TY  - CONF
AU  - Antonić, Dragana
AU  - Trailović, Maja
AU  - Trajković, Milena
AU  - Cingel, Aleksandar
AU  - Subotić, Angelina
AU  - Jevremović, Slađana
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2380
AB  - Impatiens hawkeri (Balsaminaceae) je ukrasna biljna vrsta poreklom sa Nove Gvineje. Krioprezervacija predstavlja metod za dugotrajno čuvanje biljnog materijala na -196C u tečnom azotu koji se koristi i za eliminaciju patogena kod biljaka.1 Vrste roda Impatiens su izrazito osetljive na tretmane dehidratacije koji prethode zamrzavanju.2 Takođe, koncentracija saharoze u hranljivim podlogama utiče na morfogenetski potencijal tokom gajenja u kulturi in vitro.3 U ovom radu smo ispitivali uticaj prekulture vrhova izdanaka na povišenoj koncentraciji saharoze (6%), kao i vitamina C na preživljavanje i regeneraciju biljaka posle hemijske dehidratacije vrhova izdanaka sa rastvorima za vitrifikaciju (PVS2 i PVS3). Vrhovi izdanaka I. hawkeri su izrazito osetljivi na tretman sa 100% PVS2 rastvorom. Najveći morfogenetski potencijal posle izlaganja PVS2 rastvoru su pokazali vrhovi tretirani sa 50% PVS2 rastvorom kada je dolazilo samo do kalusiranja vrhova izdanaka. Potpuna regeneracija biljaka posle PVS2 tretmana postignuta je uz dodatak vitamina C u hranljivoj podlozi u koncentraciji od 100 mg/l. Najbolji rezultati preživljavanja i regeneracije biljaka posle odmrzavanja postignuti su korišćenjem metode vitrifikacije sa 100% PVS3 rastvorom u trajanju od 45 min. Izdanci gajeni na hranljivoj podlozi sa 6% saharoze su pokazali veće preživljavanje (49,4%) posle izlaganja ultra niskim temperaturama u odnosu na izdanke gajene na podlogama sa 3% saharoze (26,5 %).
PB  - Srpsko biološko društvo
C3  - DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018.
T1  - Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2380
ER  - 

@conference{
author = "Antonić, Dragana and Trailović, Maja and Trajković, Milena and Cingel, Aleksandar and Subotić, Angelina and Jevremović, Slađana",
year = "2018",
abstract = "Impatiens hawkeri (Balsaminaceae) je ukrasna biljna vrsta poreklom sa Nove Gvineje. Krioprezervacija predstavlja metod za dugotrajno čuvanje biljnog materijala na -196C u tečnom azotu koji se koristi i za eliminaciju patogena kod biljaka.1 Vrste roda Impatiens su izrazito osetljive na tretmane dehidratacije koji prethode zamrzavanju.2 Takođe, koncentracija saharoze u hranljivim podlogama utiče na morfogenetski potencijal tokom gajenja u kulturi in vitro.3 U ovom radu smo ispitivali uticaj prekulture vrhova izdanaka na povišenoj koncentraciji saharoze (6%), kao i vitamina C na preživljavanje i regeneraciju biljaka posle hemijske dehidratacije vrhova izdanaka sa rastvorima za vitrifikaciju (PVS2 i PVS3). Vrhovi izdanaka I. hawkeri su izrazito osetljivi na tretman sa 100% PVS2 rastvorom. Najveći morfogenetski potencijal posle izlaganja PVS2 rastvoru su pokazali vrhovi tretirani sa 50% PVS2 rastvorom kada je dolazilo samo do kalusiranja vrhova izdanaka. Potpuna regeneracija biljaka posle PVS2 tretmana postignuta je uz dodatak vitamina C u hranljivoj podlozi u koncentraciji od 100 mg/l. Najbolji rezultati preživljavanja i regeneracije biljaka posle odmrzavanja postignuti su korišćenjem metode vitrifikacije sa 100% PVS3 rastvorom u trajanju od 45 min. Izdanci gajeni na hranljivoj podlozi sa 6% saharoze su pokazali veće preživljavanje (49,4%) posle izlaganja ultra niskim temperaturama u odnosu na izdanke gajene na podlogama sa 3% saharoze (26,5 %).",
publisher = "Srpsko biološko društvo",
journal = "DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018.",
title = "Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2380"
}

Antonić, D., Trailović, M., Trajković, M., Cingel, A., Subotić, A.,& Jevremović, S.. (2018). Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull. in DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018.
Srpsko biološko društvo..
https://hdl.handle.net/21.15107/rcub_rimsi_2380

Antonić D, Trailović M, Trajković M, Cingel A, Subotić A, Jevremović S. Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull. in DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018.. 2018;.
https://hdl.handle.net/21.15107/rcub_rimsi_2380 .

Antonić, Dragana, Trailović, Maja, Trajković, Milena, Cingel, Aleksandar, Subotić, Angelina, Jevremović, Slađana, "Krioprezervacija vrhova izdanaka Impatiens hawkeri W. Bull" in DRUGI KONGRES BIOLOGA SRBIJE osnovna i primenjena istraživanja metodika nastave, Kladovo, Srbija 25–30.09.2018. (2018),
https://hdl.handle.net/21.15107/rcub_rimsi_2380 .

TY  - JOUR
AU  - Tadic, Vojin
AU  - Petric, Marija
AU  - Milošević, Snežana
AU  - Cingel, Aleksandar
AU  - Raspor, Martin
AU  - Spasojević, Dragica
AU  - Tadic, Jovan
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/800
AB  - In this study we examined the activities of polyphenol oxidase (PPO) and antioxidant enzymes, peroxidase (POX) and catalase (CAT) during lettuce seed germination at different concentrations of phenol. Out of eleven varieties of lettuce, four were chosen according to their germination tolerance to phenol as follows: plants exhibiting high (Ljubljanska ledenka - LJL and Nansen - N) and low toleranace (Little Gem - LG and Majska kraljica - MK). A decrease in germination efficiency after exposure to LD50 of phenol was determined for these four varieties. The effects of phenol treatment on POX, CAT and PPO activities were determined after 4, 5, 6, 7 and 8 days of growth at LD50 concentrations. A trend of increased peroxidase activity was observed in seeds grown on LD50 of phenol compared to control seeds. A significant increase in CAT activity was observed at the beginning of treatment for MK, LG and N in seeds grown on phenol as well as in control seeds. A trend of increased PPO activity was observed in all control seeds. We also investigated the affinity of PPO for two different substrates that were used for the determination of enzyme activity. Our results show that LJL and N are the varieties most tolerant to growth on phenol. Here we report on the activities of their antioxidant enzymes and PPO during seed germination.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce
EP  - 1514
IS  - 4
SP  - 1503
VL  - 66
DO  - 10.2298/ABS1404503T
ER  - 

@article{
author = "Tadic, Vojin and Petric, Marija and Milošević, Snežana and Cingel, Aleksandar and Raspor, Martin and Spasojević, Dragica and Tadic, Jovan",
year = "2014",
abstract = "In this study we examined the activities of polyphenol oxidase (PPO) and antioxidant enzymes, peroxidase (POX) and catalase (CAT) during lettuce seed germination at different concentrations of phenol. Out of eleven varieties of lettuce, four were chosen according to their germination tolerance to phenol as follows: plants exhibiting high (Ljubljanska ledenka - LJL and Nansen - N) and low toleranace (Little Gem - LG and Majska kraljica - MK). A decrease in germination efficiency after exposure to LD50 of phenol was determined for these four varieties. The effects of phenol treatment on POX, CAT and PPO activities were determined after 4, 5, 6, 7 and 8 days of growth at LD50 concentrations. A trend of increased peroxidase activity was observed in seeds grown on LD50 of phenol compared to control seeds. A significant increase in CAT activity was observed at the beginning of treatment for MK, LG and N in seeds grown on phenol as well as in control seeds. A trend of increased PPO activity was observed in all control seeds. We also investigated the affinity of PPO for two different substrates that were used for the determination of enzyme activity. Our results show that LJL and N are the varieties most tolerant to growth on phenol. Here we report on the activities of their antioxidant enzymes and PPO during seed germination.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce",
pages = "1514-1503",
number = "4",
volume = "66",
doi = "10.2298/ABS1404503T"
}

Tadic, V., Petric, M., Milošević, S., Cingel, A., Raspor, M., Spasojević, D.,& Tadic, J.. (2014). Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 66(4), 1503-1514.
https://doi.org/10.2298/ABS1404503T

Tadic V, Petric M, Milošević S, Cingel A, Raspor M, Spasojević D, Tadic J. Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce. in Archives of Biological Sciences. 2014;66(4):1503-1514.
doi:10.2298/ABS1404503T .

Tadic, Vojin, Petric, Marija, Milošević, Snežana, Cingel, Aleksandar, Raspor, Martin, Spasojević, Dragica, Tadic, Jovan, "Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce" in Archives of Biological Sciences, 66, no. 4 (2014):1503-1514,
https://doi.org/10.2298/ABS1404503T . .