TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragicević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Perisic, Mirjana
AU  - Misic, Danijela
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1398
AB  - Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days exposure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Antagonistic Interaction between Phosphinothricin and Nepeta rtanjensis Essential Oil Affected Ammonium Metabolism and Antioxidant Defense of Arabidopsis Grown In Vitro
IS  - 1
VL  - 10
DO  - 10.3390/plants10010142
ER  - 

@article{
author = "Dmitrović, Slavica and Dragicević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Perisic, Mirjana and Misic, Danijela",
year = "2021",
abstract = "Phosphinothricin (PPT) is one of the most widely used herbicides. PTT targets glutamine synthetase (GS) activity in plants, and its phytotoxicity is ascribed to ammonium accumulation and reactive oxygen species bursts, which drives rapid lipid peroxidation of cell membranes. In agricultural fields, PPT is extensively sprayed on plant foliage; however, a portion of the herbicide reaches the soil. According to the present study, PPT absorbed via roots can be phytotoxic to Arabidopsis, inducing more adverse effects in roots than in shoots. Alterations in plant physiology caused by 10 days exposure to herbicide via roots are reflected through growth suppression, reduced chlorophyll content, perturbations in the sugar and organic acid metabolism, modifications in the activities and abundances of GS, catalase, peroxidase, and superoxide dismutase. Antagonistic interaction of Nepeta rtanjensis essential oil (NrEO) and PPT, emphasizes the existence of complex control mechanisms at the transcriptional and posttranslational level, which result in the mitigation of PPT-induced ammonium toxicity and in providing more efficient antioxidant defense of plants. Simultaneous application of the two agents in the field cannot be recommended; however, NrEO might be considered as the PPT post-treatment for reducing harmful effects of herbicide residues in the soil on non-target plants.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Antagonistic Interaction between Phosphinothricin and Nepeta rtanjensis Essential Oil Affected Ammonium Metabolism and Antioxidant Defense of Arabidopsis Grown In Vitro",
number = "1",
volume = "10",
doi = "10.3390/plants10010142"
}

Dmitrović, S., Dragicević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D., Perisic, M.,& Misic, D.. (2021). Antagonistic Interaction between Phosphinothricin and Nepeta rtanjensis Essential Oil Affected Ammonium Metabolism and Antioxidant Defense of Arabidopsis Grown In Vitro. in Plants-Basel
MDPI, Basel., 10(1).
https://doi.org/10.3390/plants10010142

Dmitrović S, Dragicević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Perisic M, Misic D. Antagonistic Interaction between Phosphinothricin and Nepeta rtanjensis Essential Oil Affected Ammonium Metabolism and Antioxidant Defense of Arabidopsis Grown In Vitro. in Plants-Basel. 2021;10(1).
doi:10.3390/plants10010142 .

Dmitrović, Slavica, Dragicević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Perisic, Mirjana, Misic, Danijela, "Antagonistic Interaction between Phosphinothricin and Nepeta rtanjensis Essential Oil Affected Ammonium Metabolism and Antioxidant Defense of Arabidopsis Grown In Vitro" in Plants-Basel, 10, no. 1 (2021),
https://doi.org/10.3390/plants10010142 . .

TY  - JOUR
AU  - Živković, Suzana
AU  - Milutinović, Milica
AU  - Maksimović, Vuk
AU  - Ciric, Ana
AU  - Ivanov, Marija
AU  - Bozunović, Jelena
AU  - Banjanac, Tijana
AU  - Misic, Danijela
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1368
AB  - Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH center dot and ABTS(center dot+)), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(-)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.
PB  - Elsevier, Amsterdam
T2  - South African Journal of Botany
T1  - Antioxidant and antimicrobial activity of two Asplenium species
EP  - 187
SP  - 180
VL  - 132
DO  - 10.1016/j.sajb.2020.03.034
ER  - 

@article{
author = "Živković, Suzana and Milutinović, Milica and Maksimović, Vuk and Ciric, Ana and Ivanov, Marija and Bozunović, Jelena and Banjanac, Tijana and Misic, Danijela",
year = "2020",
abstract = "Extracts of the aerial (fronds) and underground parts (rhizome and roots) of Asplenium adiantum-nigrum L. and Asplenium ruta-muraria L. were analyzed for their phenolic composition and antioxidant properties (scavenging capacities against DPPH center dot and ABTS(center dot+)), and were comparatively assayed for antimicrobial and antibiofilm activity. Total of the 17 phenolic compounds, belonging to the classes of phenolic acids (hydroxybenzoic and hydroxycinnamic acids), flavonoids (flavan-3-ols, flavonols), and xanthones were identified and quantified using UHPLC/DAD/(-)HESI-MS/MS analysis. The present study shows that two analyzed ferns are significantly diverse in terms of their phenolic profile. Flavonoids considerably contribute to the total phenolic content of A. ruta-muraria, ranging from 47% in roots to around 75% in rhizome and fronds, while A. adiantum-nigrum preferably accumulates xanthones. Antioxidant activity of fern methanol extracts was ascribed to their phenolics content. Extracts of rhizomes of both Asplenium ferns exhibited the highest antimicrobial activity, while frond extracts were more efficient in inhibition of Candida albicans biofilm formation. The present study provides experimental evidence that methanol extracts from two analyzed Asplenium fern species exhibit strong antibacterial effect against Gram-negative bacteria which candidate them as a potentially useful natural antimicrobial agents.",
publisher = "Elsevier, Amsterdam",
journal = "South African Journal of Botany",
title = "Antioxidant and antimicrobial activity of two Asplenium species",
pages = "187-180",
volume = "132",
doi = "10.1016/j.sajb.2020.03.034"
}

Živković, S., Milutinović, M., Maksimović, V., Ciric, A., Ivanov, M., Bozunović, J., Banjanac, T.,& Misic, D.. (2020). Antioxidant and antimicrobial activity of two Asplenium species. in South African Journal of Botany
Elsevier, Amsterdam., 132, 180-187.
https://doi.org/10.1016/j.sajb.2020.03.034

Živković S, Milutinović M, Maksimović V, Ciric A, Ivanov M, Bozunović J, Banjanac T, Misic D. Antioxidant and antimicrobial activity of two Asplenium species. in South African Journal of Botany. 2020;132:180-187.
doi:10.1016/j.sajb.2020.03.034 .

Živković, Suzana, Milutinović, Milica, Maksimović, Vuk, Ciric, Ana, Ivanov, Marija, Bozunović, Jelena, Banjanac, Tijana, Misic, Danijela, "Antioxidant and antimicrobial activity of two Asplenium species" in South African Journal of Botany, 132 (2020):180-187,
https://doi.org/10.1016/j.sajb.2020.03.034 . .

TY  - JOUR
AU  - Dmitrović, Slavica
AU  - Dragicević, Milan
AU  - Savić, Jelena
AU  - Milutinović, Milica
AU  - Živković, Suzana
AU  - Maksimović, Vuk
AU  - Matekalo, Dragana
AU  - Misic, Danijela
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1244
AB  - Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.
EP  - 94
SP  - 87
VL  - 237
DO  - 10.1016/j.jplph.2019.04.006
ER  - 

@article{
author = "Dmitrović, Slavica and Dragicević, Milan and Savić, Jelena and Milutinović, Milica and Živković, Suzana and Maksimović, Vuk and Matekalo, Dragana and Misic, Danijela",
year = "2019",
abstract = "Active ingredient of the commercial herbicide BASTA (B), phosphinothricin, acts as an inhibitor of glutamine synthetase (GS), a key enzyme in ammonium assimilation. The treatment with BASTA leads to an elevation of ammonium levels in plants and further to various physiological alterations, ammonium toxicity and lethality. Results of the present study emphasize the complexity underlying control mechanisms that determine BASTA interaction with essential oil (EO) from Nepeta rtanjensis (NrEO), bioherbicide inducing oxidative stress in target plants. Simultaneous application of NrEO and BASTA, two agents showing differential mode of action, suspends BASTA-induced ammonium toxicity in Arabidopsis thaliana plants. This is achieved through maintaining GS activity, which sustains a sub-toxic and/or sub-lethal ammonium concentration in tissues. As revealed by the present study, regulation of GS activity, as influenced by BASTA and NrEO, occurs at transcriptional, posttranscriptional, and/or posttranslational levels. Two genes encoding cytosolic GS, GLN1;1 and GLN1;3, are highlighted as the main isozymes in Arabidopsis shoots contributing to NrEO-induced overcoming of BASTA-generated ammonium toxicity. The effects of NrEO might be ascribed to its major component nepetalactone, but the contribution of minor EO components should not be neglected. Although of fundamental significance, the results of the present study suggest possible low efficiency of BASTA in plantations of medicinal/aromatic plants such as Nepeta species. Furthermore, these results highlight the possibility of using NrEO as a bioherbicide in BASTA-treated crop fields to mitigate the effect of BASTA residues in contaminated soils.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.",
pages = "94-87",
volume = "237",
doi = "10.1016/j.jplph.2019.04.006"
}

Dmitrović, S., Dragicević, M., Savić, J., Milutinović, M., Živković, S., Maksimović, V., Matekalo, D.,& Misic, D.. (2019). Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 237, 87-94.
https://doi.org/10.1016/j.jplph.2019.04.006

Dmitrović S, Dragicević M, Savić J, Milutinović M, Živković S, Maksimović V, Matekalo D, Misic D. Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh.. in Journal of Plant Physiology. 2019;237:87-94.
doi:10.1016/j.jplph.2019.04.006 .

Dmitrović, Slavica, Dragicević, Milan, Savić, Jelena, Milutinović, Milica, Živković, Suzana, Maksimović, Vuk, Matekalo, Dragana, Misic, Danijela, "Nepetalactone-rich essential oil mitigates phosphinothricin-induced ammonium toxicity in Arabidopsis thaliana (L.) Heynh." in Journal of Plant Physiology, 237 (2019):87-94,
https://doi.org/10.1016/j.jplph.2019.04.006 . .