TY  - CONF
AU  - Nikolic, Ljiljana
AU  - Vidović, Marija
AU  - Todorovic, Natasa
AU  - Petkovic, Branka
AU  - Stojadinovic, Gordana
AU  - Martac, Ljiljana
AU  - Bogdanović Pristov, Jelena
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2008
AB  - Investigating membrane properties of plants is a challenging task, considering that success of 
experiments is highly dependent on the possibility to isolate metabolically active protoplasts that 
can withstand membrane current recordings. The aim of the present work is to obtain viable 
protoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patch
clamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplasts 
with preserved membrane integrity suitable for electrophysiological experiments. We applied a 
custom approach for patch-clamping protoplasts using a microscope with a movable microscope 
stage. We recorded prominent inward and prominent outward types of membrane current profiles of protoplasts. Obtained data indicate that optimized isolation protocol and custom system for patch clamping, can be applied to study membrane properties of root protoplasts.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)
T1  - PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE
EP  - 274
SP  - 271
VL  - 1
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2008
ER  - 

@conference{
author = "Nikolic, Ljiljana and Vidović, Marija and Todorovic, Natasa and Petkovic, Branka and Stojadinovic, Gordana and Martac, Ljiljana and Bogdanović Pristov, Jelena",
year = "2022",
abstract = "Investigating membrane properties of plants is a challenging task, considering that success of 
experiments is highly dependent on the possibility to isolate metabolically active protoplasts that 
can withstand membrane current recordings. The aim of the present work is to obtain viable 
protoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patch
clamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplasts 
with preserved membrane integrity suitable for electrophysiological experiments. We applied a 
custom approach for patch-clamping protoplasts using a microscope with a movable microscope 
stage. We recorded prominent inward and prominent outward types of membrane current profiles of protoplasts. Obtained data indicate that optimized isolation protocol and custom system for patch clamping, can be applied to study membrane properties of root protoplasts.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)",
title = "PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE",
pages = "274-271",
volume = "1",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2008"
}

Nikolic, L., Vidović, M., Todorovic, N., Petkovic, B., Stojadinovic, G., Martac, L.,& Bogdanović Pristov, J.. (2022). PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings)
Society of Physical Chemists of Serbia., 1, 271-274.
https://hdl.handle.net/21.15107/rcub_rimsi_2008

Nikolic L, Vidović M, Todorovic N, Petkovic B, Stojadinovic G, Martac L, Bogdanović Pristov J. PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings). 2022;1:271-274.
https://hdl.handle.net/21.15107/rcub_rimsi_2008 .

Nikolic, Ljiljana, Vidović, Marija, Todorovic, Natasa, Petkovic, Branka, Stojadinovic, Gordana, Martac, Ljiljana, Bogdanović Pristov, Jelena, "PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE  WITH A MOVABLE STAGE" in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects  of Physical Chemistry (Proceedings), 1 (2022):271-274,
https://hdl.handle.net/21.15107/rcub_rimsi_2008 .