TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1912
AB  - Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant 
for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also 
for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can 
be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most 
significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) 
causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing 
bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from 
different localities in Serbia, with the aim to examine their genetic features, through 
sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified 
with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained 
sequences were checked for homology with strains available in NCBI database. Multilocus 
sequence analysis (MLSA) was then performed to determine relatedness among the tested 
isolates. Based on six genes, tested isolates were identified as Xcc using NCBI BLAST, showing 
99-100% homology with the available data. Winter oilseed rape Xcc isolates were divided
into five groups on Neighbour-joining tree, revealing intrapathovar diversity within isolates
from this host. These results could be connected with pathogen adaptation to winter oilseed
rape as a new host, providing completely distinct ecological niche from B. oleracea vegetable
crops (broccoli, cabbage, cauliflower, etc.) which are common hosts for this bacteria.
This observation on Serbian Xcc isolates gives a significant input on genetic variation and
constitutes a highly informative sample of X. campestris diversity.
PB  - Serbian Genetic Society,  Belgrade, Serbia
C3  - 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
T1  - GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE
SP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1912
ER  - 

@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Marković, Sanja and Stanković, Slaviša and Popović, Tatjana",
year = "2019",
abstract = "Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant 
for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also 
for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can 
be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most 
significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) 
causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing 
bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from 
different localities in Serbia, with the aim to examine their genetic features, through 
sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified 
with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained 
sequences were checked for homology with strains available in NCBI database. Multilocus 
sequence analysis (MLSA) was then performed to determine relatedness among the tested 
isolates. Based on six genes, tested isolates were identified as Xcc using NCBI BLAST, showing 
99-100% homology with the available data. Winter oilseed rape Xcc isolates were divided
into five groups on Neighbour-joining tree, revealing intrapathovar diversity within isolates
from this host. These results could be connected with pathogen adaptation to winter oilseed
rape as a new host, providing completely distinct ecological niche from B. oleracea vegetable
crops (broccoli, cabbage, cauliflower, etc.) which are common hosts for this bacteria.
This observation on Serbian Xcc isolates gives a significant input on genetic variation and
constitutes a highly informative sample of X. campestris diversity.",
publisher = "Serbian Genetic Society,  Belgrade, Serbia",
journal = "6th CONGRESS OF THE SERBIAN GENETIC SOCIETY",
title = "GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE",
pages = "163",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1912"
}

Jelušić, A., Berić, T., Mitrović, P., Marković, S., Stanković, S.,& Popović, T.. (2019). GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
Serbian Genetic Society,  Belgrade, Serbia., 163.
https://hdl.handle.net/21.15107/rcub_rimsi_1912

Jelušić A, Berić T, Mitrović P, Marković S, Stanković S, Popović T. GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY. 2019;:163.
https://hdl.handle.net/21.15107/rcub_rimsi_1912 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Marković, Sanja, Stanković, Slaviša, Popović, Tatjana, "GENETIC DIVERSITY OF SERBIAN ISOLATES OF XANTHOMONAS CAMPESTRIS PV.  CAMPESTRIS ORIGINATED FROM WINTER OILSEED RAPE" in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY (2019):163,
https://hdl.handle.net/21.15107/rcub_rimsi_1912 .