TY  - JOUR
AU  - Milosavić, Nenad B
AU  - Bogdanović Pristov, Jelena
AU  - Velicković, Dusan V
AU  - Dimitrijević, Aleksandra S
AU  - Kalauzi, Aleksandar
AU  - Radotić, Ksenija
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/574
AB  - BACKGROUND: A totally new approach has been applied for mathematical modeling of the enzyme activity/pH relationship, for quantification and distribution of enzyme activity in and out of carrier pores. This is a very simple and elegant method for determination of the distribution of enzyme molecules on the surface and inside the particles, simply through measurement of enzyme activity at different pH values. RESULTS: Amyloglucosidase (AG) from Aspergillus niger was covalently immobilized onto poly(GMA-co-EGDMA) by the glutaraldehyde and periodate methods. Mathematical modeling of the pH optima for two types of covalently immobilized AG resulted in higher enzyme amounts on the surface within periodate immobilizate (67%) in comparison with glutaraldehyde immobilizate (53%). These values are modified to 64.25% for periodate immobilizate and 49.95% for glutaraldehyde immobilizate when diffusion effects are taken into account. CONCLUSION: The mathematical model applied enabled observation of the difference between the two types of coupling agents and different immobilization procedures throughout quantification of the immobilized enzyme on the matrix surface and inside pores.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Chemical Technology and Biotechnology
T1  - Study of the covalently immobilized amyloglucosidase on macroporous polymer by mathematical modeling of the pH optima
EP  - 1457
IS  - 10
SP  - 1450
VL  - 87
DO  - 10.1002/jctb.3768
ER  - 

@article{
author = "Milosavić, Nenad B and Bogdanović Pristov, Jelena and Velicković, Dusan V and Dimitrijević, Aleksandra S and Kalauzi, Aleksandar and Radotić, Ksenija",
year = "2012",
abstract = "BACKGROUND: A totally new approach has been applied for mathematical modeling of the enzyme activity/pH relationship, for quantification and distribution of enzyme activity in and out of carrier pores. This is a very simple and elegant method for determination of the distribution of enzyme molecules on the surface and inside the particles, simply through measurement of enzyme activity at different pH values. RESULTS: Amyloglucosidase (AG) from Aspergillus niger was covalently immobilized onto poly(GMA-co-EGDMA) by the glutaraldehyde and periodate methods. Mathematical modeling of the pH optima for two types of covalently immobilized AG resulted in higher enzyme amounts on the surface within periodate immobilizate (67%) in comparison with glutaraldehyde immobilizate (53%). These values are modified to 64.25% for periodate immobilizate and 49.95% for glutaraldehyde immobilizate when diffusion effects are taken into account. CONCLUSION: The mathematical model applied enabled observation of the difference between the two types of coupling agents and different immobilization procedures throughout quantification of the immobilized enzyme on the matrix surface and inside pores.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Chemical Technology and Biotechnology",
title = "Study of the covalently immobilized amyloglucosidase on macroporous polymer by mathematical modeling of the pH optima",
pages = "1457-1450",
number = "10",
volume = "87",
doi = "10.1002/jctb.3768"
}

Milosavić, N. B., Bogdanović Pristov, J., Velicković, D. V., Dimitrijević, A. S., Kalauzi, A.,& Radotić, K.. (2012). Study of the covalently immobilized amyloglucosidase on macroporous polymer by mathematical modeling of the pH optima. in Journal of Chemical Technology and Biotechnology
Wiley-Blackwell, Hoboken., 87(10), 1450-1457.
https://doi.org/10.1002/jctb.3768

Milosavić NB, Bogdanović Pristov J, Velicković DV, Dimitrijević AS, Kalauzi A, Radotić K. Study of the covalently immobilized amyloglucosidase on macroporous polymer by mathematical modeling of the pH optima. in Journal of Chemical Technology and Biotechnology. 2012;87(10):1450-1457.
doi:10.1002/jctb.3768 .

Milosavić, Nenad B, Bogdanović Pristov, Jelena, Velicković, Dusan V, Dimitrijević, Aleksandra S, Kalauzi, Aleksandar, Radotić, Ksenija, "Study of the covalently immobilized amyloglucosidase on macroporous polymer by mathematical modeling of the pH optima" in Journal of Chemical Technology and Biotechnology, 87, no. 10 (2012):1450-1457,
https://doi.org/10.1002/jctb.3768 . .