TY  - JOUR
AU  - Pavun, Leposava
AU  - Durdević, Predrag
AU  - Jelikic-Stankov, Milena
AU  - Đikanović, Daniela
AU  - Ciric, Andrija
AU  - Uskokovic-Marković, Snežana
PY  - 2014
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/799
AB  - A simple, accurate and precise method based on the fluorescence properties of the aluminum(III)-quercetin complex, for the determination of quercetin, has been developed and validated. The complex has strong emission at pH 3.30, lambda(em) = 480 nm, with lambda(ex) = 420 nm. The linearity range of quercetin determination was 1.5-60.5 ng ml(-1) with LOD 0.09 ng ml(-1) and LOQ 0.27 ng ml(-1). Recovery values in the range of 99.9-100.2% indicate a good accuracy of the method. The established method was applied for the determination of quercetin in capsules, with a recovery value of 98.3%, standard deviation of 0.22% and coefficient of variation of 0.09%. The reliability of the method was checked by the newly developed RP-HPLC/UV method for capsules with the direct determination of quercetin after separation. The good agreement between the two methods indicates the applicability of the proposed spectrofluorimetric method for quercetin determination in pharmaceutical dosage forms, with high reproducibility, and enables the direct and simple determination without its prior extraction from samples. The proposed spectrofluorimetric method has much better sensitivity and LOD and LOQ values that are about 1000 times lower than data reported in the literature.
PB  - Soc Chemists Technologists Madeconia, Skopje
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms
EP  - 215
IS  - 2
SP  - 209
VL  - 33
DO  - 10.20450/mjcce.2014.496
ER  - 

@article{
author = "Pavun, Leposava and Durdević, Predrag and Jelikic-Stankov, Milena and Đikanović, Daniela and Ciric, Andrija and Uskokovic-Marković, Snežana",
year = "2014",
abstract = "A simple, accurate and precise method based on the fluorescence properties of the aluminum(III)-quercetin complex, for the determination of quercetin, has been developed and validated. The complex has strong emission at pH 3.30, lambda(em) = 480 nm, with lambda(ex) = 420 nm. The linearity range of quercetin determination was 1.5-60.5 ng ml(-1) with LOD 0.09 ng ml(-1) and LOQ 0.27 ng ml(-1). Recovery values in the range of 99.9-100.2% indicate a good accuracy of the method. The established method was applied for the determination of quercetin in capsules, with a recovery value of 98.3%, standard deviation of 0.22% and coefficient of variation of 0.09%. The reliability of the method was checked by the newly developed RP-HPLC/UV method for capsules with the direct determination of quercetin after separation. The good agreement between the two methods indicates the applicability of the proposed spectrofluorimetric method for quercetin determination in pharmaceutical dosage forms, with high reproducibility, and enables the direct and simple determination without its prior extraction from samples. The proposed spectrofluorimetric method has much better sensitivity and LOD and LOQ values that are about 1000 times lower than data reported in the literature.",
publisher = "Soc Chemists Technologists Madeconia, Skopje",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms",
pages = "215-209",
number = "2",
volume = "33",
doi = "10.20450/mjcce.2014.496"
}

Pavun, L., Durdević, P., Jelikic-Stankov, M., Đikanović, D., Ciric, A.,& Uskokovic-Marković, S.. (2014). Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms. in Macedonian Journal of Chemistry and Chemical Engineering
Soc Chemists Technologists Madeconia, Skopje., 33(2), 209-215.
https://doi.org/10.20450/mjcce.2014.496

Pavun L, Durdević P, Jelikic-Stankov M, Đikanović D, Ciric A, Uskokovic-Marković S. Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms. in Macedonian Journal of Chemistry and Chemical Engineering. 2014;33(2):209-215.
doi:10.20450/mjcce.2014.496 .

Pavun, Leposava, Durdević, Predrag, Jelikic-Stankov, Milena, Đikanović, Daniela, Ciric, Andrija, Uskokovic-Marković, Snežana, "Spectrofluorimetric determination of quercetin in pharmaceutical dosage forms" in Macedonian Journal of Chemistry and Chemical Engineering, 33, no. 2 (2014):209-215,
https://doi.org/10.20450/mjcce.2014.496 . .

TY  - JOUR
AU  - Pavun, Leposava A
AU  - Dimitrić-Marković, Jasmina
AU  - Djurdjević, Predrag T
AU  - Jelikic-Stankov, Milena D
AU  - Đikanović, Daniela
AU  - Ciric, Andrija
AU  - Malesev, Dusan L
PY  - 2012
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/512
AB  - A fluorometric method, based on the fluorescence properties of the aluminium(III)-hesperidin complex, for the determination of hesperidin in human plasma and pharmaceutical forms has been developed and validated. The complex shows a strong emission in the presence of the surfactant betain sulphonate SB 12 at 476 nm with excitation at 390 nm. The linearity range for pharmaceutical forms of hesperidin was 0.06-24.4 mu g mL(-1) with a limit of detection, LOD, of 0.016 mu g mL(-1) and a limit of quantification, LOQ, of 0.049 mu g mL(-1). Recovery values in the range 99.3-99.7 % indicate good accuracy of the method. A linear dependence of the intensity of fluorescence of the complex on the concentration of hesperidin in plasma was obtained in concentration range from 0.1-12.2 mu g mL(-1). The LOD was 0.032 mu g mL(-1) while LOQ was 0.096 mu g mL(-1). Recovery values were in the range 98.4-99.8 %. The reliability of the method was checked by an LC MS/MS method for plasma samples and an HPLC/UV method for tablets with direct determination of hesperidin after separation. Linearity range in determination of hesperidin in pharmaceutical forms was obtained in the range from 0.05 to 10.00 mu g mL(-1). The LOD was 0.01 mu g mL(-1) and the LOQ was 0.03 mu g mL(-1). The linearity range for the determination of hesperidin in plasma was 0.02-10.00 mu g mL(-1) with an LOD 0.005 mu g mL(-1) and an LOQ of 0.015 mu g mL(-1). The good agreement between the two methods indicates the usability of the proposed fluorometric method for the simple, precise and accurate determination of hesperidin in clinical and quality control laboratories.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms
EP  - 1640
IS  - 11
SP  - 1625
VL  - 77
DO  - 10.2298/JSC111005060P
ER  - 

@article{
author = "Pavun, Leposava A and Dimitrić-Marković, Jasmina and Djurdjević, Predrag T and Jelikic-Stankov, Milena D and Đikanović, Daniela and Ciric, Andrija and Malesev, Dusan L",
year = "2012",
abstract = "A fluorometric method, based on the fluorescence properties of the aluminium(III)-hesperidin complex, for the determination of hesperidin in human plasma and pharmaceutical forms has been developed and validated. The complex shows a strong emission in the presence of the surfactant betain sulphonate SB 12 at 476 nm with excitation at 390 nm. The linearity range for pharmaceutical forms of hesperidin was 0.06-24.4 mu g mL(-1) with a limit of detection, LOD, of 0.016 mu g mL(-1) and a limit of quantification, LOQ, of 0.049 mu g mL(-1). Recovery values in the range 99.3-99.7 % indicate good accuracy of the method. A linear dependence of the intensity of fluorescence of the complex on the concentration of hesperidin in plasma was obtained in concentration range from 0.1-12.2 mu g mL(-1). The LOD was 0.032 mu g mL(-1) while LOQ was 0.096 mu g mL(-1). Recovery values were in the range 98.4-99.8 %. The reliability of the method was checked by an LC MS/MS method for plasma samples and an HPLC/UV method for tablets with direct determination of hesperidin after separation. Linearity range in determination of hesperidin in pharmaceutical forms was obtained in the range from 0.05 to 10.00 mu g mL(-1). The LOD was 0.01 mu g mL(-1) and the LOQ was 0.03 mu g mL(-1). The linearity range for the determination of hesperidin in plasma was 0.02-10.00 mu g mL(-1) with an LOD 0.005 mu g mL(-1) and an LOQ of 0.015 mu g mL(-1). The good agreement between the two methods indicates the usability of the proposed fluorometric method for the simple, precise and accurate determination of hesperidin in clinical and quality control laboratories.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms",
pages = "1640-1625",
number = "11",
volume = "77",
doi = "10.2298/JSC111005060P"
}

Pavun, L. A., Dimitrić-Marković, J., Djurdjević, P. T., Jelikic-Stankov, M. D., Đikanović, D., Ciric, A.,& Malesev, D. L.. (2012). Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 77(11), 1625-1640.
https://doi.org/10.2298/JSC111005060P

Pavun LA, Dimitrić-Marković J, Djurdjević PT, Jelikic-Stankov MD, Đikanović D, Ciric A, Malesev DL. Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms. in Journal of the Serbian Chemical Society. 2012;77(11):1625-1640.
doi:10.2298/JSC111005060P .

Pavun, Leposava A, Dimitrić-Marković, Jasmina, Djurdjević, Predrag T, Jelikic-Stankov, Milena D, Đikanović, Daniela, Ciric, Andrija, Malesev, Dusan L, "Development and validation of a fluorometric method for the determination of hesperidin in human plasma and pharmaceutical forms" in Journal of the Serbian Chemical Society, 77, no. 11 (2012):1625-1640,
https://doi.org/10.2298/JSC111005060P . .