TY  - CONF
AU  - Stanišić, Marija
AU  - Popović, Nikolina
AU  - Prodanović, Olivera
AU  - Prodanović, Radivoje
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2912
AB  - Pectins belong to a group of a plant polysaccharides that are structural components of plant
cell walls. These polysaccharides have big potential for use in the food industry as a
gelling agents and biomedical application due their biocompatibility, biodegradability, low
price, etc. Chemical modifications of pectin are useful methods for introducing various
functional groups, which give pectin hydrogels novel properties. In this study, pectin was
modified by oxidation with sodium periodate at molar ratios of 5, 10 and 15 mol% and
reductive amination with dopamine and sodium cyanoborohydride afterwards. This
modification of pectin was confirmed by UV-VIS and NMR spectroscopy. Dopaminepectins showed gelling properties in the presence of laccase and oxygen. These pectin
derivatives were tested as carriers for laccase immobilization within microbeads formed in
an emulsion based enzymatic polymerization reaction. Laccase was expressed in functional
form in E. coli, isolated and additionally purified. We determined optimal conditions for
immobilization andobtained microbeads with specific activity of 0.0006 IU/mg and 40%
yield of immobilization. The immobilized laccase could be used for various application,
such as decolorization of textile dyes 
.
PB  - Serbian Biochemical Society
C3  - The 9th conference of the Serbian Biochemical Society: Diversity in Biochemistry
T1  - Dopamine-modified pectins for laccase induced hydrogel formation and immobilization
EP  - 168
SP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2912
ER  - 

@conference{
author = "Stanišić, Marija and Popović, Nikolina and Prodanović, Olivera and Prodanović, Radivoje",
year = "2019",
abstract = "Pectins belong to a group of a plant polysaccharides that are structural components of plant
cell walls. These polysaccharides have big potential for use in the food industry as a
gelling agents and biomedical application due their biocompatibility, biodegradability, low
price, etc. Chemical modifications of pectin are useful methods for introducing various
functional groups, which give pectin hydrogels novel properties. In this study, pectin was
modified by oxidation with sodium periodate at molar ratios of 5, 10 and 15 mol% and
reductive amination with dopamine and sodium cyanoborohydride afterwards. This
modification of pectin was confirmed by UV-VIS and NMR spectroscopy. Dopaminepectins showed gelling properties in the presence of laccase and oxygen. These pectin
derivatives were tested as carriers for laccase immobilization within microbeads formed in
an emulsion based enzymatic polymerization reaction. Laccase was expressed in functional
form in E. coli, isolated and additionally purified. We determined optimal conditions for
immobilization andobtained microbeads with specific activity of 0.0006 IU/mg and 40%
yield of immobilization. The immobilized laccase could be used for various application,
such as decolorization of textile dyes 
.",
publisher = "Serbian Biochemical Society",
journal = "The 9th conference of the Serbian Biochemical Society: Diversity in Biochemistry",
title = "Dopamine-modified pectins for laccase induced hydrogel formation and immobilization",
pages = "168-168",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2912"
}

Stanišić, M., Popović, N., Prodanović, O.,& Prodanović, R.. (2019). Dopamine-modified pectins for laccase induced hydrogel formation and immobilization. in The 9th conference of the Serbian Biochemical Society: Diversity in Biochemistry
Serbian Biochemical Society., 168-168.
https://hdl.handle.net/21.15107/rcub_rimsi_2912

Stanišić M, Popović N, Prodanović O, Prodanović R. Dopamine-modified pectins for laccase induced hydrogel formation and immobilization. in The 9th conference of the Serbian Biochemical Society: Diversity in Biochemistry. 2019;:168-168.
https://hdl.handle.net/21.15107/rcub_rimsi_2912 .

Stanišić, Marija, Popović, Nikolina, Prodanović, Olivera, Prodanović, Radivoje, "Dopamine-modified pectins for laccase induced hydrogel formation and immobilization" in The 9th conference of the Serbian Biochemical Society: Diversity in Biochemistry (2019):168-168,
https://hdl.handle.net/21.15107/rcub_rimsi_2912 .