TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Savic, Danijela
AU  - Korać Jačić, Jelena
AU  - Petkovic, Branka
AU  - Stojadinovic, Gordana
AU  - Martac, Ljiljana
AU  - Bogdanović Pristov, Jelena
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2010
AB  - Living cells exhibit multiple ion channel proteins and malfunction of these channels underlies 
numerous diseases. Channelopathies include diseases of the nervous, cardiovascular, respiratory, 
endocrine, urinary, and immune system. Currently, ion channels represent the second-largest target for existing drugs. Here, using the whole-cell patch-clamp technique, we explored the epinephrine effect on membrane ionic currents in glioma C6 cells. We demonstrate that epinephrine specifically evokes an increase of C6 cells outward ionic currents that is stable within 10 min, while it does not affect inward currents. Our results thus provide fine resolution and time frame for targeting ion channel activity that is crucial in pharmacological investigations.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)
T1  - MEMBRANE CURRENTS OF C6 CELLS IN PHARMACOLOGICAL  INVESTIGATION
EP  - 592
SP  - 589
VL  - 2
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2010
ER  - 

@conference{
author = "Nikolic, Ljiljana and Savic, Danijela and Korać Jačić, Jelena and Petkovic, Branka and Stojadinovic, Gordana and Martac, Ljiljana and Bogdanović Pristov, Jelena",
year = "2022",
abstract = "Living cells exhibit multiple ion channel proteins and malfunction of these channels underlies 
numerous diseases. Channelopathies include diseases of the nervous, cardiovascular, respiratory, 
endocrine, urinary, and immune system. Currently, ion channels represent the second-largest target for existing drugs. Here, using the whole-cell patch-clamp technique, we explored the epinephrine effect on membrane ionic currents in glioma C6 cells. We demonstrate that epinephrine specifically evokes an increase of C6 cells outward ionic currents that is stable within 10 min, while it does not affect inward currents. Our results thus provide fine resolution and time frame for targeting ion channel activity that is crucial in pharmacological investigations.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)",
title = "MEMBRANE CURRENTS OF C6 CELLS IN PHARMACOLOGICAL  INVESTIGATION",
pages = "592-589",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2010"
}

Nikolic, L., Savic, D., Korać Jačić, J., Petkovic, B., Stojadinovic, G., Martac, L.,& Bogdanović Pristov, J.. (2022). MEMBRANE CURRENTS OF C6 CELLS IN PHARMACOLOGICAL  INVESTIGATION. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)
Society of Physical Chemists of Serbia., 2, 589-592.
https://hdl.handle.net/21.15107/rcub_rimsi_2010

Nikolic L, Savic D, Korać Jačić J, Petkovic B, Stojadinovic G, Martac L, Bogdanović Pristov J. MEMBRANE CURRENTS OF C6 CELLS IN PHARMACOLOGICAL  INVESTIGATION. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings). 2022;2:589-592.
https://hdl.handle.net/21.15107/rcub_rimsi_2010 .

Nikolic, Ljiljana, Savic, Danijela, Korać Jačić, Jelena, Petkovic, Branka, Stojadinovic, Gordana, Martac, Ljiljana, Bogdanović Pristov, Jelena, "MEMBRANE CURRENTS OF C6 CELLS IN PHARMACOLOGICAL  INVESTIGATION" in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings), 2 (2022):589-592,
https://hdl.handle.net/21.15107/rcub_rimsi_2010 .

TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Vidović, Marija
AU  - Todorovic, Natasa
AU  - Petkovic, Branka
AU  - Stojadinovic, Gordana
AU  - Martac, Ljiljana
AU  - Bogdanović Pristov, Jelena
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2008
AB  - Investigating membrane properties of plants is a challenging task, considering that success of 
experiments is highly dependent on the possibility to isolate metabolically active protoplasts that 
can withstand membrane current recordings. The aim of the present work is to obtain viable 
protoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patch
clamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplasts 
with preserved membrane integrity suitable for electrophysiological experiments. We applied a 
custom approach for patch-clamping protoplasts using a microscope with a movable microscope 
stage. We recorded prominent inward and prominent outward types of membrane current profiles of protoplasts. Obtained data indicate that optimized isolation protocol and custom system for patch clamping, can be applied to study membrane properties of root protoplasts.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)
T1  - PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE
EP  - 274
SP  - 271
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2008
ER  - 

@conference{
author = "Nikolic, Ljiljana and Vidović, Marija and Todorovic, Natasa and Petkovic, Branka and Stojadinovic, Gordana and Martac, Ljiljana and Bogdanović Pristov, Jelena",
year = "2022",
abstract = "Investigating membrane properties of plants is a challenging task, considering that success of 
experiments is highly dependent on the possibility to isolate metabolically active protoplasts that 
can withstand membrane current recordings. The aim of the present work is to obtain viable 
protoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patch
clamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplasts 
with preserved membrane integrity suitable for electrophysiological experiments. We applied a 
custom approach for patch-clamping protoplasts using a microscope with a movable microscope 
stage. We recorded prominent inward and prominent outward types of membrane current profiles of protoplasts. Obtained data indicate that optimized isolation protocol and custom system for patch clamping, can be applied to study membrane properties of root protoplasts.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)",
title = "PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE",
pages = "274-271",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2008"
}

Nikolic, L., Vidović, M., Todorovic, N., Petkovic, B., Stojadinovic, G., Martac, L.,& Bogdanović Pristov, J.. (2022). PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)
Society of Physical Chemists of Serbia., 1, 271-274.
https://hdl.handle.net/21.15107/rcub_rimsi_2008

Nikolic L, Vidović M, Todorovic N, Petkovic B, Stojadinovic G, Martac L, Bogdanović Pristov J. PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings). 2022;1:271-274.
https://hdl.handle.net/21.15107/rcub_rimsi_2008 .

Nikolic, Ljiljana, Vidović, Marija, Todorovic, Natasa, Petkovic, Branka, Stojadinovic, Gordana, Martac, Ljiljana, Bogdanović Pristov, Jelena, "PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE" in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings), 1 (2022):271-274,
https://hdl.handle.net/21.15107/rcub_rimsi_2008 .

TY  - CONF
AU  - Milicevic, Katarina
AU  - Bijelic, Dunja
AU  - Lazarevic, Milica
AU  - Miljkovic, Djordje
AU  - Bogdanović Pristov, Jelena
AU  - Petkovic, Branka
AU  - Andjus, Pavle
AU  - Momcilovic, Miljana
AU  - Nikolic, Ljiljana
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2017
AB  - Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS), characterized by focal neurodegenerative and demyelinating lesions. A major contributor to the pathogenic process of MS is the complex interaction between astrocytes and the CNS-infiltrating immune cells (CNS-IIC). The aim of our study is to explore how naïve astrocytes respond to the autoreactive immune cells that invade the CNS. For this reason, CNS-IICs were isolated and purified from spinal cords of rats with experimental autoimmune encephalomyelitis. Ca2+ dynamics was monitored in Fluo-4 labeled naïve astrocytes, isolated from spinal cords of wild type rat pups, following brief bath application of CNS-IIC or peripheral immune cells, with different pharmacological agents. CNS-IICs, and not peripheral immune cells, induced robust elevation of intracellular Ca2+ in naïve astrocytes. We demonstrated that this CNS IIC-induced increase in astrocyte Ca2+ does not depend on the metabotropic glutamate receptors, metabotropic purinergic P2Y1 receptors or TRPA1 channels. Remarkably, further research showed that Ca2+ elevation in astrocytes upon exposure to CNS IICs is due to the activation of ionotropic purinergic P2X7 receptors. Bioluminescence assay showed that immune cell-derived ATP is not a cause of astrocytic P2X7 receptor activation. In fact, we showed that CNS-IICs promoted P2X7 receptor activation and increase in cytosolic Ca2+ in astrocytes by astrocytic hemichannel-dependent ATP release mechanism. Our data suggest that direct contact between astrocytes and CNS IICs induce ATP-dependent Ca2+ changes in astrocytes and points to the new aspect of cell-cell interactions in propagation of neuroinflammatory response in CNS autoimmunity.
PB  - Querétaro, México: Instituto de neurobiologia
C3  - Proceedings: 3rd Symposium on Physiology and pathology of neuroglia; 2020 Noc 24-25; Virtual. Querétaro, México: Instituto de neurobiologia
T1  - Central nervous system-infiltrated immune cells alter calcium dynamics in astrocytes
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2017
ER  - 

@conference{
author = "Milicevic, Katarina and Bijelic, Dunja and Lazarevic, Milica and Miljkovic, Djordje and Bogdanović Pristov, Jelena and Petkovic, Branka and Andjus, Pavle and Momcilovic, Miljana and Nikolic, Ljiljana",
year = "2020",
abstract = "Multiple sclerosis (MS) is a chronic inflammatory disease of the central nervous system (CNS), characterized by focal neurodegenerative and demyelinating lesions. A major contributor to the pathogenic process of MS is the complex interaction between astrocytes and the CNS-infiltrating immune cells (CNS-IIC). The aim of our study is to explore how naïve astrocytes respond to the autoreactive immune cells that invade the CNS. For this reason, CNS-IICs were isolated and purified from spinal cords of rats with experimental autoimmune encephalomyelitis. Ca2+ dynamics was monitored in Fluo-4 labeled naïve astrocytes, isolated from spinal cords of wild type rat pups, following brief bath application of CNS-IIC or peripheral immune cells, with different pharmacological agents. CNS-IICs, and not peripheral immune cells, induced robust elevation of intracellular Ca2+ in naïve astrocytes. We demonstrated that this CNS IIC-induced increase in astrocyte Ca2+ does not depend on the metabotropic glutamate receptors, metabotropic purinergic P2Y1 receptors or TRPA1 channels. Remarkably, further research showed that Ca2+ elevation in astrocytes upon exposure to CNS IICs is due to the activation of ionotropic purinergic P2X7 receptors. Bioluminescence assay showed that immune cell-derived ATP is not a cause of astrocytic P2X7 receptor activation. In fact, we showed that CNS-IICs promoted P2X7 receptor activation and increase in cytosolic Ca2+ in astrocytes by astrocytic hemichannel-dependent ATP release mechanism. Our data suggest that direct contact between astrocytes and CNS IICs induce ATP-dependent Ca2+ changes in astrocytes and points to the new aspect of cell-cell interactions in propagation of neuroinflammatory response in CNS autoimmunity.",
publisher = "Querétaro, México: Instituto de neurobiologia",
journal = "Proceedings: 3rd Symposium on Physiology and pathology of neuroglia; 2020 Noc 24-25; Virtual. Querétaro, México: Instituto de neurobiologia",
title = "Central nervous system-infiltrated immune cells alter calcium dynamics in astrocytes",
pages = "45",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2017"
}

Milicevic, K., Bijelic, D., Lazarevic, M., Miljkovic, D., Bogdanović Pristov, J., Petkovic, B., Andjus, P., Momcilovic, M.,& Nikolic, L.. (2020). Central nervous system-infiltrated immune cells alter calcium dynamics in astrocytes. in Proceedings: 3rd Symposium on Physiology and pathology of neuroglia; 2020 Noc 24-25; Virtual. Querétaro, México: Instituto de neurobiologia
Querétaro, México: Instituto de neurobiologia., 45.
https://hdl.handle.net/21.15107/rcub_rimsi_2017

Milicevic K, Bijelic D, Lazarevic M, Miljkovic D, Bogdanović Pristov J, Petkovic B, Andjus P, Momcilovic M, Nikolic L. Central nervous system-infiltrated immune cells alter calcium dynamics in astrocytes. in Proceedings: 3rd Symposium on Physiology and pathology of neuroglia; 2020 Noc 24-25; Virtual. Querétaro, México: Instituto de neurobiologia. 2020;:45.
https://hdl.handle.net/21.15107/rcub_rimsi_2017 .

Milicevic, Katarina, Bijelic, Dunja, Lazarevic, Milica, Miljkovic, Djordje, Bogdanović Pristov, Jelena, Petkovic, Branka, Andjus, Pavle, Momcilovic, Miljana, Nikolic, Ljiljana, "Central nervous system-infiltrated immune cells alter calcium dynamics in astrocytes" in Proceedings: 3rd Symposium on Physiology and pathology of neuroglia; 2020 Noc 24-25; Virtual. Querétaro, México: Instituto de neurobiologia (2020):45,
https://hdl.handle.net/21.15107/rcub_rimsi_2017 .

TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Bijelic, Dunja
AU  - Milicevic, Katarina
AU  - Momcilovic, Miljana
AU  - Bogdanović Pristov, Jelena
AU  - Petkovic, Branka
AU  - Andjus, Pavle
AU  - Miljkovic, Djordje
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2006
AB  - Aims: Multiple sclerosis (MS) is an in ammatory autoimmune disorder of the central nervous system (CNS). Complex interactions between inltrating immune cells (IIC) and resident glial cells of the CNS cause myelin loss and neuronal dysfunction in MS. Here we aim to understand how naïve astrocytes functionally respond to the IIC invasion of the CNS. 
Methods: We measured calcium activity of naïve astrocytes in culture upon application of IIC. An experimental autoimmune encephalomyelitis (EAE) MS rat model was used to isolate IIC from the spinal cord of animals at the symptomatic stage. Naïve astrocytes were isolated from the spinal cord of WT rats. 
Results: We show that IIC and not the lymph node immune cells evoke vigorous increase in the astrocyte calcium activity. This IIC-induced calcium response depends on an autocrine activation of the purinergic P2X7 receptors on the naïve astrocytes.We also show that IIC induce ATP release from astrocytes by a mechanism that involves gap junctions and/or hemichannels activation and not the vesicular pathway. Our data indicate that ATP release and subsequent increase in the astrocytic calcium activity mainly depends on the cell-cell contact between naïve astrocytes and IIC. 
Conclusions: These results show that naïve astrocytes functionally respond to the IIC by augmented release of ATP. An increase in ATP release would alter astrocyte-neuron communication and a ect neuronal function in MS.
PB  - Serbian Neuroscience Society, Belgrade
C3  - Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract
T1  - ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY
SP  - 295
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2006
ER  - 

@conference{
author = "Nikolic, Ljiljana and Bijelic, Dunja and Milicevic, Katarina and Momcilovic, Miljana and Bogdanović Pristov, Jelena and Petkovic, Branka and Andjus, Pavle and Miljkovic, Djordje",
year = "2019",
abstract = "Aims: Multiple sclerosis (MS) is an in ammatory autoimmune disorder of the central nervous system (CNS). Complex interactions between inltrating immune cells (IIC) and resident glial cells of the CNS cause myelin loss and neuronal dysfunction in MS. Here we aim to understand how naïve astrocytes functionally respond to the IIC invasion of the CNS. 
Methods: We measured calcium activity of naïve astrocytes in culture upon application of IIC. An experimental autoimmune encephalomyelitis (EAE) MS rat model was used to isolate IIC from the spinal cord of animals at the symptomatic stage. Naïve astrocytes were isolated from the spinal cord of WT rats. 
Results: We show that IIC and not the lymph node immune cells evoke vigorous increase in the astrocyte calcium activity. This IIC-induced calcium response depends on an autocrine activation of the purinergic P2X7 receptors on the naïve astrocytes.We also show that IIC induce ATP release from astrocytes by a mechanism that involves gap junctions and/or hemichannels activation and not the vesicular pathway. Our data indicate that ATP release and subsequent increase in the astrocytic calcium activity mainly depends on the cell-cell contact between naïve astrocytes and IIC. 
Conclusions: These results show that naïve astrocytes functionally respond to the IIC by augmented release of ATP. An increase in ATP release would alter astrocyte-neuron communication and a ect neuronal function in MS.",
publisher = "Serbian Neuroscience Society, Belgrade",
journal = "Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract",
title = "ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY",
pages = "295",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2006"
}

Nikolic, L., Bijelic, D., Milicevic, K., Momcilovic, M., Bogdanović Pristov, J., Petkovic, B., Andjus, P.,& Miljkovic, D.. (2019). ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY. in Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract
Serbian Neuroscience Society, Belgrade., 295.
https://hdl.handle.net/21.15107/rcub_rimsi_2006

Nikolic L, Bijelic D, Milicevic K, Momcilovic M, Bogdanović Pristov J, Petkovic B, Andjus P, Miljkovic D. ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY. in Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract. 2019;:295.
https://hdl.handle.net/21.15107/rcub_rimsi_2006 .

Nikolic, Ljiljana, Bijelic, Dunja, Milicevic, Katarina, Momcilovic, Miljana, Bogdanović Pristov, Jelena, Petkovic, Branka, Andjus, Pavle, Miljkovic, Djordje, "ASTROCYTE ACTIVITY IN THE CENTRAL NERVOUS SYSTEM AUTOIMMUNITY" in Federation of European Neuroscience Societies (FENS) Regional Meeting,Book of Abstract (2019):295,
https://hdl.handle.net/21.15107/rcub_rimsi_2006 .