TY  - JOUR
AU  - Smederevac-Lalić, Marija
AU  - Cvijanović, Gorčin
AU  - Nikolić, Dušan
AU  - Jaćimović, Milica
AU  - Skorić, Stefan
AU  - Višnjić Jeftić, Željka
AU  - Hegediš, Aleksandar
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2931
AB  - Over 2850 km long, the Danube River is certainly the most important river in Europe, flowing through Germany (687 km), Austria (358 km), Slovakia (172 km), Hungary (417 km), Croatia (138 km), Serbia (587 km), Bulgaria (472 km), Romania (1075 km), Moldovia (<1 km) and Ukraine (54 km) in a west-east direction, and representing a “water backbone” for many European countries located in its basin.
PB  - International Society of Limnology
T2  - International Society of Limnology
T1  - The Danube River in Serbia
IS  - 82
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2931
ER  - 

@article{
author = "Smederevac-Lalić, Marija and Cvijanović, Gorčin and Nikolić, Dušan and Jaćimović, Milica and Skorić, Stefan and Višnjić Jeftić, Željka and Hegediš, Aleksandar",
year = "2023",
abstract = "Over 2850 km long, the Danube River is certainly the most important river in Europe, flowing through Germany (687 km), Austria (358 km), Slovakia (172 km), Hungary (417 km), Croatia (138 km), Serbia (587 km), Bulgaria (472 km), Romania (1075 km), Moldovia (<1 km) and Ukraine (54 km) in a west-east direction, and representing a “water backbone” for many European countries located in its basin.",
publisher = "International Society of Limnology",
journal = "International Society of Limnology",
title = "The Danube River in Serbia",
number = "82",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2931"
}

Smederevac-Lalić, M., Cvijanović, G., Nikolić, D., Jaćimović, M., Skorić, S., Višnjić Jeftić, Ž.,& Hegediš, A.. (2023). The Danube River in Serbia. in International Society of Limnology
International Society of Limnology.(82).
https://hdl.handle.net/21.15107/rcub_rimsi_2931

Smederevac-Lalić M, Cvijanović G, Nikolić D, Jaćimović M, Skorić S, Višnjić Jeftić Ž, Hegediš A. The Danube River in Serbia. in International Society of Limnology. 2023;(82).
https://hdl.handle.net/21.15107/rcub_rimsi_2931 .

Smederevac-Lalić, Marija, Cvijanović, Gorčin, Nikolić, Dušan, Jaćimović, Milica, Skorić, Stefan, Višnjić Jeftić, Željka, Hegediš, Aleksandar, "The Danube River in Serbia" in International Society of Limnology, no. 82 (2023),
https://hdl.handle.net/21.15107/rcub_rimsi_2931 .

TY  - JOUR
AU  - Jaćimović, Milica
AU  - Smederevac-Lalić, Marija
AU  - Nikolić, Dušan
AU  - Cvijanović, Gorčin
AU  - Spasić, Slađana
AU  - Višnjić Jeftić, Željka
AU  - Skorić, Stefan
AU  - Krpo-Ćetković, Jasmina
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2427
AB  - 1.	Black bullhead is among the most abundant and successful non-native fish species in European freshwaters. A number of studies have indicated that its physical removal could be a potential solution to minimize possible negative impacts that it may have on native fishes; however, so far there have been no field studies to confirm this.
2.	This study quantified population dynamics of both native and non-native fishes in a small slow-running lowland river within the Ponjavica Nature Park in Serbia before and after the black bullhead removal with fyke-nets, which proved to be an efficient tool for this purpose.
3.	The removal of black bullhead resulted in statistically significant changes in two native species abundance – an increase of rudd (Scardinius erythrophthalmus) (by 5.3 times), and a decline of bleak (Alburnus alburnus) (by 2.3 times).
4.	Significant increase was observed in three non-native fish species: the abundances of topmouth gudgeon (Pseudorasbora parva), pumpkinseed (Lepomis gibbosus), and Prussian carp (Carassius gibelio) increased 25.5, 4.9, and 4.2 times, respectively.
5.	There were also substantial changes in the diversity of the fish assemblage after the black bullhead removal, illustrated by changes in diversity indices: Shannon's index doubled, while the Simpson’s dominance index decreased threefold.
6.	The catch-per-unit-effort and the biomass-per-unit-effort values indicated the dominance of the black bullhead in the fish assemblage. YOY/adult ratio did not vary in relation to abundance, which confirmed that black bullhead might not compensate for fishing mortality with the increased recruitment.
7.	The results suggest that black bullhead plays a significant role in structuring the fish assemblage and that its removal may have both positive and negative effects on species abundance and diversity. These findings can contribute to the conservation of native fish species and improvement of future management programs.
PB  - John  Wiley  &  Sons,  Ltd.
T2  - Aquatic Conservation: Marine and Freshwater Ecosystems
T1  - Changes to fish assemblage following the selective removal of black bullhead (Ameiurus melas)
EP  - 994
IS  - 9
SP  - 981
VL  - 33
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2427
ER  - 

@article{
author = "Jaćimović, Milica and Smederevac-Lalić, Marija and Nikolić, Dušan and Cvijanović, Gorčin and Spasić, Slađana and Višnjić Jeftić, Željka and Skorić, Stefan and Krpo-Ćetković, Jasmina",
year = "2023",
abstract = "1.	Black bullhead is among the most abundant and successful non-native fish species in European freshwaters. A number of studies have indicated that its physical removal could be a potential solution to minimize possible negative impacts that it may have on native fishes; however, so far there have been no field studies to confirm this.
2.	This study quantified population dynamics of both native and non-native fishes in a small slow-running lowland river within the Ponjavica Nature Park in Serbia before and after the black bullhead removal with fyke-nets, which proved to be an efficient tool for this purpose.
3.	The removal of black bullhead resulted in statistically significant changes in two native species abundance – an increase of rudd (Scardinius erythrophthalmus) (by 5.3 times), and a decline of bleak (Alburnus alburnus) (by 2.3 times).
4.	Significant increase was observed in three non-native fish species: the abundances of topmouth gudgeon (Pseudorasbora parva), pumpkinseed (Lepomis gibbosus), and Prussian carp (Carassius gibelio) increased 25.5, 4.9, and 4.2 times, respectively.
5.	There were also substantial changes in the diversity of the fish assemblage after the black bullhead removal, illustrated by changes in diversity indices: Shannon's index doubled, while the Simpson’s dominance index decreased threefold.
6.	The catch-per-unit-effort and the biomass-per-unit-effort values indicated the dominance of the black bullhead in the fish assemblage. YOY/adult ratio did not vary in relation to abundance, which confirmed that black bullhead might not compensate for fishing mortality with the increased recruitment.
7.	The results suggest that black bullhead plays a significant role in structuring the fish assemblage and that its removal may have both positive and negative effects on species abundance and diversity. These findings can contribute to the conservation of native fish species and improvement of future management programs.",
publisher = "John  Wiley  &  Sons,  Ltd.",
journal = "Aquatic Conservation: Marine and Freshwater Ecosystems",
title = "Changes to fish assemblage following the selective removal of black bullhead (Ameiurus melas)",
pages = "994-981",
number = "9",
volume = "33",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2427"
}

Jaćimović, M., Smederevac-Lalić, M., Nikolić, D., Cvijanović, G., Spasić, S., Višnjić Jeftić, Ž., Skorić, S.,& Krpo-Ćetković, J.. (2023). Changes to fish assemblage following the selective removal of black bullhead (Ameiurus melas). in Aquatic Conservation: Marine and Freshwater Ecosystems
John  Wiley  &  Sons,  Ltd.., 33(9), 981-994.
https://hdl.handle.net/21.15107/rcub_rimsi_2427

Jaćimović M, Smederevac-Lalić M, Nikolić D, Cvijanović G, Spasić S, Višnjić Jeftić Ž, Skorić S, Krpo-Ćetković J. Changes to fish assemblage following the selective removal of black bullhead (Ameiurus melas). in Aquatic Conservation: Marine and Freshwater Ecosystems. 2023;33(9):981-994.
https://hdl.handle.net/21.15107/rcub_rimsi_2427 .

Jaćimović, Milica, Smederevac-Lalić, Marija, Nikolić, Dušan, Cvijanović, Gorčin, Spasić, Slađana, Višnjić Jeftić, Željka, Skorić, Stefan, Krpo-Ćetković, Jasmina, "Changes to fish assemblage following the selective removal of black bullhead (Ameiurus melas)" in Aquatic Conservation: Marine and Freshwater Ecosystems, 33, no. 9 (2023):981-994,
https://hdl.handle.net/21.15107/rcub_rimsi_2427 .

TY  - CONF
AU  - Jaćimović, Milica
AU  - Nikolić, Dušan
AU  - Cvijanović, Gorčin
AU  - Višnjić Jeftić, Željka
AU  - Skorić, Stefan
AU  - Smederevac-Lalić, Marija
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2371
AB  - In the sake of better understanding of the mechanisms that drive interactions between
invasive species and their ecosystems, results of selective removal of the black bullhead
(Ameiurus melas) in two different lentic systems were compared. Ponjavica Nature Park has
characteristical watercourse of the plains with very slow flow and the coastal remains of
wetlands. Markovačko reservoir was built for the needs of the irrigation system of the
nearby agricultural land. Both systems suffer dominance of the black bullhead,
characterized as a species of "globally high risk". Combining results of the mass removal,
the effects on the native fish populations have been empirically demonstrated and
quantified. Black bullhead selective removal in Ponjavica lasted from August - October
2018, April - September 2019, with 20,145 black bullhead individuals removed. It showed
decreased abundance of bleak (Alburnus alburnus) 2.3 times, while the abundances of
roach (Rutilus rutilus) and European perch (Perca fluviatilis) remained almost unchanged. A
significant increase in abundance was recorded in rudd (Scardinius erythrophthalmus) (5.3
times). The abundances of topmouth gudgeon (Pseudorasbora parva), pumpkinseed
(Lepomis gibbosus), and Prussian carp (Carassius gibelio) increased 25.5, 4.9, and 4.2 times,
respectively. The mass removal of black bullhead on Markovačko Lake lasted from August -
October 2020, April - September 2021, and 15,921 black bullhead specimens were
removed. When the post removal state from Markovačko lake was determined, two species
appeared in the sample for the first time: European perch and chub (Squalius cephalus). The
abundance of bleak increased 3 times, abundance of Prussian carp reduced 8 and black
bullhead 16 times. The abundance of roach decreased 11, rudd 10, pike-perch (Sander
lucioperca) 4 and freshwater bream (Abramis brama) 2 times. The abundances of
pumpkinseed increased 10, Monkey goby (Neogobius fluviatilis) 3 and topmouth gudgeon
2.5 times. The results showed that the fyke nets can be most effectively and selectively
applied to control the abundance of black bullhead, because simplicity and nonharmfulness
to other species. It is not possible to completely remove this species from the
ecosystem, but reduce the negative impact by suppression (removal programs) and
containment of existing state is crucial.
PB  - Institute of Biodiversity and Ecosystem Research Bulgarian Academy of Sciences
PB  - East and South European Network for Invasive Alien Species (ESENIAS)
PB  - Danube Region Invasive Alien Species Network (DIAS)
C3  - Joint ESENIAS and DIAS Scientific Conference and 12th ESENIAS Workshop
T1  - Results of selective removal of the black bullhead (Ameiurus melas) in two different lentic system
EP  - 126
SP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2371
ER  - 

@conference{
author = "Jaćimović, Milica and Nikolić, Dušan and Cvijanović, Gorčin and Višnjić Jeftić, Željka and Skorić, Stefan and Smederevac-Lalić, Marija",
year = "2023",
abstract = "In the sake of better understanding of the mechanisms that drive interactions between
invasive species and their ecosystems, results of selective removal of the black bullhead
(Ameiurus melas) in two different lentic systems were compared. Ponjavica Nature Park has
characteristical watercourse of the plains with very slow flow and the coastal remains of
wetlands. Markovačko reservoir was built for the needs of the irrigation system of the
nearby agricultural land. Both systems suffer dominance of the black bullhead,
characterized as a species of "globally high risk". Combining results of the mass removal,
the effects on the native fish populations have been empirically demonstrated and
quantified. Black bullhead selective removal in Ponjavica lasted from August - October
2018, April - September 2019, with 20,145 black bullhead individuals removed. It showed
decreased abundance of bleak (Alburnus alburnus) 2.3 times, while the abundances of
roach (Rutilus rutilus) and European perch (Perca fluviatilis) remained almost unchanged. A
significant increase in abundance was recorded in rudd (Scardinius erythrophthalmus) (5.3
times). The abundances of topmouth gudgeon (Pseudorasbora parva), pumpkinseed
(Lepomis gibbosus), and Prussian carp (Carassius gibelio) increased 25.5, 4.9, and 4.2 times,
respectively. The mass removal of black bullhead on Markovačko Lake lasted from August -
October 2020, April - September 2021, and 15,921 black bullhead specimens were
removed. When the post removal state from Markovačko lake was determined, two species
appeared in the sample for the first time: European perch and chub (Squalius cephalus). The
abundance of bleak increased 3 times, abundance of Prussian carp reduced 8 and black
bullhead 16 times. The abundance of roach decreased 11, rudd 10, pike-perch (Sander
lucioperca) 4 and freshwater bream (Abramis brama) 2 times. The abundances of
pumpkinseed increased 10, Monkey goby (Neogobius fluviatilis) 3 and topmouth gudgeon
2.5 times. The results showed that the fyke nets can be most effectively and selectively
applied to control the abundance of black bullhead, because simplicity and nonharmfulness
to other species. It is not possible to completely remove this species from the
ecosystem, but reduce the negative impact by suppression (removal programs) and
containment of existing state is crucial.",
publisher = "Institute of Biodiversity and Ecosystem Research Bulgarian Academy of Sciences, East and South European Network for Invasive Alien Species (ESENIAS), Danube Region Invasive Alien Species Network (DIAS)",
journal = "Joint ESENIAS and DIAS Scientific Conference and 12th ESENIAS Workshop",
title = "Results of selective removal of the black bullhead (Ameiurus melas) in two different lentic system",
pages = "126-126",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2371"
}

Jaćimović, M., Nikolić, D., Cvijanović, G., Višnjić Jeftić, Ž., Skorić, S.,& Smederevac-Lalić, M.. (2023). Results of selective removal of the black bullhead (Ameiurus melas) in two different lentic system. in Joint ESENIAS and DIAS Scientific Conference and 12th ESENIAS Workshop
Institute of Biodiversity and Ecosystem Research Bulgarian Academy of Sciences., 126-126.
https://hdl.handle.net/21.15107/rcub_rimsi_2371

Jaćimović M, Nikolić D, Cvijanović G, Višnjić Jeftić Ž, Skorić S, Smederevac-Lalić M. Results of selective removal of the black bullhead (Ameiurus melas) in two different lentic system. in Joint ESENIAS and DIAS Scientific Conference and 12th ESENIAS Workshop. 2023;:126-126.
https://hdl.handle.net/21.15107/rcub_rimsi_2371 .

Jaćimović, Milica, Nikolić, Dušan, Cvijanović, Gorčin, Višnjić Jeftić, Željka, Skorić, Stefan, Smederevac-Lalić, Marija, "Results of selective removal of the black bullhead (Ameiurus melas) in two different lentic system" in Joint ESENIAS and DIAS Scientific Conference and 12th ESENIAS Workshop (2023):126-126,
https://hdl.handle.net/21.15107/rcub_rimsi_2371 .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Vuković-Gačić, Branka
AU  - Lenhardt, Mirjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2216
AB  - In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems.
PB  - Springer Nature
T2  - Springer Nature, Ecotoxicology 32, 583–597 (2023)
T1  - Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia
EP  - 597
IS  - 32
SP  - 583
DO  - https://doi.org/10.1007/s10646-023-02663-6
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Sunjog, Karolina and Subotić, Srđan and Višnjić Jeftić, Željka and Raskovic, Bozidar and Poleksic, Vesna and Vuković-Gačić, Branka and Lenhardt, Mirjana",
year = "2023",
abstract = "In this study a battery of bioassays, both in vivo (metals and metalloids concentrations, erythrocyte morphometry, comet assay, micronucleus assay, and histopathological analyses) on vimba bream Vimba vimba (L., 1758) and white bream Blicca bjoerkna (L., 1758), and in vitro (treatment of HepG2 cells with native water samples) was applied to assess the harmful potential of untreated wastewater. Faecal indicator bacteria were quantified to assess the microbiological water quality. Vimba bream had significantly higher Fe concentrations in both liver and muscle, while white bream had higher Ca and Cu concentrations in liver. Vimba bream had a significantly higher level of DNA damage in both liver and blood cells, in comparison to white bream. Low levels of micronucleus and nuclear abnormalities were observed in both species. Erythrocytes morphometry did not show significant interspecific differences. Histopathological analyses revealed a similar response of the studied species, with a significantly higher presence of ceroid pigments in the liver of vimba bream. Treatment of HepG2 cells revealed the high genotoxic potential of water downstream of the discharge point. The results of this study clearly demonstrate the importance of effect-based monitoring, in order to enforce more efficient management of natural resources and implementation of wastewater treatment systems.",
publisher = "Springer Nature",
journal = "Springer Nature, Ecotoxicology 32, 583–597 (2023)",
title = "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia",
pages = "597-583",
number = "32",
doi = "https://doi.org/10.1007/s10646-023-02663-6"
}

Kostić-Vuković, J., Kolarević, S., Sunjog, K., Subotić, S., Višnjić Jeftić, Ž., Raskovic, B., Poleksic, V., Vuković-Gačić, B.,& Lenhardt, M.. (2023). Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Springer Nature, Ecotoxicology 32, 583–597 (2023)
Springer Nature.(32), 583-597.
https://doi.org/https://doi.org/10.1007/s10646-023-02663-6

Kostić-Vuković J, Kolarević S, Sunjog K, Subotić S, Višnjić Jeftić Ž, Raskovic B, Poleksic V, Vuković-Gačić B, Lenhardt M. Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia. in Springer Nature, Ecotoxicology 32, 583–597 (2023). 2023;(32):583-597.
doi:https://doi.org/10.1007/s10646-023-02663-6 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Sunjog, Karolina, Subotić, Srđan, Višnjić Jeftić, Željka, Raskovic, Bozidar, Poleksic, Vesna, Vuković-Gačić, Branka, Lenhardt, Mirjana, "Combined use of biomarkers to assess the impact of untreated wastewater from the Danube River, Serbia" in Springer Nature, Ecotoxicology 32, 583–597 (2023), no. 32 (2023):583-597,
https://doi.org/https://doi.org/10.1007/s10646-023-02663-6 . .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Đorđević Aleksić, Jelena
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2173
AB  - One of the most severe threats to the Danube River course through Serbia is the direct discharge of untreated wastewater. In biomonitoring studies, faecal indicator bacteria are used to reveal the presence of untreated communal wastewater, while biomarker response in indigenous biota serves as a useful indicator of their harmful potential. The objective of this study was to estimate the genotoxic potential of untreated communal wastewater at two sites on the Danube River, Višnjica (Belgrade) and Novi Banovci, by using in situ approach in white bream (Blicca bjoerkna L.). A comet assay was selected to measure DNA damage in erythrocytes, liver, and gill cells, while the micronucleus test was applied to measure chromosomal aberrations in erythrocytes.
Additionally, the accumulation of 22 elements in fish liver and muscle was analysed by ICP-OES meth- od. Simultaneous detection of total coliforms and E.coli in water was performed by enzyme-based Colilert-18 test (IDEXX). Comet assay revealed the highest level of DNA damage in gills at the site Novi Banovci, and in blood at the site Višnjica. The overall frequency of micronucleus at both sites was low. At both localities, the majority of analysed elements showed higher levels in the liver in comparison to muscle. Microbiological indicators confirmed the poor water quality, since at both sites water was categorised as critically to strongly polluted. The results of this study highlighted the importance of in situ biomonitoring approach and the need for more effective management of natural resources and implementation of wastewater treatment systems.
PB  - Serbian Society of Toxicology
C3  - 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia
T1  - Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_2173
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Sunjog, Karolina and Kolarević, Stoimir and Višnjić Jeftić, Željka and Subotić, Srđan and Đorđević Aleksić, Jelena and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2023",
abstract = "One of the most severe threats to the Danube River course through Serbia is the direct discharge of untreated wastewater. In biomonitoring studies, faecal indicator bacteria are used to reveal the presence of untreated communal wastewater, while biomarker response in indigenous biota serves as a useful indicator of their harmful potential. The objective of this study was to estimate the genotoxic potential of untreated communal wastewater at two sites on the Danube River, Višnjica (Belgrade) and Novi Banovci, by using in situ approach in white bream (Blicca bjoerkna L.). A comet assay was selected to measure DNA damage in erythrocytes, liver, and gill cells, while the micronucleus test was applied to measure chromosomal aberrations in erythrocytes.
Additionally, the accumulation of 22 elements in fish liver and muscle was analysed by ICP-OES meth- od. Simultaneous detection of total coliforms and E.coli in water was performed by enzyme-based Colilert-18 test (IDEXX). Comet assay revealed the highest level of DNA damage in gills at the site Novi Banovci, and in blood at the site Višnjica. The overall frequency of micronucleus at both sites was low. At both localities, the majority of analysed elements showed higher levels in the liver in comparison to muscle. Microbiological indicators confirmed the poor water quality, since at both sites water was categorised as critically to strongly polluted. The results of this study highlighted the importance of in situ biomonitoring approach and the need for more effective management of natural resources and implementation of wastewater treatment systems.",
publisher = "Serbian Society of Toxicology",
journal = "13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia",
title = "Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.",
pages = "72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_2173"
}

Kostić-Vuković, J., Sunjog, K., Kolarević, S., Višnjić Jeftić, Ž., Subotić, S., Đorđević Aleksić, J., Lenhardt, M.,& Vuković-Gačić, B.. (2023). Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.. in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia
Serbian Society of Toxicology., 72.
https://hdl.handle.net/21.15107/rcub_rimsi_2173

Kostić-Vuković J, Sunjog K, Kolarević S, Višnjić Jeftić Ž, Subotić S, Đorđević Aleksić J, Lenhardt M, Vuković-Gačić B. Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment.. in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia. 2023;:72.
https://hdl.handle.net/21.15107/rcub_rimsi_2173 .

Kostić-Vuković, Jovana, Sunjog, Karolina, Kolarević, Stoimir, Višnjić Jeftić, Željka, Subotić, Srđan, Đorđević Aleksić, Jelena, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Impact of wastewater effluents at two sites at Danube river: genotoxicological assessment." in 13. International Congress of the Serbian Society of Toxicology & 1. TOXSEE Regional Conference, Belgrade, Serbia (2023):72,
https://hdl.handle.net/21.15107/rcub_rimsi_2173 .

TY  - CONF
AU  - Jasnic, Nebojsa
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Lenhardt, Mirjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1746
AB  - The present study was carried out to investigate the differences in some enzymatic components of liver in vimba bream (Vimba vimba) and common nase (Chondrostoma nasus). For this purpose, 33 healthy vimba bream and 20 common nase were collected from commercial catches on the Danube River, near Belgrade (1172-1173 rkm), in 2016. The blood samples were taken and after separation of serum, the values of Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) enzymes activities were measured. Based on obtained results, the values of AST and ALT enzymes activities were slightly lower in the common nase compared to vimba bream, which is a possible indicator of better health and physiological status, and greater resistance to environmental pollutants present in the habitat. This difference was, however, not statistically confirmed. Considering that the selected fish were captured by the same method, having a similar ecology, living in similar habitats and under similar ecotoxicological conditions, it is not surprising that the differences in the activity of the measured liver enzymes were minimal. Since common nase is important bioindicator species, similar results could indicate that vimba bream could be considered as replacement species for this types of research, if future studies indicate the decline or endangerment of common nase populations.
PB  - Austrian Commitee Danube Research
C3  - 144th IAD Conference 2023, Krems, Austria
T1  - Comparative evaluation of liver enzymes activities in Vimba bream and Common nase living under the same ecotoxicological conditions
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1746
ER  - 

@conference{
author = "Jasnic, Nebojsa and Subotić, Srđan and Višnjić Jeftić, Željka and Lenhardt, Mirjana",
year = "2023",
abstract = "The present study was carried out to investigate the differences in some enzymatic components of liver in vimba bream (Vimba vimba) and common nase (Chondrostoma nasus). For this purpose, 33 healthy vimba bream and 20 common nase were collected from commercial catches on the Danube River, near Belgrade (1172-1173 rkm), in 2016. The blood samples were taken and after separation of serum, the values of Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) enzymes activities were measured. Based on obtained results, the values of AST and ALT enzymes activities were slightly lower in the common nase compared to vimba bream, which is a possible indicator of better health and physiological status, and greater resistance to environmental pollutants present in the habitat. This difference was, however, not statistically confirmed. Considering that the selected fish were captured by the same method, having a similar ecology, living in similar habitats and under similar ecotoxicological conditions, it is not surprising that the differences in the activity of the measured liver enzymes were minimal. Since common nase is important bioindicator species, similar results could indicate that vimba bream could be considered as replacement species for this types of research, if future studies indicate the decline or endangerment of common nase populations.",
publisher = "Austrian Commitee Danube Research",
journal = "144th IAD Conference 2023, Krems, Austria",
title = "Comparative evaluation of liver enzymes activities in Vimba bream and Common nase living under the same ecotoxicological conditions",
pages = "66",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1746"
}

Jasnic, N., Subotić, S., Višnjić Jeftić, Ž.,& Lenhardt, M.. (2023). Comparative evaluation of liver enzymes activities in Vimba bream and Common nase living under the same ecotoxicological conditions. in 144th IAD Conference 2023, Krems, Austria
Austrian Commitee Danube Research., 66.
https://hdl.handle.net/21.15107/rcub_rimsi_1746

Jasnic N, Subotić S, Višnjić Jeftić Ž, Lenhardt M. Comparative evaluation of liver enzymes activities in Vimba bream and Common nase living under the same ecotoxicological conditions. in 144th IAD Conference 2023, Krems, Austria. 2023;:66.
https://hdl.handle.net/21.15107/rcub_rimsi_1746 .

Jasnic, Nebojsa, Subotić, Srđan, Višnjić Jeftić, Željka, Lenhardt, Mirjana, "Comparative evaluation of liver enzymes activities in Vimba bream and Common nase living under the same ecotoxicological conditions" in 144th IAD Conference 2023, Krems, Austria (2023):66,
https://hdl.handle.net/21.15107/rcub_rimsi_1746 .

TY  - CONF
AU  - Višnjić Jeftić, Željka
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Subotić, Srđan
AU  - Lenhardt, Mirjana
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1745
AB  - Pontic shad (Alosa immaculata) is an anadromous species that lives in the Black Sea and Sea of Azov
and migrates into the Danube, Don and other rivers to spawn. It is still economically important fish
species in the Danube River. As the North-Western part of the Black Sea is heavily polluted
investigation was performed to determine heavy metal and element accumulation in muscle tissue of
Pontic shad as well as to record the level of histopathological changes. Pontic shad specimens were
caught on 863 river kilometer of the Danube and the first investigation was performed during 2007
and repeated one decade later in the period 2017-2019.
Element analysis was performed by inductively-coupled plasma – optic emission spectroscopy (ICPOES).
In both sampling periods, only concentrations of arsenic (As), copper (Cu), iron (Fe),
magnesium (Mg), strontium (Sr), and zinc (Zn) were above detection limit. Higher concentrations of
As, Cu, and Zn were detected in 2007, while higher concentrations of Fe, Mg, and Sr were measured
in 2019. Differences in concentrations of these elements, between sampling periods, were
statistically significant.
The gills of sampled fish were assessed using histopathology as a marker of general fish health state,
using semi-quantitative scoring system. The histopathological results revealed different pattern of
histopathological alterations in the gills of fish sampled during two distinct time periods. According to
the method used, there were no difference in total gill histopathological index, but alterations were
specific for fish sampled at two time points. Hyperemia and hyperplasia of respiratory epithelium
dominated in fish sampled at the year 2007, while necrosis of branchial tissue prevailed in fish
sampled a decade later.
PB  - Austrian Commitee Danube Research
C3  - 144th IAD Conference 2023, Krems, Austria
T1  - Difference in element accumulation and histopathology of Pontic shad (Alosa immaculata) migrants caught in the Danube River in interval of one decade
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1745
ER  - 

@conference{
author = "Višnjić Jeftić, Željka and Raskovic, Bozidar and Poleksic, Vesna and Subotić, Srđan and Lenhardt, Mirjana",
year = "2023",
abstract = "Pontic shad (Alosa immaculata) is an anadromous species that lives in the Black Sea and Sea of Azov
and migrates into the Danube, Don and other rivers to spawn. It is still economically important fish
species in the Danube River. As the North-Western part of the Black Sea is heavily polluted
investigation was performed to determine heavy metal and element accumulation in muscle tissue of
Pontic shad as well as to record the level of histopathological changes. Pontic shad specimens were
caught on 863 river kilometer of the Danube and the first investigation was performed during 2007
and repeated one decade later in the period 2017-2019.
Element analysis was performed by inductively-coupled plasma – optic emission spectroscopy (ICPOES).
In both sampling periods, only concentrations of arsenic (As), copper (Cu), iron (Fe),
magnesium (Mg), strontium (Sr), and zinc (Zn) were above detection limit. Higher concentrations of
As, Cu, and Zn were detected in 2007, while higher concentrations of Fe, Mg, and Sr were measured
in 2019. Differences in concentrations of these elements, between sampling periods, were
statistically significant.
The gills of sampled fish were assessed using histopathology as a marker of general fish health state,
using semi-quantitative scoring system. The histopathological results revealed different pattern of
histopathological alterations in the gills of fish sampled during two distinct time periods. According to
the method used, there were no difference in total gill histopathological index, but alterations were
specific for fish sampled at two time points. Hyperemia and hyperplasia of respiratory epithelium
dominated in fish sampled at the year 2007, while necrosis of branchial tissue prevailed in fish
sampled a decade later.",
publisher = "Austrian Commitee Danube Research",
journal = "144th IAD Conference 2023, Krems, Austria",
title = "Difference in element accumulation and histopathology of Pontic shad (Alosa immaculata) migrants caught in the Danube River in interval of one decade",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1745"
}

Višnjić Jeftić, Ž., Raskovic, B., Poleksic, V., Subotić, S.,& Lenhardt, M.. (2023). Difference in element accumulation and histopathology of Pontic shad (Alosa immaculata) migrants caught in the Danube River in interval of one decade. in 144th IAD Conference 2023, Krems, Austria
Austrian Commitee Danube Research., 33.
https://hdl.handle.net/21.15107/rcub_rimsi_1745

Višnjić Jeftić Ž, Raskovic B, Poleksic V, Subotić S, Lenhardt M. Difference in element accumulation and histopathology of Pontic shad (Alosa immaculata) migrants caught in the Danube River in interval of one decade. in 144th IAD Conference 2023, Krems, Austria. 2023;:33.
https://hdl.handle.net/21.15107/rcub_rimsi_1745 .

Višnjić Jeftić, Željka, Raskovic, Bozidar, Poleksic, Vesna, Subotić, Srđan, Lenhardt, Mirjana, "Difference in element accumulation and histopathology of Pontic shad (Alosa immaculata) migrants caught in the Danube River in interval of one decade" in 144th IAD Conference 2023, Krems, Austria (2023):33,
https://hdl.handle.net/21.15107/rcub_rimsi_1745 .

TY  - JOUR
AU  - Radonjić, Marina
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Mrdak, Danilo
AU  - Cirovic, Dusko
PY  - 2023
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/2622
AB  - The aim of this study was to determine the concentrations of 28 elements in the liver, kidney, muscle, and heart of a small Indian mongoose from Montenegro. Element concentrations were determined using inductively coupled plasma optical emission spectrometry (ICP-OES). Significant differences in elemental concentrations between analyzed tissues were observed for Ag, Al, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, P, S, Se, and Zn. The concentrations of Ag, Fe, Mo, and S were significantly different between all analyzed tissues. Muscle tissue is characterised by higher concentrations of some macroelements (K, Mg, S) and liver tissue by higher concentrations of some trace elements (Fe, Mn, Mo, and Zn). Cardiac tissue could be the target organ for bioaccumulation of Ag and Se. The molar ratio Se:Hg was higher than 1 in all studied tissues. There were few significant differences between element concentrations in mongooses from three analyzed (Airport, Saline, Inland) groups. Significantly higher concentrations of Ag and Se were found in liver tissue of Inland mongooses compared to animals from Airport and Saline. There were few significant differences (Zn in liver and muscle, Fe in muscle) in element concentrations between females and males.
PB  - Springer
T2  - Environmental Science and Pollution Research
T1  - Assessment of macro-, trace- and toxic elements in Small Indian mongoose, Herpestes auropunctatus (Hodgson, 1836), from Montenegro: potential use for biomonitoring
EP  - 60523
IS  - 21
SP  - 60514
VL  - 30
DO  - 10.1007/s11356-023-26885-7
ER  - 

@article{
author = "Radonjić, Marina and Subotić, Srđan and Višnjić Jeftić, Željka and Mrdak, Danilo and Cirovic, Dusko",
year = "2023",
abstract = "The aim of this study was to determine the concentrations of 28 elements in the liver, kidney, muscle, and heart of a small Indian mongoose from Montenegro. Element concentrations were determined using inductively coupled plasma optical emission spectrometry (ICP-OES). Significant differences in elemental concentrations between analyzed tissues were observed for Ag, Al, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, P, S, Se, and Zn. The concentrations of Ag, Fe, Mo, and S were significantly different between all analyzed tissues. Muscle tissue is characterised by higher concentrations of some macroelements (K, Mg, S) and liver tissue by higher concentrations of some trace elements (Fe, Mn, Mo, and Zn). Cardiac tissue could be the target organ for bioaccumulation of Ag and Se. The molar ratio Se:Hg was higher than 1 in all studied tissues. There were few significant differences between element concentrations in mongooses from three analyzed (Airport, Saline, Inland) groups. Significantly higher concentrations of Ag and Se were found in liver tissue of Inland mongooses compared to animals from Airport and Saline. There were few significant differences (Zn in liver and muscle, Fe in muscle) in element concentrations between females and males.",
publisher = "Springer",
journal = "Environmental Science and Pollution Research",
title = "Assessment of macro-, trace- and toxic elements in Small Indian mongoose, Herpestes auropunctatus (Hodgson, 1836), from Montenegro: potential use for biomonitoring",
pages = "60523-60514",
number = "21",
volume = "30",
doi = "10.1007/s11356-023-26885-7"
}

Radonjić, M., Subotić, S., Višnjić Jeftić, Ž., Mrdak, D.,& Cirovic, D.. (2023). Assessment of macro-, trace- and toxic elements in Small Indian mongoose, Herpestes auropunctatus (Hodgson, 1836), from Montenegro: potential use for biomonitoring. in Environmental Science and Pollution Research
Springer., 30(21), 60514-60523.
https://doi.org/10.1007/s11356-023-26885-7

Radonjić M, Subotić S, Višnjić Jeftić Ž, Mrdak D, Cirovic D. Assessment of macro-, trace- and toxic elements in Small Indian mongoose, Herpestes auropunctatus (Hodgson, 1836), from Montenegro: potential use for biomonitoring. in Environmental Science and Pollution Research. 2023;30(21):60514-60523.
doi:10.1007/s11356-023-26885-7 .

Radonjić, Marina, Subotić, Srđan, Višnjić Jeftić, Željka, Mrdak, Danilo, Cirovic, Dusko, "Assessment of macro-, trace- and toxic elements in Small Indian mongoose, Herpestes auropunctatus (Hodgson, 1836), from Montenegro: potential use for biomonitoring" in Environmental Science and Pollution Research, 30, no. 21 (2023):60514-60523,
https://doi.org/10.1007/s11356-023-26885-7 . .

TY  - CONF
AU  - Jaćimović, Milica
AU  - Smederevac-Lalić, Marija
AU  - Nikolić, Dušan
AU  - Cvijanović, Gorčin
AU  - Spasić, Slađana
AU  - Višnjić Jeftić, Željka
AU  - Skorić, Stefan
AU  - Krpo-Ćetković, Jasmina
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1709
AB  - North American black bullhead (Ameiurus melas) is an ictalurid species that has become
widespread outside of its native range, causing undesirable ecological impacts. Numerous
studies have suggested that its physical removal could be a way to reduce any potential
detrimental effects of this species. This study measured the effects on population dynamics of
both native and non-native fish species following the removal of 20,145 black bullheads from
the Ponjavica Nature Park. The most significant changes between the zero-point state and the
post-removal state have been recorded among the populations of non-native fish species: the
abundances of topmouth gudgeon (Pseudorasbora parva), pumpkinseed (Lepomis gibbosus),
and Prussian carp (Carassius gibelio) increased 25.5, 4.9, and 4.2 times, respectively.
Previous research has shown that smaller black bullhead individuals enter into competitive
relationships with the topmouth gudgeon, while larger individuals use the topmouth gudgeon
as prey. By removing black bullhead individuals from the ecosystem, a part of the ecological
niche used by both species was freed. Also, the predatory pressure on topmouth gudgeon
decreased by removing larger black bullhead individuals. All these factors led to an increase
in the abundance of topmouth gudgeon. Black bullhead and pumpkinseed have overlapping
diets and are therefore considered competitors for food. Moreover, research on the black
bullhead's feeding ecology showed that this species feeds on the pumpkinseed. When a
greater number of black bullhead individuals were removed from the ecosystem, it led to an
increase in the abundance of pumpkinseed. The abundance of Prussian carp also increased
after the removal of the black bullhead. The Prussian carp and the black bullhead are quite
similar in terms of diet habits, habitat preferences, and reproductive characteristics. Since
these two species are in direct competition, the decline in the black bullhead population
contributed to an increase in the Prussian carp population. These results point to the fact that
management programs must include multiple species and methods, and one of them is the
removal of all undesirable, invasive non-native species. Further research should also focus on
possible diet shifts in allochthonous fishes after removing the black bullhead.
PB  - General Directorate of Fisheries and Aquaculture, Ministry of Agriculture and Forestry, Türkiye (GDFA); Mediterranean Fisheries Research, Production and Training Institute, Ministry of Agriculture and Forestry, Türkiye (MFRPTI); Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences (IBER-BAS); East and South European Network for Invasive Alien Species (ESENIAS); Danube Region Invasive Alien Species Network (DIAS)
C3  - Joint ESENIAS and DIAS Scientific Conference 2022 and 11th ESENIAS Workshop ‘Invasive alien species under conditions of global crisis
T1  - Effects of selective removal of the black bullhead (Ameiurus melas) on other non-native fish populations in the Ponjavica Nature Park (Serbia)
EP  - 99
SP  - 99
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1709
ER  - 

@conference{
author = "Jaćimović, Milica and Smederevac-Lalić, Marija and Nikolić, Dušan and Cvijanović, Gorčin and Spasić, Slađana and Višnjić Jeftić, Željka and Skorić, Stefan and Krpo-Ćetković, Jasmina",
year = "2022",
abstract = "North American black bullhead (Ameiurus melas) is an ictalurid species that has become
widespread outside of its native range, causing undesirable ecological impacts. Numerous
studies have suggested that its physical removal could be a way to reduce any potential
detrimental effects of this species. This study measured the effects on population dynamics of
both native and non-native fish species following the removal of 20,145 black bullheads from
the Ponjavica Nature Park. The most significant changes between the zero-point state and the
post-removal state have been recorded among the populations of non-native fish species: the
abundances of topmouth gudgeon (Pseudorasbora parva), pumpkinseed (Lepomis gibbosus),
and Prussian carp (Carassius gibelio) increased 25.5, 4.9, and 4.2 times, respectively.
Previous research has shown that smaller black bullhead individuals enter into competitive
relationships with the topmouth gudgeon, while larger individuals use the topmouth gudgeon
as prey. By removing black bullhead individuals from the ecosystem, a part of the ecological
niche used by both species was freed. Also, the predatory pressure on topmouth gudgeon
decreased by removing larger black bullhead individuals. All these factors led to an increase
in the abundance of topmouth gudgeon. Black bullhead and pumpkinseed have overlapping
diets and are therefore considered competitors for food. Moreover, research on the black
bullhead's feeding ecology showed that this species feeds on the pumpkinseed. When a
greater number of black bullhead individuals were removed from the ecosystem, it led to an
increase in the abundance of pumpkinseed. The abundance of Prussian carp also increased
after the removal of the black bullhead. The Prussian carp and the black bullhead are quite
similar in terms of diet habits, habitat preferences, and reproductive characteristics. Since
these two species are in direct competition, the decline in the black bullhead population
contributed to an increase in the Prussian carp population. These results point to the fact that
management programs must include multiple species and methods, and one of them is the
removal of all undesirable, invasive non-native species. Further research should also focus on
possible diet shifts in allochthonous fishes after removing the black bullhead.",
publisher = "General Directorate of Fisheries and Aquaculture, Ministry of Agriculture and Forestry, Türkiye (GDFA); Mediterranean Fisheries Research, Production and Training Institute, Ministry of Agriculture and Forestry, Türkiye (MFRPTI); Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences (IBER-BAS); East and South European Network for Invasive Alien Species (ESENIAS); Danube Region Invasive Alien Species Network (DIAS)",
journal = "Joint ESENIAS and DIAS Scientific Conference 2022 and 11th ESENIAS Workshop ‘Invasive alien species under conditions of global crisis",
title = "Effects of selective removal of the black bullhead (Ameiurus melas) on other non-native fish populations in the Ponjavica Nature Park (Serbia)",
pages = "99-99",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1709"
}

Jaćimović, M., Smederevac-Lalić, M., Nikolić, D., Cvijanović, G., Spasić, S., Višnjić Jeftić, Ž., Skorić, S.,& Krpo-Ćetković, J.. (2022). Effects of selective removal of the black bullhead (Ameiurus melas) on other non-native fish populations in the Ponjavica Nature Park (Serbia). in Joint ESENIAS and DIAS Scientific Conference 2022 and 11th ESENIAS Workshop ‘Invasive alien species under conditions of global crisis
General Directorate of Fisheries and Aquaculture, Ministry of Agriculture and Forestry, Türkiye (GDFA); Mediterranean Fisheries Research, Production and Training Institute, Ministry of Agriculture and Forestry, Türkiye (MFRPTI); Institute of Biodiversity and Ecosystem Research, Bulgarian Academy of Sciences (IBER-BAS); East and South European Network for Invasive Alien Species (ESENIAS); Danube Region Invasive Alien Species Network (DIAS)., 99-99.
https://hdl.handle.net/21.15107/rcub_rimsi_1709

Jaćimović M, Smederevac-Lalić M, Nikolić D, Cvijanović G, Spasić S, Višnjić Jeftić Ž, Skorić S, Krpo-Ćetković J. Effects of selective removal of the black bullhead (Ameiurus melas) on other non-native fish populations in the Ponjavica Nature Park (Serbia). in Joint ESENIAS and DIAS Scientific Conference 2022 and 11th ESENIAS Workshop ‘Invasive alien species under conditions of global crisis. 2022;:99-99.
https://hdl.handle.net/21.15107/rcub_rimsi_1709 .

Jaćimović, Milica, Smederevac-Lalić, Marija, Nikolić, Dušan, Cvijanović, Gorčin, Spasić, Slađana, Višnjić Jeftić, Željka, Skorić, Stefan, Krpo-Ćetković, Jasmina, "Effects of selective removal of the black bullhead (Ameiurus melas) on other non-native fish populations in the Ponjavica Nature Park (Serbia)" in Joint ESENIAS and DIAS Scientific Conference 2022 and 11th ESENIAS Workshop ‘Invasive alien species under conditions of global crisis (2022):99-99,
https://hdl.handle.net/21.15107/rcub_rimsi_1709 .

TY  - CONF
AU  - Jaćimović, Milica
AU  - Smederevac-Lalić, Marija
AU  - Nikolić, Dušan
AU  - Cvijanović, Gorčin
AU  - Spasić, Slađana
AU  - Višnjić Jeftić, Željka
AU  - Skorić, Stefan
AU  - Krpo-Ćetković, Jasmina
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1687
AB  - Црни амерички патуљасти сом (Ameiurus melas) представља једну од најзаступљенијих и најуспешнијих инвазивних врста риба која насељава копнене воде Европе, укључујући и Србију. У досадашњим истраживањима напомиње се да би санациони излов могао да буде потенцијално решење проблема које ова врста изазива у водама у којима је интродукован. У овом истраживању, реализованом од јуна 2018. до октобра 2019. године, квантификована је популациона динамика аутохтоних и алохтоних врста риба у Парку природе „Поњавица“ након селективног излова црног америчког патуљастог сома. Укупно је изловљено 20.145 јединки ове врсте што је утицало на абунданцу и аутохтоних и алохтоних врста риба. Статистички значајно повећање абунданце забележено је код црвенперке (Scardinius erythrophthalmus) (5,3 пута), док се абунданца уклије (Alburnus alburnus) смањила 2,3 пута. Најзначајнија промена забележена је међу популацијама алохтоних врста риба. Абунданца амурског чебачока (Pseudorasbora parva) повећана је 25,5 пута, сунчице (Lepomis gibbosus) 4,9 пута и бабушке (Carassius gibelio) 4,2 пута. Вредност Shannon-овог индекса диверзитета (H)1 била је двоструко већа након ове интервенције, док се вредност Simpson-овог индекса доминантности (C)2 смањила три пута. Вредности улова по јединици напора (енгл. catch-per-unit-effort – CPUE) и биомасе по јединици напора (енгл. biomass-per-unit-effort – BPUE) свих узоркованих врста током селективног излова показују доминацију црног америчког патуљастог сома. Резултати указују на то да је селективни излов ове врсте имао како позитивне, тако и негативне последице на насеље риба на истраживаном подручју.
PB  - Српско биолошко друштво
C3  - ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ
T1  - Утицај селективног излова црног америчког патуљастог сома (Ameiurus melas) на насеље риба у Парку природе „Поњавица“
EP  - 161
SP  - 161
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1687
ER  - 

@conference{
author = "Jaćimović, Milica and Smederevac-Lalić, Marija and Nikolić, Dušan and Cvijanović, Gorčin and Spasić, Slađana and Višnjić Jeftić, Željka and Skorić, Stefan and Krpo-Ćetković, Jasmina",
year = "2022",
abstract = "Црни амерички патуљасти сом (Ameiurus melas) представља једну од најзаступљенијих и најуспешнијих инвазивних врста риба која насељава копнене воде Европе, укључујући и Србију. У досадашњим истраживањима напомиње се да би санациони излов могао да буде потенцијално решење проблема које ова врста изазива у водама у којима је интродукован. У овом истраживању, реализованом од јуна 2018. до октобра 2019. године, квантификована је популациона динамика аутохтоних и алохтоних врста риба у Парку природе „Поњавица“ након селективног излова црног америчког патуљастог сома. Укупно је изловљено 20.145 јединки ове врсте што је утицало на абунданцу и аутохтоних и алохтоних врста риба. Статистички значајно повећање абунданце забележено је код црвенперке (Scardinius erythrophthalmus) (5,3 пута), док се абунданца уклије (Alburnus alburnus) смањила 2,3 пута. Најзначајнија промена забележена је међу популацијама алохтоних врста риба. Абунданца амурског чебачока (Pseudorasbora parva) повећана је 25,5 пута, сунчице (Lepomis gibbosus) 4,9 пута и бабушке (Carassius gibelio) 4,2 пута. Вредност Shannon-овог индекса диверзитета (H)1 била је двоструко већа након ове интервенције, док се вредност Simpson-овог индекса доминантности (C)2 смањила три пута. Вредности улова по јединици напора (енгл. catch-per-unit-effort – CPUE) и биомасе по јединици напора (енгл. biomass-per-unit-effort – BPUE) свих узоркованих врста током селективног излова показују доминацију црног америчког патуљастог сома. Резултати указују на то да је селективни излов ове врсте имао како позитивне, тако и негативне последице на насеље риба на истраживаном подручју.",
publisher = "Српско биолошко друштво",
journal = "ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ",
title = "Утицај селективног излова црног америчког патуљастог сома (Ameiurus melas) на насеље риба у Парку природе „Поњавица“",
pages = "161-161",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1687"
}

Jaćimović, M., Smederevac-Lalić, M., Nikolić, D., Cvijanović, G., Spasić, S., Višnjić Jeftić, Ž., Skorić, S.,& Krpo-Ćetković, J.. (2022). Утицај селективног излова црног америчког патуљастог сома (Ameiurus melas) на насеље риба у Парку природе „Поњавица“. in ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ
Српско биолошко друштво., 161-161.
https://hdl.handle.net/21.15107/rcub_rimsi_1687

Jaćimović M, Smederevac-Lalić M, Nikolić D, Cvijanović G, Spasić S, Višnjić Jeftić Ž, Skorić S, Krpo-Ćetković J. Утицај селективног излова црног америчког патуљастог сома (Ameiurus melas) на насеље риба у Парку природе „Поњавица“. in ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ. 2022;:161-161.
https://hdl.handle.net/21.15107/rcub_rimsi_1687 .

Jaćimović, Milica, Smederevac-Lalić, Marija, Nikolić, Dušan, Cvijanović, Gorčin, Spasić, Slađana, Višnjić Jeftić, Željka, Skorić, Stefan, Krpo-Ćetković, Jasmina, "Утицај селективног излова црног америчког патуљастог сома (Ameiurus melas) на насеље риба у Парку природе „Поњавица“" in ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ (2022):161-161,
https://hdl.handle.net/21.15107/rcub_rimsi_1687 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Vuković-Gačić, Branka
AU  - Lenhardt, Mirjana
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1752
AB  - Непречишћене комуналне и индустријске отпадне воде представљају извор 
хемијског и микробиолошког загађења водених екосистема и угрожавају све 
видове њиховог коришћења. Ово истраживање је спроведено у јулу 2017. године, 
на реци Дунав, локалитету Нови Бановци, који је изложен испусту колектора 
непречишћених индустријских и комуналних отпадних вода насеља са око 40 000 
становника. Вода је узоркована за микробиолошку анализу индикатора фекалног 
загађења ензимском методом Colilert (IDEXX). Неколико десетина метара од 
испуста колектора узорковано је 5 јединки шљивара Vimba vimba (L., 1758) за 
анализу метала у мишићу и јетри методом ICP-OES и нивоа ДНК оштећења у 
ћелијама крви, јетре и шкрга применом алкалног комет теста. Према нашим 
сазнањима ово је прва студија у којој је комет тест примењен на ћелијама 
шљивара. На основу индикатора фекалног загађења (укупни колиформи и E. coli) 
вода се могла класификовати као критично загађена (III класа). Највиши ниво ДНК 
оштећења забележен је у ћелијама крви, потом следе шкрге и јетра. Ниво ДНК 
оштећења у ћелијама крви био је статистички значајно виши у односу на јетру. На 
основу концентрација Fe и Zn, за које су прописане максимално дозвољене 
концентрације за рибље производе у лименој амбалажи, месо шљивара је безбедно 
за исхрану људи.
PB  - Српско биолошко друштво
C3  - ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ
T1  - Nivo DNК oštećenja i akumulacije metala u jedinkama Vimba vimba (L., 1758) izloženim neprečišćenim otpadnim vodama u reci Dunav, Srbija.
SP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1752
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Sunjog, Karolina and Višnjić Jeftić, Željka and Subotić, Srđan and Vuković-Gačić, Branka and Lenhardt, Mirjana",
year = "2022",
abstract = "Непречишћене комуналне и индустријске отпадне воде представљају извор 
хемијског и микробиолошког загађења водених екосистема и угрожавају све 
видове њиховог коришћења. Ово истраживање је спроведено у јулу 2017. године, 
на реци Дунав, локалитету Нови Бановци, који је изложен испусту колектора 
непречишћених индустријских и комуналних отпадних вода насеља са око 40 000 
становника. Вода је узоркована за микробиолошку анализу индикатора фекалног 
загађења ензимском методом Colilert (IDEXX). Неколико десетина метара од 
испуста колектора узорковано је 5 јединки шљивара Vimba vimba (L., 1758) за 
анализу метала у мишићу и јетри методом ICP-OES и нивоа ДНК оштећења у 
ћелијама крви, јетре и шкрга применом алкалног комет теста. Према нашим 
сазнањима ово је прва студија у којој је комет тест примењен на ћелијама 
шљивара. На основу индикатора фекалног загађења (укупни колиформи и E. coli) 
вода се могла класификовати као критично загађена (III класа). Највиши ниво ДНК 
оштећења забележен је у ћелијама крви, потом следе шкрге и јетра. Ниво ДНК 
оштећења у ћелијама крви био је статистички значајно виши у односу на јетру. На 
основу концентрација Fe и Zn, за које су прописане максимално дозвољене 
концентрације за рибље производе у лименој амбалажи, месо шљивара је безбедно 
за исхрану људи.",
publisher = "Српско биолошко друштво",
journal = "ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ",
title = "Nivo DNК oštećenja i akumulacije metala u jedinkama Vimba vimba (L., 1758) izloženim neprečišćenim otpadnim vodama u reci Dunav, Srbija.",
pages = "156",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1752"
}

Kostić-Vuković, J., Kolarević, S., Sunjog, K., Višnjić Jeftić, Ž., Subotić, S., Vuković-Gačić, B.,& Lenhardt, M.. (2022). Nivo DNК oštećenja i akumulacije metala u jedinkama Vimba vimba (L., 1758) izloženim neprečišćenim otpadnim vodama u reci Dunav, Srbija.. in ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ
Српско биолошко друштво., 156.
https://hdl.handle.net/21.15107/rcub_rimsi_1752

Kostić-Vuković J, Kolarević S, Sunjog K, Višnjić Jeftić Ž, Subotić S, Vuković-Gačić B, Lenhardt M. Nivo DNК oštećenja i akumulacije metala u jedinkama Vimba vimba (L., 1758) izloženim neprečišćenim otpadnim vodama u reci Dunav, Srbija.. in ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ. 2022;:156.
https://hdl.handle.net/21.15107/rcub_rimsi_1752 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Sunjog, Karolina, Višnjić Jeftić, Željka, Subotić, Srđan, Vuković-Gačić, Branka, Lenhardt, Mirjana, "Nivo DNК oštećenja i akumulacije metala u jedinkama Vimba vimba (L., 1758) izloženim neprečišćenim otpadnim vodama u reci Dunav, Srbija." in ТРЕЋИ КОНГРЕС БИОЛОГА СРБИЈЕ (2022):156,
https://hdl.handle.net/21.15107/rcub_rimsi_1752 .

TY  - CONF
AU  - Đorđević Aleksić, Jelena
AU  - Kostić-Vuković, Jovana
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Subotić, Srđan
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1688
AB  - Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly 
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem 
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the 
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated 
municipal and industrial waste waters. 
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver 
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was 
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet 
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to 
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the 
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored 
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay 
on HepG2 cells showed significant difference between values of negative control samples and samples 
of water from effluent site and downstream of wastewater discharge. There was no significant 
difference between negative control and upstream samples of water. 
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem. 
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More 
importantly, because Serbia still doesn’t have waste water treatment plants, this kind of research 
points out the imperative for implementation of this facilities.
PB  - Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany
C3  - 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin
T1  - Genotoxicity assessment of Danube River: in situ and in vitro methods.
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1688
ER  - 

@conference{
author = "Đorđević Aleksić, Jelena and Kostić-Vuković, Jovana and Sunjog, Karolina and Višnjić Jeftić, Željka and Subotić, Srđan and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2021",
abstract = "Water quality in the Danube River Basin is largely influenced by the inputs of pollutants - particularly 
excessive nutrients, organic material, and hazardous substances, which affects the whole ecosystem 
at various levels. The major goal of this study was to evaluate the level of genotoxic potential at the 
site at Danube River, Novi Banovci, which is under significant anthropogenic pressure of untreated 
municipal and industrial waste waters. 
DNA damage in situ was assessed in 5 specimens of white bream (Blicca bjoerkna) erythrocytes, liver 
and gills cells with comet assay and in erythrocytes with micronucleus test. Additionally, genotoxicity
of native water samples collected from the site and upstream and downstream of the site was 
evaluated by the comet assay on HepG2 cells. Microscopic images of comets were scored using Comet 
IV Computer Software (Perceptive Instruments, UK) and tail intensity was chosen as parameters to 
assess the DNA damage.
Comet assay on white bream showed that gills were the most affected tissue, while liver showed the 
smallest values. Micronuclei frequency was low in general, with mean of 1 micronuclei in 5000 scored 
erythrocytes, with no significant correlation to the values of comet assay in erythrocytes. Comet assay 
on HepG2 cells showed significant difference between values of negative control samples and samples 
of water from effluent site and downstream of wastewater discharge. There was no significant 
difference between negative control and upstream samples of water. 
Our results showed that great impact of municipal and industrial wastewaters on the river ecosystem. 
The application of in vitro and in situ tests gives a better insight into genotoxic effects. More 
importantly, because Serbia still doesn’t have waste water treatment plants, this kind of research 
points out the imperative for implementation of this facilities.",
publisher = "Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany",
journal = "43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin",
title = "Genotoxicity assessment of Danube River: in situ and in vitro methods.",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1688"
}

Đorđević Aleksić, J., Kostić-Vuković, J., Sunjog, K., Višnjić Jeftić, Ž., Subotić, S., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2021). Genotoxicity assessment of Danube River: in situ and in vitro methods.. in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin
Aueninstitut Neuburg/Donau Schloss Grünau 86633 Neuburg/Donau Germany..
https://hdl.handle.net/21.15107/rcub_rimsi_1688

Đorđević Aleksić J, Kostić-Vuković J, Sunjog K, Višnjić Jeftić Ž, Subotić S, Gačić Z, Lenhardt M, Vuković-Gačić B. Genotoxicity assessment of Danube River: in situ and in vitro methods.. in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin. 2021;.
https://hdl.handle.net/21.15107/rcub_rimsi_1688 .

Đorđević Aleksić, Jelena, Kostić-Vuković, Jovana, Sunjog, Karolina, Višnjić Jeftić, Željka, Subotić, Srđan, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Genotoxicity assessment of Danube River: in situ and in vitro methods." in 43rd IAD Conference, Rivers and Floodplains in the Anthropocene: Upcoming Challenges in the Danube River Basin (2021),
https://hdl.handle.net/21.15107/rcub_rimsi_1688 .

TY  - JOUR
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Višnjić Jeftić, Željka
AU  - Rasković, Božidar
AU  - Poleksic, Vesna
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1501
AB  - This study was conducted on the Danube River locality Visnjica, exposed to the discharge of the largest wastewater collector in Serbia's capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.
PB  - Springer, Dordrecht
T2  - Environmental Monitoring and Assessment
T1  - Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia
IS  - 8
VL  - 193
DO  - 10.1007/s10661-021-09232-6
ER  - 

@article{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Višnjić Jeftić, Željka and Rasković, Božidar and Poleksic, Vesna and Gačić, Zoran and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2021",
abstract = "This study was conducted on the Danube River locality Visnjica, exposed to the discharge of the largest wastewater collector in Serbia's capital, Belgrade. Concentrations of metals and metalloids (Al, Fe, Cr, As, Sr, Mn, Cd, Zn, Mo, Cu, Li, Ni, B, Co, Pb, and Ba) and histopathological alterations were investigated in different tissues of common bream during one representative month of each season in 2014. This is the first study in which these two biomarkers were assessed parallelly in common bream. The highest concentrations of examined elements were noticed in gills and the lowest in muscle. Statistically significant differences in element concentrations between different seasons were noticed only in gills for Al, Cu, and Fe. Concentrations of As and Pb in fish muscle were below the maximum acceptable concentrations (MAC). The histopathological index (HI) of the liver showed higher values in comparison to the HI of the gills. Histopathological index of the gills had a significantly higher score in November in comparison to August. The liver HI had the highest score in April, and the lowest in August, while the total histopathological index had the highest score in November, and the lowest in August, both without significant differences between the months. This study endorses gills and liver as reliable organs for studying accumulation and histopathology as biomarkers of environmental changes. A faster reaction of the gills was confirmed since seasonal variations of both biomarkers were observed in this organ. Common bream proved as a good indicator of the state of organisms in polluted environments.",
publisher = "Springer, Dordrecht",
journal = "Environmental Monitoring and Assessment",
title = "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia",
number = "8",
volume = "193",
doi = "10.1007/s10661-021-09232-6"
}

Kostić-Vuković, J., Kolarević, S., Kracun-Kolarević, M., Višnjić Jeftić, Ž., Rasković, B., Poleksic, V., Gačić, Z., Lenhardt, M.,& Vukovic-Gacic, B.. (2021). Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment
Springer, Dordrecht., 193(8).
https://doi.org/10.1007/s10661-021-09232-6

Kostić-Vuković J, Kolarević S, Kracun-Kolarević M, Višnjić Jeftić Ž, Rasković B, Poleksic V, Gačić Z, Lenhardt M, Vukovic-Gacic B. Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia. in Environmental Monitoring and Assessment. 2021;193(8).
doi:10.1007/s10661-021-09232-6 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Višnjić Jeftić, Željka, Rasković, Božidar, Poleksic, Vesna, Gačić, Zoran, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipal wastewater in the Danube River in Belgrade, Serbia" in Environmental Monitoring and Assessment, 193, no. 8 (2021),
https://doi.org/10.1007/s10661-021-09232-6 . .

TY  - JOUR
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Bojović, Srdan
AU  - Đikanović, Vesna
AU  - Krpo-Cetković, Jasmina
AU  - Lenhardt, Mirjana
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1464
AB  - Vimba bream (Vimba vimba) individuals were collected from the Danube River near Belgrade, from May 2016 to May 2017. Samples of muscle and liver tissue were analyzed for concentrations of 25 elements (macro-, micro-, and toxic elements) using inductively coupled plasma optical emission spectrometry (ICP-OES). For both tissues, the highest concentrations of most elements were observed in late spring/early summer (months of May and June). Majority of statistically significant correlations between elements were positive in both tissues. Contributions of Ca, K, and Mg to human diet were in a similar range. The lowest contribution of all macroelements was observed for Na, while the contribution of Se was well above the recommended dietary allowance for this element. Levels of detected elements were well below MAC values prescribed by national and international regulations. The highest value of Hg liver/muscle index (>1) was observed in August, which could indicate a higher contamination of the sampling location in that month. Se:Hg molar ratio never drops below 1, which could indicate that Hg toxicity is prevented. THQ values showed little variation thought the year and were well below the threshold of 1, with the exception of THQ for Al in May (0.92). This element also strongly affects the hazard index.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Seasonal variations of macro-, micro-, and toxic elements in tissues of vimba bream (Vimba vimba) from the Danube River near Belgrade, Serbia
EP  - 63101
IS  - 44
SP  - 63087
VL  - 28
DO  - 10.1007/s11356-021-15073-0
ER  - 

@article{
author = "Subotić, Srđan and Višnjić Jeftić, Željka and Bojović, Srdan and Đikanović, Vesna and Krpo-Cetković, Jasmina and Lenhardt, Mirjana",
year = "2021",
abstract = "Vimba bream (Vimba vimba) individuals were collected from the Danube River near Belgrade, from May 2016 to May 2017. Samples of muscle and liver tissue were analyzed for concentrations of 25 elements (macro-, micro-, and toxic elements) using inductively coupled plasma optical emission spectrometry (ICP-OES). For both tissues, the highest concentrations of most elements were observed in late spring/early summer (months of May and June). Majority of statistically significant correlations between elements were positive in both tissues. Contributions of Ca, K, and Mg to human diet were in a similar range. The lowest contribution of all macroelements was observed for Na, while the contribution of Se was well above the recommended dietary allowance for this element. Levels of detected elements were well below MAC values prescribed by national and international regulations. The highest value of Hg liver/muscle index (>1) was observed in August, which could indicate a higher contamination of the sampling location in that month. Se:Hg molar ratio never drops below 1, which could indicate that Hg toxicity is prevented. THQ values showed little variation thought the year and were well below the threshold of 1, with the exception of THQ for Al in May (0.92). This element also strongly affects the hazard index.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Seasonal variations of macro-, micro-, and toxic elements in tissues of vimba bream (Vimba vimba) from the Danube River near Belgrade, Serbia",
pages = "63101-63087",
number = "44",
volume = "28",
doi = "10.1007/s11356-021-15073-0"
}

Subotić, S., Višnjić Jeftić, Ž., Bojović, S., Đikanović, V., Krpo-Cetković, J.,& Lenhardt, M.. (2021). Seasonal variations of macro-, micro-, and toxic elements in tissues of vimba bream (Vimba vimba) from the Danube River near Belgrade, Serbia. in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 28(44), 63087-63101.
https://doi.org/10.1007/s11356-021-15073-0

Subotić S, Višnjić Jeftić Ž, Bojović S, Đikanović V, Krpo-Cetković J, Lenhardt M. Seasonal variations of macro-, micro-, and toxic elements in tissues of vimba bream (Vimba vimba) from the Danube River near Belgrade, Serbia. in Environmental Science and Pollution Research. 2021;28(44):63087-63101.
doi:10.1007/s11356-021-15073-0 .

Subotić, Srđan, Višnjić Jeftić, Željka, Bojović, Srdan, Đikanović, Vesna, Krpo-Cetković, Jasmina, Lenhardt, Mirjana, "Seasonal variations of macro-, micro-, and toxic elements in tissues of vimba bream (Vimba vimba) from the Danube River near Belgrade, Serbia" in Environmental Science and Pollution Research, 28, no. 44 (2021):63087-63101,
https://doi.org/10.1007/s11356-021-15073-0 . .

TY  - JOUR
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Lenhardt, Mirjana
AU  - Krpo-Cetković, Jasmina
PY  - 2021
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1434
AB  - Vimba bream individuals (n = 157), obtained from commercial catches in 2016 and 2017 near Belgrade. were analysed to obtain information on growth and condition of this species. The total body length of sampled fish ranged from 248 to 440 mm, while the body weight ranged from 139 to 889 g. Five age classes (from 4+ to 8+) were present in the sample, with predominance of individuals aged 6+. Back-calculated annual length growth was the highest in the first year of life. The total body length defined according to the parameters of the von Bertalanffy growth curve could be calculated at any given time t using 75.07 x (1-e(-0.06x(t+1.73))), and the length-weight relationship - using LogW = 0.010 + 3.02 x logTL. The asymptotic standard body length was SL infinity = 63.86 cm and the phi-prime growth performance index was phi'= 2.53. The highest mean values of Fulton's condition factor were observed in spring of 2016, followed by spring of 2017, and in individuals aged 7+. The seasonal and age-related differences in condition were statistically significant. The lack of data, the degree of endangerment in some European countries, the status of important species for the river continuity restoration and commercial exploitation of this species, emphasise the need for future research.
PB  - Institute of Biodiversity and Ecosystem Research - Bulgarian Academy of Sciences
T2  - Acta Zoologica Bulgarica
T1  - Growth and Condition of Vimba Bream Vimba vimba ( L., 1758) (Actinopterygii: Cyprinidae) from Commercial Fisheries in the Danube River near Belgrade, Serbia
EP  - 267
IS  - 2
SP  - 261
VL  - 73
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1434
ER  - 

@article{
author = "Subotić, Srđan and Višnjić Jeftić, Željka and Lenhardt, Mirjana and Krpo-Cetković, Jasmina",
year = "2021",
abstract = "Vimba bream individuals (n = 157), obtained from commercial catches in 2016 and 2017 near Belgrade. were analysed to obtain information on growth and condition of this species. The total body length of sampled fish ranged from 248 to 440 mm, while the body weight ranged from 139 to 889 g. Five age classes (from 4+ to 8+) were present in the sample, with predominance of individuals aged 6+. Back-calculated annual length growth was the highest in the first year of life. The total body length defined according to the parameters of the von Bertalanffy growth curve could be calculated at any given time t using 75.07 x (1-e(-0.06x(t+1.73))), and the length-weight relationship - using LogW = 0.010 + 3.02 x logTL. The asymptotic standard body length was SL infinity = 63.86 cm and the phi-prime growth performance index was phi'= 2.53. The highest mean values of Fulton's condition factor were observed in spring of 2016, followed by spring of 2017, and in individuals aged 7+. The seasonal and age-related differences in condition were statistically significant. The lack of data, the degree of endangerment in some European countries, the status of important species for the river continuity restoration and commercial exploitation of this species, emphasise the need for future research.",
publisher = "Institute of Biodiversity and Ecosystem Research - Bulgarian Academy of Sciences",
journal = "Acta Zoologica Bulgarica",
title = "Growth and Condition of Vimba Bream Vimba vimba ( L., 1758) (Actinopterygii: Cyprinidae) from Commercial Fisheries in the Danube River near Belgrade, Serbia",
pages = "267-261",
number = "2",
volume = "73",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1434"
}

Subotić, S., Višnjić Jeftić, Ž., Lenhardt, M.,& Krpo-Cetković, J.. (2021). Growth and Condition of Vimba Bream Vimba vimba ( L., 1758) (Actinopterygii: Cyprinidae) from Commercial Fisheries in the Danube River near Belgrade, Serbia. in Acta Zoologica Bulgarica
Institute of Biodiversity and Ecosystem Research - Bulgarian Academy of Sciences., 73(2), 261-267.
https://hdl.handle.net/21.15107/rcub_rimsi_1434

Subotić S, Višnjić Jeftić Ž, Lenhardt M, Krpo-Cetković J. Growth and Condition of Vimba Bream Vimba vimba ( L., 1758) (Actinopterygii: Cyprinidae) from Commercial Fisheries in the Danube River near Belgrade, Serbia. in Acta Zoologica Bulgarica. 2021;73(2):261-267.
https://hdl.handle.net/21.15107/rcub_rimsi_1434 .

Subotić, Srđan, Višnjić Jeftić, Željka, Lenhardt, Mirjana, Krpo-Cetković, Jasmina, "Growth and Condition of Vimba Bream Vimba vimba ( L., 1758) (Actinopterygii: Cyprinidae) from Commercial Fisheries in the Danube River near Belgrade, Serbia" in Acta Zoologica Bulgarica, 73, no. 2 (2021):261-267,
https://hdl.handle.net/21.15107/rcub_rimsi_1434 .

TY  - CHAP
AU  - Lenhardt, Mirjana
AU  - Smederevac-Lalić, Marija
AU  - Hegediš, Aleksandar
AU  - Skorić, Stefan
AU  - Cvijanović, Gorčin
AU  - Višnjić Jeftić, Željka
AU  - Đikanović, Vesna
AU  - Jovičić, K.
AU  - Jaćimović, Milica
AU  - Jarić, Ivan
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1291
AB  - The Danube River basin represents a hotspot for the European freshwater diversity. The Danube in Serbia is characterized by a significant level of fish diversity, as well as by economically important fish resources. However, the Danube fish fauna is facing a number of adverse anthropogenic impacts, such as unsustainable fishery, habitat loss and fragmentation, water pollution and non-native species invasions. In this chapter, the major adverse impacts on the Danube fish communities in Serbia are presented, and the critical management measures are discussed, such as those related to supportive stocking and other conservation efforts. In order to ensure adequate protection and sustainable management of fish resources in the Danube in Serbia, it will be critical to introduce effective monitoring system, to establish and enforce adequate management measures, and to intensify research efforts on critical issues related to fish management and conservation.
PB  - Springer
T2  - Geobotany Studies
T1  - Human Impacts on Fish Fauna in the Danube River in Serbia: Current Status and Ecological Implications
EP  - 279
SP  - 257
DO  - 10.1007/978-3-030-37242-2_13
ER  - 

@inbook{
author = "Lenhardt, Mirjana and Smederevac-Lalić, Marija and Hegediš, Aleksandar and Skorić, Stefan and Cvijanović, Gorčin and Višnjić Jeftić, Željka and Đikanović, Vesna and Jovičić, K. and Jaćimović, Milica and Jarić, Ivan",
year = "2020",
abstract = "The Danube River basin represents a hotspot for the European freshwater diversity. The Danube in Serbia is characterized by a significant level of fish diversity, as well as by economically important fish resources. However, the Danube fish fauna is facing a number of adverse anthropogenic impacts, such as unsustainable fishery, habitat loss and fragmentation, water pollution and non-native species invasions. In this chapter, the major adverse impacts on the Danube fish communities in Serbia are presented, and the critical management measures are discussed, such as those related to supportive stocking and other conservation efforts. In order to ensure adequate protection and sustainable management of fish resources in the Danube in Serbia, it will be critical to introduce effective monitoring system, to establish and enforce adequate management measures, and to intensify research efforts on critical issues related to fish management and conservation.",
publisher = "Springer",
journal = "Geobotany Studies",
booktitle = "Human Impacts on Fish Fauna in the Danube River in Serbia: Current Status and Ecological Implications",
pages = "279-257",
doi = "10.1007/978-3-030-37242-2_13"
}

Lenhardt, M., Smederevac-Lalić, M., Hegediš, A., Skorić, S., Cvijanović, G., Višnjić Jeftić, Ž., Đikanović, V., Jovičić, K., Jaćimović, M.,& Jarić, I.. (2020). Human Impacts on Fish Fauna in the Danube River in Serbia: Current Status and Ecological Implications. in Geobotany Studies
Springer., 257-279.
https://doi.org/10.1007/978-3-030-37242-2_13

Lenhardt M, Smederevac-Lalić M, Hegediš A, Skorić S, Cvijanović G, Višnjić Jeftić Ž, Đikanović V, Jovičić K, Jaćimović M, Jarić I. Human Impacts on Fish Fauna in the Danube River in Serbia: Current Status and Ecological Implications. in Geobotany Studies. 2020;:257-279.
doi:10.1007/978-3-030-37242-2_13 .

Lenhardt, Mirjana, Smederevac-Lalić, Marija, Hegediš, Aleksandar, Skorić, Stefan, Cvijanović, Gorčin, Višnjić Jeftić, Željka, Đikanović, Vesna, Jovičić, K., Jaćimović, Milica, Jarić, Ivan, "Human Impacts on Fish Fauna in the Danube River in Serbia: Current Status and Ecological Implications" in Geobotany Studies (2020):257-279,
https://doi.org/10.1007/978-3-030-37242-2_13 . .

TY  - JOUR
AU  - Spasić, Slađana
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Lenhardt, Mirjana
PY  - 2020
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1384
AB  - Fish erythrocytes released from erythropoietic sites are immature. During circulation, they increase their own surface, while the cell body and nuclei begin to become more elliptical and less spherical during maturation. The relative abundance of different developmental stages represents an erythron profile which could be a more sensitive indicator of contamination than classic hematological indices. In this study, we analyzed blood smears of the common nase (Chondrostoma nasus) and vimba bream (Vimba vimba) with the main goal to identify the determinants of the developmental stage of erythrocytes. Based on parameters developmental stages, erythrocytes are categorized into immature, intermediate or mature. In this investigation we have used four classification methods: the Two Step Cluster analysis, the K-Means Cluster analysis, and Neural Networks-Multilayer Perceptron and an Ordinal Regression model. Our findings clearly justify that Multilayers Perceptron and OLR models are appropriate to classify the developmental stage of fish erythrocytes in both species.
PB  - Springernature, London
T2  - Pattern Recognition and Image Analysis
T1  - Application of Different Classification Methods to Determine the Developmental Stage of Fish Erythrocytes of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimba vimba)
EP  - 51
IS  - 1
SP  - 43
VL  - 30
DO  - 10.1134/S1054661820010150
ER  - 

@article{
author = "Spasić, Slađana and Subotić, Srđan and Višnjić Jeftić, Željka and Lenhardt, Mirjana",
year = "2020",
abstract = "Fish erythrocytes released from erythropoietic sites are immature. During circulation, they increase their own surface, while the cell body and nuclei begin to become more elliptical and less spherical during maturation. The relative abundance of different developmental stages represents an erythron profile which could be a more sensitive indicator of contamination than classic hematological indices. In this study, we analyzed blood smears of the common nase (Chondrostoma nasus) and vimba bream (Vimba vimba) with the main goal to identify the determinants of the developmental stage of erythrocytes. Based on parameters developmental stages, erythrocytes are categorized into immature, intermediate or mature. In this investigation we have used four classification methods: the Two Step Cluster analysis, the K-Means Cluster analysis, and Neural Networks-Multilayer Perceptron and an Ordinal Regression model. Our findings clearly justify that Multilayers Perceptron and OLR models are appropriate to classify the developmental stage of fish erythrocytes in both species.",
publisher = "Springernature, London",
journal = "Pattern Recognition and Image Analysis",
title = "Application of Different Classification Methods to Determine the Developmental Stage of Fish Erythrocytes of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimba vimba)",
pages = "51-43",
number = "1",
volume = "30",
doi = "10.1134/S1054661820010150"
}

Spasić, S., Subotić, S., Višnjić Jeftić, Ž.,& Lenhardt, M.. (2020). Application of Different Classification Methods to Determine the Developmental Stage of Fish Erythrocytes of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimba vimba). in Pattern Recognition and Image Analysis
Springernature, London., 30(1), 43-51.
https://doi.org/10.1134/S1054661820010150

Spasić S, Subotić S, Višnjić Jeftić Ž, Lenhardt M. Application of Different Classification Methods to Determine the Developmental Stage of Fish Erythrocytes of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimba vimba). in Pattern Recognition and Image Analysis. 2020;30(1):43-51.
doi:10.1134/S1054661820010150 .

Spasić, Slađana, Subotić, Srđan, Višnjić Jeftić, Željka, Lenhardt, Mirjana, "Application of Different Classification Methods to Determine the Developmental Stage of Fish Erythrocytes of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimba vimba)" in Pattern Recognition and Image Analysis, 30, no. 1 (2020):43-51,
https://doi.org/10.1134/S1054661820010150 . .

TY  - CONF
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarevic, Margareta
AU  - Kostić-Vuković, Jovana
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1736
AB  - Fish communities are excellent indicators of biological and ecological integrity due to 
their continuous exposure to water conditions. Freshwater biodiversity constitute a valu able natural resource but extensive pollution of freshwater can result in biodiversity de cline. For that reason it is important to employ bioassays for purpose of detecting these 
conditions. In this work we monitored chub and bream species in 2 reservoirs, Uvac and 
Garasi, and 4 rivers: Dunav, Sava, Pestan and Beljanica. For assessment of metal and 
metalloid in fish tissues (liver, gills, gonads and muscle) we have used ICP-OES. The 
comet assay or single cell gel electrophoresis (SCGE) was selected as an in vivo geno toxicity assay, for measuring DNA damage in blood, liver and gills. Histopathological 
alterations were monitored in liver and gills.The high quality of Uvac water was con firmed by low values of DNA damage in all tissues compared to other sites. An analysis 
metals in tissues showed a high degree of their differentiation, as well as significant dif ferences in the distribution of tissue elements between the sites tested. The highest con centrations of most of the analyzed metals were found in gills, liver and gonads, and the 
lowest in muscles at all sites. In chub, blood showed the lowest DNA damage compared 
to liver and gills, while in all breams DNA damage was the highest in blood cells, follow ing gills and liver. Histopathological analysis performed on bream specimens on the Sava 
and Danube River revealed a higher degree of alterations in liver compared to the gills.
PB  - Institute of Marine Biology, University of Montenegro
C3  - International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.
T1  - Significance of genotoxicity and toxicity evaluation of freshwater bodies
SP  - 114
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1736
ER  - 

@conference{
author = "Sunjog, Karolina and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Kostić-Vuković, Jovana and Višnjić Jeftić, Željka and Gačić, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2019",
abstract = "Fish communities are excellent indicators of biological and ecological integrity due to 
their continuous exposure to water conditions. Freshwater biodiversity constitute a valu able natural resource but extensive pollution of freshwater can result in biodiversity de cline. For that reason it is important to employ bioassays for purpose of detecting these 
conditions. In this work we monitored chub and bream species in 2 reservoirs, Uvac and 
Garasi, and 4 rivers: Dunav, Sava, Pestan and Beljanica. For assessment of metal and 
metalloid in fish tissues (liver, gills, gonads and muscle) we have used ICP-OES. The 
comet assay or single cell gel electrophoresis (SCGE) was selected as an in vivo geno toxicity assay, for measuring DNA damage in blood, liver and gills. Histopathological 
alterations were monitored in liver and gills.The high quality of Uvac water was con firmed by low values of DNA damage in all tissues compared to other sites. An analysis 
metals in tissues showed a high degree of their differentiation, as well as significant dif ferences in the distribution of tissue elements between the sites tested. The highest con centrations of most of the analyzed metals were found in gills, liver and gonads, and the 
lowest in muscles at all sites. In chub, blood showed the lowest DNA damage compared 
to liver and gills, while in all breams DNA damage was the highest in blood cells, follow ing gills and liver. Histopathological analysis performed on bream specimens on the Sava 
and Danube River revealed a higher degree of alterations in liver compared to the gills.",
publisher = "Institute of Marine Biology, University of Montenegro",
journal = "International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.",
title = "Significance of genotoxicity and toxicity evaluation of freshwater bodies",
pages = "114",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1736"
}

Sunjog, K., Kolarević, S., Kracun-Kolarevic, M., Kostić-Vuković, J., Višnjić Jeftić, Ž., Gačić, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2019). Significance of genotoxicity and toxicity evaluation of freshwater bodies. in International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.
Institute of Marine Biology, University of Montenegro., 114.
https://hdl.handle.net/21.15107/rcub_rimsi_1736

Sunjog K, Kolarević S, Kracun-Kolarevic M, Kostić-Vuković J, Višnjić Jeftić Ž, Gačić Z, Lenhardt M, Vuković-Gačić B. Significance of genotoxicity and toxicity evaluation of freshwater bodies. in International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro.. 2019;:114.
https://hdl.handle.net/21.15107/rcub_rimsi_1736 .

Sunjog, Karolina, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Kostić-Vuković, Jovana, Višnjić Jeftić, Željka, Gačić, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Significance of genotoxicity and toxicity evaluation of freshwater bodies" in International Conference Adriatic Biodiversity Protection – AdriBioPro2019, Kotor, Montenegro. (2019):114,
https://hdl.handle.net/21.15107/rcub_rimsi_1736 .

TY  - CONF
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Lenhardt, Mirjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1785
AB  - Pontic shad (Alosa immaculata) is a migratory fish species, which undergoes long migrations along the Danube River, from Black Sea to the first obstacle that stops the migration the Iron Gate II dam.
Individuals used in this study were caught using drift nets, in 2018 (n=11) and 2019 (n=11), few kilometres down stream of the Iron Gate II Dam, near village of Prahovo, Serbia. Blood samples were taken from live individuals and two blood smears were prepared, on microscopic slides, for each individual. Slides were stained with Bio-Diff kit (Bio Optica, Milano, Italy) and observed and photographed under DM RB photomicroscope (Leica, Wetzlar, Germany). Pictures were analysed using the Digimizer image analysis software which automatically calculated celular and nuclear parameters of red blood cells.
The proportion of immature, intermediate, and mature erythrocytes were calculated and the differences in cellular and nuclear parameters were tested using the Mann-Whitney U test. Average length of the fish caught at 2018 was 31.9±3.9 cm, with the average weight of 188.9±48.9 g. Individuals from 2019 sampling had an average length of 29.6±2.7 cm, and the average weight of 147.2±57.5 g. Average value of Fulton’s condition factor did not differ between sampling seasons (K=0.57). Overall, in both years, immature erythrocytes constitute the majority (>57%) of analysed red blood cells. Individuals caught at 2018 had higher proportion of immature and intermediate cells, while fish caught at 2019 had higher proportion of mature cells- Man-Whitney U test showed no statistically significant differences in cell area, parameter and length between fish caught at different years.
The same morphometirc parameters, observed for nuceus, showed statistically significant differences. Our study shows that anadromous fish, such as pontic shad, which undergoes long migrations, have 2-2.5 times higher proportion of immature erytrocytes then potamodromous vimba bream (Vimba vimba), a medium distance migratory fish species.
PB  - GEOMORPHOLOGIA SLOVACA ET BOHEMICA
C3  - 7th Aquatic biodiversity international conference, Sibiu, Romania
T1  - Erythrocyte morphometry in pontic shad (Alosa immaculata).
SP  - 20
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1785
ER  - 

@conference{
author = "Subotić, Srđan and Višnjić Jeftić, Željka and Lenhardt, Mirjana",
year = "2019",
abstract = "Pontic shad (Alosa immaculata) is a migratory fish species, which undergoes long migrations along the Danube River, from Black Sea to the first obstacle that stops the migration the Iron Gate II dam.
Individuals used in this study were caught using drift nets, in 2018 (n=11) and 2019 (n=11), few kilometres down stream of the Iron Gate II Dam, near village of Prahovo, Serbia. Blood samples were taken from live individuals and two blood smears were prepared, on microscopic slides, for each individual. Slides were stained with Bio-Diff kit (Bio Optica, Milano, Italy) and observed and photographed under DM RB photomicroscope (Leica, Wetzlar, Germany). Pictures were analysed using the Digimizer image analysis software which automatically calculated celular and nuclear parameters of red blood cells.
The proportion of immature, intermediate, and mature erythrocytes were calculated and the differences in cellular and nuclear parameters were tested using the Mann-Whitney U test. Average length of the fish caught at 2018 was 31.9±3.9 cm, with the average weight of 188.9±48.9 g. Individuals from 2019 sampling had an average length of 29.6±2.7 cm, and the average weight of 147.2±57.5 g. Average value of Fulton’s condition factor did not differ between sampling seasons (K=0.57). Overall, in both years, immature erythrocytes constitute the majority (>57%) of analysed red blood cells. Individuals caught at 2018 had higher proportion of immature and intermediate cells, while fish caught at 2019 had higher proportion of mature cells- Man-Whitney U test showed no statistically significant differences in cell area, parameter and length between fish caught at different years.
The same morphometirc parameters, observed for nuceus, showed statistically significant differences. Our study shows that anadromous fish, such as pontic shad, which undergoes long migrations, have 2-2.5 times higher proportion of immature erytrocytes then potamodromous vimba bream (Vimba vimba), a medium distance migratory fish species.",
publisher = "GEOMORPHOLOGIA SLOVACA ET BOHEMICA",
journal = "7th Aquatic biodiversity international conference, Sibiu, Romania",
title = "Erythrocyte morphometry in pontic shad (Alosa immaculata).",
pages = "20",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1785"
}

Subotić, S., Višnjić Jeftić, Ž.,& Lenhardt, M.. (2019). Erythrocyte morphometry in pontic shad (Alosa immaculata).. in 7th Aquatic biodiversity international conference, Sibiu, Romania
GEOMORPHOLOGIA SLOVACA ET BOHEMICA., 20.
https://hdl.handle.net/21.15107/rcub_rimsi_1785

Subotić S, Višnjić Jeftić Ž, Lenhardt M. Erythrocyte morphometry in pontic shad (Alosa immaculata).. in 7th Aquatic biodiversity international conference, Sibiu, Romania. 2019;:20.
https://hdl.handle.net/21.15107/rcub_rimsi_1785 .

Subotić, Srđan, Višnjić Jeftić, Željka, Lenhardt, Mirjana, "Erythrocyte morphometry in pontic shad (Alosa immaculata)." in 7th Aquatic biodiversity international conference, Sibiu, Romania (2019):20,
https://hdl.handle.net/21.15107/rcub_rimsi_1785 .

TY  - CONF
AU  - Jovičić, Katarina
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Krpo-Ćetković, Jasmina
AU  - Hegedis, Aleksandar
AU  - Lenhardt, Mirjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1786
AB  - Concentrations of As, Cr, Fe, Hg, Mn, Se and Zn were assessed by ICP-OES in muscle, gills, liver and gonads of ruffe and European perch of different age classes. Fish were caughtwith portablefish nets at the confluence of the Danube river and the Sava River, in Belgrade. Age-related differences in element concentration were assessed by ANOVA  and Tukeys (HSD) tests. In ruffe, liver is the main tissue for accumulation of Cu, Fe and Se in all age classes. Gills are the main tissue for accumalation of Mn, while gonads had a higher concentrations of all elements in liver (exept for Cr), while individuals aged 4+ had the highest element concentration in gonads (again, with the exception of Cr). However, these age-related differences in ruffe were not statistically significant. In European perch, the highest concentrations of As, Cu, Fe, Se and Zn in majority of age classes were found in the liver. Individuals aged 1+ and 2+ had the highest concentration of Hg in gills. In subsequent age classes, muscle was the main tissue for accumulation of this element. Contrary to ruffe, age-related differences were observed for Se in liver, where age classes 4+ and 5+ had higher concentrations than age-class 1+, as well as for Zn in gonads, where age classes 4+ and 5+ had higher concentrations than age-class 1+, as well as for zn in gonads, where age class 1+ had higher concentration than age class 3+.
PB  - 7th Aquatic biodiversity international conference, Sibiu, Romania
C3  - 7th Aquatic biodiversity international conference, Sibiu, Romania
T1  - Age-related differences in element concentration in tissues of ruffe (Gymnocephalus cernua) and European perch (Perca fluviatilis), caught in the Danube River near Belgrade
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1786
ER  - 

@conference{
author = "Jovičić, Katarina and Subotić, Srđan and Višnjić Jeftić, Željka and Krpo-Ćetković, Jasmina and Hegedis, Aleksandar and Lenhardt, Mirjana",
year = "2019",
abstract = "Concentrations of As, Cr, Fe, Hg, Mn, Se and Zn were assessed by ICP-OES in muscle, gills, liver and gonads of ruffe and European perch of different age classes. Fish were caughtwith portablefish nets at the confluence of the Danube river and the Sava River, in Belgrade. Age-related differences in element concentration were assessed by ANOVA  and Tukeys (HSD) tests. In ruffe, liver is the main tissue for accumulation of Cu, Fe and Se in all age classes. Gills are the main tissue for accumalation of Mn, while gonads had a higher concentrations of all elements in liver (exept for Cr), while individuals aged 4+ had the highest element concentration in gonads (again, with the exception of Cr). However, these age-related differences in ruffe were not statistically significant. In European perch, the highest concentrations of As, Cu, Fe, Se and Zn in majority of age classes were found in the liver. Individuals aged 1+ and 2+ had the highest concentration of Hg in gills. In subsequent age classes, muscle was the main tissue for accumulation of this element. Contrary to ruffe, age-related differences were observed for Se in liver, where age classes 4+ and 5+ had higher concentrations than age-class 1+, as well as for Zn in gonads, where age classes 4+ and 5+ had higher concentrations than age-class 1+, as well as for zn in gonads, where age class 1+ had higher concentration than age class 3+.",
publisher = "7th Aquatic biodiversity international conference, Sibiu, Romania",
journal = "7th Aquatic biodiversity international conference, Sibiu, Romania",
title = "Age-related differences in element concentration in tissues of ruffe (Gymnocephalus cernua) and European perch (Perca fluviatilis), caught in the Danube River near Belgrade",
pages = "26",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1786"
}

Jovičić, K., Subotić, S., Višnjić Jeftić, Ž., Krpo-Ćetković, J., Hegedis, A.,& Lenhardt, M.. (2019). Age-related differences in element concentration in tissues of ruffe (Gymnocephalus cernua) and European perch (Perca fluviatilis), caught in the Danube River near Belgrade. in 7th Aquatic biodiversity international conference, Sibiu, Romania
7th Aquatic biodiversity international conference, Sibiu, Romania., 26.
https://hdl.handle.net/21.15107/rcub_rimsi_1786

Jovičić K, Subotić S, Višnjić Jeftić Ž, Krpo-Ćetković J, Hegedis A, Lenhardt M. Age-related differences in element concentration in tissues of ruffe (Gymnocephalus cernua) and European perch (Perca fluviatilis), caught in the Danube River near Belgrade. in 7th Aquatic biodiversity international conference, Sibiu, Romania. 2019;:26.
https://hdl.handle.net/21.15107/rcub_rimsi_1786 .

Jovičić, Katarina, Subotić, Srđan, Višnjić Jeftić, Željka, Krpo-Ćetković, Jasmina, Hegedis, Aleksandar, Lenhardt, Mirjana, "Age-related differences in element concentration in tissues of ruffe (Gymnocephalus cernua) and European perch (Perca fluviatilis), caught in the Danube River near Belgrade" in 7th Aquatic biodiversity international conference, Sibiu, Romania (2019):26,
https://hdl.handle.net/21.15107/rcub_rimsi_1786 .

TY  - CONF
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vuković-Gačić, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1723
AB  - Water pollution represents one of the main threats of global freshwater diversity. Untreated urban wastewaters are the source of both microbiological and chemical pollution. In exposed organisms, pollution affects different levels of biological organisation, from molecular to community level. Due to their role in aquatic ecosystems and vulnerability to pollution fish represent one of the key elements of ecosystem monitoring programs. Microbiological indicators of faecal pollution such as total coliforms, E. coli and enterococci are reliable indicators of the untreated urban wastewaters. They may be detected and quantified by fast and reliable enzymatic methods and most probable number (MPN) approach. Analysis of metals and metalloids concentrations in fish tissues indicate the exposure of fish to specific elements and can be used as a biomarker of accumulation. The single cell gel electrophoresis or comet assay is widely used in ecogenotoxicological studies for the assessment of the DNA damage as a biomarker of exposure to pollution. Histopathological alterations in fish tissues reveal changes at the middle level of biological organisation and are used as a biomarker of effect. Since each fish tissue responds differently to pollution it is recommended to perform these bioassays on multiple types of tissues, i.e.: blood, gills, liver, gonads, skin and muscle.
Analysis of different biomarkers response can give information about the early response of biota to pollution, before the changes in population structure and a decrease of individuals occur.
PB  - Institute of Marine Biology, University of Montenegro
C3  - International Conference Adriatic Biodiversity Protection AdriBioPro2019
T1  - Bioassays in assessment of environmental pollution
SP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1723
ER  - 

@conference{
author = "Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Višnjić Jeftić, Željka and Gačić, Zoran and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vuković-Gačić, Branka",
year = "2019",
abstract = "Water pollution represents one of the main threats of global freshwater diversity. Untreated urban wastewaters are the source of both microbiological and chemical pollution. In exposed organisms, pollution affects different levels of biological organisation, from molecular to community level. Due to their role in aquatic ecosystems and vulnerability to pollution fish represent one of the key elements of ecosystem monitoring programs. Microbiological indicators of faecal pollution such as total coliforms, E. coli and enterococci are reliable indicators of the untreated urban wastewaters. They may be detected and quantified by fast and reliable enzymatic methods and most probable number (MPN) approach. Analysis of metals and metalloids concentrations in fish tissues indicate the exposure of fish to specific elements and can be used as a biomarker of accumulation. The single cell gel electrophoresis or comet assay is widely used in ecogenotoxicological studies for the assessment of the DNA damage as a biomarker of exposure to pollution. Histopathological alterations in fish tissues reveal changes at the middle level of biological organisation and are used as a biomarker of effect. Since each fish tissue responds differently to pollution it is recommended to perform these bioassays on multiple types of tissues, i.e.: blood, gills, liver, gonads, skin and muscle.
Analysis of different biomarkers response can give information about the early response of biota to pollution, before the changes in population structure and a decrease of individuals occur.",
publisher = "Institute of Marine Biology, University of Montenegro",
journal = "International Conference Adriatic Biodiversity Protection AdriBioPro2019",
title = "Bioassays in assessment of environmental pollution",
pages = "115",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1723"
}

Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Sunjog, K., Višnjić Jeftić, Ž., Gačić, Z., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vuković-Gačić, B.. (2019). Bioassays in assessment of environmental pollution. in International Conference Adriatic Biodiversity Protection AdriBioPro2019
Institute of Marine Biology, University of Montenegro., 115.
https://hdl.handle.net/21.15107/rcub_rimsi_1723

Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Sunjog K, Višnjić Jeftić Ž, Gačić Z, Raskovic B, Poleksic V, Lenhardt M, Vuković-Gačić B. Bioassays in assessment of environmental pollution. in International Conference Adriatic Biodiversity Protection AdriBioPro2019. 2019;:115.
https://hdl.handle.net/21.15107/rcub_rimsi_1723 .

Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Višnjić Jeftić, Željka, Gačić, Zoran, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Bioassays in assessment of environmental pollution" in International Conference Adriatic Biodiversity Protection AdriBioPro2019 (2019):115,
https://hdl.handle.net/21.15107/rcub_rimsi_1723 .

TY  - CONF
AU  - Spasić, Slađana
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Lenhardt, Mirjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1663
AB  - Fish erythrocytes released from erythropoietic sites are immature. During circulation they increase their own surface while the cell body and nuclei begin to become more elliptical and less spherical during maturaration. The relative abundance of different developmental stages represents an erythron profile which could be a more sensitive indicator of contamination than classic hematological indices. In this study, we analyzed blood smears of the Common Nase (Chodrostoma nasus) with the main goal to identify the determinants of the developmental stage of erythrocytes. Based on parameters developmental stages, erythrocytes are categorized into immature, intermediate or mature. In this investigation we have used four classification methods: the Two Step Cluster analysis, the K-Means Cluster analysis and Neural Neworks - Multilayer Perceptron and an Ordinal Regression Model. Our findings clearly justify that Multilayers Perceptron and ORL models are appropriate to classify the developmental stage of fish erythrocytes.
PB  - Belarusian State University of lnformatics and Radioelectronics
C3  - 14th International Conference on Pattern Recognition and Information Processing – PRIP2019, Minsk, Belarus
T1  - Determination of the development stage of erythrocytes in the common nase (Chondrostoma nasus) using different classification methods.
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1663
ER  - 

@conference{
author = "Spasić, Slađana and Subotić, Srđan and Višnjić Jeftić, Željka and Lenhardt, Mirjana",
year = "2019",
abstract = "Fish erythrocytes released from erythropoietic sites are immature. During circulation they increase their own surface while the cell body and nuclei begin to become more elliptical and less spherical during maturaration. The relative abundance of different developmental stages represents an erythron profile which could be a more sensitive indicator of contamination than classic hematological indices. In this study, we analyzed blood smears of the Common Nase (Chodrostoma nasus) with the main goal to identify the determinants of the developmental stage of erythrocytes. Based on parameters developmental stages, erythrocytes are categorized into immature, intermediate or mature. In this investigation we have used four classification methods: the Two Step Cluster analysis, the K-Means Cluster analysis and Neural Neworks - Multilayer Perceptron and an Ordinal Regression Model. Our findings clearly justify that Multilayers Perceptron and ORL models are appropriate to classify the developmental stage of fish erythrocytes.",
publisher = "Belarusian State University of lnformatics and Radioelectronics",
journal = "14th International Conference on Pattern Recognition and Information Processing – PRIP2019, Minsk, Belarus",
title = "Determination of the development stage of erythrocytes in the common nase (Chondrostoma nasus) using different classification methods.",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1663"
}

Spasić, S., Subotić, S., Višnjić Jeftić, Ž.,& Lenhardt, M.. (2019). Determination of the development stage of erythrocytes in the common nase (Chondrostoma nasus) using different classification methods.. in 14th International Conference on Pattern Recognition and Information Processing – PRIP2019, Minsk, Belarus
Belarusian State University of lnformatics and Radioelectronics..
https://hdl.handle.net/21.15107/rcub_rimsi_1663

Spasić S, Subotić S, Višnjić Jeftić Ž, Lenhardt M. Determination of the development stage of erythrocytes in the common nase (Chondrostoma nasus) using different classification methods.. in 14th International Conference on Pattern Recognition and Information Processing – PRIP2019, Minsk, Belarus. 2019;.
https://hdl.handle.net/21.15107/rcub_rimsi_1663 .

Spasić, Slađana, Subotić, Srđan, Višnjić Jeftić, Željka, Lenhardt, Mirjana, "Determination of the development stage of erythrocytes in the common nase (Chondrostoma nasus) using different classification methods." in 14th International Conference on Pattern Recognition and Information Processing – PRIP2019, Minsk, Belarus (2019),
https://hdl.handle.net/21.15107/rcub_rimsi_1663 .

TY  - CONF
AU  - Gačić, Zoran
AU  - Kostić-Vuković, Jovana
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Višnjić Jeftić, Željka
AU  - Raskovic, Bozidar
AU  - Poleksic, Vesna
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1692
AB  - Untreated urban wastewaters impose high pressure on receiving water bodies. Pollution pressure may triger a cascade of events in exposed organisms, affecting different levels of biological organisation. Fish are a widely used group of bioindicators in ecogenotoxicological
studies, due to their position in the aquatic trophic web and role in human nutrition. In this work, chub (Squalius cephalus) and bream species (Abramis brama, Blicca bjoerkna, Ballerus sapa) were monitored at reservoirs, Uvac and Garasi, and rivers: Dunav, Sava, Pestan, and Beljanica. Analysis of metals and metalloids in liver, gills, gonads, and muscle was performed by ICP-OES and used as a biomarker of accumulation. The single cell gel electrophoresis, comet assay, was used to assess DNA damage level in blood, liver and gills, as a biomarker of exposure.
Histopathological alterations were monitored in liver and gills of breams from the Sava and Danube Rivers, as a biomarker of effect. Analysis of the accumulation of metals and metalloids in different tissues showed a high level of differentiation, as well as significant differences in tissues distribution of elements between the studied sites. The highest concentrations of metals and metalloids were present in gills, liver and gonads, and the lowestin muscle. Uvac showed the highest quality of water which was confirmed by the low values of DNA damage in all tissues compared to the other sites. In chub, blood showed the lowest level of DNA damage compared to liver and gills, while in all bream species DNA damage was the highest in blood cells, following gills and liver. Histopathological analysis of breams showed a higher degree of alterations in liver compared to the gills. This study highlighted the importance of the multiple biomarkers approach in obtaining information about the response of biota to pollution by analyzing changes at different levels of biological organization.
PB  - EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY
C3  - 47th Annual Meeting of EEMGS, Renn, France
T1  - Bioassays in assessment of genotoxicity and toxicity of freshwater bodies
EP  - 73
SP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1692
ER  - 

@conference{
author = "Gačić, Zoran and Kostić-Vuković, Jovana and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Višnjić Jeftić, Željka and Raskovic, Bozidar and Poleksic, Vesna and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2019",
abstract = "Untreated urban wastewaters impose high pressure on receiving water bodies. Pollution pressure may triger a cascade of events in exposed organisms, affecting different levels of biological organisation. Fish are a widely used group of bioindicators in ecogenotoxicological
studies, due to their position in the aquatic trophic web and role in human nutrition. In this work, chub (Squalius cephalus) and bream species (Abramis brama, Blicca bjoerkna, Ballerus sapa) were monitored at reservoirs, Uvac and Garasi, and rivers: Dunav, Sava, Pestan, and Beljanica. Analysis of metals and metalloids in liver, gills, gonads, and muscle was performed by ICP-OES and used as a biomarker of accumulation. The single cell gel electrophoresis, comet assay, was used to assess DNA damage level in blood, liver and gills, as a biomarker of exposure.
Histopathological alterations were monitored in liver and gills of breams from the Sava and Danube Rivers, as a biomarker of effect. Analysis of the accumulation of metals and metalloids in different tissues showed a high level of differentiation, as well as significant differences in tissues distribution of elements between the studied sites. The highest concentrations of metals and metalloids were present in gills, liver and gonads, and the lowestin muscle. Uvac showed the highest quality of water which was confirmed by the low values of DNA damage in all tissues compared to the other sites. In chub, blood showed the lowest level of DNA damage compared to liver and gills, while in all bream species DNA damage was the highest in blood cells, following gills and liver. Histopathological analysis of breams showed a higher degree of alterations in liver compared to the gills. This study highlighted the importance of the multiple biomarkers approach in obtaining information about the response of biota to pollution by analyzing changes at different levels of biological organization.",
publisher = "EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY",
journal = "47th Annual Meeting of EEMGS, Renn, France",
title = "Bioassays in assessment of genotoxicity and toxicity of freshwater bodies",
pages = "73-72",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1692"
}

Gačić, Z., Kostić-Vuković, J., Kolarević, S., Kračun-Kolarević, M., Sunjog, K., Višnjić Jeftić, Ž., Raskovic, B., Poleksic, V., Lenhardt, M.,& Vukovic-Gacic, B.. (2019). Bioassays in assessment of genotoxicity and toxicity of freshwater bodies. in 47th Annual Meeting of EEMGS, Renn, France
EUROPEAN ENVIRONMENTAL MUTAGENESIS & GENOMICS SOCIETY., 72-73.
https://hdl.handle.net/21.15107/rcub_rimsi_1692

Gačić Z, Kostić-Vuković J, Kolarević S, Kračun-Kolarević M, Sunjog K, Višnjić Jeftić Ž, Raskovic B, Poleksic V, Lenhardt M, Vukovic-Gacic B. Bioassays in assessment of genotoxicity and toxicity of freshwater bodies. in 47th Annual Meeting of EEMGS, Renn, France. 2019;:72-73.
https://hdl.handle.net/21.15107/rcub_rimsi_1692 .

Gačić, Zoran, Kostić-Vuković, Jovana, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Višnjić Jeftić, Željka, Raskovic, Bozidar, Poleksic, Vesna, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Bioassays in assessment of genotoxicity and toxicity of freshwater bodies" in 47th Annual Meeting of EEMGS, Renn, France (2019):72-73,
https://hdl.handle.net/21.15107/rcub_rimsi_1692 .

TY  - JOUR
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kracun-Kolarević, Margareta
AU  - Višnjić Jeftić, Željka
AU  - Gačić, Zoran
AU  - Lenhardt, Mirjana
AU  - Vukovic-Gacic, Branka
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1278
AB  - Due to the increasing industrialization, metals are discharged into all spheres of the environment, in particular, in river courses, which leads to the need for constant monitoring. Metals do not degrade into harmless end products; they are very persistent and have high potential for accumulation in biota. Metals in the fish body are accumulated in different amounts in the tissue specific matter. In relation to the biotic conditions and fish metabolism, the load of food, or the stage of the reproductive cycle, the seasonal variation of metal levels can be expected. Because of that, the objective of our present study was to analyze 15 metals and metalloids in liver, gills, muscle, and gonads of European chub (Squalius cephalus) throughout the 4 seasons, autumn, winter, spring, and summer. The specimens were collected from two rivers, Pestan and Beljanica at the Kolubara basin, and their concentrations were determined with inductively coupled plasma optical emission spectrometry (ICP-OES). Specimens from both rivers have shown similarities in metal accumulation like the highest accumulation of majority of elements in gills, lowest accumulation of majority of elements in muscle (except for Hg), and higher accumulation of some elements in summer (Cu, Fe, Zn). In addition, Cu and Fe showed affinity for liver, while Ba, Cr, Sr, and Zn were specific for gills. Also, Al, B, Fe, Ni, and Pb did not show significant differences in concentrations among different seasons in all investigated tissues.
PB  - Springer Heidelberg, Heidelberg
T2  - Environmental Science and Pollution Research
T1  - Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.)
EP  - 9243
IS  - 9
SP  - 9232
VL  - 26
DO  - 10.1007/s11356-019-04274-3
ER  - 

@article{
author = "Sunjog, Karolina and Kolarević, Stoimir and Kracun-Kolarević, Margareta and Višnjić Jeftić, Željka and Gačić, Zoran and Lenhardt, Mirjana and Vukovic-Gacic, Branka",
year = "2019",
abstract = "Due to the increasing industrialization, metals are discharged into all spheres of the environment, in particular, in river courses, which leads to the need for constant monitoring. Metals do not degrade into harmless end products; they are very persistent and have high potential for accumulation in biota. Metals in the fish body are accumulated in different amounts in the tissue specific matter. In relation to the biotic conditions and fish metabolism, the load of food, or the stage of the reproductive cycle, the seasonal variation of metal levels can be expected. Because of that, the objective of our present study was to analyze 15 metals and metalloids in liver, gills, muscle, and gonads of European chub (Squalius cephalus) throughout the 4 seasons, autumn, winter, spring, and summer. The specimens were collected from two rivers, Pestan and Beljanica at the Kolubara basin, and their concentrations were determined with inductively coupled plasma optical emission spectrometry (ICP-OES). Specimens from both rivers have shown similarities in metal accumulation like the highest accumulation of majority of elements in gills, lowest accumulation of majority of elements in muscle (except for Hg), and higher accumulation of some elements in summer (Cu, Fe, Zn). In addition, Cu and Fe showed affinity for liver, while Ba, Cr, Sr, and Zn were specific for gills. Also, Al, B, Fe, Ni, and Pb did not show significant differences in concentrations among different seasons in all investigated tissues.",
publisher = "Springer Heidelberg, Heidelberg",
journal = "Environmental Science and Pollution Research",
title = "Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.)",
pages = "9243-9232",
number = "9",
volume = "26",
doi = "10.1007/s11356-019-04274-3"
}

Sunjog, K., Kolarević, S., Kracun-Kolarević, M., Višnjić Jeftić, Ž., Gačić, Z., Lenhardt, M.,& Vukovic-Gacic, B.. (2019). Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.). in Environmental Science and Pollution Research
Springer Heidelberg, Heidelberg., 26(9), 9232-9243.
https://doi.org/10.1007/s11356-019-04274-3

Sunjog K, Kolarević S, Kracun-Kolarević M, Višnjić Jeftić Ž, Gačić Z, Lenhardt M, Vukovic-Gacic B. Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.). in Environmental Science and Pollution Research. 2019;26(9):9232-9243.
doi:10.1007/s11356-019-04274-3 .

Sunjog, Karolina, Kolarević, Stoimir, Kracun-Kolarević, Margareta, Višnjić Jeftić, Željka, Gačić, Zoran, Lenhardt, Mirjana, Vukovic-Gacic, Branka, "Seasonal variation in metal concentration in various tissues of the European chub (Squalius cephalus L.)" in Environmental Science and Pollution Research, 26, no. 9 (2019):9232-9243,
https://doi.org/10.1007/s11356-019-04274-3 . .

TY  - JOUR
AU  - Subotić, Srđan
AU  - Višnjić Jeftić, Željka
AU  - Đikanović, Vesna
AU  - Spasić, Slađana
AU  - Krpo-Cetković, Jasmina
AU  - Lenhardt, Mirjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1197
AB  - Accumulation of 17 elements in muscle and liver of common nase and vimba bream, caught between February and May 2016 in the Danube River (1173 river kilometer), were assessed by ICP-OES. The principal component analysis grouped muscle and liver samples based on element concentrations (muscle grouped by higher Ba and Sr values, and liver grouped by higher Al, B, Cd, Cr, Cu, Fe, Mn, and Zn values), but no grouping between the two species was observed. Concentrations of Ba, Cu, Fe, and Zn were significantly higher in muscle, and concentrations of Ba, Cd, Cu, and Mn in liver of common nase, while vimba bream had significantly higher concentrations of Cr and Fe in liver. Common nase has a higher affinity for bioaccumulation of Cu, Fe, and Zn in muscle, while vimba bream has a higher affinity for Al, Cd, and Cr in muscle.
PB  - Springer, New York
T2  - Bulletin of Environmental Contamination and Toxicology
T1  - Metal Accumulation in Muscle and Liver of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimbavimba) from the Danube River, Serbia: Bioindicative Aspects
EP  - 266
IS  - 2
SP  - 261
VL  - 103
DO  - 10.1007/s00128-019-02657-3
ER  - 

@article{
author = "Subotić, Srđan and Višnjić Jeftić, Željka and Đikanović, Vesna and Spasić, Slađana and Krpo-Cetković, Jasmina and Lenhardt, Mirjana",
year = "2019",
abstract = "Accumulation of 17 elements in muscle and liver of common nase and vimba bream, caught between February and May 2016 in the Danube River (1173 river kilometer), were assessed by ICP-OES. The principal component analysis grouped muscle and liver samples based on element concentrations (muscle grouped by higher Ba and Sr values, and liver grouped by higher Al, B, Cd, Cr, Cu, Fe, Mn, and Zn values), but no grouping between the two species was observed. Concentrations of Ba, Cu, Fe, and Zn were significantly higher in muscle, and concentrations of Ba, Cd, Cu, and Mn in liver of common nase, while vimba bream had significantly higher concentrations of Cr and Fe in liver. Common nase has a higher affinity for bioaccumulation of Cu, Fe, and Zn in muscle, while vimba bream has a higher affinity for Al, Cd, and Cr in muscle.",
publisher = "Springer, New York",
journal = "Bulletin of Environmental Contamination and Toxicology",
title = "Metal Accumulation in Muscle and Liver of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimbavimba) from the Danube River, Serbia: Bioindicative Aspects",
pages = "266-261",
number = "2",
volume = "103",
doi = "10.1007/s00128-019-02657-3"
}

Subotić, S., Višnjić Jeftić, Ž., Đikanović, V., Spasić, S., Krpo-Cetković, J.,& Lenhardt, M.. (2019). Metal Accumulation in Muscle and Liver of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimbavimba) from the Danube River, Serbia: Bioindicative Aspects. in Bulletin of Environmental Contamination and Toxicology
Springer, New York., 103(2), 261-266.
https://doi.org/10.1007/s00128-019-02657-3

Subotić S, Višnjić Jeftić Ž, Đikanović V, Spasić S, Krpo-Cetković J, Lenhardt M. Metal Accumulation in Muscle and Liver of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimbavimba) from the Danube River, Serbia: Bioindicative Aspects. in Bulletin of Environmental Contamination and Toxicology. 2019;103(2):261-266.
doi:10.1007/s00128-019-02657-3 .

Subotić, Srđan, Višnjić Jeftić, Željka, Đikanović, Vesna, Spasić, Slađana, Krpo-Cetković, Jasmina, Lenhardt, Mirjana, "Metal Accumulation in Muscle and Liver of the Common Nase (Chondrostoma nasus) and Vimba Bream (Vimbavimba) from the Danube River, Serbia: Bioindicative Aspects" in Bulletin of Environmental Contamination and Toxicology, 103, no. 2 (2019):261-266,
https://doi.org/10.1007/s00128-019-02657-3 . .