TY  - JOUR
AU  - Misic, Danijela
AU  - Dragicević, Milan
AU  - Siler, Branislav
AU  - Nestorović-Živković, Jasmina M
AU  - Maksimović, Vuk
AU  - Momcilović, Ivana
AU  - Nikolic, Miroslav
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/542
AB  - A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress
EP  - 1289
IS  - 13
SP  - 1281
VL  - 169
DO  - 10.1016/j.jplph.2012.04.018
ER  - 

@article{
author = "Misic, Danijela and Dragicević, Milan and Siler, Branislav and Nestorović-Živković, Jasmina M and Maksimović, Vuk and Momcilović, Ivana and Nikolic, Miroslav",
year = "2012",
abstract = "A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress",
pages = "1289-1281",
number = "13",
volume = "169",
doi = "10.1016/j.jplph.2012.04.018"
}

Misic, D., Dragicević, M., Siler, B., Nestorović-Živković, J. M., Maksimović, V., Momcilović, I.,& Nikolic, M.. (2012). Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 169(13), 1281-1289.
https://doi.org/10.1016/j.jplph.2012.04.018

Misic D, Dragicević M, Siler B, Nestorović-Živković JM, Maksimović V, Momcilović I, Nikolic M. Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology. 2012;169(13):1281-1289.
doi:10.1016/j.jplph.2012.04.018 .

Misic, Danijela, Dragicević, Milan, Siler, Branislav, Nestorović-Živković, Jasmina M, Maksimović, Vuk, Momcilović, Ivana, Nikolic, Miroslav, "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress" in Journal of Plant Physiology, 169, no. 13 (2012):1281-1289,
https://doi.org/10.1016/j.jplph.2012.04.018 . .

TY  - JOUR
AU  - Živković, Suzana
AU  - Popović, M
AU  - Dragišić Maksimović, Jelena
AU  - Momcilović, Ivana
AU  - Grubisic, Dragoljub V
PY  - 2010
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/377
AB  - Asplenium ceterach belongs to a group of poikilohydric ferns and it can recover uninjured from an almost completely dehydrated stale. In our study, short term dehydration (24h) at four different water potentials, resulted in moderate water loss (partial desiccation) in fern tissue. The main phenolic acids represented in A. ceterach were chlorogenic (CGA) and caffeic acid (CA) and their content decreased during the dehydration process. For the first time, peroxidase (POD) and polyphenol oxidase (PPO) isoforms were determined in the rustyback fern. The results exhibit the presence of numerous anionic POD isoforms, with pI ranging from 4.4 to 5.8, but none of the cationic isoforms was detected. Two PPO isofoms were identified, one anionic with pI 6.3 and one cationic with pI of about 9.0. Short-term dehydration brought about a remarkable increase in POD and PPO activity using CGA as a substrate. Changes in enzyme activity and content of substrates during dehydration may play an important role in the adaptation of the rustyback fern to water deficit, and increase the overall plant resistance to stress conditions.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l.
EP  - 1081
IS  - 4
SP  - 1071
VL  - 62
DO  - 10.2298/ABS1004071Z
ER  - 

@article{
author = "Živković, Suzana and Popović, M and Dragišić Maksimović, Jelena and Momcilović, Ivana and Grubisic, Dragoljub V",
year = "2010",
abstract = "Asplenium ceterach belongs to a group of poikilohydric ferns and it can recover uninjured from an almost completely dehydrated stale. In our study, short term dehydration (24h) at four different water potentials, resulted in moderate water loss (partial desiccation) in fern tissue. The main phenolic acids represented in A. ceterach were chlorogenic (CGA) and caffeic acid (CA) and their content decreased during the dehydration process. For the first time, peroxidase (POD) and polyphenol oxidase (PPO) isoforms were determined in the rustyback fern. The results exhibit the presence of numerous anionic POD isoforms, with pI ranging from 4.4 to 5.8, but none of the cationic isoforms was detected. Two PPO isofoms were identified, one anionic with pI 6.3 and one cationic with pI of about 9.0. Short-term dehydration brought about a remarkable increase in POD and PPO activity using CGA as a substrate. Changes in enzyme activity and content of substrates during dehydration may play an important role in the adaptation of the rustyback fern to water deficit, and increase the overall plant resistance to stress conditions.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l.",
pages = "1081-1071",
number = "4",
volume = "62",
doi = "10.2298/ABS1004071Z"
}

Živković, S., Popović, M., Dragišić Maksimović, J., Momcilović, I.,& Grubisic, D. V.. (2010). Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l.. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 62(4), 1071-1081.
https://doi.org/10.2298/ABS1004071Z

Živković S, Popović M, Dragišić Maksimović J, Momcilović I, Grubisic DV. Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l.. in Archives of Biological Sciences. 2010;62(4):1071-1081.
doi:10.2298/ABS1004071Z .

Živković, Suzana, Popović, M, Dragišić Maksimović, Jelena, Momcilović, Ivana, Grubisic, Dragoljub V, "Dehydration-related changes of peroxidase and polyphenol oxidase activity in fronds of the resurrection fern asplenium ceterach l." in Archives of Biological Sciences, 62, no. 4 (2010):1071-1081,
https://doi.org/10.2298/ABS1004071Z . .

TY  - JOUR
AU  - Dević, M.
AU  - Momcilović, Ivana
AU  - Krstić, D.
AU  - Maksimović, Vuk
AU  - Konjević, R.
PY  - 2006
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/160
AB  - A protocol for in vitro propagation of Gentiana asclepiadea was developed. The best multiplication rate was obtained with woody plant medium (WPM) supplemented with 8.9 mu M BAP (6-benzylaminopurine) and 1.1 mu M IAA (indole-3-acetic acid). Gibberellic acid in the presence of 8.9 mu M BAP and 1.1 mu M IAA stimulated shoot elongation without affecting multiplication index. Although spontaneous rooting on hormone-free medium was observed, auxins increased the rooting ability. Treatment with IBA (indole-3-butyric acid) induced a higher number of roots, while the addition of IAA caused an increase in root length. The accumulation of mangiferin and gentiopicrine, in G. asclepiadea grown in vitro, was much lower in roots than in shoots. The accumulation of gentiopicrine and mangiferin in cultured plants was significantly enhanced in the presence of cytokinins (BAP), even above the level of plants from natural habitat.
T2  - Phyton - Annales Rei Botanicae
T1  - In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin
EP  - 54
IS  - 1
SP  - 45
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_160
ER  - 

@article{
author = "Dević, M. and Momcilović, Ivana and Krstić, D. and Maksimović, Vuk and Konjević, R.",
year = "2006",
abstract = "A protocol for in vitro propagation of Gentiana asclepiadea was developed. The best multiplication rate was obtained with woody plant medium (WPM) supplemented with 8.9 mu M BAP (6-benzylaminopurine) and 1.1 mu M IAA (indole-3-acetic acid). Gibberellic acid in the presence of 8.9 mu M BAP and 1.1 mu M IAA stimulated shoot elongation without affecting multiplication index. Although spontaneous rooting on hormone-free medium was observed, auxins increased the rooting ability. Treatment with IBA (indole-3-butyric acid) induced a higher number of roots, while the addition of IAA caused an increase in root length. The accumulation of mangiferin and gentiopicrine, in G. asclepiadea grown in vitro, was much lower in roots than in shoots. The accumulation of gentiopicrine and mangiferin in cultured plants was significantly enhanced in the presence of cytokinins (BAP), even above the level of plants from natural habitat.",
journal = "Phyton - Annales Rei Botanicae",
title = "In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin",
pages = "54-45",
number = "1",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_160"
}

Dević, M., Momcilović, I., Krstić, D., Maksimović, V.,& Konjević, R.. (2006). In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin. in Phyton - Annales Rei Botanicae, 46(1), 45-54.
https://hdl.handle.net/21.15107/rcub_rimsi_160

Dević M, Momcilović I, Krstić D, Maksimović V, Konjević R. In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin. in Phyton - Annales Rei Botanicae. 2006;46(1):45-54.
https://hdl.handle.net/21.15107/rcub_rimsi_160 .

Dević, M., Momcilović, Ivana, Krstić, D., Maksimović, Vuk, Konjević, R., "In vitro multiplication of willow gentian (Gentiana asclepiadea L.) and the production of gentiopicrine and mangiferin" in Phyton - Annales Rei Botanicae, 46, no. 1 (2006):45-54,
https://hdl.handle.net/21.15107/rcub_rimsi_160 .