Extracellular iron diminishes anticancer effects of vitamin C: An in vitro study
2014
Autori
Mojic, MarijaBogdanović Pristov, Jelena
Maksimović-Ivanić, Danijela
Jones, David R
Stanić, Marina
Mijatović, Sanja
Spasojević, Ivan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In vitro studies have shown that hydrogen peroxide (H2O2) produced by high-concentration ascorbate and cell culture medium iron efficiently kills cancer cells. This provided the rationale for clinical trials of high-dose intravenous ascorbate-based treatment for cancer. A drawback in all the in vitro studies was their failure to take into account the in vivo concentration of iron to supplement cell culture media which are characterized by low iron content. Here we showed, using two prostate cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the anticancer/cytotoxic effects of ascorbate are completely abolished by iron at physiological concentrations in cell culture medium and human plasma. A detailed examination of mechanisms showed that iron at physiological concentrations promotes both production and decomposition of H2O2. The latter is mediated by Fenton reaction and prevents H2O2 accumulation. The hydroxyl radical, which is produced in the Fenton reaction, is buffered ...by extracellular proteins, and could not affect intracellular targets like H2O2. These findings show that anticancer effects of ascorbate have been significantly overestimated in previous in vitro studies, and that common cell culture media might be unsuitable for redox research.
Ključne reči:
hydrogen peroxide / ascorbate / Fenton reaction / prostate cancer cell lines / EPRIzvor:
Scientific Reports, 2014, 4Izdavač:
- Nature Publishing Group, London
Finansiranje / projekti:
- Molekularni mehanizmi fiziološke i farmakološke kontrole inflamacije i kancera (RS-MESTD-Basic Research (BR or ON)-173013)
- Molekularni mehanizmi redoks signalinga u homeostazi, adaptaciji i patologiji (RS-MESTD-Basic Research (BR or ON)-173014)
DOI: 10.1038/srep05955
ISSN: 2045-2322
PubMed: 25092529
WoS: 000340032100004
Scopus: 2-s2.0-84905454521
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Mojic, Marija AU - Bogdanović Pristov, Jelena AU - Maksimović-Ivanić, Danijela AU - Jones, David R AU - Stanić, Marina AU - Mijatović, Sanja AU - Spasojević, Ivan PY - 2014 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/821 AB - In vitro studies have shown that hydrogen peroxide (H2O2) produced by high-concentration ascorbate and cell culture medium iron efficiently kills cancer cells. This provided the rationale for clinical trials of high-dose intravenous ascorbate-based treatment for cancer. A drawback in all the in vitro studies was their failure to take into account the in vivo concentration of iron to supplement cell culture media which are characterized by low iron content. Here we showed, using two prostate cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the anticancer/cytotoxic effects of ascorbate are completely abolished by iron at physiological concentrations in cell culture medium and human plasma. A detailed examination of mechanisms showed that iron at physiological concentrations promotes both production and decomposition of H2O2. The latter is mediated by Fenton reaction and prevents H2O2 accumulation. The hydroxyl radical, which is produced in the Fenton reaction, is buffered by extracellular proteins, and could not affect intracellular targets like H2O2. These findings show that anticancer effects of ascorbate have been significantly overestimated in previous in vitro studies, and that common cell culture media might be unsuitable for redox research. PB - Nature Publishing Group, London T2 - Scientific Reports T1 - Extracellular iron diminishes anticancer effects of vitamin C: An in vitro study VL - 4 DO - 10.1038/srep05955 ER -
@article{ author = "Mojic, Marija and Bogdanović Pristov, Jelena and Maksimović-Ivanić, Danijela and Jones, David R and Stanić, Marina and Mijatović, Sanja and Spasojević, Ivan", year = "2014", abstract = "In vitro studies have shown that hydrogen peroxide (H2O2) produced by high-concentration ascorbate and cell culture medium iron efficiently kills cancer cells. This provided the rationale for clinical trials of high-dose intravenous ascorbate-based treatment for cancer. A drawback in all the in vitro studies was their failure to take into account the in vivo concentration of iron to supplement cell culture media which are characterized by low iron content. Here we showed, using two prostate cancer cell lines (LNCaP and PC-3) and primary astrocytes, that the anticancer/cytotoxic effects of ascorbate are completely abolished by iron at physiological concentrations in cell culture medium and human plasma. A detailed examination of mechanisms showed that iron at physiological concentrations promotes both production and decomposition of H2O2. The latter is mediated by Fenton reaction and prevents H2O2 accumulation. The hydroxyl radical, which is produced in the Fenton reaction, is buffered by extracellular proteins, and could not affect intracellular targets like H2O2. These findings show that anticancer effects of ascorbate have been significantly overestimated in previous in vitro studies, and that common cell culture media might be unsuitable for redox research.", publisher = "Nature Publishing Group, London", journal = "Scientific Reports", title = "Extracellular iron diminishes anticancer effects of vitamin C: An in vitro study", volume = "4", doi = "10.1038/srep05955" }
Mojic, M., Bogdanović Pristov, J., Maksimović-Ivanić, D., Jones, D. R., Stanić, M., Mijatović, S.,& Spasojević, I.. (2014). Extracellular iron diminishes anticancer effects of vitamin C: An in vitro study. in Scientific Reports Nature Publishing Group, London., 4. https://doi.org/10.1038/srep05955
Mojic M, Bogdanović Pristov J, Maksimović-Ivanić D, Jones DR, Stanić M, Mijatović S, Spasojević I. Extracellular iron diminishes anticancer effects of vitamin C: An in vitro study. in Scientific Reports. 2014;4. doi:10.1038/srep05955 .
Mojic, Marija, Bogdanović Pristov, Jelena, Maksimović-Ivanić, Danijela, Jones, David R, Stanić, Marina, Mijatović, Sanja, Spasojević, Ivan, "Extracellular iron diminishes anticancer effects of vitamin C: An in vitro study" in Scientific Reports, 4 (2014), https://doi.org/10.1038/srep05955 . .