TY  - JOUR
AU  - Foyer, CH
AU  - Pastori, GM
AU  - Kiddle, G
AU  - Antoniw, J
AU  - Bernard, S
AU  - Veljović-Jovanović, Sonja
AU  - Noctor, G
AU  - Verrier, PJ
PY  - 2002
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/76
PB  - Elsevier Science Inc, New York
T2  - Free Radical Biology and Medicine
T1  - Vitamin C contents modulate defence and plant development via transcriptional regulation.
EP  - S170
SP  - S170
VL  - 33
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_76
ER  - 

@article{
author = "Foyer, CH and Pastori, GM and Kiddle, G and Antoniw, J and Bernard, S and Veljović-Jovanović, Sonja and Noctor, G and Verrier, PJ",
year = "2002",
publisher = "Elsevier Science Inc, New York",
journal = "Free Radical Biology and Medicine",
title = "Vitamin C contents modulate defence and plant development via transcriptional regulation.",
pages = "S170-S170",
volume = "33",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_76"
}

Foyer, C., Pastori, G., Kiddle, G., Antoniw, J., Bernard, S., Veljović-Jovanović, S., Noctor, G.,& Verrier, P.. (2002). Vitamin C contents modulate defence and plant development via transcriptional regulation.. in Free Radical Biology and Medicine
Elsevier Science Inc, New York., 33, S170-S170.
https://hdl.handle.net/21.15107/rcub_rimsi_76

Foyer C, Pastori G, Kiddle G, Antoniw J, Bernard S, Veljović-Jovanović S, Noctor G, Verrier P. Vitamin C contents modulate defence and plant development via transcriptional regulation.. in Free Radical Biology and Medicine. 2002;33:S170-S170.
https://hdl.handle.net/21.15107/rcub_rimsi_76 .

Foyer, CH, Pastori, GM, Kiddle, G, Antoniw, J, Bernard, S, Veljović-Jovanović, Sonja, Noctor, G, Verrier, PJ, "Vitamin C contents modulate defence and plant development via transcriptional regulation." in Free Radical Biology and Medicine, 33 (2002):S170-S170,
https://hdl.handle.net/21.15107/rcub_rimsi_76 .

TY  - JOUR
AU  - Veljović-Jovanović, Sonja
AU  - Noctor, G
AU  - Foyer, CH
PY  - 2002
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/72
AB  - We have examined the authenticity of two methods for determination of H2O2 in leaf tissue. We show that the high concentrations of ascorbic acid present in leaf extracts interfere with both techniques. In the chromogenic peroxidase-coupled assay, H2O2 is determined by oxidation of 3-methyl-2-benzothiazoline hydrazone (MBTH) and 3-(dimethylamino) benzoic acid (DMAB). The method yields two phases of absorbance increase as these substrates are oxidized. We show (a) that only the first phase is dependent on extracted H2O2; (b) that the slow phase is due to phenolic-dependent generation of H2O2 during the assay; and (c) that ascorbate inhibits both phases. These effects could explain both the high values and the variable results found in the literature. The chemiluminescence method involves H2O2 enhancement of ferricyanide-induced chemiluminescence of luminol (3-amino-phthal-hydrazide). Chemiluminescence of luminol is strongly inhibited by added ascorbate, suggesting that failure to remove ascorbate from extracts will cause this method to underestimate H2O2. Using the fast phase of the peroxidase-coupled assay to estimate H2O2 in extracts from which ascorbate and phenolic compounds had been removed, we obtained leaf contents of H2O2 within the range of 40-120 nmol g(-1) FW.
PB  - Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux
T2  - Plant Physiology and Biochemistry
T1  - Are leaf hydrogen peroxide concentrations commonly overestimated? The potential influence of artefactual interference by tissue phenolics and ascorbate
EP  - 507
IS  - 6-8
SP  - 501
VL  - 40
DO  - 10.1016/S0981-9428(02)01417-1
ER  - 

@article{
author = "Veljović-Jovanović, Sonja and Noctor, G and Foyer, CH",
year = "2002",
abstract = "We have examined the authenticity of two methods for determination of H2O2 in leaf tissue. We show that the high concentrations of ascorbic acid present in leaf extracts interfere with both techniques. In the chromogenic peroxidase-coupled assay, H2O2 is determined by oxidation of 3-methyl-2-benzothiazoline hydrazone (MBTH) and 3-(dimethylamino) benzoic acid (DMAB). The method yields two phases of absorbance increase as these substrates are oxidized. We show (a) that only the first phase is dependent on extracted H2O2; (b) that the slow phase is due to phenolic-dependent generation of H2O2 during the assay; and (c) that ascorbate inhibits both phases. These effects could explain both the high values and the variable results found in the literature. The chemiluminescence method involves H2O2 enhancement of ferricyanide-induced chemiluminescence of luminol (3-amino-phthal-hydrazide). Chemiluminescence of luminol is strongly inhibited by added ascorbate, suggesting that failure to remove ascorbate from extracts will cause this method to underestimate H2O2. Using the fast phase of the peroxidase-coupled assay to estimate H2O2 in extracts from which ascorbate and phenolic compounds had been removed, we obtained leaf contents of H2O2 within the range of 40-120 nmol g(-1) FW.",
publisher = "Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux",
journal = "Plant Physiology and Biochemistry",
title = "Are leaf hydrogen peroxide concentrations commonly overestimated? The potential influence of artefactual interference by tissue phenolics and ascorbate",
pages = "507-501",
number = "6-8",
volume = "40",
doi = "10.1016/S0981-9428(02)01417-1"
}

Veljović-Jovanović, S., Noctor, G.,& Foyer, C.. (2002). Are leaf hydrogen peroxide concentrations commonly overestimated? The potential influence of artefactual interference by tissue phenolics and ascorbate. in Plant Physiology and Biochemistry
Elsevier France-Editions Scientifiques Medicales Elsevier, Issy-Les-Moulineaux., 40(6-8), 501-507.
https://doi.org/10.1016/S0981-9428(02)01417-1

Veljović-Jovanović S, Noctor G, Foyer C. Are leaf hydrogen peroxide concentrations commonly overestimated? The potential influence of artefactual interference by tissue phenolics and ascorbate. in Plant Physiology and Biochemistry. 2002;40(6-8):501-507.
doi:10.1016/S0981-9428(02)01417-1 .

Veljović-Jovanović, Sonja, Noctor, G, Foyer, CH, "Are leaf hydrogen peroxide concentrations commonly overestimated? The potential influence of artefactual interference by tissue phenolics and ascorbate" in Plant Physiology and Biochemistry, 40, no. 6-8 (2002):501-507,
https://doi.org/10.1016/S0981-9428(02)01417-1 . .