TY  - JOUR
AU  - Dević, M.
AU  - Momcilović, Ivana
AU  - Krstić, D.
AU  - Maksimović, Vuk
AU  - Konjević, R.
PY  - 2006
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/160
AB  - A protocol for in vitro propagation of Gentiana asclepiadea was developed. The best multiplication rate was obtained with woody plant medium (WPM) supplemented with 8.9 mu M BAP (6-benzylaminopurine) and 1.1 mu M IAA (indole-3-acetic acid). Gibberellic acid in the presence of 8.9 mu M BAP and 1.1 mu M IAA stimulated shoot elongation without affecting multiplication index. Although spontaneous rooting on hormone-free medium was observed, auxins increased the rooting ability. Treatment with IBA (indole-3-butyric acid) induced a higher number of roots, while the addition of IAA caused an increase in root length. The accumulation of mangiferin and gentiopicrine, in G. asclepiadea grown in vitro, was much lower in roots than in shoots. The accumulation of gentiopicrine and mangiferin in cultured plants was significantly enhanced in the presence of cytokinins (BAP), even above the level of plants from natural habitat.
T2  - Phyton - Annales Rei Botanicae
T1  - In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin
EP  - 54
IS  - 1
SP  - 45
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_160
ER  - 

@article{
author = "Dević, M. and Momcilović, Ivana and Krstić, D. and Maksimović, Vuk and Konjević, R.",
year = "2006",
abstract = "A protocol for in vitro propagation of Gentiana asclepiadea was developed. The best multiplication rate was obtained with woody plant medium (WPM) supplemented with 8.9 mu M BAP (6-benzylaminopurine) and 1.1 mu M IAA (indole-3-acetic acid). Gibberellic acid in the presence of 8.9 mu M BAP and 1.1 mu M IAA stimulated shoot elongation without affecting multiplication index. Although spontaneous rooting on hormone-free medium was observed, auxins increased the rooting ability. Treatment with IBA (indole-3-butyric acid) induced a higher number of roots, while the addition of IAA caused an increase in root length. The accumulation of mangiferin and gentiopicrine, in G. asclepiadea grown in vitro, was much lower in roots than in shoots. The accumulation of gentiopicrine and mangiferin in cultured plants was significantly enhanced in the presence of cytokinins (BAP), even above the level of plants from natural habitat.",
journal = "Phyton - Annales Rei Botanicae",
title = "In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin",
pages = "54-45",
number = "1",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_160"
}

Dević, M., Momcilović, I., Krstić, D., Maksimović, V.,& Konjević, R.. (2006). In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin. in Phyton - Annales Rei Botanicae, 46(1), 45-54.
https://hdl.handle.net/21.15107/rcub_rimsi_160

Dević M, Momcilović I, Krstić D, Maksimović V, Konjević R. In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin. in Phyton - Annales Rei Botanicae. 2006;46(1):45-54.
https://hdl.handle.net/21.15107/rcub_rimsi_160 .

Dević, M., Momcilović, Ivana, Krstić, D., Maksimović, Vuk, Konjević, R., "In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin" in Phyton - Annales Rei Botanicae, 46, no. 1 (2006):45-54,
https://hdl.handle.net/21.15107/rcub_rimsi_160 .

TY  - JOUR
AU  - Misic, Danijela
AU  - Maksimović, Vuk
AU  - Todorović, S
AU  - Grubisic, D
AU  - Konjević, R
PY  - 2005
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/132
AB  - A wide range of sucrose, fructose, and glucose levels in culture media was tested in vitro in order to determine those that provide optimum growth, morphogenesis, and the production of secondary metabolites in Nepeta rtanjensis Diklic and Milojevic shoots. The effect of different concentrations of the carbohydrates in culture media on the internal carbohydrate status of N. rtanjensis shoots was also determined. Our results show that in vitro growth and development of N. rtanjensis, as well as nepetalactone accumulation, are significantly affected by both the type of carbohydrate and its concentration in the culture medium. Glucose proved to be the most efficient carbon and energy source.
PB  - Brill Academic Publishers, Leiden
T2  - Israel Journal of Plant Sciences
T1  - Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro
EP  - 108
IS  - 2
SP  - 103
VL  - 53
DO  - 10.1560/CBU6-G71U-TC65-FH1U
ER  - 

@article{
author = "Misic, Danijela and Maksimović, Vuk and Todorović, S and Grubisic, D and Konjević, R",
year = "2005",
abstract = "A wide range of sucrose, fructose, and glucose levels in culture media was tested in vitro in order to determine those that provide optimum growth, morphogenesis, and the production of secondary metabolites in Nepeta rtanjensis Diklic and Milojevic shoots. The effect of different concentrations of the carbohydrates in culture media on the internal carbohydrate status of N. rtanjensis shoots was also determined. Our results show that in vitro growth and development of N. rtanjensis, as well as nepetalactone accumulation, are significantly affected by both the type of carbohydrate and its concentration in the culture medium. Glucose proved to be the most efficient carbon and energy source.",
publisher = "Brill Academic Publishers, Leiden",
journal = "Israel Journal of Plant Sciences",
title = "Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro",
pages = "108-103",
number = "2",
volume = "53",
doi = "10.1560/CBU6-G71U-TC65-FH1U"
}

Misic, D., Maksimović, V., Todorović, S., Grubisic, D.,& Konjević, R.. (2005). Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro. in Israel Journal of Plant Sciences
Brill Academic Publishers, Leiden., 53(2), 103-108.
https://doi.org/10.1560/CBU6-G71U-TC65-FH1U

Misic D, Maksimović V, Todorović S, Grubisic D, Konjević R. Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro. in Israel Journal of Plant Sciences. 2005;53(2):103-108.
doi:10.1560/CBU6-G71U-TC65-FH1U .

Misic, Danijela, Maksimović, Vuk, Todorović, S, Grubisic, D, Konjević, R, "Influence of carbohydrate source on Nepeta rtanjensis growth, morphogenesis, and nepetalactone production in vitro" in Israel Journal of Plant Sciences, 53, no. 2 (2005):103-108,
https://doi.org/10.1560/CBU6-G71U-TC65-FH1U . .