TY  - JOUR
AU  - Stupar, Sofija
AU  - Dragicević, Milan
AU  - Tešević, Vele
AU  - Stankovic-Jeremic, Jovana
AU  - Maksimović, Vuk
AU  - Cosic, Tatjana
AU  - Devrnja, Nina
AU  - Tubic, Ljiljana
AU  - Cingel, Aleksandar
AU  - Vinterhalter, Branka
AU  - Ninković, Slavica
AU  - Savić, Jelena
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1406
AB  - Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars' metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.
PB  - MDPI, Basel
T2  - Plants-Basel
T1  - Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle
IS  - 1
VL  - 10
DO  - 10.3390/plants10010172
ER  - 

@article{
author = "Stupar, Sofija and Dragicević, Milan and Tešević, Vele and Stankovic-Jeremic, Jovana and Maksimović, Vuk and Cosic, Tatjana and Devrnja, Nina and Tubic, Ljiljana and Cingel, Aleksandar and Vinterhalter, Branka and Ninković, Slavica and Savić, Jelena",
year = "2021",
abstract = "Flower strips of French Marigold are commonly used pest repellents in potato fields. However, the effect of French Marigold volatiles on potato metabolism, physiology and induced defense is unknown. Thus, a microarray transcriptome analysis was performed to study the effects of French Marigold essential oil (EO) on laboratory-grown potato. After 8 h of exposure to EO, with gas chromatography/mass spectrometry (GC/MS)-detected terpinolene and limonene as dominant compounds, 2796 transcripts were differentially expressed with fold change >2 compared to expression in controls. A slightly higher number of transcripts had suppressed expression (1493 down- vs. 1303 up-regulated). Since transcripts, annotated to different photosynthesis-related processes, were mostly down-regulated, we selected a set of 10 genes involved in the leaf starch metabolism pathway, and validated microarray patterns using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Except for decreased synthesis and induced decomposition of starch granule in leaves, 8 h long EO exposure slightly elevated the accumulation of sucrose compared to glucose and fructose in subjected potato plants. An in vitro feeding bioassay with Colorado potato beetle showed that EO-induced alternations on transcriptional level and in the sugars' metabolism caused the enhancement of feeding behavior and overall development of the tested larvae. Results of comprehensive analysis of transcriptional responses in potato exposed to French Marigold EO provide a basis for further elucidation of molecular mechanisms underlying eco-physiological interactions in companion planting cropping systems.",
publisher = "MDPI, Basel",
journal = "Plants-Basel",
title = "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle",
number = "1",
volume = "10",
doi = "10.3390/plants10010172"
}

Stupar, S., Dragicević, M., Tešević, V., Stankovic-Jeremic, J., Maksimović, V., Cosic, T., Devrnja, N., Tubic, L., Cingel, A., Vinterhalter, B., Ninković, S.,& Savić, J.. (2021). Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants-Basel
MDPI, Basel., 10(1).
https://doi.org/10.3390/plants10010172

Stupar S, Dragicević M, Tešević V, Stankovic-Jeremic J, Maksimović V, Cosic T, Devrnja N, Tubic L, Cingel A, Vinterhalter B, Ninković S, Savić J. Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle. in Plants-Basel. 2021;10(1).
doi:10.3390/plants10010172 .

Stupar, Sofija, Dragicević, Milan, Tešević, Vele, Stankovic-Jeremic, Jovana, Maksimović, Vuk, Cosic, Tatjana, Devrnja, Nina, Tubic, Ljiljana, Cingel, Aleksandar, Vinterhalter, Branka, Ninković, Slavica, Savić, Jelena, "Transcriptome Profiling of the Potato Exposed to French Marigold Essential Oil with a Special Emphasis on Leaf Starch Metabolism and Defense against Colorado Potato Beetle" in Plants-Basel, 10, no. 1 (2021),
https://doi.org/10.3390/plants10010172 . .

TY  - JOUR
AU  - Calic, Dusica D
AU  - Bohanec, Borut
AU  - Devrnja, Nina M
AU  - Milojević, Jelena D
AU  - Tubic, Ljiljana
AU  - Kostić, Igor
AU  - Zdravkovic-Korac, Snežana R
PY  - 2013
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/701
AB  - Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.
PB  - Springer Heidelberg, Heidelberg
T2  - Trees-Structure and Function
T1  - Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos
EP  - 762
IS  - 3
SP  - 755
VL  - 27
DO  - 10.1007/s00468-012-0830-4
ER  - 

@article{
author = "Calic, Dusica D and Bohanec, Borut and Devrnja, Nina M and Milojević, Jelena D and Tubic, Ljiljana and Kostić, Igor and Zdravkovic-Korac, Snežana R",
year = "2013",
abstract = "Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Trees-Structure and Function",
title = "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos",
pages = "762-755",
number = "3",
volume = "27",
doi = "10.1007/s00468-012-0830-4"
}

Calic, D. D., Bohanec, B., Devrnja, N. M., Milojević, J. D., Tubic, L., Kostić, I.,& Zdravkovic-Korac, S. R.. (2013). Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function
Springer Heidelberg, Heidelberg., 27(3), 755-762.
https://doi.org/10.1007/s00468-012-0830-4

Calic DD, Bohanec B, Devrnja NM, Milojević JD, Tubic L, Kostić I, Zdravkovic-Korac SR. Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function. 2013;27(3):755-762.
doi:10.1007/s00468-012-0830-4 .

Calic, Dusica D, Bohanec, Borut, Devrnja, Nina M, Milojević, Jelena D, Tubic, Ljiljana, Kostić, Igor, Zdravkovic-Korac, Snežana R, "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos" in Trees-Structure and Function, 27, no. 3 (2013):755-762,
https://doi.org/10.1007/s00468-012-0830-4 . .