TY  - JOUR
AU  - Pavun, Leposava A
AU  - Dimitrić-Marković, Jasmina
AU  - Djurdjević, Predrag T
AU  - Jelikic-Stankov, Milena D
AU  - Đikanović, Daniela
AU  - Ciric, Andrija
AU  - Malesev, Dusan L
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/512
AB  - A fluorometric method, based on the fluorescence properties of the aluminium(III)-hesperidin complex, for the determination of hesperidin in human plasma and pharmaceutical forms has been developed and validated. The complex shows a strong emission in the presence of the surfactant betain sulphonate SB 12 at 476 nm with excitation at 390 nm. The linearity range for pharmaceutical forms of hesperidin was 0.06-24.4 mu g mL(-1) with a limit of detection, LOD, of 0.016 mu g mL(-1) and a limit of quantification, LOQ, of 0.049 mu g mL(-1). Recovery values in the range 99.3-99.7 % indicate good accuracy of the method. A linear dependence of the intensity of fluorescence of the complex on the concentration of hesperidin in plasma was obtained in concentration range from 0.1-12.2 mu g mL(-1). The LOD was 0.032 mu g mL(-1) while LOQ was 0.096 mu g mL(-1). Recovery values were in the range 98.4-99.8 %. The reliability of the method was checked by an LC MS/MS method for plasma samples and an HPLC/UV method for tablets with direct determination of hesperidin after separation. Linearity range in determination of hesperidin in pharmaceutical forms was obtained in the range from 0.05 to 10.00 mu g mL(-1). The LOD was 0.01 mu g mL(-1) and the LOQ was 0.03 mu g mL(-1). The linearity range for the determination of hesperidin in plasma was 0.02-10.00 mu g mL(-1) with an LOD 0.005 mu g mL(-1) and an LOQ of 0.015 mu g mL(-1). The good agreement between the two methods indicates the usability of the proposed fluorometric method for the simple, precise and accurate determination of hesperidin in clinical and quality control laboratories.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms
EP  - 1640
IS  - 11
SP  - 1625
VL  - 77
DO  - 10.2298/JSC111005060P
ER  - 

@article{
author = "Pavun, Leposava A and Dimitrić-Marković, Jasmina and Djurdjević, Predrag T and Jelikic-Stankov, Milena D and Đikanović, Daniela and Ciric, Andrija and Malesev, Dusan L",
year = "2012",
abstract = "A fluorometric method, based on the fluorescence properties of the aluminium(III)-hesperidin complex, for the determination of hesperidin in human plasma and pharmaceutical forms has been developed and validated. The complex shows a strong emission in the presence of the surfactant betain sulphonate SB 12 at 476 nm with excitation at 390 nm. The linearity range for pharmaceutical forms of hesperidin was 0.06-24.4 mu g mL(-1) with a limit of detection, LOD, of 0.016 mu g mL(-1) and a limit of quantification, LOQ, of 0.049 mu g mL(-1). Recovery values in the range 99.3-99.7 % indicate good accuracy of the method. A linear dependence of the intensity of fluorescence of the complex on the concentration of hesperidin in plasma was obtained in concentration range from 0.1-12.2 mu g mL(-1). The LOD was 0.032 mu g mL(-1) while LOQ was 0.096 mu g mL(-1). Recovery values were in the range 98.4-99.8 %. The reliability of the method was checked by an LC MS/MS method for plasma samples and an HPLC/UV method for tablets with direct determination of hesperidin after separation. Linearity range in determination of hesperidin in pharmaceutical forms was obtained in the range from 0.05 to 10.00 mu g mL(-1). The LOD was 0.01 mu g mL(-1) and the LOQ was 0.03 mu g mL(-1). The linearity range for the determination of hesperidin in plasma was 0.02-10.00 mu g mL(-1) with an LOD 0.005 mu g mL(-1) and an LOQ of 0.015 mu g mL(-1). The good agreement between the two methods indicates the usability of the proposed fluorometric method for the simple, precise and accurate determination of hesperidin in clinical and quality control laboratories.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms",
pages = "1640-1625",
number = "11",
volume = "77",
doi = "10.2298/JSC111005060P"
}

Pavun, L. A., Dimitrić-Marković, J., Djurdjević, P. T., Jelikic-Stankov, M. D., Đikanović, D., Ciric, A.,& Malesev, D. L.. (2012). Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 77(11), 1625-1640.
https://doi.org/10.2298/JSC111005060P

Pavun LA, Dimitrić-Marković J, Djurdjević PT, Jelikic-Stankov MD, Đikanović D, Ciric A, Malesev DL. Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms. in Journal of the Serbian Chemical Society. 2012;77(11):1625-1640.
doi:10.2298/JSC111005060P .

Pavun, Leposava A, Dimitrić-Marković, Jasmina, Djurdjević, Predrag T, Jelikic-Stankov, Milena D, Đikanović, Daniela, Ciric, Andrija, Malesev, Dusan L, "Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms" in Journal of the Serbian Chemical Society, 77, no. 11 (2012):1625-1640,
https://doi.org/10.2298/JSC111005060P . .