TY  - JOUR
AU  - Tadic, Vojin
AU  - Tadic, Jovan
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Prodanović, Olivera
AU  - Cosic, Tatjana
AU  - Vujcic, Zoran
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1114
AB  - In this study we investigated physiological parameters of stress (enzymatic and non-enzymatic) in lettuce (Lactuca sativa L.) and its hairy roots induced by water solution of phenol. Two varieties of lettuce were examined, Ljubljanska ledenka and Nansen. Plants were grown in water with phenol concentration of 200 mgL(-1), during 10 days. We monitored activity of peroxidases, catalases, polyphenol oxidase and superoxide dismutase, as well as proline and chlorophyll content. We observed a decrease in peroxidases, and increase in activity of catalase, polyphenol oxidase and superoxide dismutase compared to control plants. The concentration of praline was constantly increasing in both lettuce varieties over the course of the experiment. We detected an increase in activity of all monitored enzymes, except polyphenol oxidases, in hairy roots. The hydroponic system provides a useful framework for studying the effect of different harmful substances and its elimination. In such a system, as used in this work for the study of physiological processes in antioxidant protection activated when plant was exposed to phenol, lettuce and its hairy roots can be viewed as tools for water remediation.
PB  - Elsevier, Amsterdam
T2  - Scientia Horticulturae
T1  - Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)- Part 2
EP  - 83
SP  - 71
VL  - 232
DO  - 10.1016/j.scienta.2017.12.024
ER  - 

@article{
author = "Tadic, Vojin and Tadic, Jovan and Milošević, Snežana and Cingel, Aleksandar and Prodanović, Olivera and Cosic, Tatjana and Vujcic, Zoran",
year = "2018",
abstract = "In this study we investigated physiological parameters of stress (enzymatic and non-enzymatic) in lettuce (Lactuca sativa L.) and its hairy roots induced by water solution of phenol. Two varieties of lettuce were examined, Ljubljanska ledenka and Nansen. Plants were grown in water with phenol concentration of 200 mgL(-1), during 10 days. We monitored activity of peroxidases, catalases, polyphenol oxidase and superoxide dismutase, as well as proline and chlorophyll content. We observed a decrease in peroxidases, and increase in activity of catalase, polyphenol oxidase and superoxide dismutase compared to control plants. The concentration of praline was constantly increasing in both lettuce varieties over the course of the experiment. We detected an increase in activity of all monitored enzymes, except polyphenol oxidases, in hairy roots. The hydroponic system provides a useful framework for studying the effect of different harmful substances and its elimination. In such a system, as used in this work for the study of physiological processes in antioxidant protection activated when plant was exposed to phenol, lettuce and its hairy roots can be viewed as tools for water remediation.",
publisher = "Elsevier, Amsterdam",
journal = "Scientia Horticulturae",
title = "Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)- Part 2",
pages = "83-71",
volume = "232",
doi = "10.1016/j.scienta.2017.12.024"
}

Tadic, V., Tadic, J., Milošević, S., Cingel, A., Prodanović, O., Cosic, T.,& Vujcic, Z.. (2018). Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)- Part 2. in Scientia Horticulturae
Elsevier, Amsterdam., 232, 71-83.
https://doi.org/10.1016/j.scienta.2017.12.024

Tadic V, Tadic J, Milošević S, Cingel A, Prodanović O, Cosic T, Vujcic Z. Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)- Part 2. in Scientia Horticulturae. 2018;232:71-83.
doi:10.1016/j.scienta.2017.12.024 .

Tadic, Vojin, Tadic, Jovan, Milošević, Snežana, Cingel, Aleksandar, Prodanović, Olivera, Cosic, Tatjana, Vujcic, Zoran, "Phenol induced physiological stress in hydroponically grown lettuce (Lactuca sativa L.)- Part 2" in Scientia Horticulturae, 232 (2018):71-83,
https://doi.org/10.1016/j.scienta.2017.12.024 . .

TY  - JOUR
AU  - Tadic, Vojin
AU  - Petric, Marija
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Raspor, Martin
AU  - Spasojević, Dragica
AU  - Tadic, Jovan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/800
AB  - In this study we examined the activities of polyphenol oxidase (PPO) and antioxidant enzymes, peroxidase (POX) and catalase (CAT) during lettuce seed germination at different concentrations of phenol. Out of eleven varieties of lettuce, four were chosen according to their germination tolerance to phenol as follows: plants exhibiting high (Ljubljanska ledenka - LJL and Nansen - N) and low toleranace (Little Gem - LG and Majska kraljica - MK). A decrease in germination efficiency after exposure to LD50 of phenol was determined for these four varieties. The effects of phenol treatment on POX, CAT and PPO activities were determined after 4, 5, 6, 7 and 8 days of growth at LD50 concentrations. A trend of increased peroxidase activity was observed in seeds grown on LD50 of phenol compared to control seeds. A significant increase in CAT activity was observed at the beginning of treatment for MK, LG and N in seeds grown on phenol as well as in control seeds. A trend of increased PPO activity was observed in all control seeds. We also investigated the affinity of PPO for two different substrates that were used for the determination of enzyme activity. Our results show that LJL and N are the varieties most tolerant to growth on phenol. Here we report on the activities of their antioxidant enzymes and PPO during seed germination.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce
EP  - 1514
IS  - 4
SP  - 1503
VL  - 66
DO  - 10.2298/ABS1404503T
ER  - 

@article{
author = "Tadic, Vojin and Petric, Marija and Milošević, Snežana and Cingel, Aleksandar and Raspor, Martin and Spasojević, Dragica and Tadic, Jovan",
year = "2014",
abstract = "In this study we examined the activities of polyphenol oxidase (PPO) and antioxidant enzymes, peroxidase (POX) and catalase (CAT) during lettuce seed germination at different concentrations of phenol. Out of eleven varieties of lettuce, four were chosen according to their germination tolerance to phenol as follows: plants exhibiting high (Ljubljanska ledenka - LJL and Nansen - N) and low toleranace (Little Gem - LG and Majska kraljica - MK). A decrease in germination efficiency after exposure to LD50 of phenol was determined for these four varieties. The effects of phenol treatment on POX, CAT and PPO activities were determined after 4, 5, 6, 7 and 8 days of growth at LD50 concentrations. A trend of increased peroxidase activity was observed in seeds grown on LD50 of phenol compared to control seeds. A significant increase in CAT activity was observed at the beginning of treatment for MK, LG and N in seeds grown on phenol as well as in control seeds. A trend of increased PPO activity was observed in all control seeds. We also investigated the affinity of PPO for two different substrates that were used for the determination of enzyme activity. Our results show that LJL and N are the varieties most tolerant to growth on phenol. Here we report on the activities of their antioxidant enzymes and PPO during seed germination.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce",
pages = "1514-1503",
number = "4",
volume = "66",
doi = "10.2298/ABS1404503T"
}

Tadic, V., Petric, M., Milošević, S., Cingel, A., Raspor, M., Spasojević, D.,& Tadic, J.. (2014). Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(4), 1503-1514.
https://doi.org/10.2298/ABS1404503T

Tadic V, Petric M, Milošević S, Cingel A, Raspor M, Spasojević D, Tadic J. Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce. in Archives of Biological Sciences. 2014;66(4):1503-1514.
doi:10.2298/ABS1404503T .

Tadic, Vojin, Petric, Marija, Milošević, Snežana, Cingel, Aleksandar, Raspor, Martin, Spasojević, Dragica, Tadic, Jovan, "Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce" in Archives of Biological Sciences, 66, no. 4 (2014):1503-1514,
https://doi.org/10.2298/ABS1404503T . .