TY  - JOUR
AU  - Mattoo, Autar
AU  - Cavigelli, Michel
AU  - Mišić, Danijela
AU  - Gašić, Uroš
AU  - Maksimović, Vuk
AU  - Kramer, Matthew
AU  - Kaur, Bhavneet
AU  - Matekalo, Dragana
AU  - Nestorović Živković, Jasmina
AU  - Roberts, Daniel
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2198
AB  - Maize is important to global food security, being one of the predominant cereals in human and domesticated livestock diets worldwide. Due to the increasing human population, it will be important to not only design cropping systems to increase maize yield and sustainability but also to improve the nutritional quality of maize edible tissues. To determine cropping system impacts on maize grain nutritional content, we sampled grain from conventional and organic maize varieties grown for three growing seasons using five cropping systems. We analyzed the grain using metabolic fingerprinting of methanol extracts with ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS), adopting both non-targeted and targeted approaches. The cropping systems are part of a long-term study at the Beltsville Agricultural Research Center in Beltsville, Maryland, and were a three-year conventional no-till rotation (NT), a three-year conventional chisel-till rotation (CT), a two-year organic rotation (Org2), a three-year organic rotation (Org3), and a six-year organic rotation (Org6). Each cropping system had been in place for at least 10 years, allowing specific cropping-system-induced alterations of soil edaphic and microbial properties. Non-targeted metabolic fingerprinting detected a total of 90 compounds, the majority of which were phenolics. Metabolic profiling was further targeted toward 15 phenolics, 1 phytohormone, 7 carbohydrates and 7 organic acids, which were quantified in the maize grain originating from the five cropping systems. Statistical analysis of this subset of quantitative data determined that cropping system can significantly influence levels of certain maize grain metabolites. However, natural impacts (growing year) were substantially greater than cropping system impacts, likely masking or over-riding some cropping system impacts. Additionally, maize cultivar genetics had greater impact than cropping system on the maize grain metabolome and was the greatest “managed” impact on the metabolite profiles. Results indicate that until natural environmental impacts on maize grain metabolite levels are understood and managed, the best approach to reliably increase maize grain nutritional quality is through development of maize cultivars with enhanced nutritional content that are robust to natural environmental influence.
PB  - Frontiers Media SA
T2  - Frontiers in Sustainable Food Systems
T1  - Maize metabolomics in relation to cropping system and growing year
EP  - 13
SP  - 1
VL  - 7
DO  - 10.3389/fsufs.2023.1130089
ER  - 

@article{
author = "Mattoo, Autar and Cavigelli, Michel and Mišić, Danijela and Gašić, Uroš and Maksimović, Vuk and Kramer, Matthew and Kaur, Bhavneet and Matekalo, Dragana and Nestorović Živković, Jasmina and Roberts, Daniel",
year = "2023",
abstract = "Maize is important to global food security, being one of the predominant cereals in human and domesticated livestock diets worldwide. Due to the increasing human population, it will be important to not only design cropping systems to increase maize yield and sustainability but also to improve the nutritional quality of maize edible tissues. To determine cropping system impacts on maize grain nutritional content, we sampled grain from conventional and organic maize varieties grown for three growing seasons using five cropping systems. We analyzed the grain using metabolic fingerprinting of methanol extracts with ultra-high performance liquid chromatography (UHPLC) coupled with mass spectrometry (MS), adopting both non-targeted and targeted approaches. The cropping systems are part of a long-term study at the Beltsville Agricultural Research Center in Beltsville, Maryland, and were a three-year conventional no-till rotation (NT), a three-year conventional chisel-till rotation (CT), a two-year organic rotation (Org2), a three-year organic rotation (Org3), and a six-year organic rotation (Org6). Each cropping system had been in place for at least 10 years, allowing specific cropping-system-induced alterations of soil edaphic and microbial properties. Non-targeted metabolic fingerprinting detected a total of 90 compounds, the majority of which were phenolics. Metabolic profiling was further targeted toward 15 phenolics, 1 phytohormone, 7 carbohydrates and 7 organic acids, which were quantified in the maize grain originating from the five cropping systems. Statistical analysis of this subset of quantitative data determined that cropping system can significantly influence levels of certain maize grain metabolites. However, natural impacts (growing year) were substantially greater than cropping system impacts, likely masking or over-riding some cropping system impacts. Additionally, maize cultivar genetics had greater impact than cropping system on the maize grain metabolome and was the greatest “managed” impact on the metabolite profiles. Results indicate that until natural environmental impacts on maize grain metabolite levels are understood and managed, the best approach to reliably increase maize grain nutritional quality is through development of maize cultivars with enhanced nutritional content that are robust to natural environmental influence.",
publisher = "Frontiers Media SA",
journal = "Frontiers in Sustainable Food Systems",
title = "Maize metabolomics in relation to cropping system and growing year",
pages = "13-1",
volume = "7",
doi = "10.3389/fsufs.2023.1130089"
}

Mattoo, A., Cavigelli, M., Mišić, D., Gašić, U., Maksimović, V., Kramer, M., Kaur, B., Matekalo, D., Nestorović Živković, J.,& Roberts, D.. (2023). Maize metabolomics in relation to cropping system and growing year. in Frontiers in Sustainable Food Systems
Frontiers Media SA., 7, 1-13.
https://doi.org/10.3389/fsufs.2023.1130089

Mattoo A, Cavigelli M, Mišić D, Gašić U, Maksimović V, Kramer M, Kaur B, Matekalo D, Nestorović Živković J, Roberts D. Maize metabolomics in relation to cropping system and growing year. in Frontiers in Sustainable Food Systems. 2023;7:1-13.
doi:10.3389/fsufs.2023.1130089 .

Mattoo, Autar, Cavigelli, Michel, Mišić, Danijela, Gašić, Uroš, Maksimović, Vuk, Kramer, Matthew, Kaur, Bhavneet, Matekalo, Dragana, Nestorović Živković, Jasmina, Roberts, Daniel, "Maize metabolomics in relation to cropping system and growing year" in Frontiers in Sustainable Food Systems, 7 (2023):1-13,
https://doi.org/10.3389/fsufs.2023.1130089 . .

TY  - JOUR
AU  - Anicic, Neda
AU  - Gasic, Uros
AU  - Lu, Feng
AU  - Ciric, Ana
AU  - Ivanov, Marija
AU  - Jevtic, Bojan
AU  - Dimitrijević, Milena
AU  - Anđelković, Boban
AU  - Skoric, Marijana
AU  - Nestorović-Živković, Jasmina M
AU  - Mao, Yingle
AU  - Liu, Jia
AU  - Tang, Chunping
AU  - Soković, Marina
AU  - Ye, Yang
AU  - Misic, Danijela
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1485
AB  - Two Balkan Peninsula endemics, Nepeta rtanjensis and N. argolica subsp. argolica, both characterized by specialized metabolite profiles predominated by iridoids and phenolics, are differentiated according to the stereochemistry of major iridoid aglycone nepetalactone (NL). For the first time, the present study provides a comparative analysis of antimicrobial and immunomodulating activities of the two Nepeta species and their major iridoids isolated from natural sources-cis,trans-NL, trans,cis-NL, and 1,5,9-epideoxyloganic acid (1,5,9-eDLA), as well as of phenolic acid rosmarinic acid (RA). Methanol extracts and pure iridoids displayed excellent antimicrobial activity against eight strains of bacteria and seven strains of fungi. They were especially potent against food-borne pathogens such as L. monocytogenes, E. coli, S. aureus, Penicillium sp., and Aspergillus sp. Targeted iridoids were efficient agents in preventing biofilm formation of resistant P. aeruginosa strain, and they displayed additive antimicrobial interaction. Iridoids are, to a great extent, responsible for the prominent antimicrobial activities of the two Nepeta species, although are probably minor contributors to the moderate immunomodulatory effects. The analyzed iridoids and RA, individually or in mixtures, have the potential to be used in the pharmaceutical industry as potent antimicrobials, and in the food industry to increase the shelf life and safety of food products.
PB  - MDPI, Basel
T2  - Pharmaceuticals
T1  - Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources
IS  - 5
VL  - 14
DO  - 10.3390/ph14050414
ER  - 

@article{
author = "Anicic, Neda and Gasic, Uros and Lu, Feng and Ciric, Ana and Ivanov, Marija and Jevtic, Bojan and Dimitrijević, Milena and Anđelković, Boban and Skoric, Marijana and Nestorović-Živković, Jasmina M and Mao, Yingle and Liu, Jia and Tang, Chunping and Soković, Marina and Ye, Yang and Misic, Danijela",
year = "2021",
abstract = "Two Balkan Peninsula endemics, Nepeta rtanjensis and N. argolica subsp. argolica, both characterized by specialized metabolite profiles predominated by iridoids and phenolics, are differentiated according to the stereochemistry of major iridoid aglycone nepetalactone (NL). For the first time, the present study provides a comparative analysis of antimicrobial and immunomodulating activities of the two Nepeta species and their major iridoids isolated from natural sources-cis,trans-NL, trans,cis-NL, and 1,5,9-epideoxyloganic acid (1,5,9-eDLA), as well as of phenolic acid rosmarinic acid (RA). Methanol extracts and pure iridoids displayed excellent antimicrobial activity against eight strains of bacteria and seven strains of fungi. They were especially potent against food-borne pathogens such as L. monocytogenes, E. coli, S. aureus, Penicillium sp., and Aspergillus sp. Targeted iridoids were efficient agents in preventing biofilm formation of resistant P. aeruginosa strain, and they displayed additive antimicrobial interaction. Iridoids are, to a great extent, responsible for the prominent antimicrobial activities of the two Nepeta species, although are probably minor contributors to the moderate immunomodulatory effects. The analyzed iridoids and RA, individually or in mixtures, have the potential to be used in the pharmaceutical industry as potent antimicrobials, and in the food industry to increase the shelf life and safety of food products.",
publisher = "MDPI, Basel",
journal = "Pharmaceuticals",
title = "Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources",
number = "5",
volume = "14",
doi = "10.3390/ph14050414"
}

Anicic, N., Gasic, U., Lu, F., Ciric, A., Ivanov, M., Jevtic, B., Dimitrijević, M., Anđelković, B., Skoric, M., Nestorović-Živković, J. M., Mao, Y., Liu, J., Tang, C., Soković, M., Ye, Y.,& Misic, D.. (2021). Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources. in Pharmaceuticals
MDPI, Basel., 14(5).
https://doi.org/10.3390/ph14050414

Anicic N, Gasic U, Lu F, Ciric A, Ivanov M, Jevtic B, Dimitrijević M, Anđelković B, Skoric M, Nestorović-Živković JM, Mao Y, Liu J, Tang C, Soković M, Ye Y, Misic D. Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources. in Pharmaceuticals. 2021;14(5).
doi:10.3390/ph14050414 .

Anicic, Neda, Gasic, Uros, Lu, Feng, Ciric, Ana, Ivanov, Marija, Jevtic, Bojan, Dimitrijević, Milena, Anđelković, Boban, Skoric, Marijana, Nestorović-Živković, Jasmina M, Mao, Yingle, Liu, Jia, Tang, Chunping, Soković, Marina, Ye, Yang, Misic, Danijela, "Antimicrobial and Immunomodulating Activities of Two Endemic Nepeta Species and Their Major Iridoids Isolated from Natural Sources" in Pharmaceuticals, 14, no. 5 (2021),
https://doi.org/10.3390/ph14050414 . .

TY  - JOUR
AU  - Misic, Danijela
AU  - Siler, Branislav
AU  - Nestorović-Živković, Jasmina M
AU  - Simonović, Ana D
AU  - Maksimović, Vuk
AU  - Budimir, Snežana M
AU  - Janosević, Dusica A
AU  - Djuricković, Milutin S
AU  - Nikolic, Miroslav
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/506
AB  - The effect of reduced availability of sugars on growth and essential metabolic processes in roots, resulting from decreased photosynthesis under salinity, was excluded by establishing a non-photosynthetic model-system in this study: root cultures of Centaurium maritimum (L.) Fritch and Centaurium spicatum (L.) Fritch. The contribution of inorganic cations and organic compounds (e. g. carbohydrates and amino acids) to the osmotic adjustment (OA) in roots during short-term exposure to various salt concentrations (0, 50, 100 or 200 mM NaCl) was emphasized. Observed morphological and histological changes in roots were species specific, and were dependent on salinity level. Although C. spicatum appears to be more tolerant to salt stress, both species employed similar strategies in response to elevated salinity to different extents, and displayed effective OA mechanisms. Under low and moderate salinity, inorganic cations were the major contributors to OA in roots of both species, followed by soluble sugars, while the relative contribution of proline (Pro) and free amino acids was insignificant. Osmotic adjustment under severe stress appears to be mediated by increased accumulation of organic compounds. The analysis of the intraspecies variability in salt response of C. spicatum and C. maritimum roots enabled the identification of some organic compounds which could be used as potential biochemical markers in screening for salt tolerance, including Pro in C. spicatum, and trehalose and polyols in C. maritimum.
PB  - Springer, Dordrecht
T2  - Plant Cell Tissue and Organ Culture
T1  - Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress
EP  - 400
IS  - 3
SP  - 389
VL  - 108
DO  - 10.1007/s11240-011-0050-4
ER  - 

@article{
author = "Misic, Danijela and Siler, Branislav and Nestorović-Živković, Jasmina M and Simonović, Ana D and Maksimović, Vuk and Budimir, Snežana M and Janosević, Dusica A and Djuricković, Milutin S and Nikolic, Miroslav",
year = "2012",
abstract = "The effect of reduced availability of sugars on growth and essential metabolic processes in roots, resulting from decreased photosynthesis under salinity, was excluded by establishing a non-photosynthetic model-system in this study: root cultures of Centaurium maritimum (L.) Fritch and Centaurium spicatum (L.) Fritch. The contribution of inorganic cations and organic compounds (e. g. carbohydrates and amino acids) to the osmotic adjustment (OA) in roots during short-term exposure to various salt concentrations (0, 50, 100 or 200 mM NaCl) was emphasized. Observed morphological and histological changes in roots were species specific, and were dependent on salinity level. Although C. spicatum appears to be more tolerant to salt stress, both species employed similar strategies in response to elevated salinity to different extents, and displayed effective OA mechanisms. Under low and moderate salinity, inorganic cations were the major contributors to OA in roots of both species, followed by soluble sugars, while the relative contribution of proline (Pro) and free amino acids was insignificant. Osmotic adjustment under severe stress appears to be mediated by increased accumulation of organic compounds. The analysis of the intraspecies variability in salt response of C. spicatum and C. maritimum roots enabled the identification of some organic compounds which could be used as potential biochemical markers in screening for salt tolerance, including Pro in C. spicatum, and trehalose and polyols in C. maritimum.",
publisher = "Springer, Dordrecht",
journal = "Plant Cell Tissue and Organ Culture",
title = "Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress",
pages = "400-389",
number = "3",
volume = "108",
doi = "10.1007/s11240-011-0050-4"
}

Misic, D., Siler, B., Nestorović-Živković, J. M., Simonović, A. D., Maksimović, V., Budimir, S. M., Janosević, D. A., Djuricković, M. S.,& Nikolic, M.. (2012). Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress. in Plant Cell Tissue and Organ Culture
Springer, Dordrecht., 108(3), 389-400.
https://doi.org/10.1007/s11240-011-0050-4

Misic D, Siler B, Nestorović-Živković JM, Simonović AD, Maksimović V, Budimir SM, Janosević DA, Djuricković MS, Nikolic M. Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress. in Plant Cell Tissue and Organ Culture. 2012;108(3):389-400.
doi:10.1007/s11240-011-0050-4 .

Misic, Danijela, Siler, Branislav, Nestorović-Živković, Jasmina M, Simonović, Ana D, Maksimović, Vuk, Budimir, Snežana M, Janosević, Dusica A, Djuricković, Milutin S, Nikolic, Miroslav, "Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress" in Plant Cell Tissue and Organ Culture, 108, no. 3 (2012):389-400,
https://doi.org/10.1007/s11240-011-0050-4 . .

TY  - JOUR
AU  - Misic, Danijela
AU  - Dragicević, Milan
AU  - Siler, Branislav
AU  - Nestorović-Živković, Jasmina M
AU  - Maksimović, Vuk
AU  - Momcilović, Ivana
AU  - Nikolic, Miroslav
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/542
AB  - A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Plant Physiology
T1  - Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress
EP  - 1289
IS  - 13
SP  - 1281
VL  - 169
DO  - 10.1016/j.jplph.2012.04.018
ER  - 

@article{
author = "Misic, Danijela and Dragicević, Milan and Siler, Branislav and Nestorović-Živković, Jasmina M and Maksimović, Vuk and Momcilović, Ivana and Nikolic, Miroslav",
year = "2012",
abstract = "A heterotrophic model system was established in our studies in order to differentiate the effect of high salt concentrations in external medium on growth and sugar metabolism in roots from the effect of reduced sugar availability resulting from decreased photosynthesis under salinity. Soluble sugar content and the activity of acid invertase in root cultures of salt-tolerant (ST) and salt-sensitive (SS) Schenkia spicata (L.) Mansion genotypes were investigated during exposure to different NaCI concentrations (0-200 mM). Their response to severe salinity was characterized by a metabolic adjustment that led to the accumulation of sucrose (Suc) in root tissues. There was clear evidence that cell wall invertase (CW-Inv) is, the major contributor to the Suc/hexose ratio in roots during exposure to elevated salinity. The results of CW-Inv activity and immunodetection assays in our study suggest that the regulation of CW-Inv expression is most likely achieved in a salt stress dependent manner. Also, NaCI modulated soluble acid invertase (SA-Inv) expression differentially in SS and ST genotypes of S. spicata. Regardless of the salt treatment, genotype, or the amount of enzyme, SA-Inv activity was generally low, indicating regulation at the posttranslational level. The results suggest no direct role of SA-Inv in the regulation of the root tissue carbohydrate pool and therefore in the control of the availability of glucose and fructose for the primary metabolism and/or osmotic adjustment in the present heterotrophic model system, 2012 Elsevier GmbH. All rights reserved.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Plant Physiology",
title = "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress",
pages = "1289-1281",
number = "13",
volume = "169",
doi = "10.1016/j.jplph.2012.04.018"
}

Misic, D., Dragicević, M., Siler, B., Nestorović-Živković, J. M., Maksimović, V., Momcilović, I.,& Nikolic, M.. (2012). Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 169(13), 1281-1289.
https://doi.org/10.1016/j.jplph.2012.04.018

Misic D, Dragicević M, Siler B, Nestorović-Živković JM, Maksimović V, Momcilović I, Nikolic M. Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress. in Journal of Plant Physiology. 2012;169(13):1281-1289.
doi:10.1016/j.jplph.2012.04.018 .

Misic, Danijela, Dragicević, Milan, Siler, Branislav, Nestorović-Živković, Jasmina M, Maksimović, Vuk, Momcilović, Ivana, Nikolic, Miroslav, "Sugars and acid invertase mediate the physiological response of Schenkia spicata root cultures to salt stress" in Journal of Plant Physiology, 169, no. 13 (2012):1281-1289,
https://doi.org/10.1016/j.jplph.2012.04.018 . .