills, muscle, intestine, and liver. The sediment analysis indicated that the ZM is the most polluted with Cu, Ni, and Zn compared to other two rivers. The JM had the lowest concentrations of almost all observed elements, while the Danube sediments were mainly characterized by higher concentrations of Pb. The fish from the ZM had the highest concentration of Cu and Ni in the liver and intestine, and of Zn in the muscle tissue, which was in accordance with the concentrations of these metals in the sediment. Scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM-EDS) was used for further analyses of metal interactions with fish tissues. The results suggest that the barbel can potentially be used as a bioindicator of sediment quality with respect to metal contamination.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Common barbel (Barbus barbus) as a bioindicator of surface river sediment pollution with Cu and Zn in three rivers of the Danube River Basin in Serbia
EP  - 6734
IS  - 7
SP  - 6723
VL  - 23
DO  - 10.1007/s11356-015-5901-9
ER  - 

@article{
author = "Morina, Arian and Morina, Filis and Đikanović, Vesna and Spasić, Slađana and Krpo-Cetković, Jasmina and Kostic, Bojan and Lenhardt, Mirjana",
year = "2016",
abstract = "River sediments are a major source of metal contamination in aquatic food webs. Due to the ability of metals to move up the food chain, fishes, occupying higher trophic levels, are considered to be good environmental indicators of metal pollution. The aim of this study was to analyze the metal content in tissues of the common barbel (Barbus barbus), a rheophilous cyprinid fish widely distributed in the Danube Basin, in order to find out if it can be used as a bioindicator of the metal content in the river sediment. We analyzed bioavailable concentrations of 15 elements (Al, As, B, Ba, Co, Cr, Cu, Hg, Mn, Mo, Ni, Pb, Se, Sr, and Zn) in sediments of the Danube (D), the Zapadna Morava (ZM), and the JuA3/4na Morava (JM) using the inductively coupled plasma spectroscopy (ICP-OES). The barbel specimens were collected in the proximity of sediment sampling sites for the analysis of metals in four tissues, gills, muscle, intestine, and liver. The sediment analysis indicated that the ZM is the most polluted with Cu, Ni, and Zn compared to other two rivers. The JM had the lowest concentrations of almost all observed elements, while the Danube sediments were mainly characterized by higher concentrations of Pb. The fish from the ZM had the highest concentration of Cu and Ni in the liver and intestine, and of Zn in the muscle tissue, which was in accordance with the concentrations of these metals in the sediment. Scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM-EDS) was used for further analyses of metal interactions with fish tissues. The results suggest that the barbel can potentially be used as a bioindicator of sediment quality with respect to metal contamination.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Common barbel (Barbus barbus) as a bioindicator of surface river sediment pollution with Cu and Zn in three rivers of the Danube River Basin in Serbia",
pages = "6734-6723",
number = "7",
volume = "23",
doi = "10.1007/s11356-015-5901-9"
}

Morina, A., Morina, F., Đikanović, V., Spasić, S., Krpo-Cetković, J., Kostic, B.,& Lenhardt, M.. (2016). Common barbel (Barbus barbus) as a bioindicator of surface river sediment pollution with Cu and Zn in three rivers of the Danube River Basin in Serbia. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 23(7), 6723-6734.
https://doi.org/10.1007/s11356-015-5901-9

Morina A, Morina F, Đikanović V, Spasić S, Krpo-Cetković J, Kostic B, Lenhardt M. Common barbel (Barbus barbus) as a bioindicator of surface river sediment pollution with Cu and Zn in three rivers of the Danube River Basin in Serbia. in Environmental Science and Pollution Research. 2016;23(7):6723-6734.
doi:10.1007/s11356-015-5901-9 .

Morina, Arian, Morina, Filis, Đikanović, Vesna, Spasić, Slađana, Krpo-Cetković, Jasmina, Kostic, Bojan, Lenhardt, Mirjana, "Common barbel (Barbus barbus) as a bioindicator of surface river sediment pollution with Cu and Zn in three rivers of the Danube River Basin in Serbia" in Environmental Science and Pollution Research, 23, no. 7 (2016):6723-6734,
https://doi.org/10.1007/s11356-015-5901-9 . .

TY  - JOUR
AU  - Andrades, Michael Everton
AU  - Morina, Arian
AU  - Spasic, Snežana D
AU  - Spasojević, Ivan
PY  - 2011
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/480
AB  - The pathogenesis of sepsis and its progression to multiple organ dysfunction syndrome and septic shock have been the subject of investigations for nearly half a century. Controversies still exist with regard to understanding the molecular pathophysiology of sepsis in relation to the complex roles played by reactive oxygen species, nitric oxide, complements and cytokines. In the present review we categorise the key turning points in sepsis development and outline the most probable sequence of events leading to cellular dysfunction and organ failure under septic conditions. We have applied an integrative approach in order to fuse current state-of-the-art knowledge about redox processes involving hydrogen peroxide, nitric oxide, superoxide, peroxynitrite and hydroxyl radical, which lead to mitochondrial respiratory dysfunction. Finally, from this point of view, the potential of redox therapy targeting sepsis is discussed.
PB  - Bmc, London
T2  - Critical Care
T1  - Bench-to-bedside review: Sepsis - from the redox point of view
IS  - 5
VL  - 15
DO  - 10.1186/cc10334
ER  - 

@article{
author = "Andrades, Michael Everton and Morina, Arian and Spasic, Snežana D and Spasojević, Ivan",
year = "2011",
abstract = "The pathogenesis of sepsis and its progression to multiple organ dysfunction syndrome and septic shock have been the subject of investigations for nearly half a century. Controversies still exist with regard to understanding the molecular pathophysiology of sepsis in relation to the complex roles played by reactive oxygen species, nitric oxide, complements and cytokines. In the present review we categorise the key turning points in sepsis development and outline the most probable sequence of events leading to cellular dysfunction and organ failure under septic conditions. We have applied an integrative approach in order to fuse current state-of-the-art knowledge about redox processes involving hydrogen peroxide, nitric oxide, superoxide, peroxynitrite and hydroxyl radical, which lead to mitochondrial respiratory dysfunction. Finally, from this point of view, the potential of redox therapy targeting sepsis is discussed.",
publisher = "Bmc, London",
journal = "Critical Care",
title = "Bench-to-bedside review: Sepsis - from the redox point of view",
number = "5",
volume = "15",
doi = "10.1186/cc10334"
}

Andrades, M. E., Morina, A., Spasic, S. D.,& Spasojević, I.. (2011). Bench-to-bedside review: Sepsis - from the redox point of view. in Critical Care
Bmc, London., 15(5).
https://doi.org/10.1186/cc10334

Andrades ME, Morina A, Spasic SD, Spasojević I. Bench-to-bedside review: Sepsis - from the redox point of view. in Critical Care. 2011;15(5).
doi:10.1186/cc10334 .

Andrades, Michael Everton, Morina, Arian, Spasic, Snežana D, Spasojević, Ivan, "Bench-to-bedside review: Sepsis - from the redox point of view" in Critical Care, 15, no. 5 (2011),
https://doi.org/10.1186/cc10334 . .

TY  - JOUR
AU  - Spasojević, Ivan
AU  - Mojović, Miloš
AU  - Stević, Zorica D
AU  - Spasic, Snežana D
AU  - Jones, David R
AU  - Morina, Arian
AU  - Spasić, Mihajlo
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/417
AB  - A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.
PB  - Maney Publishing, Leeds
T2  - Redox Report
T1  - Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid
EP  - 35
IS  - 1
SP  - 29
VL  - 15
DO  - 10.1179/174329210X12650506623087
ER  - 

@article{
author = "Spasojević, Ivan and Mojović, Miloš and Stević, Zorica D and Spasic, Snežana D and Jones, David R and Morina, Arian and Spasić, Mihajlo",
year = "2010",
abstract = "A breakdown in homeostasis of redox-active metals represents an important factor for neurodegeneration. We have used EPR spectroscopy and BMPO spin-trap to investigate the catalytic properties and ligand modulation of redox activity of copper and iron in human cerebrospinal fluid (CSF). In contrast to iron, copper supplementation provoked a statistically significant increase in hydroxyl free radical generation in CSF treated with H(2)O(2). However, in a binary copper/iron containing Fenton system, iron catalytically activated copper. The chelator EDTA, which represents a model of physiological metal ligands, completely prevented copper's redox activity in CSF, while iron chelation led to a significant increase in hydroxyl radical generation, indicating that copper and iron do not only have diverse catalytic properties in the CSF but also that their redox activities are differently modulated by ligands. The application of DDC reduced hydroxyl radical generation in the CSF containing catalytically active metals (free Cu(2+) or Fe(3+)-EDTA complex). We conclude that chelators, such as DDC, are capable of preventing the pro-oxidative activity of both metals and may be suitable for reducing hydroxyl radical formation in certain pathophysiological settings.",
publisher = "Maney Publishing, Leeds",
journal = "Redox Report",
title = "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid",
pages = "35-29",
number = "1",
volume = "15",
doi = "10.1179/174329210X12650506623087"
}

Spasojević, I., Mojović, M., Stević, Z. D., Spasic, S. D., Jones, D. R., Morina, A.,& Spasić, M.. (2010). Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report
Maney Publishing, Leeds., 15(1), 29-35.
https://doi.org/10.1179/174329210X12650506623087

Spasojević I, Mojović M, Stević ZD, Spasic SD, Jones DR, Morina A, Spasić M. Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid. in Redox Report. 2010;15(1):29-35.
doi:10.1179/174329210X12650506623087 .

Spasojević, Ivan, Mojović, Miloš, Stević, Zorica D, Spasic, Snežana D, Jones, David R, Morina, Arian, Spasić, Mihajlo, "Bioavailability and catalytic properties of copper and iron for Fenton chemistry in human cerebrospinal fluid" in Redox Report, 15, no. 1 (2010):29-35,
https://doi.org/10.1179/174329210X12650506623087 . .