Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat
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2015
Authors
Kostić Kravljanac, LjiljanaNikolić, Nina
Samardžić, Jelena T.
Milisavljević, Mira
Maksimović, Vuk
Cakmak, Dragan
Manojlović, Dragan
Nikolic, Miroslav
Article (Published version)
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We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were elim...inated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).
Keywords:
Wheat / Root exudates / Rhizosphere / Polluted acid soil / Phosphorus deficiency / LimingSource:
Biology and Fertility of Soils, 2015, 51, 3, 289-298Publisher:
- Springer, New York
Funding / projects:
- Mineral Stress and Plant Adaptations to Marginal Agricultural Soils (RS-MESTD-Basic Research (BR or ON)-173028)
- The Role of Transcription Factors and Small RNAs in Abiotic Stress Response in Plants and Genetic Diversity of Plant Species Important for Agriculture and Biotechnology (RS-MESTD-Basic Research (BR or ON)-173005)
DOI: 10.1007/s00374-014-0975-y
ISSN: 0178-2762
WoS: 000351197700002
Scopus: 2-s2.0-84925484391
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Institut za multidisciplinarna istraživanjaTY - JOUR AU - Kostić Kravljanac, Ljiljana AU - Nikolić, Nina AU - Samardžić, Jelena T. AU - Milisavljević, Mira AU - Maksimović, Vuk AU - Cakmak, Dragan AU - Manojlović, Dragan AU - Nikolic, Miroslav PY - 2015 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/838 AB - We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1). PB - Springer, New York T2 - Biology and Fertility of Soils T1 - Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat EP - 298 IS - 3 SP - 289 VL - 51 DO - 10.1007/s00374-014-0975-y ER -
@article{ author = "Kostić Kravljanac, Ljiljana and Nikolić, Nina and Samardžić, Jelena T. and Milisavljević, Mira and Maksimović, Vuk and Cakmak, Dragan and Manojlović, Dragan and Nikolic, Miroslav", year = "2015", abstract = "We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and P-i transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).", publisher = "Springer, New York", journal = "Biology and Fertility of Soils", title = "Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat", pages = "298-289", number = "3", volume = "51", doi = "10.1007/s00374-014-0975-y" }
Kostić Kravljanac, L., Nikolić, N., Samardžić, J. T., Milisavljević, M., Maksimović, V., Cakmak, D., Manojlović, D.,& Nikolic, M.. (2015). Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat. in Biology and Fertility of Soils Springer, New York., 51(3), 289-298. https://doi.org/10.1007/s00374-014-0975-y
Kostić Kravljanac L, Nikolić N, Samardžić JT, Milisavljević M, Maksimović V, Cakmak D, Manojlović D, Nikolic M. Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat. in Biology and Fertility of Soils. 2015;51(3):289-298. doi:10.1007/s00374-014-0975-y .
Kostić Kravljanac, Ljiljana, Nikolić, Nina, Samardžić, Jelena T., Milisavljević, Mira, Maksimović, Vuk, Cakmak, Dragan, Manojlović, Dragan, Nikolic, Miroslav, "Liming of anthropogenically acidified soil promotes phosphorus acquisition in the rhizosphere of wheat" in Biology and Fertility of Soils, 51, no. 3 (2015):289-298, https://doi.org/10.1007/s00374-014-0975-y . .