TY  - CONF
AU  - Marković, Sanja
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Stanković, Slaviša
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1915
AB  - Blackleg disease, caused by pectolytic bacteria from genus Dickeya and Pectobacterium
is currently being one of the main concerns in potato production. Yield loss and reduced 
quality, visible through potato black rotting at the stem base, as well as whole plant decaying, 
makes potato cultivation less profitable. Blackleg disease outbreak was observed in potato 
field (cv. Lady Claire) in Bačka (northern Serbia) in June 2018. The percent of the infected 
plants, estimated during the vegetation season was 45%, with yield reduction of about 20%. 
In this study the causal pathogens were genetically characterized using multilocus sequence 
analysis (MLSA). DNA of the obtained isolates was amplified with primers made based on 
the sequences of five housekeeping genes - gapA, icdA, mdh, pgi and proA and sequenced. 
Phylogenetic analysis was performed to compare the obtained isolates with the ones already 
deposited in NCBI database. NCBI BLAST identified isolates as Dickeya dianthicola and 
Pectobacterium carotovorum subsp. brasiliensis, indicating on the persistence of mix infection 
on observed potato crop. According to all genes, isolates appeared to be the most similar 
(99-100%) to the reference strains - D. dianthicola (CFBP 1200) and P. carotovorum subsp. 
brasiliensis (BC1). Blast results were confirmed after phylogenetic analysis, where isolates 
of both identified species were clustering with the rest of the strains of the same species, 
obtained from NCBI. Our findings confirm the presence of new blackleg causal agents, D. 
dianthicola and P. carotovorum subsp. brasiliensis on potato in Serbia. Their isolation and 
localization is of great importance, especially D. dianthicola which has quarantine status, and 
therefore it gives a signal for pathogen eradication and prevention of its future spreading.
PB  - Serbian Genetic Society,  Belgrade, Serbia
C3  - 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
T1  - GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO
SP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1915
ER  - 

@conference{
author = "Marković, Sanja and Popović, Tatjana and Jelušić, Aleksandra and Iličić, Renata and Stanković, Slaviša",
year = "2019",
abstract = "Blackleg disease, caused by pectolytic bacteria from genus Dickeya and Pectobacterium
is currently being one of the main concerns in potato production. Yield loss and reduced 
quality, visible through potato black rotting at the stem base, as well as whole plant decaying, 
makes potato cultivation less profitable. Blackleg disease outbreak was observed in potato 
field (cv. Lady Claire) in Bačka (northern Serbia) in June 2018. The percent of the infected 
plants, estimated during the vegetation season was 45%, with yield reduction of about 20%. 
In this study the causal pathogens were genetically characterized using multilocus sequence 
analysis (MLSA). DNA of the obtained isolates was amplified with primers made based on 
the sequences of five housekeeping genes - gapA, icdA, mdh, pgi and proA and sequenced. 
Phylogenetic analysis was performed to compare the obtained isolates with the ones already 
deposited in NCBI database. NCBI BLAST identified isolates as Dickeya dianthicola and 
Pectobacterium carotovorum subsp. brasiliensis, indicating on the persistence of mix infection 
on observed potato crop. According to all genes, isolates appeared to be the most similar 
(99-100%) to the reference strains - D. dianthicola (CFBP 1200) and P. carotovorum subsp. 
brasiliensis (BC1). Blast results were confirmed after phylogenetic analysis, where isolates 
of both identified species were clustering with the rest of the strains of the same species, 
obtained from NCBI. Our findings confirm the presence of new blackleg causal agents, D. 
dianthicola and P. carotovorum subsp. brasiliensis on potato in Serbia. Their isolation and 
localization is of great importance, especially D. dianthicola which has quarantine status, and 
therefore it gives a signal for pathogen eradication and prevention of its future spreading.",
publisher = "Serbian Genetic Society,  Belgrade, Serbia",
journal = "6th CONGRESS OF THE SERBIAN GENETIC SOCIETY",
title = "GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO",
pages = "165",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1915"
}

Marković, S., Popović, T., Jelušić, A., Iličić, R.,& Stanković, S.. (2019). GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
Serbian Genetic Society,  Belgrade, Serbia., 165.
https://hdl.handle.net/21.15107/rcub_rimsi_1915

Marković S, Popović T, Jelušić A, Iličić R, Stanković S. GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY. 2019;:165.
https://hdl.handle.net/21.15107/rcub_rimsi_1915 .

Marković, Sanja, Popović, Tatjana, Jelušić, Aleksandra, Iličić, Renata, Stanković, Slaviša, "GENETIC INSIGHT INTO THE ISOLATES CAUSING BLACKLEG DISEASE ON POTATO" in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY (2019):165,
https://hdl.handle.net/21.15107/rcub_rimsi_1915 .