TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Janakiev, Tamara
AU  - Unković, Nikola
AU  - Ljaljević Grbić, Milica
AU  - Degrassi, Giuliano
AU  - Dimkić, Ivica
PY  - 2024
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/3205
AB  - The soybean is recognized worldwide as one of the most important crops due to its protein- and
oil-rich seeds. Several beneficial bacterial strains, including those of the genus Pseudomonas, are
known to increase plant yield and disease tolerance. The aim of this study was to test the potential of 15 strains of Pseudomonas spp. to promote soybean growth under field conditions. Strains were identified based on 16S rRNA and gyrB sequences, and strains belonging to risk group 1 were further analyzed. Selected strains were tested for plant growth-promoting (PGP) properties, biofilm formation and in vitro antimicrobial activity against various fungal pathogens. PGP activity indicators of the selected strains were evaluated on soybean plants grown in fertilizer-free soil and in soil treated with 70% and 100% fertilizers. Three Pseudomonas spp. strains, P. putida VB131A, P. fulva VB134B and P. rhodesiae VB143S, were selected as PGP candidates. All three strains showed the ability to produce IAA and solubilize phosphorus, while only P. rhodesiae VB143S showed strong lipase and protease activity. Only P. fulva VB134B showed the ability to
produce biofilm and hydrogen cyanide. All three strains showed in vitro antifungal activity against
Alternaria infectoria. P. fulva VB134B and P. rhodesiae VB143S showed antifungal activity against
Alternaria alternata and P. putida VB131A and P. rhodesiae VB143S against Epicoccum nigrum. Only P. fulva VB134B showed activity against Monilinia laxa. Statistically significant differences in
plant height were not observed regardless of the strain or soil treatment used. In terms of first
pod height, the P. fulva VB134B treatment was most effective when applied to soybeans grown
in 100% fertilized soil. In contrast, plants treated with P. rhodesiae VB143S and grown in 100% fertilized soil had the highest number of branches and pods per plant.
PB  - Serbian Society for Microbiology
C3  - XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health"
T1  - Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions
EP  - 70
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_3205
ER  - 

@conference{
author = "Jelušić, Aleksandra and Janakiev, Tamara and Unković, Nikola and Ljaljević Grbić, Milica and Degrassi, Giuliano and Dimkić, Ivica",
year = "2024",
abstract = "The soybean is recognized worldwide as one of the most important crops due to its protein- and
oil-rich seeds. Several beneficial bacterial strains, including those of the genus Pseudomonas, are
known to increase plant yield and disease tolerance. The aim of this study was to test the potential of 15 strains of Pseudomonas spp. to promote soybean growth under field conditions. Strains were identified based on 16S rRNA and gyrB sequences, and strains belonging to risk group 1 were further analyzed. Selected strains were tested for plant growth-promoting (PGP) properties, biofilm formation and in vitro antimicrobial activity against various fungal pathogens. PGP activity indicators of the selected strains were evaluated on soybean plants grown in fertilizer-free soil and in soil treated with 70% and 100% fertilizers. Three Pseudomonas spp. strains, P. putida VB131A, P. fulva VB134B and P. rhodesiae VB143S, were selected as PGP candidates. All three strains showed the ability to produce IAA and solubilize phosphorus, while only P. rhodesiae VB143S showed strong lipase and protease activity. Only P. fulva VB134B showed the ability to
produce biofilm and hydrogen cyanide. All three strains showed in vitro antifungal activity against
Alternaria infectoria. P. fulva VB134B and P. rhodesiae VB143S showed antifungal activity against
Alternaria alternata and P. putida VB131A and P. rhodesiae VB143S against Epicoccum nigrum. Only P. fulva VB134B showed activity against Monilinia laxa. Statistically significant differences in
plant height were not observed regardless of the strain or soil treatment used. In terms of first
pod height, the P. fulva VB134B treatment was most effective when applied to soybeans grown
in 100% fertilized soil. In contrast, plants treated with P. rhodesiae VB143S and grown in 100% fertilized soil had the highest number of branches and pods per plant.",
publisher = "Serbian Society for Microbiology",
journal = "XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health"",
title = "Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_3205"
}

Jelušić, A., Janakiev, T., Unković, N., Ljaljević Grbić, M., Degrassi, G.,& Dimkić, I.. (2024). Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions. in XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health"
Serbian Society for Microbiology., 70-70.
https://hdl.handle.net/21.15107/rcub_rimsi_3205

Jelušić A, Janakiev T, Unković N, Ljaljević Grbić M, Degrassi G, Dimkić I. Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions. in XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health". 2024;:70-70.
https://hdl.handle.net/21.15107/rcub_rimsi_3205 .

Jelušić, Aleksandra, Janakiev, Tamara, Unković, Nikola, Ljaljević Grbić, Milica, Degrassi, Giuliano, Dimkić, Ivica, "Assessment of growth-promoting properties of Pseudomonas spp. on soybeans under field conditions" in XIII CONGRESS OF MICROBIOLOGISTS OF SERBIA with international participation, UMS 24 - MIKROMED REGIO 5 "From biotechnology to human and planetary health" (2024):70-70,
https://hdl.handle.net/21.15107/rcub_rimsi_3205 .

TY  - CONF
AU  - Dimkić, Ivica
AU  - Jelušić, Aleksandra
AU  - Hladnik, Matjaž
AU  - Janakiev, Tamara
AU  - Bandelj, Dunja
AU  - Vukićević, Slavoljub
AU  - Fira, Đorđe
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2041
AB  - Maize (Zea mays L.) occupies a significant part of the world cultivated area. Beneficial microorganisms and organic fertilizers are powerful tools for sustainable agricultural management. Applying appropriate formulation either through seed coating or composting material together with seeds during the sowing was aimed to achieve optimal performance on plants and increase the crop yields. Also, a complete analysis of phytobiome including high-throughput amplicon and whole-genome sequencing, and computational biology of the treated and non-treated plants' roots and soil was applied. The statistically significant difference in bacterial communities between phenophases and treatments was much more prevalent in the roots than soil itself. In general, seedling phenophase (II) differed in terms of diversity from bacterial communities of flowering (III) and harvesting phenophases (IV), regardless of treatment, indicating good colonization with the treated substrate for up to one month. In the root samples, the dominance of the genus Pseudomonas was evident, in the initial stages (phase II), while this percentage decreased linearly over time. High relative abundance of the genera Achromobacter, Sphingobacterium, and Stenotrophomonas also characterized II phenophase. Contrary, a relative abundance of the genus Bacillus was characteristic for III phenophase, and especially twice or three times higher in treatments with phytobiotic or manure compared to the negative control. Furthermore, it was observed that with increasing inoculation of Bacillus isolate, the percentage of Pantoea genus decreases drastically in coated seeds. In conclusion, a statistically significant increase in maize yield was obtained in the treatment with phytobiotic with an average value of 650 kg/ha compared to the negative control.
PB  - Istočno Sarajevo : Poljoprivredni fakultet
C3  - XIII International Scientific Agriculture Symposium "AGROSYM 2022", Jahorina, Bosnia and Herzegovina
T1  - Bioinoculants in sustainable agriculture – the influence on maize characteristics and phytobiome
EP  - 348
SP  - 348
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2041
ER  - 

@conference{
author = "Dimkić, Ivica and Jelušić, Aleksandra and Hladnik, Matjaž and Janakiev, Tamara and Bandelj, Dunja and Vukićević, Slavoljub and Fira, Đorđe",
year = "2022",
abstract = "Maize (Zea mays L.) occupies a significant part of the world cultivated area. Beneficial microorganisms and organic fertilizers are powerful tools for sustainable agricultural management. Applying appropriate formulation either through seed coating or composting material together with seeds during the sowing was aimed to achieve optimal performance on plants and increase the crop yields. Also, a complete analysis of phytobiome including high-throughput amplicon and whole-genome sequencing, and computational biology of the treated and non-treated plants' roots and soil was applied. The statistically significant difference in bacterial communities between phenophases and treatments was much more prevalent in the roots than soil itself. In general, seedling phenophase (II) differed in terms of diversity from bacterial communities of flowering (III) and harvesting phenophases (IV), regardless of treatment, indicating good colonization with the treated substrate for up to one month. In the root samples, the dominance of the genus Pseudomonas was evident, in the initial stages (phase II), while this percentage decreased linearly over time. High relative abundance of the genera Achromobacter, Sphingobacterium, and Stenotrophomonas also characterized II phenophase. Contrary, a relative abundance of the genus Bacillus was characteristic for III phenophase, and especially twice or three times higher in treatments with phytobiotic or manure compared to the negative control. Furthermore, it was observed that with increasing inoculation of Bacillus isolate, the percentage of Pantoea genus decreases drastically in coated seeds. In conclusion, a statistically significant increase in maize yield was obtained in the treatment with phytobiotic with an average value of 650 kg/ha compared to the negative control.",
publisher = "Istočno Sarajevo : Poljoprivredni fakultet",
journal = "XIII International Scientific Agriculture Symposium "AGROSYM 2022", Jahorina, Bosnia and Herzegovina",
title = "Bioinoculants in sustainable agriculture – the influence on maize characteristics and phytobiome",
pages = "348-348",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2041"
}

Dimkić, I., Jelušić, A., Hladnik, M., Janakiev, T., Bandelj, D., Vukićević, S.,& Fira, Đ.. (2022). Bioinoculants in sustainable agriculture – the influence on maize characteristics and phytobiome. in XIII International Scientific Agriculture Symposium "AGROSYM 2022", Jahorina, Bosnia and Herzegovina
Istočno Sarajevo : Poljoprivredni fakultet., 348-348.
https://hdl.handle.net/21.15107/rcub_rimsi_2041

Dimkić I, Jelušić A, Hladnik M, Janakiev T, Bandelj D, Vukićević S, Fira Đ. Bioinoculants in sustainable agriculture – the influence on maize characteristics and phytobiome. in XIII International Scientific Agriculture Symposium "AGROSYM 2022", Jahorina, Bosnia and Herzegovina. 2022;:348-348.
https://hdl.handle.net/21.15107/rcub_rimsi_2041 .

Dimkić, Ivica, Jelušić, Aleksandra, Hladnik, Matjaž, Janakiev, Tamara, Bandelj, Dunja, Vukićević, Slavoljub, Fira, Đorđe, "Bioinoculants in sustainable agriculture – the influence on maize characteristics and phytobiome" in XIII International Scientific Agriculture Symposium "AGROSYM 2022", Jahorina, Bosnia and Herzegovina (2022):348-348,
https://hdl.handle.net/21.15107/rcub_rimsi_2041 .