TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Berić, Tanja
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1933
AB  - Background: Oilseed rape (Brassica napus) is an oil-producing crop, significant for vegetable oil and 
biodiesel production. The phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) 
causing bacterial leaf blight was recorded for a first time in 2010 on self-growing winter oilseed rape 
in Serbia. Ever since, it continued appearing on cultivated crops too. 
Objectives: The aim of this research was to reveal genetic heterogeneity among 25 Xcc isolates, 
originated from different cultivars of winter oilseed rape obtained in nine-year period (2010-2018) 
from different localities in Vojvodina region (Serbia) using repetitive sequence-based PCR (rep-PCR) 
fingerprinting. 
Methods: Genomic DNA was extracted using CTAB method. Specific primer pair XCF/XCR, amplifying 
a DNA fragment of hrpF gene from X. campestris was used for rapid preliminary identification of all 
tested isolates. Rep-PCR using BOX, ERIC and REP-PCR primers was used to detect presence of 
genetic heterogeneity among Xcc isolates.
Results: All 25 tested isolates were preliminary identified as Xcc based on a positive PCR reaction 
after amplification of 525 bp region using XCF/XCR primers. BOX and ERIC-PCR generated seventeen 
different DNA fingerprinting profiles, unlike REP-PCR where only nine different profiles were 
detected. These results show presence of genetic polymorphism among Xcc isolates from winter 
oilseed rape, which could indicate either their affiliation to some of the identified Xcc races, or 
formation of a new Xcc ecotype.
PB  - Federation of European Microbiological Societies
C3  - 8th Congress of  European Microbiologists
T1  - Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques
SP  - 991
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1933
ER  - 

@conference{
author = "Jelušić, Aleksandra and Popović, Tatjana and Mitrović, Petar and Dimkić, Ivica and Stanković, Slaviša and Marković, Sanja and Berić, Tanja",
year = "2019",
abstract = "Background: Oilseed rape (Brassica napus) is an oil-producing crop, significant for vegetable oil and 
biodiesel production. The phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) 
causing bacterial leaf blight was recorded for a first time in 2010 on self-growing winter oilseed rape 
in Serbia. Ever since, it continued appearing on cultivated crops too. 
Objectives: The aim of this research was to reveal genetic heterogeneity among 25 Xcc isolates, 
originated from different cultivars of winter oilseed rape obtained in nine-year period (2010-2018) 
from different localities in Vojvodina region (Serbia) using repetitive sequence-based PCR (rep-PCR) 
fingerprinting. 
Methods: Genomic DNA was extracted using CTAB method. Specific primer pair XCF/XCR, amplifying 
a DNA fragment of hrpF gene from X. campestris was used for rapid preliminary identification of all 
tested isolates. Rep-PCR using BOX, ERIC and REP-PCR primers was used to detect presence of 
genetic heterogeneity among Xcc isolates.
Results: All 25 tested isolates were preliminary identified as Xcc based on a positive PCR reaction 
after amplification of 525 bp region using XCF/XCR primers. BOX and ERIC-PCR generated seventeen 
different DNA fingerprinting profiles, unlike REP-PCR where only nine different profiles were 
detected. These results show presence of genetic polymorphism among Xcc isolates from winter 
oilseed rape, which could indicate either their affiliation to some of the identified Xcc races, or 
formation of a new Xcc ecotype.",
publisher = "Federation of European Microbiological Societies",
journal = "8th Congress of  European Microbiologists",
title = "Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques",
pages = "991",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1933"
}

Jelušić, A., Popović, T., Mitrović, P., Dimkić, I., Stanković, S., Marković, S.,& Berić, T.. (2019). Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques. in 8th Congress of  European Microbiologists
Federation of European Microbiological Societies., 991.
https://hdl.handle.net/21.15107/rcub_rimsi_1933

Jelušić A, Popović T, Mitrović P, Dimkić I, Stanković S, Marković S, Berić T. Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques. in 8th Congress of  European Microbiologists. 2019;:991.
https://hdl.handle.net/21.15107/rcub_rimsi_1933 .

Jelušić, Aleksandra, Popović, Tatjana, Mitrović, Petar, Dimkić, Ivica, Stanković, Slaviša, Marković, Sanja, Berić, Tanja, "Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques" in 8th Congress of  European Microbiologists (2019):991,
https://hdl.handle.net/21.15107/rcub_rimsi_1933 .