@article{
author = "Milošević, Ivan and Radovanovic, Anita and Danilović Luković, Jelena and Luzajic Bozinovski, Tijana and Sourice-Petit, Sophie and Beck-Cormier, Sarah and Guicheux, Jerome and Kovačević Filipović, Milica",
year = "2018",
abstract = "Maternal hypothyroidism in its overt form affects skeletal development of the offspring,
but these data are not available for the subclinical form which is becoming very frequent
among pregnant women. We hypothesized that the subclinical form of hypothyroidism
in rat dams, influences the process of offspring endochondral ossification affecting
proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups.
Seven-day-old male pups (n=18) derived from control dams and dams treated with a
low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water
during pregnancy and lactation were used. Histomorphometric analysis of pups’ tibia
proximal growth plate, expression of mRNA, immunohistochemical and histochemical
visualization of extracellular matrix components was performed. The length of the tibia
was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical
and overt form were lower while the amount of glycosaminoglycans was higher when
compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA
indicated altered osteoclasts function while lower expression of dentin matrix acid
protein–1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised
bone formation in the overt form of hypothyroidism. The subclinical form of maternal
hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes
and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism
had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in
growth plate formation.",
publisher = "Faculty of Veterinary Medicine, University of Belgrade, Serbia",
journal = "Acta Veterinaria-Beograd",
title = "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation",
pages = "320-301",
number = "3",
volume = "68",
doi = "10.2478/acve-2018-0026"
}