TY  - JOUR
AU  - Bolic, Bojana
AU  - Mijusković, Ana
AU  - Popovic-Bijelic, Ana
AU  - Nikolić-Kokić, Aleksandra
AU  - Spasic, Snežana
AU  - Blagojević, Duško
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/846
AB  - Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study
EP  - 23
SP  - 19
VL  - 51
DO  - 10.1016/j.niox.2015.09.008
ER  - 

@article{
author = "Bolic, Bojana and Mijusković, Ana and Popovic-Bijelic, Ana and Nikolić-Kokić, Aleksandra and Spasic, Snežana and Blagojević, Duško and Spasić, Mihajlo and Spasojević, Ivan",
year = "2015",
abstract = "Interactions of hydrogen sulfide (HS-/H2S), a reducing signaling species, with superoxide dimutases (SOD) are poorly understood. We applied low-T EPR spectroscopy to examine the effects of HS-/H2S and superoxide radical anion (O-2(-)) on metallocenters of FeSOD, MnSOD, and CuZnSOD. HS-/H2S did not affect FeSOD, whereas active centers of MnSOD and CuZnSOD were open to this agent. Cu2+ was reduced to Cu1+, while manganese appears to be released from MnSOD active center. Untreated and O-2(-) treated FeSOD and MnSOD predominantly show 5 d-electron systems, i.e. Fe3+ and Mn2+. Our study provides new details on the mechanisms of (patho)physiological effects of HS-/H2S.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study",
pages = "23-19",
volume = "51",
doi = "10.1016/j.niox.2015.09.008"
}

Bolic, B., Mijusković, A., Popovic-Bijelic, A., Nikolić-Kokić, A., Spasic, S., Blagojević, D., Spasić, M.,& Spasojević, I.. (2015). Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 51, 19-23.
https://doi.org/10.1016/j.niox.2015.09.008

Bolic B, Mijusković A, Popovic-Bijelic A, Nikolić-Kokić A, Spasic S, Blagojević D, Spasić M, Spasojević I. Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study. in Nitric Oxide-Biology and Chemistry. 2015;51:19-23.
doi:10.1016/j.niox.2015.09.008 .

Bolic, Bojana, Mijusković, Ana, Popovic-Bijelic, Ana, Nikolić-Kokić, Aleksandra, Spasic, Snežana, Blagojević, Duško, Spasić, Mihajlo, Spasojević, Ivan, "Reactions of superoxide dismutases with HS-/H2S and superoxide radical anion: An in vitro EPR study" in Nitric Oxide-Biology and Chemistry, 51 (2015):19-23,
https://doi.org/10.1016/j.niox.2015.09.008 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin-Dušić, Zorana
AU  - Spasojević, Ivan
AU  - Slavić, Marija
AU  - Mijusković, Ana
AU  - Paskulin, Roman
AU  - Miljević, Cedo
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško P.
PY  - 2015
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/889
AB  - Ethnopharmacological relevance: Ibogaine is a naturally occurring alkaloid with psychotropic and metabotropic effects, derived from the bark of the root of the West African Tabernanthe iboga plant. The tribes of Kongo basin have been using iboga as a stimulant, for medicinal purposes, and in rite of passage ceremonies, for centuries. Besides, it has been found that this drug has anti-addictive effects. Aim of the study: Previous studies have demonstrated that ibogaine changed the quantity of ATP and energy related enzymes as well as the activity of antioxidant enzymes in cells thus altering redox equilibrium in a time manner. In this work, the mechanism of its action was further studied by measuring the effects of ibogaine in human erythrocytes in vitro on ATP liberation, membrane fluidity and antioxidant enzymes activity. Materials and methods: Heparinized human blood samples were incubated with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was separated by centrifugation and the levels of ATP and uric acid were measured 10 mm after the addition of ibogaine using standard kits. The activity of copper zinc superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were measured in erythrocytes after incubation period. The stability of SOD1 activity was further tested through in vitro incubation with H2O2 and scanning of its electrophoretic profiles. Membrane fluidity was determined using an electron paramagnetic resonance spin-labelling method. Results: Results showed that ibogaine treatment of erythrocytes in vitro increased ATP concentration in the blood plasma without changes in neither erythrocytes membrane fluidity nor uric acid concentration. lbogaine also increased SOD1 activity in erythrocytes at both doses applied here. Treatment with 20 mu M also elevated GR activity after in vitro incubation at 37 degrees C. Electrophoretic profiles revealed that incubation with ibogaine mitigates H2O2 mediated suppression of SOD1 activity. Conclusion: Some of the effects of ibogaine seem to be mediated through its influence on energy metabolism, redox active processes and the effects of discrete fluctuations of individual reactive oxygen species on different levels of enzyme activities. Overall, ibogaine acts as a pro-antioxidant by increasing activity of antioxidative enzymes and as an adaptagene in oxidative distress.
PB  - Elsevier Ireland Ltd, Clare
T2  - Journal of Ethnopharmacology
T1  - Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes
EP  - 70
SP  - 64
VL  - 164
DO  - 10.1016/j.jep.2015.01.037
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin-Dušić, Zorana and Spasojević, Ivan and Slavić, Marija and Mijusković, Ana and Paskulin, Roman and Miljević, Cedo and Spasić, Mihajlo and Blagojević, Duško P.",
year = "2015",
abstract = "Ethnopharmacological relevance: Ibogaine is a naturally occurring alkaloid with psychotropic and metabotropic effects, derived from the bark of the root of the West African Tabernanthe iboga plant. The tribes of Kongo basin have been using iboga as a stimulant, for medicinal purposes, and in rite of passage ceremonies, for centuries. Besides, it has been found that this drug has anti-addictive effects. Aim of the study: Previous studies have demonstrated that ibogaine changed the quantity of ATP and energy related enzymes as well as the activity of antioxidant enzymes in cells thus altering redox equilibrium in a time manner. In this work, the mechanism of its action was further studied by measuring the effects of ibogaine in human erythrocytes in vitro on ATP liberation, membrane fluidity and antioxidant enzymes activity. Materials and methods: Heparinized human blood samples were incubated with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was separated by centrifugation and the levels of ATP and uric acid were measured 10 mm after the addition of ibogaine using standard kits. The activity of copper zinc superoxide dismutase (SOD1), catalase (CAT), glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were measured in erythrocytes after incubation period. The stability of SOD1 activity was further tested through in vitro incubation with H2O2 and scanning of its electrophoretic profiles. Membrane fluidity was determined using an electron paramagnetic resonance spin-labelling method. Results: Results showed that ibogaine treatment of erythrocytes in vitro increased ATP concentration in the blood plasma without changes in neither erythrocytes membrane fluidity nor uric acid concentration. lbogaine also increased SOD1 activity in erythrocytes at both doses applied here. Treatment with 20 mu M also elevated GR activity after in vitro incubation at 37 degrees C. Electrophoretic profiles revealed that incubation with ibogaine mitigates H2O2 mediated suppression of SOD1 activity. Conclusion: Some of the effects of ibogaine seem to be mediated through its influence on energy metabolism, redox active processes and the effects of discrete fluctuations of individual reactive oxygen species on different levels of enzyme activities. Overall, ibogaine acts as a pro-antioxidant by increasing activity of antioxidative enzymes and as an adaptagene in oxidative distress.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Journal of Ethnopharmacology",
title = "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes",
pages = "70-64",
volume = "164",
doi = "10.1016/j.jep.2015.01.037"
}

Nikolić-Kokić, A., Oreščanin-Dušić, Z., Spasojević, I., Slavić, M., Mijusković, A., Paskulin, R., Miljević, C., Spasić, M.,& Blagojević, D. P.. (2015). Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes. in Journal of Ethnopharmacology
Elsevier Ireland Ltd, Clare., 164, 64-70.
https://doi.org/10.1016/j.jep.2015.01.037

Nikolić-Kokić A, Oreščanin-Dušić Z, Spasojević I, Slavić M, Mijusković A, Paskulin R, Miljević C, Spasić M, Blagojević DP. Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes. in Journal of Ethnopharmacology. 2015;164:64-70.
doi:10.1016/j.jep.2015.01.037 .

Nikolić-Kokić, Aleksandra, Oreščanin-Dušić, Zorana, Spasojević, Ivan, Slavić, Marija, Mijusković, Ana, Paskulin, Roman, Miljević, Cedo, Spasić, Mihajlo, Blagojević, Duško P., "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in human erythrocytes" in Journal of Ethnopharmacology, 164 (2015):64-70,
https://doi.org/10.1016/j.jep.2015.01.037 . .

TY  - GEN
AU  - Mijusković, Ana
AU  - Oreščanin-Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavić, Marija
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
AU  - Blagojević, Duško P
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/774
AB  - H2S was shown as an uterine relaxant. However, the signaling pathways, including ion channels regulated by H2S mediating relaxation in uterus are still unknown. The effects on contractility in response to sodium sulphide Na2S were examined on myometrial strips from virgin Wistar rats. Our results showed that Na2S induces concentration-dependent relaxations affecting amplitude as well as frequency of contractions. Activation of potassium channels, and in particular of KATP, was one of the primary mechanisms proposed, responsible for the relaxing effects of H2S. Sodium sulphide (20–200 × 10−6 M) inhibits myometrial contractility through a K-channel-independent mechanism. An inhibitor of 4,4 – inhibitor of Cl-/HCO3- exchanger and/or Cl− channel, DIDS, caused a significant rightward shift of the Na2S concentration–response curve. We performed experiments aimed at different Ca2+ concentrations, using spontaneous, calcium and KCl (15 mM and 75 mM) induced contractions, as well as pharmacological inhibitors of calcium channels and modulators, showing that Na2S induced relaxation is dependent on the precontractile agent used. Taken together, our results suggests that decreased frequency induced by Na2S could be a consequence of a alterated pacemaker cells which might be related for Ca2+ events originates from sarco endoplasmatic reticulum and/or mitochondria.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Nitric Oxide-Biology and Chemistry
T1  - Sodium sulphide relaxation of rat uterus is related to calcium signaling
EP  - S37
SP  - S36
VL  - 39
DO  - 10.1016/j.niox.2014.03.119
ER  - 

@misc{
author = "Mijusković, Ana and Oreščanin-Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavić, Marija and Spasić, Mihajlo and Spasojević, Ivan and Blagojević, Duško P",
year = "2014",
abstract = "H2S was shown as an uterine relaxant. However, the signaling pathways, including ion channels regulated by H2S mediating relaxation in uterus are still unknown. The effects on contractility in response to sodium sulphide Na2S were examined on myometrial strips from virgin Wistar rats. Our results showed that Na2S induces concentration-dependent relaxations affecting amplitude as well as frequency of contractions. Activation of potassium channels, and in particular of KATP, was one of the primary mechanisms proposed, responsible for the relaxing effects of H2S. Sodium sulphide (20–200 × 10−6 M) inhibits myometrial contractility through a K-channel-independent mechanism. An inhibitor of 4,4 – inhibitor of Cl-/HCO3- exchanger and/or Cl− channel, DIDS, caused a significant rightward shift of the Na2S concentration–response curve. We performed experiments aimed at different Ca2+ concentrations, using spontaneous, calcium and KCl (15 mM and 75 mM) induced contractions, as well as pharmacological inhibitors of calcium channels and modulators, showing that Na2S induced relaxation is dependent on the precontractile agent used. Taken together, our results suggests that decreased frequency induced by Na2S could be a consequence of a alterated pacemaker cells which might be related for Ca2+ events originates from sarco endoplasmatic reticulum and/or mitochondria.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Sodium sulphide relaxation of rat uterus is related to calcium signaling",
pages = "S37-S36",
volume = "39",
doi = "10.1016/j.niox.2014.03.119"
}

Mijusković, A., Oreščanin-Dušić, Z., Nikolić-Kokić, A., Slavić, M., Spasić, M., Spasojević, I.,& Blagojević, D. P.. (2014). Sodium sulphide relaxation of rat uterus is related to calcium signaling. in Nitric Oxide-Biology and Chemistry
Academic Press Inc Elsevier Science, San Diego., 39, S36-S37.
https://doi.org/10.1016/j.niox.2014.03.119

Mijusković A, Oreščanin-Dušić Z, Nikolić-Kokić A, Slavić M, Spasić M, Spasojević I, Blagojević DP. Sodium sulphide relaxation of rat uterus is related to calcium signaling. in Nitric Oxide-Biology and Chemistry. 2014;39:S36-S37.
doi:10.1016/j.niox.2014.03.119 .

Mijusković, Ana, Oreščanin-Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavić, Marija, Spasić, Mihajlo, Spasojević, Ivan, Blagojević, Duško P, "Sodium sulphide relaxation of rat uterus is related to calcium signaling" in Nitric Oxide-Biology and Chemistry, 39 (2014):S36-S37,
https://doi.org/10.1016/j.niox.2014.03.119 . .

TY  - JOUR
AU  - Mijusković, Ana
AU  - Oreščanin-Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavić, Marija
AU  - Spasić, Mihajlo
AU  - Spasojević, Ivan
AU  - Blagojević, Duško P
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/787
AB  - Background: Our aim was to investigate the effect of methanethiol (CH3SH) on contractility of rat uterus and activities of redox-active enzymes, and to compare them with the effect of sodium sulphide (Na2S), a hydrogen sulphide (H2S/HS-) donor. Methods: Uteri were isolated from virgin Wistar rats, divided into six groups, controls (untreated uteri allowed to contract spontaneously and in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced). Underlying antioxidative enzyme activities (superoxide dismutase - SOD, glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or Na2S-treated uteri were compared to controls. Results: Our experiments showed that CH3SH and Na2S provoked reversible relaxation of both spontaneous and Ca2+ induced uterine contractions. The dose-response curves differed in shape, and CH3SH curve was shifted to higher concentration compared to H2S/HS-. The effects of Na2S fitted sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked increased SOD activity and decreased GR activity. However, Na2S (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+ stimulated uteri, while the activity of GSHPx was increased in both types of active uteri. Conclusion: Our results imply that CH3SH may have a constructive role in the control of muscle function and metabolism. Observed differences between CH3SH and H2S/HS- could be attributed to a larger moiety that is present in CH3SH compared to H2S, but they are more likely to be a consequence of the specific actions of HS-, in relation to its negative charge.
PB  - Polish Acad Sciences Inst Pharmacology, Krakow
T2  - Pharmacological Reports
T1  - Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity
EP  - 379
IS  - 3
SP  - 373
VL  - 66
DO  - 10.1016/j.pharep.2013.12.012
ER  - 

@article{
author = "Mijusković, Ana and Oreščanin-Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavić, Marija and Spasić, Mihajlo and Spasojević, Ivan and Blagojević, Duško P",
year = "2014",
abstract = "Background: Our aim was to investigate the effect of methanethiol (CH3SH) on contractility of rat uterus and activities of redox-active enzymes, and to compare them with the effect of sodium sulphide (Na2S), a hydrogen sulphide (H2S/HS-) donor. Methods: Uteri were isolated from virgin Wistar rats, divided into six groups, controls (untreated uteri allowed to contract spontaneously and in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced). Underlying antioxidative enzyme activities (superoxide dismutase - SOD, glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or Na2S-treated uteri were compared to controls. Results: Our experiments showed that CH3SH and Na2S provoked reversible relaxation of both spontaneous and Ca2+ induced uterine contractions. The dose-response curves differed in shape, and CH3SH curve was shifted to higher concentration compared to H2S/HS-. The effects of Na2S fitted sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked increased SOD activity and decreased GR activity. However, Na2S (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+ stimulated uteri, while the activity of GSHPx was increased in both types of active uteri. Conclusion: Our results imply that CH3SH may have a constructive role in the control of muscle function and metabolism. Observed differences between CH3SH and H2S/HS- could be attributed to a larger moiety that is present in CH3SH compared to H2S, but they are more likely to be a consequence of the specific actions of HS-, in relation to its negative charge.",
publisher = "Polish Acad Sciences Inst Pharmacology, Krakow",
journal = "Pharmacological Reports",
title = "Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity",
pages = "379-373",
number = "3",
volume = "66",
doi = "10.1016/j.pharep.2013.12.012"
}

Mijusković, A., Oreščanin-Dušić, Z., Nikolić-Kokić, A., Slavić, M., Spasić, M., Spasojević, I.,& Blagojević, D. P.. (2014). Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity. in Pharmacological Reports
Polish Acad Sciences Inst Pharmacology, Krakow., 66(3), 373-379.
https://doi.org/10.1016/j.pharep.2013.12.012

Mijusković A, Oreščanin-Dušić Z, Nikolić-Kokić A, Slavić M, Spasić M, Spasojević I, Blagojević DP. Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity. in Pharmacological Reports. 2014;66(3):373-379.
doi:10.1016/j.pharep.2013.12.012 .

Mijusković, Ana, Oreščanin-Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavić, Marija, Spasić, Mihajlo, Spasojević, Ivan, Blagojević, Duško P, "Comparison of the effects of methanethiol and sodium sulphide on uterine contractile activity" in Pharmacological Reports, 66, no. 3 (2014):373-379,
https://doi.org/10.1016/j.pharep.2013.12.012 . .