TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragicević, Milan
AU  - Tešević, Vele
AU  - Stankovic-Jeremic, Jovana
AU  - Maksimović, Vuk
AU  - Cosic, Tatjana
AU  - Devrnja, Nina
AU  - Tubic, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1406
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars' metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
IS  - 1
VL  - 10
DO  - 10.3390/plants10010172
ER  - 

@article{
author = "Stupar, Sofija and Dragicević, Milan and Tešević, Vele and Stankovic-Jeremic, Jovana and Maksimović, Vuk and Cosic, Tatjana and Devrnja, Nina and Tubic, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars' metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle",
number = "1",
volume = "10",
doi = "10.3390/plants10010172"
}

Stupar, S., Dragicević, M., Tešević, V., Stankovic-Jeremic, J., Maksimović, V., Cosic, T., Devrnja, N., Tubic, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J.. (2021). Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants-Basel
MDPI, Basel., 10(1).
https://doi.org/10.3390/plants10010172

Stupar S, Dragicević M, Tešević V, Stankovic-Jeremic J, Maksimović V, Cosic T, Devrnja N, Tubic L, Cingel A, Vinterhalter B, Ninković S, Savić J. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants-Basel. 2021;10(1).
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragicević, Milan, Tešević, Vele, Stankovic-Jeremic, Jovana, Maksimović, Vuk, Cosic, Tatjana, Devrnja, Nina, Tubic, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena, "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle" in Plants-Basel, 10, no. 1 (2021),
https://doi.org/10.3390/plants10010172 . .

TY  - CONF
AU  - Trailović, Maja
AU  - Dragićević, Ivana
AU  - Raspor, Martin
AU  - Motyka, Vaclav
AU  - Ninković, Slavica
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2436
AB  - Salicilna kiselina (SA) je biljni regulator rastenja čije su glavne fiziološke funkcije odbrana od biotičkog stresa i termogeneza tokom cvetanja kod određenih biljnih vrsta. U novije vreme sve je više podataka o ulozi SA u odbrani biljaka od abiotičkog stresa. SA se često označava kao biljni hormon, mada su podaci o njenim specifičnim efektima na rastenje i razviće kod biljaka sporadični i ograničeni na mali broj vrsta. U literaturi nema podataka o efektima SA na rastenje i razviće krompira, kao ni o interakciji SA sa biljnim hormonima koji regulišu ekonomski značajan proces tuberizacije kod ove vrste. Cilj sprovedenog istraživanja je bio da se utvrdi da li egzogeno primenjena SA ima efekte na morfološke osobine i tuberizaciju kod krompira gajenog in vitro. Ispitivan je efekat SA kod netransformisanog krompira (K) i jedne linije AtCKX2-transformisanog krompira (AtCKX2-51), koju karakteriše veoma nizak sadržaj bioaktivnih citokinina (CK).1 SA je primenjivana u niskim koncentracijama (0,01–10 μM), dodavanjem u podloge za gajenje izdanaka krompira in vitro, a morfološki parametri, uključujući i parametre tuberizacije, određivani su u kulturama starim 30 dana. Rezultati su pokazali da SA (u najvećoj primenjenoj koncentraciji) ima efekat samo na pojedine procese rastenja i razvića, a da ne remeti tuberizaciju. Uočeni efekti se razlikuju kod K i AtCKX2-51 krompira. Prikazani rezultati sprovedenog istraživanja ukazuju na moguću interakciju između SA i CK u regulaciji rastenja i razvića krompira in vitro.
PB  - Srpsko biološko društvo
C3  - DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018.
T1  - Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv Désirée) gajenog in vitro
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2436
ER  - 

@conference{
author = "Trailović, Maja and Dragićević, Ivana and Raspor, Martin and Motyka, Vaclav and Ninković, Slavica",
year = "2018",
abstract = "Salicilna kiselina (SA) je biljni regulator rastenja čije su glavne fiziološke funkcije odbrana od biotičkog stresa i termogeneza tokom cvetanja kod određenih biljnih vrsta. U novije vreme sve je više podataka o ulozi SA u odbrani biljaka od abiotičkog stresa. SA se često označava kao biljni hormon, mada su podaci o njenim specifičnim efektima na rastenje i razviće kod biljaka sporadični i ograničeni na mali broj vrsta. U literaturi nema podataka o efektima SA na rastenje i razviće krompira, kao ni o interakciji SA sa biljnim hormonima koji regulišu ekonomski značajan proces tuberizacije kod ove vrste. Cilj sprovedenog istraživanja je bio da se utvrdi da li egzogeno primenjena SA ima efekte na morfološke osobine i tuberizaciju kod krompira gajenog in vitro. Ispitivan je efekat SA kod netransformisanog krompira (K) i jedne linije AtCKX2-transformisanog krompira (AtCKX2-51), koju karakteriše veoma nizak sadržaj bioaktivnih citokinina (CK).1 SA je primenjivana u niskim koncentracijama (0,01–10 μM), dodavanjem u podloge za gajenje izdanaka krompira in vitro, a morfološki parametri, uključujući i parametre tuberizacije, određivani su u kulturama starim 30 dana. Rezultati su pokazali da SA (u najvećoj primenjenoj koncentraciji) ima efekat samo na pojedine procese rastenja i razvića, a da ne remeti tuberizaciju. Uočeni efekti se razlikuju kod K i AtCKX2-51 krompira. Prikazani rezultati sprovedenog istraživanja ukazuju na moguću interakciju između SA i CK u regulaciji rastenja i razvića krompira in vitro.",
publisher = "Srpsko biološko društvo",
journal = "DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018.",
title = "Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv Désirée) gajenog in vitro",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2436"
}

Trailović, M., Dragićević, I., Raspor, M., Motyka, V.,& Ninković, S.. (2018). Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv Désirée) gajenog in vitro. in DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018.
Srpsko biološko društvo..
https://hdl.handle.net/21.15107/rcub_rimsi_2436

Trailović M, Dragićević I, Raspor M, Motyka V, Ninković S. Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv Désirée) gajenog in vitro. in DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018.. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2436 .

Trailović, Maja, Dragićević, Ivana, Raspor, Martin, Motyka, Vaclav, Ninković, Slavica, "Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv Désirée) gajenog in vitro" in DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018. (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2436 .

TY  - JOUR
AU  - Mitic, Nevena V
AU  - Dmitrović, Slavica
AU  - Đorđević, Mirka
AU  - Zdravkovic-Korac, Snežana R
AU  - Nikolic, Radomirka R
AU  - Raspor, Martin
AU  - Đorđević, Tatjana
AU  - Maksimović, Vuk
AU  - Živković, Suzana
AU  - Krstić-Milošević, Dijana B.
AU  - Stanisic, Mariana
AU  - Ninković, Slavica
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/599
AB  - We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale.
PB  - Elsevier Gmbh, Munich
T2  - Journal of Plant Physiology
T1  - Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays
EP  - 1211
IS  - 12
SP  - 1203
VL  - 169
DO  - 10.1016/j.jplph.2012.04.009
ER  - 

@article{
author = "Mitic, Nevena V and Dmitrović, Slavica and Đorđević, Mirka and Zdravkovic-Korac, Snežana R and Nikolic, Radomirka R and Raspor, Martin and Đorđević, Tatjana and Maksimović, Vuk and Živković, Suzana and Krstić-Milošević, Dijana B. and Stanisic, Mariana and Ninković, Slavica",
year = "2012",
abstract = "We investigated Chenopodium murale transgenic hairy root in vitro culture system as a new tool for allelopathic assays. Transgenic hairy roots were induced by Agrobacterium rhizogenes A4M70GUS from roots, cotyledons, leaves, and internodes of C. murale seedlings. Roots were found to be the best target explants, providing transformation efficiency of up to 11.1%. Established hairy root clones differed in their morphology and growth potential. Molecular characterization of these clones was carried out by PCR, RT-PCR and histochemical GUS analyses. No differences in rol gene expression were observed. Liquid culture system of characterized hairy root clones was maintained for over 2 years. Six hairy root clones were selected for assaying the allelopathic effect of their growth medium against germination and seedling elongation of wheat and lettuce test plants. The inhibitory potential varied depending on the hairy root clone. Some transgenic clones showed significantly higher inhibition compared to wild-type roots. These results revealed that hairy roots as an independent system synthesize some bioactive substances with allelopathic activity and exude them into the growth medium. Concentrations of caffeic, ferulic and p-coumaric acids (0.07-2.85 mu mol/L) identified by HPLC analysis in the growth media were at least 1000 times lower than the inhibitory active concentration (5 mmol/L) of pure grade phenolic acids, suggesting that they have a limited role in the allelopathic phenomena of C murale. The presented hairy root system appears to be a suitable tool for further investigation of the potential and nature of root-mediated allelopathic interference of C. murale.",
publisher = "Elsevier Gmbh, Munich",
journal = "Journal of Plant Physiology",
title = "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays",
pages = "1211-1203",
number = "12",
volume = "169",
doi = "10.1016/j.jplph.2012.04.009"
}

Mitic, N. V., Dmitrović, S., Đorđević, M., Zdravkovic-Korac, S. R., Nikolic, R. R., Raspor, M., Đorđević, T., Maksimović, V., Živković, S., Krstić-Milošević, D. B., Stanisic, M.,& Ninković, S.. (2012). Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology
Elsevier Gmbh, Munich., 169(12), 1203-1211.
https://doi.org/10.1016/j.jplph.2012.04.009

Mitic NV, Dmitrović S, Đorđević M, Zdravkovic-Korac SR, Nikolic RR, Raspor M, Đorđević T, Maksimović V, Živković S, Krstić-Milošević DB, Stanisic M, Ninković S. Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays. in Journal of Plant Physiology. 2012;169(12):1203-1211.
doi:10.1016/j.jplph.2012.04.009 .

Mitic, Nevena V, Dmitrović, Slavica, Đorđević, Mirka, Zdravkovic-Korac, Snežana R, Nikolic, Radomirka R, Raspor, Martin, Đorđević, Tatjana, Maksimović, Vuk, Živković, Suzana, Krstić-Milošević, Dijana B., Stanisic, Mariana, Ninković, Slavica, "Use of Chenopodium murale L. transgenic hairy root in vitro culture system as a new tool for allelopathic assays" in Journal of Plant Physiology, 169, no. 12 (2012):1203-1211,
https://doi.org/10.1016/j.jplph.2012.04.009 . .

TY  - JOUR
AU  - Platisa, Jelena
AU  - Veljović-Jovanović, Sonja
AU  - Kukavica, Biljana
AU  - Vinterhalter, Branka
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/259
AB  - Peroxidase (POD) and superoxide dismutase (SOD) enzyme activities were analyzed in non-regenerative transformed embryogenic lines of alfalfa (Medicago sativa L.) carrying wound-inducible oryzacystatin I (OC-I), wound-inducible oryzacystatin I antisense (OC-las), or hygromycin phosphotransferase (hpt) genes. All of the transformed lines analyzed had elevated levels of all POD isoforms. Three POD isoforms with p/ values of approximately 4.5, 4.8, and 8.4, and one additional pair of isoforms with a p/ value of approximately 8.8 were separated from tissue extracts of all transgenic lines. Isoelectrofocusing, patterns revealed the induction of one isoform of SOD with a p/ of about 5.6 in all transgenic lines compared with non-transformed embryogenic tissue. These results indicate that the process of transformation may disrupt redox homeostasis in alflalfa tissues.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.)
EP  - 900
IS  - 8
SP  - 895
VL  - 165
DO  - 10.1016/j.jplph.2007.07.021
ER  - 

@article{
author = "Platisa, Jelena and Veljović-Jovanović, Sonja and Kukavica, Biljana and Vinterhalter, Branka and Smigocki, Ann and Ninković, Slavica",
year = "2008",
abstract = "Peroxidase (POD) and superoxide dismutase (SOD) enzyme activities were analyzed in non-regenerative transformed embryogenic lines of alfalfa (Medicago sativa L.) carrying wound-inducible oryzacystatin I (OC-I), wound-inducible oryzacystatin I antisense (OC-las), or hygromycin phosphotransferase (hpt) genes. All of the transformed lines analyzed had elevated levels of all POD isoforms. Three POD isoforms with p/ values of approximately 4.5, 4.8, and 8.4, and one additional pair of isoforms with a p/ value of approximately 8.8 were separated from tissue extracts of all transgenic lines. Isoelectrofocusing, patterns revealed the induction of one isoform of SOD with a p/ of about 5.6 in all transgenic lines compared with non-transformed embryogenic tissue. These results indicate that the process of transformation may disrupt redox homeostasis in alflalfa tissues.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.)",
pages = "900-895",
number = "8",
volume = "165",
doi = "10.1016/j.jplph.2007.07.021"
}

Platisa, J., Veljović-Jovanović, S., Kukavica, B., Vinterhalter, B., Smigocki, A.,& Ninković, S.. (2008). Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.). in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 165(8), 895-900.
https://doi.org/10.1016/j.jplph.2007.07.021

Platisa J, Veljović-Jovanović S, Kukavica B, Vinterhalter B, Smigocki A, Ninković S. Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.). in Journal of Plant Physiology. 2008;165(8):895-900.
doi:10.1016/j.jplph.2007.07.021 .

Platisa, Jelena, Veljović-Jovanović, Sonja, Kukavica, Biljana, Vinterhalter, Branka, Smigocki, Ann, Ninković, Slavica, "Induction of peroxidases and superoxide dismutases in transformed embryogenic calli of alfalfa (Medicago sativa L.)" in Journal of Plant Physiology, 165, no. 8 (2008):895-900,
https://doi.org/10.1016/j.jplph.2007.07.021 . .