TY  - CONF
AU  - Milosevic, Ana
AU  - Milosevic, Katarina
AU  - Nikolic, Ljiljana
AU  - Bogdanović Pristov, Jelena
AU  - Božić, Iva
AU  - Zivkovic, Anica
AU  - Lavrnja, Irena
AU  - Savic, Danijela
AU  - Janjic, Marija
AU  - Bjelobaba, Ivana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2019
AB  - Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide that controls 
mammalian reproduction by acting on its receptor (GnRHR) expressed on pituitary 
gonadotrope cells.  While GnRHR signaling in gonadotropes is well described, 
knowledge of GnRHR activation-related events at extrapituitary sites including 
neurons is limited. It was proposed that GnRH analogs (GnRHa) induce distinct 
changes in hippocampal gene expression, emotional processes, and cognitive 
functions.  
To explore neuronal GnRHR signaling we used the human neuroblastoma cell line 
SH-SY5Y. Further, we explored the regional expression of Gnrhr in rat brain and 
investigated the expression of several relevant genes in the hippocampus and preoptic 
area of peripubertal male rats treated with GnRHa. 
GNRHR is expressed in SH-SY5Y cell line, but its expression does not change after 
adding GnRHa in the incubation media. Electrophysiological recordings confirmed 
that GnRHa induced membrane depolarization but could not evoke action potentials. 
In the rat brain, Gnrhr expression could be detected in the hippocampus, amygdala, 
and hypothalamus, including the preoptic area. Prolonged treatment of peripubertal 
rats with GnRHa had no effect on the expression of genes in the hippocampus 
previously shown to be affected in the sheep model of delayed puberty.  
These results imply that neuronal GnRHR is either differently coupled (not coupled 
with Gq/11 protein), or that its membrane density is too low to induce transcriptional 
events. More investigation is needed to elucidate the role(s) of GnRH-GnRHR 
signaling in the brain.
PB  - Belgrade: Serbian Neuroscience Society
C3  - 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
T1  - GnRHR signaling in neuronal cells: in vitro and in vivo data
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2019
ER  - 

@conference{
author = "Milosevic, Ana and Milosevic, Katarina and Nikolic, Ljiljana and Bogdanović Pristov, Jelena and Božić, Iva and Zivkovic, Anica and Lavrnja, Irena and Savic, Danijela and Janjic, Marija and Bjelobaba, Ivana",
year = "2023",
abstract = "Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide that controls 
mammalian reproduction by acting on its receptor (GnRHR) expressed on pituitary 
gonadotrope cells.  While GnRHR signaling in gonadotropes is well described, 
knowledge of GnRHR activation-related events at extrapituitary sites including 
neurons is limited. It was proposed that GnRH analogs (GnRHa) induce distinct 
changes in hippocampal gene expression, emotional processes, and cognitive 
functions.  
To explore neuronal GnRHR signaling we used the human neuroblastoma cell line 
SH-SY5Y. Further, we explored the regional expression of Gnrhr in rat brain and 
investigated the expression of several relevant genes in the hippocampus and preoptic 
area of peripubertal male rats treated with GnRHa. 
GNRHR is expressed in SH-SY5Y cell line, but its expression does not change after 
adding GnRHa in the incubation media. Electrophysiological recordings confirmed 
that GnRHa induced membrane depolarization but could not evoke action potentials. 
In the rat brain, Gnrhr expression could be detected in the hippocampus, amygdala, 
and hypothalamus, including the preoptic area. Prolonged treatment of peripubertal 
rats with GnRHa had no effect on the expression of genes in the hippocampus 
previously shown to be affected in the sheep model of delayed puberty.  
These results imply that neuronal GnRHR is either differently coupled (not coupled 
with Gq/11 protein), or that its membrane density is too low to induce transcriptional 
events. More investigation is needed to elucidate the role(s) of GnRH-GnRHR 
signaling in the brain.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS",
title = "GnRHR signaling in neuronal cells: in vitro and in vivo data",
pages = "53",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2019"
}

Milosevic, A., Milosevic, K., Nikolic, L., Bogdanović Pristov, J., Božić, I., Zivkovic, A., Lavrnja, I., Savic, D., Janjic, M.,& Bjelobaba, I.. (2023). GnRHR signaling in neuronal cells: in vitro and in vivo data. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS
Belgrade: Serbian Neuroscience Society., 53.
https://hdl.handle.net/21.15107/rcub_rimsi_2019

Milosevic A, Milosevic K, Nikolic L, Bogdanović Pristov J, Božić I, Zivkovic A, Lavrnja I, Savic D, Janjic M, Bjelobaba I. GnRHR signaling in neuronal cells: in vitro and in vivo data. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS. 2023;:53.
https://hdl.handle.net/21.15107/rcub_rimsi_2019 .

Milosevic, Ana, Milosevic, Katarina, Nikolic, Ljiljana, Bogdanović Pristov, Jelena, Božić, Iva, Zivkovic, Anica, Lavrnja, Irena, Savic, Danijela, Janjic, Marija, Bjelobaba, Ivana, "GnRHR signaling in neuronal cells: in vitro and in vivo data" in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international  participation  31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS (2023):53,
https://hdl.handle.net/21.15107/rcub_rimsi_2019 .