Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce
2014
Autori
Tadic, VojinPetric, Marija
Milošević, Snežana
Cingel, Aleksandar
Raspor, Martin
Spasojević, Dragica
Tadic, Jovan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this study we examined the activities of polyphenol oxidase (PPO) and antioxidant enzymes, peroxidase (POX) and catalase (CAT) during lettuce seed germination at different concentrations of phenol. Out of eleven varieties of lettuce, four were chosen according to their germination tolerance to phenol as follows: plants exhibiting high (Ljubljanska ledenka - LJL and Nansen - N) and low toleranace (Little Gem - LG and Majska kraljica - MK). A decrease in germination efficiency after exposure to LD50 of phenol was determined for these four varieties. The effects of phenol treatment on POX, CAT and PPO activities were determined after 4, 5, 6, 7 and 8 days of growth at LD50 concentrations. A trend of increased peroxidase activity was observed in seeds grown on LD50 of phenol compared to control seeds. A significant increase in CAT activity was observed at the beginning of treatment for MK, LG and N in seeds grown on phenol as well as in control seeds. A trend of increased PPO activity w...as observed in all control seeds. We also investigated the affinity of PPO for two different substrates that were used for the determination of enzyme activity. Our results show that LJL and N are the varieties most tolerant to growth on phenol. Here we report on the activities of their antioxidant enzymes and PPO during seed germination.
Ključne reči:
polyphenol oxidase / phenol / Lettuce seeds / antioxidant enzymesIzvor:
Archives of Biological Sciences, 2014, 66, 4, 1503-1514Izdavač:
- Srpsko biološko društvo, Beograd, i dr.
Finansiranje / projekti:
DOI: 10.2298/ABS1404503T
ISSN: 0354-4664
WoS: 000346477900025
Scopus: 2-s2.0-84921653315
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Tadic, Vojin AU - Petric, Marija AU - Milošević, Snežana AU - Cingel, Aleksandar AU - Raspor, Martin AU - Spasojević, Dragica AU - Tadic, Jovan PY - 2014 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/800 AB - In this study we examined the activities of polyphenol oxidase (PPO) and antioxidant enzymes, peroxidase (POX) and catalase (CAT) during lettuce seed germination at different concentrations of phenol. Out of eleven varieties of lettuce, four were chosen according to their germination tolerance to phenol as follows: plants exhibiting high (Ljubljanska ledenka - LJL and Nansen - N) and low toleranace (Little Gem - LG and Majska kraljica - MK). A decrease in germination efficiency after exposure to LD50 of phenol was determined for these four varieties. The effects of phenol treatment on POX, CAT and PPO activities were determined after 4, 5, 6, 7 and 8 days of growth at LD50 concentrations. A trend of increased peroxidase activity was observed in seeds grown on LD50 of phenol compared to control seeds. A significant increase in CAT activity was observed at the beginning of treatment for MK, LG and N in seeds grown on phenol as well as in control seeds. A trend of increased PPO activity was observed in all control seeds. We also investigated the affinity of PPO for two different substrates that were used for the determination of enzyme activity. Our results show that LJL and N are the varieties most tolerant to growth on phenol. Here we report on the activities of their antioxidant enzymes and PPO during seed germination. PB - Srpsko biološko društvo, Beograd, i dr. T2 - Archives of Biological Sciences T1 - Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce EP - 1514 IS - 4 SP - 1503 VL - 66 DO - 10.2298/ABS1404503T ER -
@article{ author = "Tadic, Vojin and Petric, Marija and Milošević, Snežana and Cingel, Aleksandar and Raspor, Martin and Spasojević, Dragica and Tadic, Jovan", year = "2014", abstract = "In this study we examined the activities of polyphenol oxidase (PPO) and antioxidant enzymes, peroxidase (POX) and catalase (CAT) during lettuce seed germination at different concentrations of phenol. Out of eleven varieties of lettuce, four were chosen according to their germination tolerance to phenol as follows: plants exhibiting high (Ljubljanska ledenka - LJL and Nansen - N) and low toleranace (Little Gem - LG and Majska kraljica - MK). A decrease in germination efficiency after exposure to LD50 of phenol was determined for these four varieties. The effects of phenol treatment on POX, CAT and PPO activities were determined after 4, 5, 6, 7 and 8 days of growth at LD50 concentrations. A trend of increased peroxidase activity was observed in seeds grown on LD50 of phenol compared to control seeds. A significant increase in CAT activity was observed at the beginning of treatment for MK, LG and N in seeds grown on phenol as well as in control seeds. A trend of increased PPO activity was observed in all control seeds. We also investigated the affinity of PPO for two different substrates that were used for the determination of enzyme activity. Our results show that LJL and N are the varieties most tolerant to growth on phenol. Here we report on the activities of their antioxidant enzymes and PPO during seed germination.", publisher = "Srpsko biološko društvo, Beograd, i dr.", journal = "Archives of Biological Sciences", title = "Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce", pages = "1514-1503", number = "4", volume = "66", doi = "10.2298/ABS1404503T" }
Tadic, V., Petric, M., Milošević, S., Cingel, A., Raspor, M., Spasojević, D.,& Tadic, J.. (2014). Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce. in Archives of Biological Sciences Srpsko biološko društvo, Beograd, i dr.., 66(4), 1503-1514. https://doi.org/10.2298/ABS1404503T
Tadic V, Petric M, Milošević S, Cingel A, Raspor M, Spasojević D, Tadic J. Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce. in Archives of Biological Sciences. 2014;66(4):1503-1514. doi:10.2298/ABS1404503T .
Tadic, Vojin, Petric, Marija, Milošević, Snežana, Cingel, Aleksandar, Raspor, Martin, Spasojević, Dragica, Tadic, Jovan, "Effect of phenol on germination capacity and polyphenol oxidase, peroxidase and catalase activities in lettuce" in Archives of Biological Sciences, 66, no. 4 (2014):1503-1514, https://doi.org/10.2298/ABS1404503T . .