TY  - JOUR
AU  - Bulajic, Aleksandra
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Djekic, I.
AU  - Krstic, B.
PY  - 2009
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/316
AB  - In Europe Phytophthora ramorum has been reported on a range of
ornamentals as well as on a limited number of tree species in at least in 11
countries: UK, Spain, Belgium, Germany, the Netherlands, Italy, France,
Switzerland, Poland, Slovenia, France and Norway (http://rapra.csl.gov.uk).
It is recorded mainly on Rhododendron but other hosts including Camellia,
Kalmia, Pieris and Viburnum are also affected. This pathogen is regarded
to be a potential source of inoculum for tree epidemics in Europe.
In June 2008, symptoms resembling those of P. ramorum were observed on
12 specimens of Rhododendron sp. cv. Baden – Baden in an open garden
in the vicinity of Zemun, Serbia. Plants with symptoms of leaf necrosis and
blight and petiole necrosis were sampled and examined. Pieces of leaf
tissue from the edge of lesions were surface-sterilized (70% ethanol) and
placed on carrot piece agar, CPA (Werres et al., 2001). After 4–7 days
incubation, isolates were slow growing with numerous semipapillate
caducous sporangia ((35–80 (54) × 17–32 (26) μm) and large chlamydospores (average diameter 48 μm). The mating type was determined as
A1, due to formation of typical sexual structures when crossed with A2
mating type of P. cinnamomi and P. cryptogea. The identity of the isolates
was confirmed by PCR using Phyto1/4 primer pair (Hayden et al., 2004).
A representative isolate (Pr92-08) was sequenced (GenBank Accession No.
EU915480) and the ITS sequence was identical to other P. ramorum
isolates on NCBI GenBank database.
Pathogenicity was confirmed by inoculation of non-wounded detached
leaves (Denman et al., 2005) of 15 different host plants. First necrotic spots
were noticeable after 3 day incubation in moist chamber in laboratory on
Rhododendron variegatum, R. morgenrot and Viburnum plicatum. The
control leaves, inoculated with sterile water, showed no reactions. In all
cases, the pathogen was successfully recovered from leaves with symptoms
and also detected using PCR.
This is the first report of P. ramorum on Rhododendron plants in Serbia.
The infected plants were destroyed and measures were taken to eradicate
the pathogen according to EU legislation. The discovery of infected
Rhododendron plants should prompt more detailed surveys, thorough
inspections and subsequent testing in other areas of Serbia.
PB  - Wiley-Blackwell, Hoboken
T2  - Plant Pathology
T1  - First report of Phytophthora ramorum on Rhododendron sp in Serbia
EP  - 804
IS  - 4
SP  - 804
VL  - 58
DO  - 10.1111/j.1365-3059.2009.02033.x
ER  - 

@article{
author = "Bulajic, Aleksandra and Jović, Jelena and Krnjajić, Slobodan and Djekic, I. and Krstic, B.",
year = "2009",
abstract = "In Europe Phytophthora ramorum has been reported on a range of
ornamentals as well as on a limited number of tree species in at least in 11
countries: UK, Spain, Belgium, Germany, the Netherlands, Italy, France,
Switzerland, Poland, Slovenia, France and Norway (http://rapra.csl.gov.uk).
It is recorded mainly on Rhododendron but other hosts including Camellia,
Kalmia, Pieris and Viburnum are also affected. This pathogen is regarded
to be a potential source of inoculum for tree epidemics in Europe.
In June 2008, symptoms resembling those of P. ramorum were observed on
12 specimens of Rhododendron sp. cv. Baden – Baden in an open garden
in the vicinity of Zemun, Serbia. Plants with symptoms of leaf necrosis and
blight and petiole necrosis were sampled and examined. Pieces of leaf
tissue from the edge of lesions were surface-sterilized (70% ethanol) and
placed on carrot piece agar, CPA (Werres et al., 2001). After 4–7 days
incubation, isolates were slow growing with numerous semipapillate
caducous sporangia ((35–80 (54) × 17–32 (26) μm) and large chlamydospores (average diameter 48 μm). The mating type was determined as
A1, due to formation of typical sexual structures when crossed with A2
mating type of P. cinnamomi and P. cryptogea. The identity of the isolates
was confirmed by PCR using Phyto1/4 primer pair (Hayden et al., 2004).
A representative isolate (Pr92-08) was sequenced (GenBank Accession No.
EU915480) and the ITS sequence was identical to other P. ramorum
isolates on NCBI GenBank database.
Pathogenicity was confirmed by inoculation of non-wounded detached
leaves (Denman et al., 2005) of 15 different host plants. First necrotic spots
were noticeable after 3 day incubation in moist chamber in laboratory on
Rhododendron variegatum, R. morgenrot and Viburnum plicatum. The
control leaves, inoculated with sterile water, showed no reactions. In all
cases, the pathogen was successfully recovered from leaves with symptoms
and also detected using PCR.
This is the first report of P. ramorum on Rhododendron plants in Serbia.
The infected plants were destroyed and measures were taken to eradicate
the pathogen according to EU legislation. The discovery of infected
Rhododendron plants should prompt more detailed surveys, thorough
inspections and subsequent testing in other areas of Serbia.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Plant Pathology",
title = "First report of Phytophthora ramorum on Rhododendron sp in Serbia",
pages = "804-804",
number = "4",
volume = "58",
doi = "10.1111/j.1365-3059.2009.02033.x"
}

Bulajic, A., Jović, J., Krnjajić, S., Djekic, I.,& Krstic, B.. (2009). First report of Phytophthora ramorum on Rhododendron sp in Serbia. in Plant Pathology
Wiley-Blackwell, Hoboken., 58(4), 804-804.
https://doi.org/10.1111/j.1365-3059.2009.02033.x

Bulajic A, Jović J, Krnjajić S, Djekic I, Krstic B. First report of Phytophthora ramorum on Rhododendron sp in Serbia. in Plant Pathology. 2009;58(4):804-804.
doi:10.1111/j.1365-3059.2009.02033.x .

Bulajic, Aleksandra, Jović, Jelena, Krnjajić, Slobodan, Djekic, I., Krstic, B., "First report of Phytophthora ramorum on Rhododendron sp in Serbia" in Plant Pathology, 58, no. 4 (2009):804-804,
https://doi.org/10.1111/j.1365-3059.2009.02033.x . .

TY  - JOUR
AU  - Bulajic, Aleksandra
AU  - Jović, Jelena
AU  - Krnjajić, Slobodan
AU  - Petrov, M.
AU  - Djekic, I.
AU  - Krstic, B.
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/296
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia
EP  - 1247
IS  - 8
SP  - 1247
VL  - 92
DO  - 10.1094/PDIS-92-8-1247A
ER  - 

@article{
author = "Bulajic, Aleksandra and Jović, Jelena and Krnjajić, Slobodan and Petrov, M. and Djekic, I. and Krstic, B.",
year = "2008",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia",
pages = "1247-1247",
number = "8",
volume = "92",
doi = "10.1094/PDIS-92-8-1247A"
}

Bulajic, A., Jović, J., Krnjajić, S., Petrov, M., Djekic, I.,& Krstic, B.. (2008). First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 92(8), 1247-1247.
https://doi.org/10.1094/PDIS-92-8-1247A

Bulajic A, Jović J, Krnjajić S, Petrov M, Djekic I, Krstic B. First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia. in Plant Disease. 2008;92(8):1247-1247.
doi:10.1094/PDIS-92-8-1247A .

Bulajic, Aleksandra, Jović, Jelena, Krnjajić, Slobodan, Petrov, M., Djekic, I., Krstic, B., "First report of Iris yellow spot virus on Onion (Allium cepa) in Serbia" in Plant Disease, 92, no. 8 (2008):1247-1247,
https://doi.org/10.1094/PDIS-92-8-1247A . .