TY  - CONF
AU  - Prokopijević, Miloš
AU  - Prodanović, Olivera
AU  - Spasojević, Dragica
AU  - Stanković, Mira
AU  - Stojanović, Željko
AU  - Radotić, Ksenija
AU  - Prodanović, Radivoje
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1806
AB  - Toxic aromatic pollutants that are found in various industrial wastewaters pose a serious environmental threat. Current methods for phenol removal have certain disadvantages, such as low efficiency, high cost or generation of even more toxic products. On the other hand enzyme-based treatments are highly selective and efficient. Soybean hull peroxidase (SHP) as well as other class III peroxidases catalyzes oxidation reaction in the presence of hydrogen peroxide, resulting in phenol polymerization and formation of less hazardous
phenolic polymers. As a by-product of the food industry, soybean hulls are inexpensive and readily available source of large quantities of crude peroxidase. The aim of our research was to isolate SHP from soybean
hulls and immobilize it onto a glycidyl methacrylate based carriers using glutaraldehyde method and characterize the resulting product. Immobilized SHP showed dependence upon the pore size of the carrier matrix,with the highest obtained specific activity of 22.8 U g-1 of carrier. Immobilized enzyme proved as an effective phenol removal alternative method with improved thermal and organic solvent stabilities compared to the free form. It also showed greater stability and tolerance to pH fluctuations, showing higher specific activities over a wider pH range. Operational stability was tested by repeated pyrogallol oxidation cycles in a batch reactor. After three cycles, immobilized SHP retained over 60% of the initial activity.
PB  - Serbian Plant Physiology Society, Institute for Biological Research „Siniša Stanković“, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia
C3  - 2st International Conference on Plant Biology • 21th Symposium of the Serbian Plant Physiology Society
T1  - Characterization of soybean hull peroxidase immobilized on glycidyl methacrylate copolymers
EP  - 18
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1806
ER  - 

@conference{
author = "Prokopijević, Miloš and Prodanović, Olivera and Spasojević, Dragica and Stanković, Mira and Stojanović, Željko and Radotić, Ksenija and Prodanović, Radivoje",
year = "2015",
abstract = "Toxic aromatic pollutants that are found in various industrial wastewaters pose a serious environmental threat. Current methods for phenol removal have certain disadvantages, such as low efficiency, high cost or generation of even more toxic products. On the other hand enzyme-based treatments are highly selective and efficient. Soybean hull peroxidase (SHP) as well as other class III peroxidases catalyzes oxidation reaction in the presence of hydrogen peroxide, resulting in phenol polymerization and formation of less hazardous
phenolic polymers. As a by-product of the food industry, soybean hulls are inexpensive and readily available source of large quantities of crude peroxidase. The aim of our research was to isolate SHP from soybean
hulls and immobilize it onto a glycidyl methacrylate based carriers using glutaraldehyde method and characterize the resulting product. Immobilized SHP showed dependence upon the pore size of the carrier matrix,with the highest obtained specific activity of 22.8 U g-1 of carrier. Immobilized enzyme proved as an effective phenol removal alternative method with improved thermal and organic solvent stabilities compared to the free form. It also showed greater stability and tolerance to pH fluctuations, showing higher specific activities over a wider pH range. Operational stability was tested by repeated pyrogallol oxidation cycles in a batch reactor. After three cycles, immobilized SHP retained over 60% of the initial activity.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research „Siniša Stanković“, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia",
journal = "2st International Conference on Plant Biology • 21th Symposium of the Serbian Plant Physiology Society",
title = "Characterization of soybean hull peroxidase immobilized on glycidyl methacrylate copolymers",
pages = "18-17",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1806"
}

Prokopijević, M., Prodanović, O., Spasojević, D., Stanković, M., Stojanović, Ž., Radotić, K.,& Prodanović, R.. (2015). Characterization of soybean hull peroxidase immobilized on glycidyl methacrylate copolymers. in 2st International Conference on Plant Biology • 21th Symposium of the Serbian Plant Physiology Society
Serbian Plant Physiology Society, Institute for Biological Research „Siniša Stanković“, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia., 17-18.
https://hdl.handle.net/21.15107/rcub_rimsi_1806

Prokopijević M, Prodanović O, Spasojević D, Stanković M, Stojanović Ž, Radotić K, Prodanović R. Characterization of soybean hull peroxidase immobilized on glycidyl methacrylate copolymers. in 2st International Conference on Plant Biology • 21th Symposium of the Serbian Plant Physiology Society. 2015;:17-18.
https://hdl.handle.net/21.15107/rcub_rimsi_1806 .

Prokopijević, Miloš, Prodanović, Olivera, Spasojević, Dragica, Stanković, Mira, Stojanović, Željko, Radotić, Ksenija, Prodanović, Radivoje, "Characterization of soybean hull peroxidase immobilized on glycidyl methacrylate copolymers" in 2st International Conference on Plant Biology • 21th Symposium of the Serbian Plant Physiology Society (2015):17-18,
https://hdl.handle.net/21.15107/rcub_rimsi_1806 .

TY  - CONF
AU  - Prodanović, Olivera
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Stojanović, Željko
AU  - Radotić, Ksenija
AU  - Knežević-Jugović, Zorica
AU  - Prodanović, Radivoje
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3126
AB  - U ovom radu peroksidaza iz rena (HRP) je kovalentno imobilizovana na kopolimeru glicidil metakrilata i
etilenglikol dimetakrilata pomoću dve kovalentne metode. Ispitani su uticaji prečnika pora i površinskih
karakteristika kopolimera na enzimsku aktivnost i stabilnost. Enzim imobilizovan perjodatnom metodom je
pokazao veću aktivnost i stabilnost u poređenju sa glutaraldehidnom metodom. HRP imobilizovana na
kopolimer SGE-20/12 sa prečnikom pora od 120 nm je pokazala najveću specifičnu aktivnost i dobru
termostabilnost. Km vrednost imobilizata je bila 10,8 mM što je oko 5,6 puta više od rastvornog enzima. Obe
forme enzima su pokazale optimum na pH 7, ali je imobilizovana HRP pokazala širi pH optimum u odnosu na
rastvornu formu. Operaciona stabilnost je bila zadovoljavajuća i nakon pet uzastopnih upotreba zaostala
aktivnost je bila 45%.
PB  - Srpsko hemijsko društvo
C3  - 50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15.juni 2012.
T1  - Covalent immobilization of horseradish peroxidase on macroporous glycidyl methacrylate based copolymer
EP  - 198
SP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3126
ER  - 

@conference{
author = "Prodanović, Olivera and Prokopijević, Miloš and Spasojević, Dragica and Stojanović, Željko and Radotić, Ksenija and Knežević-Jugović, Zorica and Prodanović, Radivoje",
year = "2012",
abstract = "U ovom radu peroksidaza iz rena (HRP) je kovalentno imobilizovana na kopolimeru glicidil metakrilata i
etilenglikol dimetakrilata pomoću dve kovalentne metode. Ispitani su uticaji prečnika pora i površinskih
karakteristika kopolimera na enzimsku aktivnost i stabilnost. Enzim imobilizovan perjodatnom metodom je
pokazao veću aktivnost i stabilnost u poređenju sa glutaraldehidnom metodom. HRP imobilizovana na
kopolimer SGE-20/12 sa prečnikom pora od 120 nm je pokazala najveću specifičnu aktivnost i dobru
termostabilnost. Km vrednost imobilizata je bila 10,8 mM što je oko 5,6 puta više od rastvornog enzima. Obe
forme enzima su pokazale optimum na pH 7, ali je imobilizovana HRP pokazala širi pH optimum u odnosu na
rastvornu formu. Operaciona stabilnost je bila zadovoljavajuća i nakon pet uzastopnih upotreba zaostala
aktivnost je bila 45%.",
publisher = "Srpsko hemijsko društvo",
journal = "50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15.juni 2012.",
title = "Covalent immobilization of horseradish peroxidase on macroporous glycidyl methacrylate based copolymer",
pages = "198-195",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3126"
}

Prodanović, O., Prokopijević, M., Spasojević, D., Stojanović, Ž., Radotić, K., Knežević-Jugović, Z.,& Prodanović, R.. (2012). Covalent immobilization of horseradish peroxidase on macroporous glycidyl methacrylate based copolymer. in 50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15.juni 2012.
Srpsko hemijsko društvo., 195-198.
https://hdl.handle.net/21.15107/rcub_rimsi_3126

Prodanović O, Prokopijević M, Spasojević D, Stojanović Ž, Radotić K, Knežević-Jugović Z, Prodanović R. Covalent immobilization of horseradish peroxidase on macroporous glycidyl methacrylate based copolymer. in 50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15.juni 2012.. 2012;:195-198.
https://hdl.handle.net/21.15107/rcub_rimsi_3126 .

Prodanović, Olivera, Prokopijević, Miloš, Spasojević, Dragica, Stojanović, Željko, Radotić, Ksenija, Knežević-Jugović, Zorica, Prodanović, Radivoje, "Covalent immobilization of horseradish peroxidase on macroporous glycidyl methacrylate based copolymer" in 50. Savetovanje Srpskog hemijskog društva, Beograd, 14. i 15.juni 2012. (2012):195-198,
https://hdl.handle.net/21.15107/rcub_rimsi_3126 .